##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename lane8_Undetermined_L008_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 101558750 Sequences flagged as poor quality 0 Sequence length 93 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.58040874863072 33.0 33.0 33.0 33.0 33.0 2 32.66784912181373 33.0 33.0 33.0 33.0 33.0 3 32.72596465592576 33.0 33.0 33.0 33.0 33.0 4 36.66272269991507 37.0 37.0 37.0 37.0 37.0 5 36.52156483808633 37.0 37.0 37.0 37.0 37.0 6 36.529893160362846 37.0 37.0 37.0 37.0 37.0 7 36.482542459413885 37.0 37.0 37.0 37.0 37.0 8 36.50607753640134 37.0 37.0 37.0 37.0 37.0 9 36.51334325205854 37.0 37.0 37.0 37.0 37.0 10-11 36.498534803746594 37.0 37.0 37.0 37.0 37.0 12-13 36.50302387041983 37.0 37.0 37.0 37.0 37.0 14-15 38.619100643716074 40.0 37.0 40.0 37.0 40.0 16-17 38.58557560032986 40.0 37.0 40.0 37.0 40.0 18-19 38.57019335113912 40.0 37.0 40.0 37.0 40.0 20-21 38.538512378303196 40.0 37.0 40.0 37.0 40.0 22-23 38.47582779425702 40.0 37.0 40.0 37.0 40.0 24-25 38.407858274151664 40.0 37.0 40.0 37.0 40.0 26-27 38.322614033748934 40.0 37.0 40.0 37.0 40.0 28-29 38.17527500092311 40.0 37.0 40.0 37.0 40.0 30-31 38.13424258864943 40.0 37.0 40.0 37.0 40.0 32-33 38.04628888205103 40.0 37.0 40.0 37.0 40.0 34-35 37.980364582076874 40.0 37.0 40.0 35.0 40.0 36-37 37.92503405664209 40.0 37.0 40.0 33.0 40.0 38-39 37.806628153654906 40.0 37.0 40.0 33.0 40.0 40-41 37.69837471414329 40.0 37.0 40.0 33.0 40.0 42-43 37.63514913289106 40.0 37.0 40.0 33.0 40.0 44-45 37.50886997919923 40.0 37.0 40.0 33.0 40.0 46-47 37.37586502393935 40.0 37.0 40.0 33.0 40.0 48-49 37.23628645488449 40.0 37.0 40.0 33.0 40.0 50-51 37.08965164498382 40.0 37.0 40.0 33.0 40.0 52-53 37.09731174812608 40.0 37.0 40.0 33.0 40.0 54-55 36.93515066402452 37.0 37.0 40.0 33.0 40.0 56-57 36.73015835661624 37.0 37.0 40.0 33.0 40.0 58-59 36.542721478946916 37.0 37.0 40.0 33.0 40.0 60-61 36.31851993550531 37.0 37.0 40.0 33.0 40.0 62-63 36.12684542198481 37.0 37.0 40.0 33.0 40.0 64-65 35.909404861717974 37.0 37.0 40.0 33.0 40.0 66-67 35.68694350314473 37.0 35.0 40.0 33.0 40.0 68-69 35.43737041367682 37.0 33.0 40.0 27.0 40.0 70-71 35.228199495365985 37.0 33.0 37.0 27.0 40.0 72-73 34.946472170049354 37.0 33.0 37.0 27.0 40.0 74-75 34.60703457850751 37.0 33.0 37.0 27.0 40.0 76-77 33.25720581436853 35.0 33.0 37.0 27.0 37.0 78-79 34.35282956909178 37.0 33.0 37.0 27.0 37.0 80-81 34.42469565645501 37.0 33.0 37.0 27.0 37.0 82-83 34.268763233103996 37.0 33.0 37.0 27.0 37.0 84-85 34.09232448705798 37.0 33.0 37.0 27.0 37.0 86-87 33.91904188462343 37.0 33.0 37.0 27.0 37.0 88-89 33.796341536302876 37.0 33.0 37.0 27.0 37.0 90-91 33.55445238347262 37.0 33.0 37.0 27.0 37.0 92-93 32.434817723731335 35.0 33.0 37.0 24.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2304 1 0.217565558506152 2304 2 0.17858875918083328 2304 3 0.14509381005530742 2304 4 0.2216436771780721 2304 5 0.19943869236871592 2304 6 0.20009759643720315 2304 7 0.1982927155116414 2304 8 0.21688065831783376 2304 9 0.24128305877371758 2304 10-11 0.2975880105623574 2304 12-13 0.27636607217069553 2304 14-15 0.5050013879460806 2304 16-17 0.5094452556664564 2304 18-19 0.46381582324065107 2304 20-21 0.4158183442936476 2304 22-23 0.45460217506747114 2304 24-25 0.4387561227993402 2304 26-27 0.4346775829038094 2304 28-29 0.3667289860546603 2304 30-31 0.37480592252377676 2304 32-33 0.3494298045881834 2304 34-35 0.3313502126170391 2304 36-37 0.29320231996837975 2304 38-39 0.33032367258972073 2304 40-41 0.24963582789574446 2304 42-43 0.2412969857524132 2304 44-45 0.23423170839420493 2304 46-47 0.2597967427211998 2304 48-49 0.25120167833031104 2304 50-51 0.2292825179434459 2304 52-53 0.24864872388092607 2304 54-55 0.19413497775205002 2304 56-57 0.2279268677951123 2304 58-59 0.22647596177033336 2304 60-61 0.1705959501138139 2304 62-63 0.1392376995088611 2304 64-65 0.20466856874267592 2304 66-67 0.16379329435331869 2304 68-69 0.10301572899048494 2304 70-71 0.14839484752131682 2304 72-73 0.1711753155747715 2304 74-75 0.10442924631302475 2304 76-77 0.1115728405204095 2304 78-79 0.318938007477918 2304 80-81 0.39971635294758556 2304 82-83 0.3670853854785108 2304 84-85 0.33777061774753037 2304 86-87 0.38442588840072744 2304 88-89 0.3387305584325446 2304 90-91 0.45121851093131227 2304 92-93 0.5521251662808737 2305 1 0.23749859290560238 2305 2 0.19793728253331722 2305 3 0.16517384332681218 2305 4 0.23080238544823573 2305 5 0.2290349661221711 2305 6 0.21900564720375115 2305 7 0.24266675671526627 2305 8 0.24596735075108 2305 9 0.22597589788352224 2305 10-11 0.28400954204165174 2305 12-13 0.2786406109703705 2305 14-15 0.46293466980831965 2305 16-17 0.49191697708064197 2305 18-19 0.4306878311507987 2305 20-21 0.41277973390169365 2305 22-23 0.49238884351964884 2305 24-25 0.4621922208550089 2305 26-27 0.4838720339445217 2305 28-29 0.5277709306872467 2305 30-31 0.5221554040745175 2305 32-33 0.5208202823556505 2305 34-35 0.5122743143999813 2305 36-37 0.5118491813000361 2305 38-39 0.5066708859976501 2305 40-41 0.4847849188820277 2305 42-43 0.4027026007654655 2305 44-45 0.4675567104264218 2305 46-47 0.47628310298386367 2305 48-49 0.4504924135242945 2305 50-51 0.47708516605278106 2305 52-53 0.4630142483852566 2305 54-55 0.47442016215917704 2305 56-57 0.5239021784576394 2305 58-59 0.5571005573911165 2305 60-61 0.48894044933357605 2305 62-63 0.4884012566894498 2305 64-65 0.5005362030357077 2305 66-67 0.5097490026130416 2305 68-69 0.40504260558994076 2305 70-71 0.4504187698554105 2305 72-73 0.4288764591825185 2305 74-75 0.3924055959440196 2305 76-77 0.43146658833196483 2305 78-79 0.5382991891582165 2305 80-81 0.6266059677816216 2305 82-83 0.6759680663140344 2305 84-85 0.6642798502237 2305 86-87 0.6909150388515286 2305 88-89 0.704791140885078 2305 90-91 0.7077331261482129 2305 92-93 0.78836064207308 2306 1 0.2059973648475193 2306 2 0.18668403934582756 2306 3 0.16418981830378954 2306 4 0.21528821730837677 2306 5 0.1849917195309061 2306 6 0.19028571055854115 2306 7 0.2208430798713934 2306 8 0.23594668452286527 2306 9 0.22567182930593788 2306 10-11 0.2759980256710364 2306 12-13 0.273841452404298 2306 14-15 0.4369063704009406 2306 16-17 0.46729504390296484 2306 18-19 0.4464812996334331 2306 20-21 0.4016421460006754 2306 22-23 0.39652924951541735 2306 24-25 0.38794841089013943 2306 26-27 0.4478015041132366 2306 28-29 0.45239388343949116 2306 30-31 0.47746178707148346 2306 32-33 0.4169137622486758 2306 34-35 0.42055467854861917 2306 36-37 0.3880878984733229 2306 38-39 0.4850862652475385 2306 40-41 0.44424539365692794 2306 42-43 0.41398983046081383 2306 44-45 0.36149036521760536 2306 46-47 0.46246663608317107 2306 48-49 0.45683739426172565 2306 50-51 0.37284679112154606 2306 52-53 0.4278154326484014 2306 54-55 0.4227754858356576 2306 56-57 0.4861886512316005 2306 58-59 0.46264821592276206 2306 60-61 0.3372633735506625 2306 62-63 0.32888679987587466 2306 64-65 0.36882339026799826 2306 66-67 0.38981519681006205 2306 68-69 0.3786190451205016 2306 70-71 0.33207420483271477 2306 72-73 0.30340072158175246 2306 74-75 0.28628594776173344 2306 76-77 0.2972816455926903 2306 78-79 0.4953463202618522 2306 80-81 0.5806530178918408 2306 82-83 0.5967189032050939 2306 84-85 0.5941375190734774 2306 86-87 0.602332695648947 2306 88-89 0.6387899373986272 2306 90-91 0.6969614361826899 2306 92-93 0.7042899290667606 2307 1 0.19965168250585918 2307 2 0.17139034690235633 2307 3 0.1370382871999425 2307 4 0.21365156446855593 2307 5 0.20253390402173466 2307 6 0.21134482015509803 2307 7 0.2265978322729154 2307 8 0.2194503005136923 2307 9 0.25236677475233904 2307 10-11 0.25553585030576187 2307 12-13 0.24343203134509395 2307 14-15 0.4009704906925293 2307 16-17 0.4133507221593504 2307 18-19 0.38775822019743345 2307 20-21 0.353286161462826 2307 22-23 0.4284857762604375 2307 24-25 0.41936213992527627 2307 26-27 0.4590986993609789 2307 28-29 0.3897777041925181 2307 30-31 0.40977338197446755 2307 32-33 0.39897510381970136 2307 34-35 0.3802149496043157 2307 36-37 0.37905836494913103 2307 38-39 0.3969580283956944 2307 40-41 0.3378965968605385 2307 42-43 0.2942646182444477 2307 44-45 0.2955955058916331 2307 46-47 0.34047001650370845 2307 48-49 0.3029701916853327 2307 50-51 0.3322736230771355 2307 52-53 0.25462986755246675 2307 54-55 0.262349251856115 2307 56-57 0.26564914122438665 2307 58-59 0.26128719089470565 2307 60-61 0.22286673051706174 2307 62-63 0.12646640121392494 2307 64-65 0.12862977894904049 2307 66-67 0.1394115654724004 2307 68-69 0.21240151347034697 2307 70-71 0.18628119865911685 2307 72-73 0.10926168583910112 2307 74-75 0.10573845811531868 2307 76-77 0.07368231150321947 2307 78-79 0.2465055913172236 2307 80-81 0.3348193951500136 2307 82-83 0.35300364305374643 2307 84-85 0.32355888253385245 2307 86-87 0.32728265213118135 2307 88-89 0.2896950770389708 2307 90-91 0.3404976125549979 2307 92-93 0.4276931040450336 2308 1 0.16355595662183475 2308 2 0.17139110268964686 2308 3 0.13945599145921506 2308 4 0.2031017513939588 2308 5 0.18405156061927386 2308 6 0.1787345703214669 2308 7 0.19758376045916748 2308 8 0.17989723258171608 2308 9 0.19034487562711888 2308 10-11 0.2514969004294656 2308 12-13 0.20587090302380773 2308 14-15 0.37631008663778687 2308 16-17 0.383195528573836 2308 18-19 0.37432540559505867 2308 20-21 0.34798220496631416 2308 22-23 0.4013619039360421 2308 24-25 0.3942112220767555 2308 26-27 0.383812591795504 2308 28-29 0.417779286280485 2308 30-31 0.4443632791087424 2308 32-33 0.42143323788462084 2308 34-35 0.3994019776584352 2308 36-37 0.3989360540523066 2308 38-39 0.42114224644834763 2308 40-41 0.40667427447465343 2308 42-43 0.3521030369313749 2308 44-45 0.3793386396282372 2308 46-47 0.4218724441705888 2308 48-49 0.36713069502561524 2308 50-51 0.3162995234232113 2308 52-53 0.3676845794120567 2308 54-55 0.38590636170501824 2308 56-57 0.4153805790827505 2308 58-59 0.3814816218513144 2308 60-61 0.3298416855789057 2308 62-63 0.30060801326673925 2308 64-65 0.2816619917228138 2308 66-67 0.21464299642646267 2308 68-69 0.28128837004818763 2308 70-71 0.2654141762701485 2308 72-73 0.2091504564268547 2308 74-75 0.19992965470320456 2308 76-77 0.170706343340278 2308 78-79 0.3670229093983508 2308 80-81 0.4522866254898048 2308 82-83 0.43408538928274965 2308 84-85 0.45193031269464967 2308 86-87 0.4416932033233891 2308 88-89 0.38427156956122843 2308 90-91 0.5274204393112285 2308 92-93 0.5670718641667207 2309 1 0.2486556311142749 2309 2 0.20998210924048522 2309 3 0.16315519046918325 2309 4 0.22249489113184495 2309 5 0.21067671677386102 2309 6 0.19996440213925837 2309 7 0.2208709847098973 2309 8 0.23155897882019616 2309 9 0.24148738229136768 2309 10-11 0.25250526788057925 2309 12-13 0.2393684692776361 2309 14-15 0.36669277275417045 2309 16-17 0.3731708816383019 2309 18-19 0.38957750274455805 2309 20-21 0.32663479438917875 2309 22-23 0.3937515141769481 2309 24-25 0.3732347417440067 2309 26-27 0.4416012789261572 2309 28-29 0.4764208513027697 2309 30-31 0.4406390932851707 2309 32-33 0.4460594575116019 2309 34-35 0.4968236563520563 2309 36-37 0.46291873032935626 2309 38-39 0.5184528416947671 2309 40-41 0.46961321931301825 2309 42-43 0.4788327202618916 2309 44-45 0.46838200408156894 2309 46-47 0.5048640594713092 2309 48-49 0.5067066145680243 2309 50-51 0.5041733691880665 2309 52-53 0.4447267685528331 2309 54-55 0.48169590791095374 2309 56-57 0.5132301114990696 2309 58-59 0.5403271860686587 2309 60-61 0.5246283048879903 2309 62-63 0.4365812423528226 2309 64-65 0.5073757029910766 2309 66-67 0.5132931152213871 2309 68-69 0.5035216960539515 2309 70-71 0.4728861870471093 2309 72-73 0.4670706157613367 2309 74-75 0.4301743462861154 2309 76-77 0.379533255747333 2309 78-79 0.5253685916971946 2309 80-81 0.5805222993148291 2309 82-83 0.618815344153326 2309 84-85 0.5279177981112966 2309 86-87 0.555738969303647 2309 88-89 0.6600462966214025 2309 90-91 0.7142929196737242 2309 92-93 0.7140681615204372 2310 1 0.26380540296257493 2310 2 0.2278656536792738 2310 3 0.15568477938001024 2310 4 0.2154901472390236 2310 5 0.25363677298059173 2310 6 0.24683132237418448 2310 7 0.2473973719950635 2310 8 0.25563686556446186 2310 9 0.23658154769757544 2310 10-11 0.238740697104042 2310 12-13 0.23407118463182996 2310 14-15 0.4333284270797648 2310 16-17 0.43257429917216683 2310 18-19 0.37963877755590403 2310 20-21 0.3717576984211135 2310 22-23 0.4218409198387931 2310 24-25 0.4462325393454094 2310 26-27 0.44591275632151905 2310 28-29 0.48756347311923776 2310 30-31 0.5064707175955334 2310 32-33 0.4895152046111164 2310 34-35 0.5253118508598007 2310 36-37 0.5070101242987519 2310 38-39 0.5429411230489478 2310 40-41 0.5282224199947905 2310 42-43 0.4869097561579565 2310 44-45 0.508859882975834 2310 46-47 0.4985566472200773 2310 48-49 0.49960257983963885 2310 50-51 0.5001656181656031 2310 52-53 0.5172984522377106 2310 54-55 0.5277606314766601 2310 56-57 0.5842744936588673 2310 58-59 0.5899161483612971 2310 60-61 0.5834581053906049 2310 62-63 0.5242742664426459 2310 64-65 0.581879779030352 2310 66-67 0.5648550621226107 2310 68-69 0.5666860452998463 2310 70-71 0.564387600234916 2310 72-73 0.5435813885424778 2310 74-75 0.5483157808559582 2310 76-77 0.48065102152438044 2310 78-79 0.6259962734435263 2310 80-81 0.683606846202018 2310 82-83 0.628149976142808 2310 84-85 0.5903511972644466 2310 86-87 0.6386527483165381 2310 88-89 0.652911070864512 2310 90-91 0.7549392328822648 2310 92-93 0.7788791467741305 2311 1 0.21054416576983925 2311 2 0.17288562047624367 2311 3 0.12874365526416653 2311 4 0.18888550111927316 2311 5 0.1470719402641123 2311 6 0.16307178135428302 2311 7 0.17348046947414275 2311 8 0.17555236501366522 2311 9 0.1985623472764857 2311 10-11 0.2005038336672058 2311 12-13 0.18913974510524412 2311 14-15 0.3077824734455845 2311 16-17 0.29496877256789134 2311 18-19 0.26210537308682547 2311 20-21 0.17032912980216253 2311 22-23 0.23027017105412284 2311 24-25 0.27656638290497426 2311 26-27 0.3384502703202088 2311 28-29 0.3428530003567616 2311 30-31 0.328417334813885 2311 32-33 0.2817015671803418 2311 34-35 0.27004022455984256 2311 36-37 0.22950129196540558 2311 38-39 0.3150703827861676 2311 40-41 0.25968452617414073 2311 42-43 0.23368841869702095 2311 44-45 0.24705053075128802 2311 46-47 0.3304782657943477 2311 48-49 0.295582689736257 2311 50-51 0.3046632256419457 2311 52-53 0.24827623813801125 2311 54-55 0.27942175666978386 2311 56-57 0.3539925291524213 2311 58-59 0.3316163015656173 2311 60-61 0.2978717395833925 2311 62-63 0.2518407213006526 2311 64-65 0.3365282907432601 2311 66-67 0.2548295261118412 2311 68-69 0.2625797971326591 2311 70-71 0.2449850905678801 2311 72-73 0.20914756454340022 2311 74-75 0.2649132773796694 2311 76-77 0.2419446292852072 2311 78-79 0.3082138472815146 2311 80-81 0.37436669418470814 2311 82-83 0.34412533042306137 2311 84-85 0.365237056283398 2311 86-87 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-0.8952757782495624 2303 40-41 -1.0241937082184904 2303 42-43 -0.9632913735218125 2303 44-45 -0.9910732881355813 2303 46-47 -0.885224426605582 2303 48-49 -0.9046726293814302 2303 50-51 -0.9637196847265841 2303 52-53 -1.2609606473700765 2303 54-55 -1.60837183971298 2303 56-57 -1.8198923112841499 2303 58-59 -1.8264033615878432 2303 60-61 -2.016462018410202 2303 62-63 -2.048763643947325 2303 64-65 -2.072427219453175 2303 66-67 -2.086173703093422 2303 68-69 -2.141808195203623 2303 70-71 -2.054875867411319 2303 72-73 -1.8727147078137847 2303 74-75 -1.9264778079131375 2303 76-77 -1.830271923577648 2303 78-79 -2.0435028232840935 2303 80-81 -1.9560882873592718 2303 82-83 -1.8998828052057704 2303 84-85 -1.697842475153152 2303 86-87 -1.7943125087042233 2303 88-89 -2.1893721902005936 2303 90-91 -2.014524274720486 2303 92-93 -2.146895639116501 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 8.0 7 63.0 8 418.0 9 3558.0 10 20630.0 11 112164.0 12 80682.0 13 42438.0 14 20309.0 15 14470.0 16 18664.0 17 28688.0 18 46014.0 19 70665.0 20 104277.0 21 152368.0 22 222515.0 23 272937.0 24 344053.0 25 437624.0 26 552913.0 27 694439.0 28 873510.0 29 1105678.0 30 1412420.0 31 1830728.0 32 2429683.0 33 3346698.0 34 4857644.0 35 7689347.0 36 1.3843665E7 37 2.8281786E7 38 3.1982603E7 39 665091.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.66981868429845 24.04513315298955 11.454348368412306 24.830699794299697 2 15.555995913695275 22.735929695865696 38.31663642965279 23.39143796078624 3 15.262904476472977 27.336666707693823 31.67959235417923 25.720836461653967 4 11.452619296712493 17.41536696739572 41.88857976294509 29.243433972946693 5 11.689954829101382 38.537869952121305 38.186222260514235 11.585952958263073 6 28.50533213534038 42.08512708161532 18.199598754415547 11.209942028628749 7 25.95983999409209 32.70480190037786 26.185180498972272 15.15017760655778 8 23.173546346326635 37.69499427671176 22.809068642534495 16.322390734427117 9 23.579788053712754 14.984948121161395 24.85374327667483 36.58152054845102 10-11 22.56550321858038 27.106792373872267 31.53998547638682 18.787718931160533 12-13 21.942067030167266 25.368332123032232 31.61171095548143 21.077889891319064 14-15 19.91769788422957 26.94094009624971 27.093986485654852 26.047375533865868 16-17 20.032791856930103 31.513882851059115 28.252222482060873 20.201102809949905 18-19 20.122390242101247 30.214774207047647 29.813090452570556 19.849745098280554 20-21 20.408406548481146 29.752689218913837 29.607412871399607 20.23149136120541 22-23 20.523368850684818 29.56233504055794 29.539685937924748 20.374610170832497 24-25 20.075637709037988 29.486444719554267 29.83249108437491 20.605426487032826 26-27 20.330603485064753 30.027438325661336 29.54484843349156 20.097109755782352 28-29 20.31985881671272 29.84916300833768 29.58732476658706 20.243653408362537 30-31 20.157213027747623 29.7079257824171 29.741958705854298 20.39290248398098 32-33 20.400846113162416 29.94274933587335 29.568862823966974 20.087541726997262 34-35 20.254976493215533 29.652163115770346 29.710200304772012 20.38266008624211 36-37 20.181497321303027 29.85069878364565 29.751883816752166 20.215920078299156 38-39 20.245498660644046 29.8364928399392 29.737466247520988 20.180542251895766 40-41 20.1404819716225 29.745832127096524 29.89366820799198 20.220017693289 42-43 20.144965749957425 29.78012220895377 29.859207836629675 20.215704204459133 44-45 20.13979238919677 29.650069348869806 29.84158119096018 20.36855707097324 46-47 20.029164770771125 29.678687960775672 30.016505052513903 20.275642215939296 48-49 20.088450114536148 29.841266391996857 29.875659890575147 20.194623602891852 50-51 19.995411601844808 29.793516554112 29.99658580288753 20.214486041155663 52-53 20.041388294927405 29.65891036445064 30.09275413494375 20.206947205678205 54-55 20.02691370038883 29.765166076465565 29.98965579789155 20.218264425254056 56-57 19.958356275881037 29.781153456039654 29.897762712521974 20.362727555557335 58-59 19.988011814260283 29.7684613655336 29.877569644672374 20.365957175533747 60-61 19.893177940766225 29.70546032992437 30.014549190647433 20.386812538661975 62-63 19.862591507261065 29.831905324062785 29.90831231003223 20.397190858643917 64-65 19.92900969969004 29.692648312510737 29.931059601232725 20.44728238656649 66-67 19.898801549791408 29.628674917096188 29.899021249760278 20.573502283352123 68-69 19.90090455465798 29.809536584072145 29.78082709404265 20.50873176722723 70-71 19.964691742078717 29.708602182070003 29.785003412738153 20.541702663113124 72-73 19.919744326800704 29.758530949850403 29.7366931901259 20.585031533222992 74-75 19.865101574059217 29.79408367205348 29.76851120294071 20.572303550946593 76-77 19.92058233874289 29.751554298685516 29.773892880348445 20.553970482223143 78-79 20.01356066150616 29.76446593944252 29.68705727472424 20.534916124327083 80-81 19.891212002685705 29.83655374889943 29.637444437142513 20.634789811272352 82-83 19.962661474822525 29.807948154956627 29.691188154756375 20.538202215464473 84-85 19.87609534084661 29.7836255553608 29.697295016166475 20.64298408762611 86-87 19.804498573759073 29.694849704052544 29.78522654186071 20.715425180327674 88-89 19.906181376969215 29.705422002114908 29.726920105062067 20.661476515853813 90-91 19.98994275548421 29.748894980636585 29.662468578649055 20.598693685230156 92-93 19.949705770295843 29.6270999697047 29.66407264894263 20.759121611056823 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1504.0 1 887.0 2 384.0 3 825.5 4 1943.5 5 4341.5 6 8598.5 7 14572.5 8 21942.5 9 29458.5 10 36213.0 11 41473.0 12 46572.0 13 53419.5 14 57144.0 15 62960.0 16 79282.5 17 106218.0 18 148760.0 19 213171.0 20 305904.0 21 432880.0 22 601666.5 23 817232.0 24 1073805.0 25 1373026.0 26 1726248.0 27 2121586.0 28 2533470.0 29 2741270.0 30 2934044.0 31 3307113.0 32 3644232.0 33 3934210.5 34 4177280.5 35 4373820.0 36 4484653.0 37 4506861.0 38 4468425.0 39 4372801.5 40 4242887.5 41 4070171.0 42 3869979.0 43 3771778.0 44 3664084.0 45 3407350.5 46 3137433.0 47 2881082.0 48 2627780.0 49 2387165.0 50 2149367.5 51 1937876.0 52 1739846.5 53 1551217.5 54 1384351.0 55 1235600.5 56 1092922.0 57 1019207.0 58 963999.0 59 858615.0 60 761797.0 61 672754.5 62 592545.0 63 521490.0 64 459843.5 65 414360.0 66 380641.5 67 340053.5 68 291071.5 69 249369.5 70 213988.5 71 195280.0 72 183529.5 73 159599.5 74 138451.0 75 123450.5 76 114964.5 77 111475.5 78 104467.5 79 95720.0 80 85603.0 81 75665.0 82 67211.0 83 54320.0 84 36731.5 85 21058.0 86 15941.0 87 12433.5 88 6685.0 89 3308.0 90 1596.5 91 835.5 92 467.5 93 216.0 94 97.5 95 38.5 96 22.0 97 17.0 98 25.5 99 51.5 100 71.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.145355274656295 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0015877509323421173 22-23 6.326387435843785E-4 24-25 0.023577978263812818 26-27 0.13339027902568712 28-29 0.18187650005538666 30-31 0.23698942730193115 32-33 0.258343569608724 34-35 0.27288539884549584 36-37 0.2812460767782195 38-39 0.2810668701613598 40-41 0.280387952785949 42-43 0.2687739855010031 44-45 0.27097222051275743 46-47 0.26562556155919603 48-49 0.2679882334116952 50-51 0.26867010474232894 52-53 0.35004270926926534 54-55 0.4592130170960158 56-57 0.5103597671298633 58-59 0.5301955764520536 60-61 0.5561470577375165 62-63 0.5682878137038906 64-65 0.5718163132177188 66-67 0.5712245375213855 68-69 0.5641842775733258 70-71 0.561442022474676 72-73 0.564320159513582 74-75 0.560546481716248 76-77 0.5742740039632233 78-79 0.5441338141716002 80-81 0.5120484448656566 82-83 0.476890469801962 84-85 0.44734648663950666 86-87 0.43752704715251023 88-89 0.41773357785518234 90-91 0.4005459893903775 92-93 0.3754851256046377 >>END_MODULE >>Sequence Length Distribution pass #Length Count 93 1.0155875E8 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.04853897673452 #Duplication Level Percentage of deduplicated Percentage of total 1 83.5909956516969 53.53881143101754 2 11.257573213460436 14.420622334915265 3 2.32431973759278 4.466078499228137 4 0.8667847574327675 2.2206518928348795 5 0.4207480984386754 1.3474150491118215 6 0.2889510805548106 1.1104136727170606 7 0.14471315963676484 0.6488066511809215 8 0.1266290166678743 0.648832280771033 9 0.11039888817329639 0.6363798742939977 >10 0.7322749801814933 9.102009989851064 >50 0.0799099872194917 3.5149910877170636 >100 0.05355533719101515 6.431254405178407 >500 0.0023991677598238386 1.025279458117728 >1k 7.412799648001077E-4 0.8070034965485853 >5k 0.0 0.0 >10k+ 5.644029097240989E-6 0.08144987651661 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 9.846517409873596E-7 9.846517409873596E-7 2 1.969303481974719E-6 1.969303481974719E-6 3.938606963949438E-6 3 2.9539552229620787E-6 3.938606963949438E-6 8.861865668886236E-6 4 3.938606963949438E-6 7.877213927898876E-6 1.5754427855797753E-5 5 1.3785124373823033E-5 8.861865668886236E-6 1.6739079596785113E-5 6 1.6739079596785113E-5 1.2800472632835675E-5 1.6739079596785113E-5 7 2.067768656073455E-5 1.2800472632835675E-5 1.969303481974719E-5 8 2.8554900488633426E-5 1.4769776114810393E-5 2.166233830172191E-5 9 3.840141789850702E-5 1.8708383078759833E-5 4.234002486245646E-5 10-11 5.5632823365785815E-5 2.313931591320295E-5 8.123376863145717E-5 12-13 6.498701490516572E-5 2.8554900488633426E-5 9.944982583972331E-5 14-15 7.877213927898877E-5 3.5447462675544946E-5 1.2357379349391362E-4 16-17 9.403424126429283E-5 4.332467660344382E-5 1.6443684074488903E-4 18-19 1.142196019545337E-4 4.677095769689958E-5 2.2548524868610533E-4 20-21 1.2997402981033144E-4 5.021723879035534E-5 2.9539552229620786E-4 22-23 1.4277450244316712E-4 5.2678868142823735E-5 4.1010745012123526E-4 24-25 1.5754427855797751E-4 5.612514923627949E-5 8.477851489901166E-4 26-27 1.683754477088385E-4 5.7602126847760535E-5 0.0023454404470318905 28-29 1.8609917904661093E-4 6.104840794121629E-5 0.011178258889559 30-31 2.1219245018277598E-4 6.498701490516573E-5 0.021354142306792865 32-33 2.284392039090674E-4 6.892562186911516E-5 0.03382820288749123 34-35 2.4025502480091573E-4 7.237190296257093E-5 0.056637660467463415 36-37 2.535478233042451E-4 7.483353231503933E-5 0.10752150848646719 38-39 2.678252735485618E-4 7.975679101997611E-5 0.19547355594668112 40-41 2.850566790158406E-4 8.418772385441925E-5 0.29728162270606917 42-43 2.9982645513065094E-4 8.615702733639395E-5 0.4036840744889042 44-45 3.131192536339803E-4 9.108028604133076E-5 0.5312486614890396 46-47 3.229657710438539E-4 9.304958952330547E-5 0.6860103142269869 48-49 3.38227873029158E-4 9.944982583972331E-5 0.8423636565042402 50-51 3.5447462675544943E-4 1.0683471389712852E-4 0.9992925277241006 52-53 3.726906839637156E-4 1.1372727608404003E-4 1.1531187613081098 54-55 3.918913929129691E-4 1.1914286065947051E-4 1.3157463044789346 56-57 4.0075325858185537E-4 1.2406611936440732E-4 1.497994510566544 58-59 4.27338855588514E-4 1.3342031090378723E-4 1.6848110084064642 60-61 4.928181963641735E-4 1.3686659199724298E-4 1.867223159008948 62-63 5.090649500904649E-4 1.3982054722020505E-4 2.042089923320246 64-65 5.307272883921868E-4 1.462207835366229E-4 2.2160951173581793 66-67 5.435277610250225E-4 1.5606730094649648E-4 2.4053855526973305 68-69 5.651900993267443E-4 1.6148288552192695E-4 2.602327224389824 70-71 5.799598754415548E-4 1.673907959678511E-4 2.794987630312504 72-73 5.942373256858715E-4 1.708370770613069E-4 2.9821984811746898 74-75 6.124533828941376E-4 1.7871429098920578E-4 3.1636786589043284 76-77 6.247615296564796E-4 1.8462220143512992E-4 3.3534436963826355 78-79 6.375620022893153E-4 1.9102243775154774E-4 3.5523019926889607 80-81 6.474085196991889E-4 2.0037662929092766E-4 3.752428520437678 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACTG 43255 0.0 16.575369 5 CTATACT 51150 0.0 14.627131 4 TATACAG 42530 0.0 14.0142975 5 TACACTG 31360 0.0 13.725786 5 CTTACAC 31875 0.0 13.218437 3 TACACCG 17725 0.0 13.206008 5 GTGTAGG 32460 0.0 13.132761 1 GTATATA 85710 0.0 13.077292 1 GTATTAG 44455 0.0 12.948398 1 ACTGTGC 27830 0.0 12.943504 8 TATACCG 17095 0.0 12.906625 5 TCTATAC 52395 0.0 12.757294 3 GTATAAG 45860 0.0 12.731551 1 GTATAGG 36470 0.0 12.652915 1 TTATACT 75425 0.0 12.626378 4 GTATAGA 50670 0.0 12.3454275 1 ACTGCGC 10780 0.0 12.264365 8 TAGACTG 29895 0.0 12.208929 5 TAATACT 65465 0.0 12.130546 4 CCTATAC 37625 0.0 12.004808 3 >>END_MODULE