FastQCFastQC Report
Wed 5 Nov 2014
lane6_Undetermined_L006_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane6_Undetermined_L006_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123949988
Sequences flagged as poor quality0
Sequence length93
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG524950.016.4375175
TACACTG374750.014.7947845
TATACAG513600.014.7819745
CTATACT613000.013.8287674
GTGTAGG417550.013.7558871
TCTATAC652150.013.4975193
TATACCG200150.013.373625
TACACCG234050.013.3086865
CTTACAC397200.013.2377073
GTATTAG537050.013.2272231
GTATAGG418800.013.0923711
GTATATA1072000.012.9491631
GTATAAG551400.012.9460251
TAATACT803700.012.5933174
ACTGTGC371050.012.4168518
TTATACT941250.012.393824
GTATAGA664800.012.2996921
TAGACAG370050.012.2276585
CTAGTAC347150.012.2219663
CCTACAC440900.012.0634013
Produced by FastQC (version 0.11.2)