FastQCFastQC Report
Wed 5 Nov 2014
lane7_Undetermined_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane7_Undetermined_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155408834
Sequences flagged as poor quality0
Sequence length93
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAAC1623560.10447025167179365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG596200.015.1899535
GTGTAGG587850.014.9793981
GTATTAG636250.014.1821371
GTATAAG677900.013.9018091
CTTACAC499750.013.8806593
TATACAG593150.013.5063615
GTGTAAG521600.013.2918421
GTATAGG524150.013.0028481
ACAGCTC684300.012.9838448
GTATATA1236150.012.9363341
TACACCG305250.012.8372495
GTATAGA804550.012.7743461
ACTGTGC461900.012.7671278
TATACCG236200.012.7006055
GTATAAT1000800.012.6539711
CTATACT739050.012.5379064
TTATACT1075200.012.4978084
TCTATAC815000.012.2516223
TACACTG494050.012.24985
CTAGGAC521000.012.1778033
Produced by FastQC (version 0.11.2)