##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename lane7_Undetermined_L007_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 155408834 Sequences flagged as poor quality 0 Sequence length 93 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.023955311317756 33.0 33.0 33.0 33.0 33.0 2 32.00793960013882 33.0 33.0 33.0 33.0 33.0 3 32.0052771967905 33.0 33.0 33.0 33.0 33.0 4 35.78863834728983 37.0 37.0 37.0 33.0 37.0 5 35.790793070360465 37.0 37.0 37.0 33.0 37.0 6 35.865893350695885 37.0 37.0 37.0 33.0 37.0 7 35.760800792057935 37.0 37.0 37.0 33.0 37.0 8 35.77798267889971 37.0 37.0 37.0 33.0 37.0 9 35.81141943320931 37.0 37.0 37.0 33.0 37.0 10-11 35.81119320411348 37.0 37.0 37.0 33.0 37.0 12-13 35.81571889600562 37.0 37.0 37.0 33.0 37.0 14-15 37.754041781176994 40.0 37.0 40.0 33.0 40.0 16-17 37.68793135337467 40.0 37.0 40.0 33.0 40.0 18-19 37.73074446655973 40.0 37.0 40.0 33.0 40.0 20-21 37.693740620948226 40.0 37.0 40.0 33.0 40.0 22-23 37.60879992188861 40.0 37.0 40.0 33.0 40.0 24-25 37.529538562138626 40.0 37.0 40.0 33.0 40.0 26-27 37.45127154740766 40.0 37.0 40.0 33.0 40.0 28-29 37.36422144445147 40.0 37.0 40.0 33.0 40.0 30-31 37.267098300859786 40.0 37.0 40.0 33.0 40.0 32-33 37.1825039720715 40.0 37.0 40.0 33.0 40.0 34-35 37.08386522930865 40.0 37.0 40.0 33.0 40.0 36-37 36.99965133256195 40.0 37.0 40.0 33.0 40.0 38-39 36.87373395067104 40.0 37.0 40.0 33.0 40.0 40-41 36.798954118657115 40.0 37.0 40.0 33.0 40.0 42-43 36.795683172038984 40.0 37.0 40.0 33.0 40.0 44-45 36.699920327566446 40.0 37.0 40.0 33.0 40.0 46-47 36.56718955886382 40.0 37.0 40.0 33.0 40.0 48-49 36.40496476860511 40.0 37.0 40.0 33.0 40.0 50-51 35.34187779505507 37.0 35.0 38.5 30.0 40.0 52-53 35.45273263230325 37.0 35.0 38.5 27.0 40.0 54-55 36.102714457017285 37.0 37.0 40.0 27.0 40.0 56-57 36.116770150916906 37.0 37.0 40.0 27.0 40.0 58-59 35.977166079246174 37.0 37.0 40.0 27.0 40.0 60-61 36.118659017157285 37.0 37.0 40.0 27.0 40.0 62-63 36.03443396917835 37.0 37.0 40.0 27.0 40.0 64-65 35.85380256118516 37.0 37.0 40.0 27.0 40.0 66-67 35.665158288234764 37.0 37.0 40.0 27.0 40.0 68-69 35.43699300903319 37.0 35.0 40.0 27.0 40.0 70-71 35.22768651298162 37.0 33.0 40.0 27.0 40.0 72-73 34.999789053175704 37.0 33.0 38.5 27.0 40.0 74-75 34.72934440779602 37.0 33.0 37.0 27.0 40.0 76-77 34.48802479915653 37.0 33.0 37.0 27.0 40.0 78-79 34.205026903425576 37.0 33.0 37.0 27.0 40.0 80-81 33.95558469024998 37.0 33.0 37.0 27.0 37.0 82-83 33.674546818233 37.0 33.0 37.0 27.0 37.0 84-85 33.47399010470666 37.0 33.0 37.0 27.0 37.0 86-87 33.31556354125918 37.0 33.0 37.0 27.0 37.0 88-89 33.163303467678034 37.0 33.0 37.0 27.0 37.0 90-91 32.94088523629229 37.0 33.0 37.0 24.5 37.0 92-93 32.03345386401908 35.0 30.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2304 1 0.2508842555582049 2304 2 0.2787696791668708 2304 3 0.3018222620676738 2304 4 0.009095621509864316 2304 5 0.22557987509436828 2304 6 0.29711430940440664 2304 7 0.4205370721039259 2304 8 0.4713273759869665 2304 9 0.5005496284574704 2304 10-11 0.6082527165389493 2304 12-13 0.6047958429210709 2304 14-15 1.0102960060894617 2304 16-17 1.088332481519565 2304 18-19 1.053394624506005 2304 20-21 0.9880297522151622 2304 22-23 1.0274636770713315 2304 24-25 1.0457380083637702 2304 26-27 1.0361870529386152 2304 28-29 1.0601101187184483 2304 30-31 1.055586370103704 2304 32-33 1.0550461153277482 2304 34-35 1.097634729161065 2304 36-37 1.0680233202605578 2304 38-39 1.0332186428121375 2304 40-41 1.0762225815805024 2304 42-43 1.0690224758243616 2304 44-45 1.1084328785625104 2304 46-47 1.1031206979024901 2304 48-49 1.1012577243887591 2304 50-51 1.0302015000252709 2304 52-53 1.042393792257883 2304 54-55 1.1141375298471843 2304 56-57 1.054030588642938 2304 58-59 1.0417064159451144 2304 60-61 1.0854803424751083 2304 62-63 1.0995732821865332 2304 64-65 1.0827094861657898 2304 66-67 1.05594436423781 2304 68-69 1.0297233238474917 2304 70-71 1.047530482846419 2304 72-73 1.042604963693769 2304 74-75 1.079503359217874 2304 76-77 1.1066850369277716 2304 78-79 1.0979942147630979 2304 80-81 1.1540054676024383 2304 82-83 1.1723181129783669 2304 84-85 1.2084491420096484 2304 86-87 1.1490553686088703 2304 88-89 1.136521523252874 2304 90-91 1.2466671020230606 2304 92-93 1.2773895184570705 2305 1 0.3383098371505753 2305 2 0.3330288179257721 2305 3 0.37337908619002036 2305 4 0.265386585351159 2305 5 0.40772646542148294 2305 6 0.40284070997302734 2305 7 0.4941390831120316 2305 8 0.5310649163978951 2305 9 0.4294076170357286 2305 10-11 0.5634720901655257 2305 12-13 0.5955072116951072 2305 14-15 1.0476826347524337 2305 16-17 1.100402345416164 2305 18-19 1.0848345042017016 2305 20-21 1.0356878008702495 2305 22-23 1.090721835749001 2305 24-25 1.108681497885044 2305 26-27 1.095778683471039 2305 28-29 1.1007227103390207 2305 30-31 1.1061073953951137 2305 32-33 1.1077070922853451 2305 34-35 1.1023581084817238 2305 36-37 1.1068218452564267 2305 38-39 1.1195260440911952 2305 40-41 1.14904327852053 2305 42-43 1.1375739650676735 2305 44-45 1.1500026745485528 2305 46-47 1.1143946252516557 2305 48-49 1.1009748507394406 2305 50-51 1.0660759621114835 2305 52-53 1.0415933751317397 2305 54-55 1.1287064830519284 2305 56-57 1.1219774937008324 2305 58-59 1.1105485678114206 2305 60-61 1.1083352461950042 2305 62-63 1.0810916244765636 2305 64-65 1.109747275967429 2305 66-67 1.0866673486132612 2305 68-69 1.1408396913808403 2305 70-71 1.1075587368612005 2305 72-73 1.089183798161649 2305 74-75 1.111932777095177 2305 76-77 1.0953805378945418 2305 78-79 1.1437113240365093 2305 80-81 1.1712285519653918 2305 82-83 1.1909846589208328 2305 84-85 1.1867138890530882 2305 86-87 1.1714061168794245 2305 88-89 1.1406739963308468 2305 90-91 1.195959745326725 2305 92-93 1.2170422109312113 2306 1 0.3308111287022726 2306 2 0.3353324009546981 2306 3 0.37316219573132514 2306 4 0.20803992778557046 2306 5 0.33468603682683096 2306 6 0.42003401532983276 2306 7 0.5343615312626824 2306 8 0.5320999619909799 2306 9 0.5458123582193437 2306 10-11 0.6083715197364725 2306 12-13 0.6025815699571027 2306 14-15 1.0170188718225717 2306 16-17 1.0093816893401453 2306 18-19 0.9929069996470901 2306 20-21 0.9546579369731205 2306 22-23 0.9979728067150546 2306 24-25 0.9600825860215991 2306 26-27 0.953722067940717 2306 28-29 1.0081448259548225 2306 30-31 1.007364310619053 2306 32-33 1.0022598344330689 2306 34-35 1.010831198841295 2306 36-37 1.028612237733661 2306 38-39 1.0085678856477998 2306 40-41 1.0164194362810974 2306 42-43 1.0278999031553226 2306 44-45 1.0209004606047216 2306 46-47 0.9987928132798345 2306 48-49 1.0311298118169745 2306 50-51 0.9495319560058988 2306 52-53 0.9631673601442472 2306 54-55 1.0545453461312917 2306 56-57 1.022918794696487 2306 58-59 1.025609876467641 2306 60-61 1.043575925318315 2306 62-63 0.9836486537048685 2306 64-65 1.0246654272904792 2306 66-67 1.008523891717843 2306 68-69 0.9904722351354636 2306 70-71 0.9797086053557393 2306 72-73 0.9969243395278227 2306 74-75 0.9984092449626729 2306 76-77 1.01146343537269 2306 78-79 1.017564770626727 2306 80-81 0.9621924309317009 2306 82-83 1.057839393050287 2306 84-85 1.0742350792396351 2306 86-87 1.0825724193545767 2306 88-89 1.0214091226572322 2306 90-91 1.11285318043916 2306 92-93 1.1012041769658651 2307 1 0.2509952439446792 2307 2 0.26152630489567485 2307 3 0.29521420219364103 2307 4 0.19541938092820033 2307 5 0.2867940565435134 2307 6 0.31556865942803825 2307 7 0.40465533884244564 2307 8 0.44897154161483854 2307 9 0.43928276995623605 2307 10-11 0.514225131108681 2307 12-13 0.5239962900060959 2307 14-15 0.8895138510163463 2307 16-17 0.9419331531893675 2307 18-19 0.8952629568000461 2307 20-21 0.8761645398752975 2307 22-23 0.9055227112856059 2307 24-25 0.9044658875651947 2307 26-27 0.8771689805932112 2307 28-29 0.8873070461227499 2307 30-31 0.9102087744935048 2307 32-33 0.9003341183768399 2307 34-35 0.875933114482784 2307 36-37 0.8998149362405243 2307 38-39 0.8962240217809381 2307 40-41 0.9425066089877348 2307 42-43 0.9380598691712905 2307 44-45 0.9117853156390154 2307 46-47 0.883946683534532 2307 48-49 0.9168504130594428 2307 50-51 0.796847663737168 2307 52-53 0.8289970064258227 2307 54-55 0.8917140139468245 2307 56-57 0.9342627784879127 2307 58-59 0.8938509680160678 2307 60-61 0.9073808261882519 2307 62-63 0.9282135546510872 2307 64-65 0.9418864530364672 2307 66-67 0.9354135388167606 2307 68-69 0.9518291862203583 2307 70-71 0.9271153005625266 2307 72-73 0.9242136539845234 2307 74-75 0.9388733977462067 2307 76-77 0.932859408321093 2307 78-79 0.9230839254022527 2307 80-81 0.9040207194008545 2307 82-83 0.957539386537988 2307 84-85 0.906541193925527 2307 86-87 0.9062874261050808 2307 88-89 0.8728956195841633 2307 90-91 0.9527577936942251 2307 92-93 0.9810336218230162 2308 1 0.36306478903595263 2308 2 0.3853058632950592 2308 3 0.40388056931560357 2308 4 0.401794662465079 2308 5 0.4450937267843784 2308 6 0.43596624836047226 2308 7 0.5288894968791524 2308 8 0.5704409700171524 2308 9 0.5315186039803805 2308 10-11 0.5783339742471796 2308 12-13 0.5765722637090889 2308 14-15 0.9471950488204683 2308 16-17 0.9715917498567919 2308 18-19 0.9277636295357183 2308 20-21 0.9159107489128075 2308 22-23 0.9507656603585488 2308 24-25 0.9592139914320867 2308 26-27 0.9760946488247413 2308 28-29 0.9606533082028719 2308 30-31 0.9919753329718048 2308 32-33 0.9970908147973603 2308 34-35 0.9670527475289106 2308 36-37 0.9380057834729882 2308 38-39 0.9711221955725975 2308 40-41 0.9773146753495965 2308 42-43 0.9783634096226024 2308 44-45 0.9819149027662633 2308 46-47 0.9786034152238798 2308 48-49 0.9462708334525942 2308 50-51 0.890787131535248 2308 52-53 0.8703664864136158 2308 54-55 0.962591975717956 2308 56-57 0.9304551945973358 2308 58-59 0.9293094024017208 2308 60-61 0.927280370353408 2308 62-63 0.9434233511516297 2308 64-65 0.9339218190450751 2308 66-67 0.9216288445318526 2308 68-69 0.9060006576860005 2308 70-71 0.8170360660978773 2308 72-73 0.8969156923070614 2308 74-75 0.8930898597857322 2308 76-77 0.8785498883120084 2308 78-79 0.8874100379516534 2308 80-81 0.9013904418251073 2308 82-83 0.9172880468220086 2308 84-85 0.9496421793373528 2308 86-87 0.9645234677131072 2308 88-89 0.9191761377190772 2308 90-91 0.9558371408593231 2308 92-93 0.9330497890054872 2309 1 0.3131117025865251 2309 2 0.3342098568935725 2309 3 0.3554388349631239 2309 4 0.400138672314462 2309 5 0.3982181320840539 2309 6 0.4060962537055772 2309 7 0.4905764487596187 2309 8 0.5146153468270427 2309 9 0.5277996844246502 2309 10-11 0.5535498383517776 2309 12-13 0.5309696633528489 2309 14-15 0.8548970755272478 2309 16-17 0.9000954020264302 2309 18-19 0.8621980225193866 2309 20-21 0.8547506225612054 2309 22-23 0.8831707901249573 2309 24-25 0.8390825381477782 2309 26-27 0.8337446599905292 2309 28-29 0.8452074072979556 2309 30-31 0.8265448806916353 2309 32-33 0.8333475367910168 2309 34-35 0.7901623720636053 2309 36-37 0.8167540675798435 2309 38-39 0.814765356725168 2309 40-41 0.8269838315512459 2309 42-43 0.8371767552916438 2309 44-45 0.8278914871640737 2309 46-47 0.8246048678462472 2309 48-49 0.8076665710022723 2309 50-51 0.7289231952834285 2309 52-53 0.7402357792152046 2309 54-55 0.8764427557914871 2309 56-57 0.8065078437686353 2309 58-59 0.8037371616216404 2309 60-61 0.8060969628770494 2309 62-63 0.7750840933417678 2309 64-65 0.7901630686986962 2309 66-67 0.8182125932999966 2309 68-69 0.7981629164695434 2309 70-71 0.7806640243311378 2309 72-73 0.8147018550922525 2309 74-75 0.8209208383929507 2309 76-77 0.8546053028504303 2309 78-79 0.867294095016824 2309 80-81 0.8380655043158001 2309 82-83 0.8597899051229803 2309 84-85 0.8190000151585721 2309 86-87 0.826879219900178 2309 88-89 0.8088976604655471 2309 90-91 0.9066937209373833 2309 92-93 0.9052555655645733 2310 1 0.3268360470111915 2310 2 0.326295579712486 2310 3 0.3597710993478884 2310 4 0.43485075969278597 2310 5 0.41721018561835876 2310 6 0.4008131471616423 2310 7 0.47657029562257236 2310 8 0.48397286077612733 2310 9 0.4698513364243979 2310 10-11 0.4836085390248499 2310 12-13 0.44998268927582075 2310 14-15 0.7289865131354674 2310 16-17 0.7544753560122146 2310 18-19 0.7175783305959769 2310 20-21 0.730273153669529 2310 22-23 0.7677231926802861 2310 24-25 0.7313758101914374 2310 26-27 0.7118655076532008 2310 28-29 0.737471489029204 2310 30-31 0.7199355590956529 2310 32-33 0.7328819817814818 2310 34-35 0.7377788391945188 2310 36-37 0.7127233401728645 2310 38-39 0.7334172489255977 2310 40-41 0.710866532630412 2310 42-43 0.7257186890816314 2310 44-45 0.7356954790001211 2310 46-47 0.7184180685790977 2310 48-49 0.7485116178790747 2310 50-51 0.7252377411409014 2310 52-53 0.7311841007390854 2310 54-55 0.817041673470122 2310 56-57 0.7721147341656618 2310 58-59 0.7615967429948398 2310 60-61 0.7394494922068446 2310 62-63 0.7262125037151819 2310 64-65 0.7498181242357376 2310 66-67 0.7589163767966554 2310 68-69 0.7735451321724369 2310 70-71 0.7565440319081063 2310 72-73 0.755800853433719 2310 74-75 0.7830615244252357 2310 76-77 0.7417572113975979 2310 78-79 0.8032154476593334 2310 80-81 0.7637931668510092 2310 82-83 0.8142578863771774 2310 84-85 0.7968211192020362 2310 86-87 0.7736884825478967 2310 88-89 0.7561793109379238 2310 90-91 0.8205428077399048 2310 92-93 0.830151463982375 2311 1 0.3161737823674642 2311 2 0.3332635388525347 2311 3 0.34371409321759216 2311 4 0.4089292163443474 2311 5 0.42318963305832114 2311 6 0.3971655430003622 2311 7 0.45076768750934093 2311 8 0.4199954964585757 2311 9 0.4615586587120788 2311 10-11 0.44074495595910435 2311 12-13 0.42740559604926176 2311 14-15 0.6366924646621896 2311 16-17 0.6217963590888687 2311 18-19 0.6253626896659839 2311 20-21 0.6184554424539144 2311 22-23 0.6501454886782554 2311 24-25 0.6438587336847235 2311 26-27 0.6067413439973421 2311 28-29 0.6177069342826655 2311 30-31 0.5685894272705028 2311 32-33 0.5854714668649805 2311 34-35 0.5923254532247881 2311 36-37 0.6140590872083536 2311 38-39 0.5900369905504945 2311 40-41 0.6214856388730894 2311 42-43 0.6177152328439277 2311 44-45 0.5823565804649533 2311 46-47 0.6164603281602226 2311 48-49 0.52058045928311 2311 50-51 0.45484632213303655 2311 52-53 0.5462429483117432 2311 54-55 0.6242049068898297 2311 56-57 0.5731096816061623 2311 58-59 0.5525697118017803 2311 60-61 0.49462669534597836 2311 62-63 0.5123096678391832 2311 64-65 0.49935280149444594 2311 66-67 0.5762893201000452 2311 68-69 0.582274205630597 2311 70-71 0.6288529498306232 2311 72-73 0.534421738424733 2311 74-75 0.5018888960841466 2311 76-77 0.5466810431558855 2311 78-79 0.4737029576398655 2311 80-81 0.5405757103450171 2311 82-83 0.637246326034159 2311 84-85 0.6156460007577138 2311 86-87 0.5848133499412 2311 88-89 0.5803818869025932 2311 90-91 0.6131452195656166 2311 92-93 0.693011325271442 2312 1 0.15174243065365545 2312 2 0.13899838801421538 2312 3 0.10167583765842636 2312 4 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66-67 -1.1033754921418932 2303 68-69 -0.9349360691139239 2303 70-71 -0.9910148689786169 2303 72-73 -1.0239435162802693 2303 74-75 -1.2069980597694894 2303 76-77 -1.2280722516744191 2303 78-79 -1.2141369088628338 2303 80-81 -1.0925737603841768 2303 82-83 -0.924020477217411 2303 84-85 -0.9110655975174708 2303 86-87 -1.0525726897382839 2303 88-89 -1.0668625605967748 2303 90-91 -1.2558062346417103 2303 92-93 -1.1589759943420361 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 399632.0 3 24486.0 4 15106.0 5 12330.0 6 10735.0 7 24552.0 8 48076.0 9 53826.0 10 69314.0 11 112855.0 12 179486.0 13 241373.0 14 284601.0 15 317695.0 16 349797.0 17 386516.0 18 425497.0 19 471532.0 20 523405.0 21 585138.0 22 655972.0 23 742672.0 24 844869.0 25 967400.0 26 1124810.0 27 1315973.0 28 1559292.0 29 1871348.0 30 2307694.0 31 2932608.0 32 3830589.0 33 5177810.0 34 7319728.0 35 1.111807E7 36 1.895194E7 37 3.7325132E7 38 5.0294081E7 39 2532894.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.93917162289894 23.23996022911933 11.026215225951026 24.794652922030703 2 15.860610787463415 22.02546634086219 38.44336237418553 23.670560497488864 3 15.54538362257569 26.456357232878336 31.667382790375992 26.33087635416998 4 11.636250243678708 16.675373714110595 42.066188725749484 29.62218731646122 5 12.25661103392724 38.0889006553435 37.68926379915421 11.965224511575045 6 29.093928188910112 41.40617667475892 18.0373483807114 11.462546755619567 7 26.77791439062397 32.73569509892692 25.41629983096097 15.070090679488143 8 23.778631875983507 37.194305470996206 22.550097583841843 16.476965069178444 9 24.012434856242553 14.967568254860195 24.777978531454274 36.24201835744298 10-11 22.849387412971115 26.747210670684463 31.341008396058673 19.062393520285756 12-13 22.179081275070367 25.293355552176543 31.313324981983857 21.214238190769226 14-15 20.349410714611047 26.656225830593765 26.854251564152214 26.14011189064297 16-17 20.46676413232685 31.044207495210742 27.955645521220674 20.53338285124174 18-19 20.37942019008857 29.920433573661764 29.681470199836845 20.01867603641282 20-21 20.79154883234125 29.62496363991283 29.213005647222296 20.370481880523624 22-23 20.88173758746126 29.20000802605735 29.25038612170512 20.667868264776278 24-25 20.31791714191142 29.004665966647845 29.776759804214276 20.900657087226456 26-27 20.73166839671095 29.816761639547757 29.221578847854257 20.229991115887035 28-29 20.811258343557892 29.606807211682796 29.153202307654208 20.4287321371051 30-31 20.481782391124135 29.299284756070726 29.451727770506686 20.76720508229845 32-33 20.592887124916828 29.65996987265578 29.416254956172637 20.330888046254756 34-35 20.524492046032165 29.46170809344665 29.4054848375957 20.60831502292549 36-37 20.544110132570967 29.518179619867364 29.425785041817555 20.51192520574412 38-39 20.55370905286823 29.481076314231945 29.53915230695456 20.426062325945267 40-41 20.407356733757823 29.508060459431274 29.663903933209124 20.42067887360178 42-43 20.516842257651955 29.55258334930845 29.549784860264 20.380789532775598 44-45 20.423780046719294 29.25374472308682 29.590436772231655 20.73203845796223 46-47 20.31272174336439 29.26718626458421 29.90498588977663 20.51510610227477 48-49 20.44259300195163 29.658055469441724 29.55423765346193 20.34511387514472 50-51 20.388955725735503 29.44888621149971 29.675033587459637 20.487124475305155 52-53 20.37398462186074 29.26530118001045 29.945680553313125 20.415033644815686 54-55 20.33123086739114 29.560677560680748 29.772323066321032 20.335768505607074 56-57 20.388867698416245 29.50644761458449 29.63524329546831 20.46944139153095 58-59 20.458709823394027 29.39438988111675 29.617025387680783 20.52987490780844 60-61 20.186384897456865 29.360424012101788 29.935642835305405 20.51754825513595 62-63 20.28804408889581 29.595254258828597 29.64515733722135 20.471544315054242 64-65 20.406315785674316 29.37259739092176 29.724181581138403 20.49690524226552 66-67 20.24602219730212 29.229211148667805 29.78980142178782 20.734965232242253 68-69 20.255936521544395 29.56163026011615 29.67568148829024 20.50675173004921 70-71 20.495234327050557 29.482503923855013 29.517323810323692 20.504937938770738 72-73 20.420948644353434 29.392444113304716 29.473927108529963 20.712680133811883 74-75 20.301458547150784 29.520887117937487 29.64756095418833 20.5300933807234 76-77 20.36643535277128 29.609214443004372 29.52666849491021 20.497681709314136 78-79 20.64267452518394 29.38446947161489 29.432164814385224 20.540691188815952 80-81 20.401609451969442 29.527732735652922 29.460445931550794 20.61021188082684 82-83 20.531455863196708 29.65733702725053 29.48461171302863 20.32659539652413 84-85 20.549092474812007 29.65824954411163 29.428620029699847 20.364037951376517 86-87 20.36523454064694 29.414012159169932 29.54984315733153 20.670910142851593 88-89 20.46904296862673 29.467410805401386 29.517649961559954 20.545896264411933 90-91 20.68408252619932 29.66618660647725 29.32069122225031 20.32903964507312 92-93 20.82590745321229 29.469413089772083 29.25535125223612 20.4493282047795 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 374985.0 1 196828.0 2 16828.0 3 14074.5 4 12535.0 5 11491.0 6 11041.5 7 11255.0 8 12183.0 9 13980.5 10 16690.0 11 20749.0 12 26767.5 13 36160.0 14 42001.0 15 50875.0 16 74139.5 17 112264.5 18 173442.5 19 267509.5 20 408636.0 21 605748.5 22 870811.5 23 1211557.5 24 1625272.0 25 2094697.5 26 2629074.0 27 3219185.0 28 3832717.5 29 4146512.0 30 4412059.5 31 4908272.5 32 5347690.0 33 5736973.5 34 6063988.0 35 6312973.5 36 6439705.5 37 6482087.5 38 6456688.5 39 6337594.5 40 6167546.5 41 5960032.5 42 5785391.0 43 5723758.0 44 5615876.0 45 5245382.0 46 4808654.5 47 4457596.0 48 4103135.0 49 3762632.5 50 3446220.5 51 3160396.0 52 2881346.0 53 2607082.5 54 2353622.0 55 2124326.5 56 1937037.5 57 1853194.0 58 1786110.5 59 1650895.5 60 1496469.5 61 1331015.5 62 1169086.0 63 1033003.5 64 919874.5 65 811215.0 66 713563.0 67 620321.0 68 532117.0 69 456833.0 70 380713.5 71 336107.0 72 305070.5 73 253813.0 74 216924.0 75 186790.0 76 170010.0 77 151252.0 78 123109.0 79 101308.0 80 84187.5 81 68159.0 82 54359.0 83 40528.5 84 26149.5 85 15632.0 86 11453.0 87 9652.0 88 6748.0 89 4899.0 90 3336.0 91 2112.0 92 1506.5 93 1111.5 94 817.0 95 605.0 96 426.0 97 307.0 98 222.5 99 181.0 100 170.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.46768126450263436 2 0.45891213623029947 3 0.4540166616268416 4 0.4583439574612599 5 0.45388989920611594 6 0.32976246382493285 7 0.32949349584593113 8 0.33095802005695507 9 0.3294465229692155 10-11 0.328105222126562 12-13 0.31000586491756316 14-15 0.29406500791325674 16-17 0.29350937669347676 18-19 0.28669316185719534 20-21 0.2940576080765138 22-23 0.29825653283004494 24-25 0.31048621084178524 26-27 0.3291617901206311 28-29 0.3264228853296718 30-31 0.32645795412119233 32-33 0.319206757577243 34-35 0.3203472976317421 36-37 0.3143244739871094 38-39 0.32100427444169616 40-41 0.317730329281024 42-43 0.32418652597316316 44-45 0.31995993226485436 46-47 0.2990788799046005 48-49 0.29320147913856687 50-51 0.297194817123459 52-53 0.3210467430699596 54-55 0.3387835082785577 56-57 0.34390805608901226 58-59 0.3538328458213643 60-61 0.355209858919603 62-63 0.3478775859035143 64-65 0.3445122688456694 66-67 0.3337677058950201 68-69 0.33889901007815293 70-71 0.33666393764977354 72-73 0.3153022176332653 74-75 0.32399895619833297 76-77 0.3174179274776619 78-79 0.3470944901368992 80-81 0.36448217609045314 82-83 0.36616451288734336 84-85 0.36373768816771385 86-87 0.3521524394166679 88-89 0.341444875649733 90-91 0.3177969278117099 92-93 0.3153282779278815 >>END_MODULE >>Sequence Length Distribution pass #Length Count 93 1.55408834E8 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.297327435752415 #Duplication Level Percentage of deduplicated Percentage of total 1 76.86162274513786 39.427958292006124 2 14.220981720946757 14.589967115945113 3 3.9362306871911703 6.057543432705065 4 1.6064602851577525 3.296284770410777 5 0.7905202243984708 2.027578739777641 6 0.452661368177116 1.3932191052538319 7 0.2840754779378127 1.0200618965770685 8 0.2455411240390328 1.0076482751018372 9 0.17515047882861548 0.8086276316700277 >10 1.1848914713725818 11.910363018658716 >50 0.13527874436907505 4.834835548342729 >100 0.09837049890150054 9.421078627507292 >500 0.00606099445410863 2.0122666961131914 >1k 0.0021068244730046383 1.6969405509838997 >5k 3.8618223830673406E-5 0.13366807517914295 >10k+ 8.7363911835236E-6 0.3619582237674343 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAAC 162356 0.10447025167179365 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 1.930392193792536E-6 0.0 2 6.434640645975119E-7 2.5738562583900476E-6 0.0 3 6.434640645975119E-7 3.860784387585072E-6 5.147712516780095E-6 4 1.930392193792536E-6 3.860784387585072E-6 1.1582353162755213E-5 5 1.2225817227352726E-5 6.434640645975119E-6 1.2225817227352726E-5 6 1.6086601614937797E-5 8.365032839767654E-6 1.2869281291950238E-5 7 1.9947386002522867E-5 9.651960968962678E-6 1.351274535654775E-5 8 3.2173203229875594E-5 1.029542503356019E-5 1.4799673485742773E-5 9 4.31120923280333E-5 1.0938889098157701E-5 3.667745168205818E-5 10-11 5.823349784607483E-5 1.254754925965148E-5 7.59287596225064E-5 12-13 8.783284481756037E-5 1.6730065679535308E-5 8.97632370113529E-5 14-15 1.0198905423870562E-4 1.9625653970224114E-5 1.052063745616932E-4 16-17 1.1743219178904592E-4 2.445163445470545E-5 1.582921598909879E-4 18-19 1.3673611372697127E-4 2.863415087458928E-5 2.686462469694612E-4 20-21 1.5378791143880535E-4 3.2173203229875594E-5 3.445750065919676E-4 22-23 1.679441208599506E-4 3.474705948826564E-5 5.65604912781213E-4 24-25 1.788830099581083E-4 4.4720752489527075E-5 0.0017769260143860291 26-27 1.9818693189603366E-4 4.922500094170966E-5 0.0034817840535371365 28-29 2.2070817415694658E-4 5.2442321264697215E-5 0.01081277014149659 30-31 2.3936863203027442E-4 5.5981373619983535E-5 0.01984829253657485 32-33 2.5223791332222464E-4 5.7268301749178556E-5 0.031324795860703775 34-35 2.708983711955525E-4 5.9842158007568605E-5 0.05107528185945981 36-37 2.83445920455204E-4 6.305947833055616E-5 0.09771677458181045 38-39 2.9148922126267287E-4 6.402467442745242E-5 0.1810575967644156 40-41 3.088627510068057E-4 6.885065491193377E-5 0.2773741935416619 42-43 3.220537643310547E-4 7.174624320262256E-5 0.3773244318916903 44-45 3.3556650968760243E-4 7.464183149331137E-5 0.49820108681852665 46-47 3.5454869959322905E-4 7.978954401009148E-5 0.6457911523871287 48-49 3.7996553014483077E-4 8.525898855917032E-5 0.7929912787325848 50-51 3.979825239535611E-4 8.815457684985912E-5 0.9400035135711783 52-53 4.1792990995608394E-4 9.008496904365166E-5 1.0861901840148933 54-55 4.317643873449305E-4 9.426748546353548E-5 1.2390666286061962 56-57 4.4431193660458195E-4 9.941519798031558E-5 1.4111546580421548 58-59 4.629723944779098E-4 1.052063745616932E-4 1.5879618529278714 60-61 5.006150422568643E-4 1.0906715894927826E-4 1.7609864443098517 62-63 5.15414715742607E-4 1.132496753691621E-4 1.92553339664076 64-65 5.292491931314535E-4 1.1582353162755214E-4 2.088839428523092 66-67 5.456575267786901E-4 1.2032778007973472E-4 2.2682748523806566 68-69 5.636745205874204E-4 1.2322336837042352E-4 2.4539161654092325 70-71 5.810480503315532E-4 1.2611895666111235E-4 2.634656856121834 72-73 5.968129199141923E-4 1.3191013324248993E-4 2.810296807194371 74-75 6.048562207216612E-4 1.3834477388846507E-4 2.981810544952676 76-77 6.190124301428064E-4 1.4059689811455635E-4 3.1591366936064906 78-79 6.347772997254454E-4 1.4703153876053146E-4 3.3447680329420657 80-81 6.492552411788894E-4 1.4960539501892152E-4 3.53323350975016 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACTG 59620 0.0 15.189953 5 GTGTAGG 58785 0.0 14.979398 1 GTATTAG 63625 0.0 14.182137 1 GTATAAG 67790 0.0 13.901809 1 CTTACAC 49975 0.0 13.880659 3 TATACAG 59315 0.0 13.506361 5 GTGTAAG 52160 0.0 13.291842 1 GTATAGG 52415 0.0 13.002848 1 ACAGCTC 68430 0.0 12.983844 8 GTATATA 123615 0.0 12.936334 1 TACACCG 30525 0.0 12.837249 5 GTATAGA 80455 0.0 12.774346 1 ACTGTGC 46190 0.0 12.767127 8 TATACCG 23620 0.0 12.700605 5 GTATAAT 100080 0.0 12.653971 1 CTATACT 73905 0.0 12.537906 4 TTATACT 107520 0.0 12.497808 4 TCTATAC 81500 0.0 12.251622 3 TACACTG 49405 0.0 12.2498 5 CTAGGAC 52100 0.0 12.177803 3 >>END_MODULE