##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename lane6_Undetermined_L006_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 123949988 Sequences flagged as poor quality 0 Sequence length 93 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.975889477294665 33.0 33.0 33.0 33.0 33.0 2 31.97831111528627 33.0 33.0 33.0 33.0 33.0 3 32.00379996809681 33.0 33.0 33.0 33.0 33.0 4 35.76699253895853 37.0 37.0 37.0 33.0 37.0 5 35.7064331059072 37.0 37.0 37.0 33.0 37.0 6 35.78940434427472 37.0 37.0 37.0 33.0 37.0 7 35.78528202035808 37.0 37.0 37.0 33.0 37.0 8 35.786563658239324 37.0 37.0 37.0 33.0 37.0 9 35.84063184419187 37.0 37.0 37.0 33.0 37.0 10-11 35.80990847695766 37.0 37.0 37.0 33.0 37.0 12-13 35.77581011544753 37.0 37.0 37.0 33.0 37.0 14-15 37.75975809695117 40.0 37.0 40.0 33.0 40.0 16-17 37.707627523126504 40.0 37.0 40.0 33.0 40.0 18-19 37.74986262201171 40.0 37.0 40.0 33.0 40.0 20-21 37.65057730784129 40.0 37.0 40.0 33.0 40.0 22-23 37.56196940898454 40.0 37.0 40.0 33.0 40.0 24-25 37.52008371715212 40.0 37.0 40.0 33.0 40.0 26-27 37.4729437488933 40.0 37.0 40.0 33.0 40.0 28-29 37.31970050291574 40.0 37.0 40.0 33.0 40.0 30-31 37.223395035746194 40.0 37.0 40.0 33.0 40.0 32-33 37.13456485368921 40.0 37.0 40.0 33.0 40.0 34-35 37.04388828178023 40.0 37.0 40.0 33.0 40.0 36-37 36.93160731487929 40.0 37.0 40.0 33.0 40.0 38-39 36.82612965238851 40.0 37.0 40.0 33.0 40.0 40-41 36.752741153956386 40.0 37.0 40.0 33.0 40.0 42-43 36.773383624692244 40.0 37.0 40.0 33.0 40.0 44-45 36.70159537651589 40.0 37.0 40.0 33.0 40.0 46-47 36.5677583809044 40.0 37.0 40.0 33.0 40.0 48-49 36.38283673734603 38.5 37.0 40.0 33.0 40.0 50-51 35.302889674341884 37.0 35.0 38.5 30.0 40.0 52-53 35.39141677851554 37.0 35.0 38.5 27.0 40.0 54-55 36.05874016704222 37.0 37.0 40.0 27.0 40.0 56-57 36.04019074612577 37.0 37.0 40.0 27.0 40.0 58-59 35.91257940662326 37.0 37.0 40.0 27.0 40.0 60-61 36.1031539914308 37.0 37.0 40.0 27.0 40.0 62-63 36.02948524287069 37.0 37.0 40.0 27.0 40.0 64-65 35.89328059475085 37.0 37.0 40.0 27.0 40.0 66-67 35.683295475591336 37.0 37.0 40.0 27.0 40.0 68-69 35.478490756287925 37.0 35.0 40.0 27.0 40.0 70-71 35.264472700069966 37.0 33.0 40.0 27.0 40.0 72-73 35.00708962956898 37.0 33.0 38.5 27.0 40.0 74-75 34.73396508921002 37.0 33.0 37.0 27.0 40.0 76-77 34.49901033068273 37.0 33.0 37.0 27.0 40.0 78-79 34.213822102185276 37.0 33.0 37.0 27.0 40.0 80-81 33.927222034099756 37.0 33.0 37.0 27.0 37.0 82-83 33.71588425647931 37.0 33.0 37.0 27.0 37.0 84-85 33.4942975387783 37.0 33.0 37.0 27.0 37.0 86-87 33.317854121131504 37.0 33.0 37.0 27.0 37.0 88-89 33.147181256685556 37.0 33.0 37.0 27.0 37.0 90-91 32.994122028474905 37.0 33.0 37.0 27.0 37.0 92-93 32.06758239863646 35.0 33.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2304 1 0.21545980612745552 2304 2 0.2708963805666684 2304 3 0.2827786821313545 2304 4 0.3146533261866864 2304 5 0.3171354415974008 2304 6 0.3312777911205984 2304 7 0.3910119680265538 2304 8 0.355204653214102 2304 9 0.42032465565425525 2304 10-11 0.42197456295052405 2304 12-13 0.42365957055490355 2304 14-15 0.515263557146973 2304 16-17 0.5551863326187387 2304 18-19 0.48611834705785384 2304 20-21 0.5392487245720616 2304 22-23 0.5780222557658377 2304 24-25 0.5691048404768679 2304 26-27 0.45279275166245725 2304 28-29 0.4589510596092339 2304 30-31 0.4535652264060346 2304 32-33 0.5442172996012502 2304 34-35 0.4844156035333498 2304 36-37 0.4581813016975005 2304 38-39 0.4462668460800572 2304 40-41 0.4501792739424815 2304 42-43 0.3503508275246645 2304 44-45 0.3942308371216612 2304 46-47 0.3951445195733996 2304 48-49 0.38356948066489593 2304 50-51 0.36646390837587006 2304 52-53 0.42771752620296866 2304 54-55 0.507086093462739 2304 56-57 0.5974242708770703 2304 58-59 0.5282743967905645 2304 60-61 0.4908320280010017 2304 62-63 0.5388135587358533 2304 64-65 0.5062753153688746 2304 66-67 0.5063601825046931 2304 68-69 0.5241281390452954 2304 70-71 0.5055504195986487 2304 72-73 0.5359796422545244 2304 74-75 0.48703035869057487 2304 76-77 0.49835290233954765 2304 78-79 0.4792444280842787 2304 80-81 0.41570218009430704 2304 82-83 0.39954691072130544 2304 84-85 0.34595115030648316 2304 86-87 0.31049424761935285 2304 88-89 0.371794521347681 2304 90-91 0.3685608608556805 2304 92-93 0.41134826812119485 2305 1 0.2730481668459461 2305 2 0.3382988938232856 2305 3 0.3895660574715869 2305 4 0.4242628014088794 2305 5 0.36261862048063875 2305 6 0.42796400759257835 2305 7 0.4815624700976002 2305 8 0.5677284620692404 2305 9 0.5933491772658215 2305 10-11 0.6644005678244085 2305 12-13 0.6620858664893987 2305 14-15 0.952912747951487 2305 16-17 0.9239779818989646 2305 18-19 0.891041681225957 2305 20-21 0.9426237539642486 2305 22-23 1.000917990584611 2305 24-25 0.954788346858308 2305 26-27 0.9058299585084555 2305 28-29 0.9659109524431599 2305 30-31 0.9380646530474692 2305 32-33 0.9988610526946715 2305 34-35 1.0058886982941075 2305 36-37 1.0189635182898868 2305 38-39 1.0133768757710016 2305 40-41 1.0096438550825297 2305 42-43 0.9707215377683269 2305 44-45 0.9848681827297128 2305 46-47 0.9716661460386504 2305 48-49 0.993873287033054 2305 50-51 0.9488771012335206 2305 52-53 0.9535040756665509 2305 54-55 1.0593439481032334 2305 56-57 1.1081639419514886 2305 58-59 0.9902350621277307 2305 60-61 1.060022183746078 2305 62-63 1.0544308889374463 2305 64-65 1.0208411037203504 2305 66-67 0.9843266646357449 2305 68-69 1.057593991712146 2305 70-71 1.022650387392936 2305 72-73 1.0317609739840137 2305 74-75 1.0290349079907912 2305 76-77 1.055695155121569 2305 78-79 1.0660234974173264 2305 80-81 1.0147338209586678 2305 82-83 0.9835966544814951 2305 84-85 1.0450031280138958 2305 86-87 1.0362448860605937 2305 88-89 1.0559362228686524 2305 90-91 1.0372248079184132 2305 92-93 1.0792642260941818 2306 1 0.22858361093263824 2306 2 0.2615321629221228 2306 3 0.2268699137693595 2306 4 0.264427717886214 2306 5 0.2574209645009944 2306 6 0.3594615106123058 2306 7 0.3339467556001239 2306 8 0.39052424930284957 2306 9 0.37386066381296246 2306 10-11 0.43511713297068866 2306 12-13 0.4786117907945169 2306 14-15 0.6749021535739743 2306 16-17 0.6914670630434543 2306 18-19 0.6555485010594637 2306 20-21 0.7326148728357538 2306 22-23 0.6774399527679478 2306 24-25 0.6792761075531075 2306 26-27 0.6175610489876604 2306 28-29 0.6789310461251645 2306 30-31 0.645616600115126 2306 32-33 0.6953851907287358 2306 34-35 0.7069404281630511 2306 36-37 0.7093142320706605 2306 38-39 0.6971804457116235 2306 40-41 0.6951385935676839 2306 42-43 0.6512212242371831 2306 44-45 0.6244890206183342 2306 46-47 0.618458289124753 2306 48-49 0.5881966991997061 2306 50-51 0.5424292422538883 2306 52-53 0.5963107221918449 2306 54-55 0.687228267154687 2306 56-57 0.7135822513457413 2306 58-59 0.7100901668254949 2306 60-61 0.7284307018764693 2306 62-63 0.7197413343474182 2306 64-65 0.5686506035142287 2306 66-67 0.6381719865695743 2306 68-69 0.6400312381553448 2306 70-71 0.5503453932649549 2306 72-73 0.6479258191135457 2306 74-75 0.5819894157752401 2306 76-77 0.6037383576389885 2306 78-79 0.6166425717013553 2306 80-81 0.6108583661646563 2306 82-83 0.5352961329856498 2306 84-85 0.6046385664468517 2306 86-87 0.6119319210682903 2306 88-89 0.6527350274450612 2306 90-91 0.6234484660547182 2306 92-93 0.6642906286485868 2307 1 0.2871110699597139 2307 2 0.33251268742762363 2307 3 0.3689305156728686 2307 4 0.3637673782478572 2307 5 0.3971464940101299 2307 6 0.4085944186846362 2307 7 0.5058259991208871 2307 8 0.5835205766930827 2307 9 0.5772259385485015 2307 10-11 0.6045104644756734 2307 12-13 0.6202845810877164 2307 14-15 0.8788513478152424 2307 16-17 0.8783834610261962 2307 18-19 0.87157060185951 2307 20-21 0.8839333833402563 2307 22-23 0.897899676974248 2307 24-25 0.8790947901925108 2307 26-27 0.8622631362816549 2307 28-29 0.9184997685755647 2307 30-31 0.9007090892163845 2307 32-33 0.915242985472652 2307 34-35 0.9117540981184504 2307 36-37 0.9350138432651889 2307 38-39 0.9451212755162715 2307 40-41 0.9571823579706802 2307 42-43 0.9260142034808254 2307 44-45 0.9099318175395581 2307 46-47 0.92279961536412 2307 48-49 0.89305152651756 2307 50-51 0.8535914584719393 2307 52-53 0.8993541066102608 2307 54-55 0.9716655789229307 2307 56-57 1.0017210515814838 2307 58-59 0.9960870970155185 2307 60-61 1.0193219329863226 2307 62-63 1.022917273777196 2307 64-65 0.9946808629801396 2307 66-67 0.9715312512283703 2307 68-69 0.9666604289000134 2307 70-71 0.9874294375984789 2307 72-73 0.9984357866915659 2307 74-75 0.9490833437721165 2307 76-77 0.9450184411015528 2307 78-79 0.9605801830269272 2307 80-81 0.9626781284663721 2307 82-83 0.9337095330939604 2307 84-85 0.9583244990934929 2307 86-87 0.9769303601571266 2307 88-89 0.9770552575464819 2307 90-91 0.974032095820057 2307 92-93 1.0048094161619474 2308 1 0.26739729881690266 2308 2 0.31368936523563207 2308 3 0.28962712307768257 2308 4 0.3708679463857223 2308 5 0.4244323412979938 2308 6 0.4625118358398268 2308 7 0.44862455638270404 2308 8 0.48779711196193887 2308 9 0.5016087328936152 2308 10-11 0.5096353792999508 2308 12-13 0.536210482281966 2308 14-15 0.7098544954243664 2308 16-17 0.7634938121403181 2308 18-19 0.6922714250754751 2308 20-21 0.7486493512709984 2308 22-23 0.779657896096893 2308 24-25 0.7803943704889349 2308 26-27 0.7049453536608965 2308 28-29 0.7689196041143518 2308 30-31 0.7528611210674612 2308 32-33 0.7412795364433933 2308 34-35 0.7147206689895853 2308 36-37 0.7977244861487733 2308 38-39 0.7628158812013766 2308 40-41 0.7814487090840174 2308 42-43 0.6995927110464564 2308 44-45 0.7153884973758409 2308 46-47 0.7066400864779041 2308 48-49 0.7047610855455204 2308 50-51 0.6633661531019612 2308 52-53 0.7212132090171934 2308 54-55 0.8189929442486772 2308 56-57 0.6649954147067376 2308 58-59 0.7898921908306065 2308 60-61 0.7550401182569502 2308 62-63 0.7493576369493766 2308 64-65 0.7404622784268398 2308 66-67 0.7969408950320229 2308 68-69 0.7736344414005245 2308 70-71 0.7309067391406998 2308 72-73 0.8181086584926263 2308 74-75 0.8420531561432156 2308 76-77 0.8140775196832948 2308 78-79 0.7596404891972952 2308 80-81 0.8019008690127052 2308 82-83 0.7650670939655555 2308 84-85 0.7765136314618033 2308 86-87 0.7811772528769723 2308 88-89 0.8422343425185659 2308 90-91 0.8262259414213204 2308 92-93 0.8538728583151425 2309 1 0.3193040081791665 2309 2 0.31774183832258274 2309 3 0.3070940995554601 2309 4 0.3506750015321529 2309 5 0.4308614149942187 2309 6 0.44898578887020335 2309 7 0.4403818994067379 2309 8 0.5139651323737624 2309 9 0.44370343024258574 2309 10-11 0.4886794761307698 2309 12-13 0.522944182854701 2309 14-15 0.6657855399001775 2309 16-17 0.6386927567483625 2309 18-19 0.6235689321265099 2309 20-21 0.6947978114192495 2309 22-23 0.7321243426378601 2309 24-25 0.7116775890672642 2309 26-27 0.6639287074753213 2309 28-29 0.692276900327478 2309 30-31 0.6654075686729612 2309 32-33 0.634567387427758 2309 34-35 0.6819174797593703 2309 36-37 0.7353663099255741 2309 38-39 0.6736049242696183 2309 40-41 0.7069972363881263 2309 42-43 0.6611829666959395 2309 44-45 0.671723157099791 2309 46-47 0.648827750939823 2309 48-49 0.6505024472232819 2309 50-51 0.6992169391737946 2309 52-53 0.673917819690999 2309 54-55 0.7450496426040658 2309 56-57 0.7530377400432542 2309 58-59 0.7763605502698212 2309 60-61 0.779656557599111 2309 62-63 0.7707902632992827 2309 64-65 0.7353878821792321 2309 66-67 0.7487609154204335 2309 68-69 0.7413898438455817 2309 70-71 0.751422625010953 2309 72-73 0.7242528881333072 2309 74-75 0.7302849155337228 2309 76-77 0.7423670895258212 2309 78-79 0.6717616167522422 2309 80-81 0.6993566991114548 2309 82-83 0.6744463499552324 2309 84-85 0.715945271229252 2309 86-87 0.7353168299237183 2309 88-89 0.7585893790538876 2309 90-91 0.7917212501290365 2309 92-93 0.8407909618531946 2310 1 0.2586856521730496 2310 2 0.290553771145305 2310 3 0.25120484129523746 2310 4 0.3289494719788877 2310 5 0.3476196758518384 2310 6 0.4143813238716092 2310 7 0.3777472494255889 2310 8 0.4162127072694801 2310 9 0.39173466888597375 2310 10-11 0.4087712776626944 2310 12-13 0.4425570586440273 2310 14-15 0.5578283356285425 2310 16-17 0.5605351254639359 2310 18-19 0.5080785694584833 2310 20-21 0.5194730645448686 2310 22-23 0.5222330515724423 2310 24-25 0.5435682497052099 2310 26-27 0.47214849998115227 2310 28-29 0.48976342502506753 2310 30-31 0.39246765998876043 2310 32-33 0.5171809508422456 2310 34-35 0.48237178073406994 2310 36-37 0.5168376183849475 2310 38-39 0.48714645928279765 2310 40-41 0.5549235347659192 2310 42-43 0.5410272000860559 2310 44-45 0.4950498598296207 2310 46-47 0.49238719039423273 2310 48-49 0.5646703257966479 2310 50-51 0.48375239356370514 2310 52-53 0.557942434357642 2310 54-55 0.578339716349241 2310 56-57 0.5872552793050545 2310 58-59 0.6046547058327576 2310 60-61 0.5619365642305567 2310 62-63 0.604741609836168 2310 64-65 0.5389263119443726 2310 66-67 0.5850847576768103 2310 68-69 0.5098770825920553 2310 70-71 0.5679287937982522 2310 72-73 0.5823474673327453 2310 74-75 0.5778544074727989 2310 76-77 0.576851755352493 2310 78-79 0.5555655361002678 2310 80-81 0.46924616004783815 2310 82-83 0.4873193236215698 2310 84-85 0.5415751590244682 2310 86-87 0.4680570603440657 2310 88-89 0.5523347547581352 2310 90-91 0.5582913368090843 2310 92-93 0.641169659605012 2311 1 0.017635014810878147 2311 2 0.026087278824991955 2311 3 -0.010834743336108232 2311 4 0.03879275036493368 2311 5 0.07089851148973736 2311 6 0.09839655383566992 2311 7 0.05077859346789637 2311 8 0.09885256550250432 2311 9 0.0602687247781617 2311 10-11 0.0844846667002912 2311 12-13 0.0903588770273629 2311 14-15 0.048244328514456924 2311 16-17 0.0832445101378525 2311 18-19 -0.0048212642850629095 2311 20-21 0.10308945982431084 2311 22-23 0.0727916608409771 2311 24-25 0.035041787886072484 2311 26-27 -0.019884756246135282 2311 28-29 -0.002671642802688723 2311 30-31 -0.07027874183507521 2311 32-33 -0.03781526034368454 2311 34-35 -0.012159871381967946 2311 36-37 0.009246901037414545 2311 38-39 -0.08106017129519216 2311 40-41 -0.05385563186823816 2311 42-43 -0.11236452549737663 2311 44-45 -0.1901354923307963 2311 46-47 -0.14747203641324091 2311 48-49 -0.1275490492288256 2311 50-51 -0.1071561154788867 2311 52-53 -0.10242224494888319 2311 54-55 -0.058655811890922394 2311 56-57 -0.03117121479839824 2311 58-59 0.015619661781940408 2311 60-61 -0.030794351696535216 2311 62-63 0.03612147286314382 2311 64-65 -0.014432942138959959 2311 66-67 -0.09546053365025386 2311 68-69 -0.0051349206261050995 2311 70-71 0.050137920680946024 2311 72-73 -0.022033324885832428 2311 74-75 -0.0503597389393633 2311 76-77 -0.017195949388849385 2311 78-79 -0.0242475006530114 2311 80-81 0.0011633999331834843 2311 82-83 -0.04842791764058063 2311 84-85 -0.086367244246631 2311 86-87 -0.06519901371201797 2311 88-89 -0.05450957925354061 2311 90-91 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-0.5143361457619839 1116 3 -0.5054671224178975 1116 4 -0.9710632473375469 1116 5 -0.6243890327588986 1116 6 -0.6339760897913109 1116 7 -0.7897961842832331 1116 8 -0.9587296105730232 1116 9 -0.7625438397752689 1116 10-11 -1.0712078399534946 1116 12-13 -1.0672068986179895 1116 14-15 -1.9819968761763604 1116 16-17 -1.4738228923844616 1116 18-19 -1.1076560817214087 1116 20-21 -1.6056370307453989 1116 22-23 -1.243930455957539 1116 24-25 -1.5559145379651937 1116 26-27 -1.23381223825767 1116 28-29 -1.2931420636186957 1116 30-31 -1.057781420277479 1116 32-33 -0.9743105567157357 1116 34-35 -1.3219305597372113 1116 36-37 -1.3635303174358668 1116 38-39 -1.2364001423829976 1116 40-41 -1.2471580618961582 1116 42-43 -1.5708090934372834 1116 44-45 -1.2925504864253625 1116 46-47 -1.0879749965464427 1116 48-49 -1.0292714101972038 1116 50-51 -0.8741901318322505 1116 52-53 -0.911041150245822 1116 54-55 -1.1009567326644571 1116 56-57 -1.1349696381928993 1116 58-59 -2.3604459156490236 1116 60-61 -1.6618378952649024 1116 62-63 -1.227536662999114 1116 64-65 -1.1341713569949619 1116 66-67 -1.1416911274179142 1116 68-69 -1.1130166227992078 1116 70-71 -1.0345701797284264 1116 72-73 -1.0689808427139909 1116 74-75 -1.138132919740535 1116 76-77 -1.9884340207025986 1116 78-79 -1.4897305651469637 1116 80-81 -1.159775434703782 1116 82-83 -1.0353041546262602 1116 84-85 -1.2838119928723586 1116 86-87 -1.1318202945125364 1116 88-89 -1.6006405446279217 1116 90-91 -1.3667640199250712 1116 92-93 -1.1977770605655564 2302 1 -0.23034311457176315 2302 2 -0.1484770941244875 2302 3 -0.14813970175246283 2302 4 -0.07154594864154973 2302 5 -0.6142826953173071 2302 6 -0.35791830754324394 2302 7 -0.18249071465337607 2302 8 -0.8124162054363353 2302 9 -0.44588919104403146 2302 10-11 -0.15804546376889306 2302 12-13 -0.13773535188996533 2302 14-15 -0.2523539865575657 2302 16-17 -0.17363075307007847 2302 18-19 -0.2575802226207813 2302 20-21 -0.6721373696564896 2302 22-23 -1.4504808911659808 2302 24-25 -1.6690797061702227 2302 26-27 -0.8235753742466798 2302 28-29 -0.4112543007777347 2302 30-31 -0.5291298911274609 2302 32-33 -0.4101897011093669 2302 34-35 -0.8602161941368038 2302 36-37 -0.5611328667696895 2302 38-39 -0.38803693884742074 2302 40-41 -0.25169972531608664 2302 42-43 -0.2736531426462889 2302 44-45 -0.6351108340433314 2302 46-47 -0.6616373104396942 2302 48-49 -0.4787268803126068 2302 50-51 -0.9608249298904994 2302 52-53 -0.46036048746805847 2302 54-55 -0.3201194683795663 2302 56-57 -0.7721870651953822 2302 58-59 -0.6772901382654197 2302 60-61 -0.5624381797544586 2302 62-63 -0.4719411224603789 2302 64-65 -0.44575880349501773 2302 66-67 -0.3914998344202587 2302 68-69 -0.41573255691336186 2302 70-71 -0.39667362047583055 2302 72-73 -0.3576384392222849 2302 74-75 -0.4391252431013868 2302 76-77 -0.6751170081229105 2302 78-79 -1.0395222335320753 2302 80-81 -0.5915761370827042 2302 82-83 -0.8112725684906152 2302 84-85 -0.4847201671643617 2302 86-87 -0.4345951060713915 2302 88-89 -0.44091212587537143 2302 90-91 -0.4863637295067136 2302 92-93 -0.675000573755856 2303 1 -0.4653085557949588 2303 2 -0.504906818284443 2303 3 -0.6132562430032742 2303 4 -0.705607641599002 2303 5 -0.49280907780914873 2303 6 -0.46362183298997905 2303 7 -0.5916053694323438 2303 8 -0.5000699398074389 2303 9 -0.7720314783539521 2303 10-11 -0.6984903357784376 2303 12-13 -0.744004433804939 2303 14-15 -1.0752002848038416 2303 16-17 -1.0461590086522747 2303 18-19 -1.1034762904060713 2303 20-21 -1.070393350661341 2303 22-23 -0.9899230457828452 2303 24-25 -1.266978312174679 2303 26-27 -1.1433171064770917 2303 28-29 -1.0832554968037726 2303 30-31 -0.9498208251962694 2303 32-33 -1.1369691061565632 2303 34-35 -1.0614920964772239 2303 36-37 -1.714022705782675 2303 38-39 -1.580166033451654 2303 40-41 -1.256591124976282 2303 42-43 -0.99871130725829 2303 44-45 -0.8228656786414632 2303 46-47 -0.9346651696227397 2303 48-49 -0.8918684422341201 2303 50-51 -0.855899660165349 2303 52-53 -1.3573110897801257 2303 54-55 -1.4851954938530056 2303 56-57 -1.3245416524706002 2303 58-59 -1.4442292620875676 2303 60-61 -1.5195368174014874 2303 62-63 -1.6298955240195951 2303 64-65 -1.435317013150076 2303 66-67 -1.3259336609267436 2303 68-69 -1.1740571626113478 2303 70-71 -1.0852360801211134 2303 72-73 -1.1679525453747601 2303 74-75 -0.9963452559102421 2303 76-77 -1.2492824862579397 2303 78-79 -1.2479753597658103 2303 80-81 -1.2809184730561682 2303 82-83 -1.2028725466856471 2303 84-85 -1.0990618642389478 2303 86-87 -1.0662718681163454 2303 88-89 -0.9908252892404263 2303 90-91 -0.9268290110496125 2303 92-93 -1.0720037171138017 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 308363.0 3 16561.0 4 12315.0 5 7864.0 6 8140.0 7 18604.0 8 37663.0 9 44505.0 10 55476.0 11 89217.0 12 143704.0 13 194936.0 14 230800.0 15 256690.0 16 282735.0 17 311288.0 18 344636.0 19 383534.0 20 424603.0 21 473714.0 22 533632.0 23 601052.0 24 688602.0 25 789174.0 26 913054.0 27 1068089.0 28 1262640.0 29 1508975.0 30 1856188.0 31 2351207.0 32 3066734.0 33 4145423.0 34 5859177.0 35 8848335.0 36 1.4938857E7 37 2.9453459E7 38 4.0441296E7 39 1978746.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.51302695025705 23.616287899524792 11.244790659265743 24.625894490952412 2 15.572833838766813 22.39142020699324 38.998610591968955 23.037135362271 3 15.338465050784627 27.22021750447718 31.84773018059859 25.593587264139607 4 11.43238405852391 16.99797169156717 42.49324043799567 29.07640381191325 5 11.985042924075087 38.421782692013586 38.00755427660147 11.585620107309863 6 28.566286596798456 41.90463110937387 18.298462562535633 11.230619731292045 7 25.96755031735633 32.804017877305505 26.214412246106438 15.014019559231729 8 23.2025867866867 37.78098639629393 22.84534558114387 16.17108123587549 9 23.72959466688259 15.115516060992757 24.914270242562804 36.24061902956184 10-11 22.511519914595592 27.111341422300168 31.687448115498253 18.689690547605984 12-13 21.992866489296723 25.382626379320524 31.637187205692946 20.987319925689814 14-15 19.904592660052067 26.96433306005937 27.24793882325948 25.88313545662908 16-17 20.04500116483924 31.50256059373349 28.337860576348973 20.114577665078304 18-19 20.0862601578621 30.260447656403226 29.900662332147736 19.752629853586935 20-21 20.40785557567754 29.817892176966243 29.681746764816598 20.092505482539618 22-23 20.452990039709594 29.604090923675138 29.674899871800807 20.268019164814465 24-25 20.03170552599045 29.465614616137113 29.98727944462784 20.515400413244592 26-27 20.312294081078402 30.060996114263734 29.638450765322094 19.98825903933577 28-29 20.375248866173752 29.900625907088816 29.63298697170849 20.09113825502894 30-31 20.11696763428816 29.778997179766225 29.82791866087201 20.276116525073608 32-33 20.317781944959506 30.008859035809838 29.674766934259278 19.998592084971378 34-35 20.182501609498242 29.70656115162884 29.819594134010885 20.291343104862033 36-37 20.136792209494203 29.928540622863252 29.830836005332106 20.103831162310435 38-39 20.218109030869872 29.928130518714045 29.82686528205417 20.026895168361907 40-41 20.081812432377642 29.826627444684533 29.973070394994068 20.118489727943757 42-43 20.08256101138295 29.862051550357133 29.978060251946314 20.077327186313607 44-45 20.060495118815403 29.7313334930142 29.94467521917421 20.263496168996188 46-47 19.99689096812038 29.69380296319092 30.122406255541744 20.186899813146958 48-49 20.071213118554248 29.92446959361816 29.95578095521063 20.048536332616962 50-51 19.992707590327598 29.868880318100143 30.053498699387276 20.084913392184983 52-53 20.026666776004728 29.74246227837899 30.19861530010422 20.03225564551206 54-55 20.04750411482612 29.826117319227425 30.092878949856534 20.033499616089923 56-57 20.004112927581012 29.840925603163303 29.99943565997707 20.155525809278615 58-59 20.03865242769184 29.85863007573742 29.978916231808668 20.123801264762072 60-61 19.889191594794443 29.78689652499545 30.14676735371957 20.17714452649053 62-63 19.902666458143536 29.89701335061684 30.04253509966299 20.15778509157663 64-65 19.95891165767187 29.76627161105922 30.126402756250748 20.148413975018162 66-67 19.92875427729126 29.704077800185768 30.084191620560148 20.282976301962826 68-69 19.903592493311038 29.876528042700564 30.009505351323444 20.210374112664955 70-71 20.016806634272285 29.789871140773926 29.99280120350895 20.200521021444835 72-73 19.99048770861451 29.811911968504152 29.929976924342956 20.267623398538387 74-75 19.956415720839882 29.88757691102286 29.964830902192336 20.19117646594492 76-77 19.975179579860793 29.862651327869404 29.95127648540066 20.210892606869148 78-79 20.09674498523696 29.8172948676227 29.897651000073562 20.18830914706678 80-81 20.037527948380326 29.936083238522688 29.812322673773494 20.21406613932349 82-83 20.114164429516205 29.934267443121993 29.85234341232539 20.099224715036417 84-85 20.093062365781527 29.9654458957886 29.843818957188567 20.097672781241304 86-87 19.999218492407817 29.850848713359028 29.89473433575182 20.255198458481335 88-89 20.12700955057166 29.859057360967185 29.8118285211462 20.20210456731496 90-91 20.22870530264442 29.957831131826694 29.760935552368302 20.052528013160583 92-93 20.333248299769757 29.84193192760585 29.70849567032044 20.116324102303953 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 291063.0 1 152190.0 2 12306.5 3 10500.5 4 9064.0 5 8194.5 6 8051.0 7 8384.5 8 9264.5 9 10845.0 10 13043.0 11 16512.5 12 21838.5 13 30108.0 14 35272.0 15 43576.5 16 65080.5 17 98661.5 18 151778.0 19 233380.5 20 351150.5 21 514109.5 22 740125.5 23 1030781.0 24 1371279.0 25 1768955.5 26 2233452.5 27 2735686.0 28 3241490.0 29 3493683.0 30 3729593.5 31 4177596.5 32 4569835.5 33 4893141.0 34 5158688.0 35 5362352.5 36 5466530.0 37 5478893.0 38 5406958.5 39 5261303.0 40 5075297.0 41 4848269.0 42 4594195.0 43 4465402.0 44 4327042.0 45 4002283.0 46 3665276.5 47 3365827.5 48 3070725.5 49 2788363.5 50 2530689.5 51 2303262.0 52 2085791.5 53 1878913.0 54 1697660.5 55 1528884.5 56 1376893.5 57 1307689.0 58 1249409.5 59 1137083.5 60 1029200.5 61 927487.5 62 832356.0 63 741394.5 64 660756.0 65 584925.0 66 511320.0 67 440908.0 68 377236.5 69 316417.5 70 258655.0 71 233746.0 72 213609.5 73 175252.5 74 142322.0 75 120751.0 76 107788.0 77 92402.5 78 75943.5 79 61107.0 80 51247.5 81 44483.5 82 36172.0 83 27107.0 84 18692.0 85 12819.0 86 10042.0 87 8149.0 88 5013.5 89 3185.5 90 2202.0 91 1521.0 92 1044.0 93 718.0 94 516.5 95 381.5 96 272.0 97 225.5 98 175.5 99 98.0 100 68.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.48187741655933036 2 0.48201295509605047 3 0.4908713666031174 4 0.48996132214228205 5 0.487903234004347 6 0.3212836131940569 7 0.3236531172556467 8 0.3099435556218045 9 0.3042235066614125 10-11 0.3011097508133684 12-13 0.2877821980910559 14-15 0.2636506911158394 16-17 0.26271281284835624 18-19 0.2672622283755284 20-21 0.2819056343918323 22-23 0.28846916871020595 24-25 0.29159946348683796 26-27 0.30385198585093853 28-29 0.30925819855666303 30-31 0.3160879692864512 32-33 0.3157684855927538 34-35 0.30503552771622694 36-37 0.29302019779138666 38-39 0.3025446037154921 40-41 0.3013614652387058 42-43 0.2901896206718471 44-45 0.2868523875936156 46-47 0.29698227965943813 48-49 0.29484069010155933 50-51 0.31243609317654797 52-53 0.33783343327149007 54-55 0.3691230692172395 56-57 0.3859032241293964 58-59 0.38276082769770015 60-61 0.35137639545394717 62-63 0.34217228000054345 64-65 0.3385014446310394 66-67 0.328352189917114 68-69 0.3202529555710808 70-71 0.318364693992548 72-73 0.34166562404185147 74-75 0.3491287147200046 76-77 0.35400003427188714 78-79 0.3804328726518312 80-81 0.3987616360237162 82-83 0.3902969316947413 84-85 0.3781432395136658 86-87 0.3487809938311571 88-89 0.31882617043899997 90-91 0.3108136646209276 92-93 0.30274629796656377 >>END_MODULE >>Sequence Length Distribution pass #Length Count 93 1.23949988E8 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.90337329682008 #Duplication Level Percentage of deduplicated Percentage of total 1 80.4726818971905 49.815304676780386 2 12.521962071917377 15.503033850930478 3 3.253261150554973 6.041635183045406 4 1.3460466955713692 3.332993242836224 5 0.6090175882133836 1.8850121553751071 6 0.31930762007761715 1.185973128131036 7 0.21319874471144312 0.9238405036200135 8 0.2067230311975548 1.0237482375417934 9 0.12997640435041247 0.7241380090453811 >10 0.7988445086090743 9.418348236228542 >50 0.07518080991068708 3.2277504108479294 >100 0.051714936157059924 5.7442397761901995 >500 0.0016225357574816308 0.6243647087967583 >1k 4.505129082663942E-4 0.43645541694947554 >5k 4.260543030093238E-6 0.014898784768748615 >10k+ 7.2323298556288396E-6 0.09826367891260149 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 1.6135540085731996E-6 0.0 2 2.4203310128597996E-6 2.4203310128597996E-6 0.0 3 3.2271080171463992E-6 4.033885021432999E-6 1.6135540085731996E-6 4 3.2271080171463992E-6 6.4542160342927984E-6 6.4542160342927984E-6 5 1.1294878060012399E-5 6.4542160342927984E-6 6.4542160342927984E-6 6 1.9362648102878397E-5 7.2609930385793985E-6 8.067770042865998E-6 7 2.3396533124311395E-5 8.067770042865998E-6 8.874547047152598E-6 8 3.4691411184323796E-5 9.681324051439199E-6 9.681324051439199E-6 9 3.711174219718359E-5 1.1294878060012399E-5 2.0976202111451595E-5 10-11 4.356595823147639E-5 1.3715209072872198E-5 8.793869346723939E-5 12-13 5.486083629148879E-5 1.9766036605021694E-5 1.0326745654868478E-4 14-15 6.454216034292799E-5 2.3396533124311398E-5 1.1859621963013018E-4 16-17 7.341670739008058E-5 2.5413475635027893E-5 1.5490118482302715E-4 18-19 8.269464293937648E-5 2.9447360656460894E-5 2.0008069706307674E-4 20-21 9.398952099938888E-5 3.4691411184323796E-5 2.5292459084384907E-4 22-23 1.0367084505082808E-4 3.953207321004339E-5 3.4530055783466474E-4 24-25 1.1173861509369408E-4 4.275918122718979E-5 6.958451661971924E-4 26-27 1.2182332764727657E-4 4.80032317550527E-5 0.0019443325803307056 28-29 1.3594192522229208E-4 5.203711677648569E-5 0.008920936724899077 30-31 1.5127068830373747E-4 5.526422479363209E-5 0.016456637333438065 32-33 1.6175878935946328E-4 5.768455580649189E-5 0.025171442533741914 34-35 1.7224689041518907E-4 6.131505232578158E-5 0.04202985481531471 36-37 1.8596209948806126E-4 6.494554884507129E-5 0.08137152865234645 38-39 1.9685358904593037E-4 6.817265686221769E-5 0.1508535845925213 40-41 2.0653491309736955E-4 7.099637637722078E-5 0.23332434691320825 42-43 2.1379590613594896E-4 7.260993038579398E-5 0.32213234260256646 44-45 2.2468739569381804E-4 7.422348439436718E-5 0.43146595544648214 46-47 2.3638566225597375E-4 7.785398091365689E-5 0.5660303089339549 48-49 2.4848731732027274E-4 8.067770042865999E-5 0.7038915566494448 50-51 2.6220252639314496E-4 8.350141994366308E-5 0.8418254142953204 52-53 2.755143469638739E-4 8.713191646295278E-5 0.9793558834390528 54-55 2.9084311004531926E-4 9.076241298224249E-5 1.1277927675152337 56-57 3.0818881563748115E-4 9.237596699081568E-5 1.2972853212377884 58-59 3.307785717575059E-4 9.439290950153218E-5 1.4723301143038432 60-61 3.686970909589761E-4 9.883018302510848E-5 1.6434321881499496 62-63 3.7797502650827204E-4 1.0407423355297138E-4 1.8077169963098343 64-65 3.9693428610900714E-4 1.0447762205511468E-4 1.9739796182957274 66-67 4.0661561016044635E-4 1.0810811857440438E-4 2.156288631508379 68-69 4.162969342118855E-4 1.1173861509369408E-4 2.3457174517838597 70-71 4.296087547826144E-4 1.1496572311084047E-4 2.5307384458964206 72-73 4.4171040984691346E-4 1.1899960813227348E-4 2.70988327969826 74-75 4.542154534133557E-4 1.2222671614941987E-4 2.884696931152587 76-77 4.614764464519351E-4 1.2424365866013637E-4 3.0688603213095913 78-79 4.711577705033743E-4 1.3029448619228588E-4 3.262238315021055 80-81 4.848729795762465E-4 1.3473175971586218E-4 3.4551346628609596 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACTG 52180 0.0 15.833104 5 GTGTAGG 42715 0.0 14.4510355 1 GTATATA 106935 0.0 14.086874 1 TATACAG 50000 0.0 14.075835 5 GTATTAG 54860 0.0 13.872241 1 GTATAGG 41800 0.0 13.860691 1 CTTACAC 40230 0.0 13.805447 3 TACACCG 23565 0.0 13.787141 5 TACACTG 37035 0.0 13.64107 5 CTATACT 59560 0.0 13.573107 4 GTATAAG 57085 0.0 13.499427 1 GTATAGA 66250 0.0 13.150844 1 TATACCG 19555 0.0 13.073236 5 GTGTAAG 39720 0.0 12.985299 1 TTATACT 93780 0.0 12.763286 4 TCTATAC 65745 0.0 12.674843 3 GTATAAT 83035 0.0 12.517534 1 TAATACT 79235 0.0 12.225698 4 CCTACAC 43510 0.0 12.16403 3 ACTGTGC 36380 0.0 12.094939 8 >>END_MODULE