FastQCFastQC Report
Wed 5 Nov 2014
lane6_Undetermined_L006_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane6_Undetermined_L006_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123949988
Sequences flagged as poor quality0
Sequence length93
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG521800.015.8331045
GTGTAGG427150.014.45103551
GTATATA1069350.014.0868741
TATACAG500000.014.0758355
GTATTAG548600.013.8722411
GTATAGG418000.013.8606911
CTTACAC402300.013.8054473
TACACCG235650.013.7871415
TACACTG370350.013.641075
CTATACT595600.013.5731074
GTATAAG570850.013.4994271
GTATAGA662500.013.1508441
TATACCG195550.013.0732365
GTGTAAG397200.012.9852991
TTATACT937800.012.7632864
TCTATAC657450.012.6748433
GTATAAT830350.012.5175341
TAATACT792350.012.2256984
CCTACAC435100.012.164033
ACTGTGC363800.012.0949398
Produced by FastQC (version 0.11.2)