FastQCFastQC Report
Wed 5 Nov 2014
lane7_Undetermined_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane7_Undetermined_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155408834
Sequences flagged as poor quality0
Sequence length93
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAAC1624480.1045294503657366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG610450.015.9684595
TATACAG594550.014.9428295
GTGTAGG555700.014.7426971
CTTACAC521750.013.5257533
GTATAAG668550.013.4438191
TACACTG505050.013.2511435
CTATACT737400.013.2428884
GTATTAG633150.013.2138731
ACAGCTC705100.013.1970678
ACTGTGC468250.012.8836948
TACACCG314000.012.8407215
TACCGTG245050.012.6281057
GTATAGG504950.012.6094241
GTATATA1232650.012.5309391
TTATACT1083200.012.4009624
TAATACT919800.012.2729884
ATACTGT646450.012.25268656
TAGACAG437900.012.2503045
GTATAGA812450.012.21281
TCTATAC817450.012.1212693
Produced by FastQC (version 0.11.2)