##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename lane5_Undetermined_L005_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 110610181 Sequences flagged as poor quality 0 Sequence length 93 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.002079012961744 33.0 33.0 33.0 33.0 33.0 2 32.00909824024246 33.0 33.0 33.0 33.0 33.0 3 32.019940714137334 33.0 33.0 33.0 33.0 33.0 4 35.79170656089967 37.0 37.0 37.0 33.0 37.0 5 35.74386882162321 37.0 37.0 37.0 33.0 37.0 6 35.82963383813647 37.0 37.0 37.0 33.0 37.0 7 35.842401650169975 37.0 37.0 37.0 33.0 37.0 8 35.87030542875615 37.0 37.0 37.0 33.0 37.0 9 35.86947365179702 37.0 37.0 37.0 33.0 37.0 10-11 35.85071876882653 37.0 37.0 37.0 33.0 37.0 12-13 35.848440059057495 37.0 37.0 37.0 33.0 37.0 14-15 37.8158218319885 40.0 37.0 40.0 33.0 40.0 16-17 37.740602047292555 40.0 37.0 40.0 33.0 40.0 18-19 37.77350074583099 40.0 37.0 40.0 33.0 40.0 20-21 37.73296168821928 40.0 37.0 40.0 33.0 40.0 22-23 37.63214892488061 40.0 37.0 40.0 33.0 40.0 24-25 37.57273523492381 40.0 37.0 40.0 33.0 40.0 26-27 37.46073875423818 40.0 37.0 40.0 33.0 40.0 28-29 37.39505890059071 40.0 37.0 40.0 33.0 40.0 30-31 37.30644290781876 40.0 37.0 40.0 33.0 40.0 32-33 37.215155298407836 40.0 37.0 40.0 33.0 40.0 34-35 37.13626735679964 40.0 37.0 40.0 33.0 40.0 36-37 37.023026072075595 40.0 37.0 40.0 33.0 40.0 38-39 36.915826921031794 40.0 37.0 40.0 33.0 40.0 40-41 36.85168723302243 40.0 37.0 40.0 33.0 40.0 42-43 36.84616134024769 40.0 37.0 40.0 33.0 40.0 44-45 36.76135290837287 40.0 37.0 40.0 33.0 40.0 46-47 36.636654292248195 40.0 37.0 40.0 33.0 40.0 48-49 36.47274596720894 40.0 37.0 40.0 33.0 40.0 50-51 35.374695761504995 38.5 35.0 38.5 30.0 40.0 52-53 35.46489466914443 37.0 37.0 38.5 27.0 40.0 54-55 36.13768130892038 37.0 37.0 40.0 30.0 40.0 56-57 36.12371102620291 37.0 37.0 40.0 27.0 40.0 58-59 35.985588049078416 37.0 37.0 40.0 27.0 40.0 60-61 36.16339358037937 37.0 37.0 40.0 30.0 40.0 62-63 36.13563032231183 37.0 37.0 40.0 33.0 40.0 64-65 35.98131825677059 37.0 37.0 40.0 27.0 40.0 66-67 35.79892866733488 37.0 37.0 40.0 27.0 40.0 68-69 35.54896051114861 37.0 37.0 40.0 27.0 40.0 70-71 35.31156657722131 37.0 33.0 40.0 27.0 40.0 72-73 35.033072706028754 37.0 33.0 38.5 27.0 40.0 74-75 34.7606635504918 37.0 33.0 37.0 27.0 40.0 76-77 34.51366832588403 37.0 33.0 37.0 27.0 40.0 78-79 34.23793789380021 37.0 33.0 37.0 27.0 40.0 80-81 33.989077370734975 37.0 33.0 37.0 27.0 37.0 82-83 33.737098762183564 37.0 33.0 37.0 27.0 37.0 84-85 33.5381799754943 37.0 33.0 37.0 27.0 37.0 86-87 33.35853851464179 37.0 33.0 37.0 27.0 37.0 88-89 33.1970468342331 37.0 33.0 37.0 27.0 37.0 90-91 33.02809862050583 37.0 33.0 37.0 27.0 37.0 92-93 32.16729704564899 35.0 33.0 37.0 24.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2304 1 -0.2366319012739453 2304 2 -0.15040569751484512 2304 3 -0.10294175136856154 2304 4 -0.21656950811328812 2304 5 -0.22356988429294944 2304 6 -0.1785118118584137 2304 7 -0.11531053352210563 2304 8 -0.0166533314574977 2304 9 0.02919162908296613 2304 10-11 0.04645417217212611 2304 12-13 0.047493329211711455 2304 14-15 0.25958120168617427 2304 16-17 0.24636272053935926 2304 18-19 0.250837841240255 2304 20-21 0.12403669883153867 2304 22-23 0.1607769554841596 2304 24-25 0.13207511276743844 2304 26-27 0.12982005096621663 2304 28-29 0.0906600050983144 2304 30-31 0.07372061939187091 2304 32-33 0.016104610229028538 2304 34-35 0.0485737411474787 2304 36-37 0.039221589416108316 2304 38-39 0.04752740716832449 2304 40-41 0.050916989478231756 2304 42-43 0.07858906088760875 2304 44-45 0.0690158969674286 2304 46-47 0.004583326779176389 2304 48-49 -0.024612232253048205 2304 50-51 -0.14696696360362438 2304 52-53 -0.06387867251460477 2304 54-55 -0.036758642391653495 2304 56-57 -0.12609024058280482 2304 58-59 -0.18571841906908304 2304 60-61 -0.11092579693035987 2304 62-63 -0.06579602724263367 2304 64-65 -0.024782688916403117 2304 66-67 -0.03483861221185691 2304 68-69 0.058136878401306546 2304 70-71 0.06168362256790516 2304 72-73 0.014035604168832094 2304 74-75 0.0017443905123144532 2304 76-77 -0.13527085265766203 2304 78-79 -0.27053868511325163 2304 80-81 -0.2740297567402763 2304 82-83 -0.25186412376545064 2304 84-85 -0.1982769402885296 2304 86-87 -0.14650225050017696 2304 88-89 -0.20496605820839875 2304 90-91 -0.13797039133572753 2304 92-93 -0.22065476435475517 2305 1 0.25018608029846234 2305 2 0.28415805104233627 2305 3 0.3403548135327412 2305 4 0.31730202032220234 2305 5 0.26722006153814704 2305 6 0.28938913956688594 2305 7 0.40234329569793204 2305 8 0.45750796061902577 2305 9 0.5811325331604422 2305 10-11 0.6070835205323988 2305 12-13 0.6154403303255194 2305 14-15 0.9856481670149719 2305 16-17 1.0196313553034955 2305 18-19 0.9873318228835757 2305 20-21 0.9511848150570827 2305 22-23 1.0191474063189858 2305 24-25 0.9741727774960012 2305 26-27 1.0044976413151758 2305 28-29 1.0113678368364987 2305 30-31 1.0460291417052616 2305 32-33 1.0099325282092906 2305 34-35 1.0310487967483155 2305 36-37 1.0486817539819029 2305 38-39 1.0578553466423628 2305 40-41 1.0522190198847596 2305 42-43 1.049666636030615 2305 44-45 1.0520636365185538 2305 46-47 1.0391533880302362 2305 48-49 1.0901238787571899 2305 50-51 1.0083386534180363 2305 52-53 1.0517262864571393 2305 54-55 1.1295218389460118 2305 56-57 1.1691336444575953 2305 58-59 1.167143168640166 2305 60-61 1.1761533841284688 2305 62-63 1.1532735969682264 2305 64-65 1.155814158361494 2305 66-67 1.1489731321075496 2305 68-69 1.133255354278326 2305 70-71 1.137390033491961 2305 72-73 1.163574608706476 2305 74-75 1.1513619916219113 2305 76-77 1.0918284461870869 2305 78-79 1.065140831431755 2305 80-81 1.0828967268752834 2305 82-83 1.0669433986782124 2305 84-85 1.0805911710611653 2305 86-87 1.1196373435232019 2305 88-89 1.07300097497793 2305 90-91 1.1228124729980777 2305 92-93 1.0931534526291742 2306 1 0.1872791918718022 2306 2 0.26386065504884115 2306 3 0.33092016308776806 2306 4 0.29070175949513555 2306 5 0.23853632156602345 2306 6 0.29919372805179023 2306 7 0.3766201649321914 2306 8 0.4612783308594004 2306 9 0.5490045130536743 2306 10-11 0.5666227492787712 2306 12-13 0.5982098288730384 2306 14-15 0.9249166561059639 2306 16-17 0.9941622979075078 2306 18-19 0.9482239029066974 2306 20-21 0.9211363250652553 2306 22-23 0.9647843075757336 2306 24-25 0.9350261084842089 2306 26-27 0.9762049003068185 2306 28-29 0.9609200872877608 2306 30-31 0.995930571491698 2306 32-33 0.9646023224954874 2306 34-35 1.0053280260963433 2306 36-37 1.01687363250916 2306 38-39 1.0566518488852878 2306 40-41 1.0848042810321843 2306 42-43 1.0736758370994153 2306 44-45 1.06005204298026 2306 46-47 1.0701265673088898 2306 48-49 1.103567794470706 2306 50-51 1.0347166749599594 2306 52-53 1.0773573067667215 2306 54-55 1.206320703816239 2306 56-57 1.1882433528393648 2306 58-59 1.221081606653648 2306 60-61 1.2006180712464527 2306 62-63 1.112858595228495 2306 64-65 1.0742752715649644 2306 66-67 1.1136780110557396 2306 68-69 1.1336933189756806 2306 70-71 1.1616574358633684 2306 72-73 1.181443979561415 2306 74-75 1.1209343760960735 2306 76-77 1.1298078649169057 2306 78-79 1.1241982884581674 2306 80-81 1.1370275506761018 2306 82-83 1.0846231321093072 2306 84-85 1.1185579314810141 2306 86-87 1.108652102785605 2306 88-89 1.1124525002550882 2306 90-91 1.1424419733882445 2306 92-93 1.0918639951812992 2307 1 0.1559196481091405 2307 2 0.20079037327755955 2307 3 0.25870570544308436 2307 4 0.21281383275789523 2307 5 0.2239122668086253 2307 6 0.21577041488809812 2307 7 0.22728668844737854 2307 8 0.28427298815048374 2307 9 0.39589760698698484 2307 10-11 0.2741769212042158 2307 12-13 0.31993860553032505 2307 14-15 0.524465796638566 2307 16-17 0.518024232026562 2307 18-19 0.4900894144393533 2307 20-21 0.503651320330782 2307 22-23 0.5393512347428526 2307 24-25 0.5319252962858769 2307 26-27 0.5224831476978764 2307 28-29 0.5163819569058461 2307 30-31 0.5037319924552719 2307 32-33 0.5108000236733119 2307 34-35 0.5242692134655869 2307 36-37 0.536861433274403 2307 38-39 0.5196652015519732 2307 40-41 0.5128924743898793 2307 42-43 0.5759611287203938 2307 44-45 0.5817332393678143 2307 46-47 0.5836447487932119 2307 48-49 0.5554890281161065 2307 50-51 0.4936768414120749 2307 52-53 0.5555819480632849 2307 54-55 0.6793351842124764 2307 56-57 0.6700882587179109 2307 58-59 0.6688428222116087 2307 60-61 0.5695642455367818 2307 62-63 0.6262913125479272 2307 64-65 0.6227432322035611 2307 66-67 0.5856776754592374 2307 68-69 0.5548733243699289 2307 70-71 0.5692510022422539 2307 72-73 0.6193298919736421 2307 74-75 0.5927663644780523 2307 76-77 0.5506917850396107 2307 78-79 0.5175744907563811 2307 80-81 0.5002260488838175 2307 82-83 0.4848805645391465 2307 84-85 0.44874659496993985 2307 86-87 0.5034708452555918 2307 88-89 0.5118585696705011 2307 90-91 0.5435355675180915 2307 92-93 0.4660270476492556 2308 1 0.29044513224119584 2308 2 0.2906542271210988 2308 3 0.33387136698933517 2308 4 0.37269802962833865 2308 5 0.33909524361419585 2308 6 0.38336816748575586 2308 7 0.410153009469056 2308 8 0.3937516803029695 2308 9 0.4882271361543573 2308 10-11 0.46604290062133913 2308 12-13 0.4489648931400154 2308 14-15 0.6854598998219075 2308 16-17 0.6314193325445245 2308 18-19 0.6022815824702548 2308 20-21 0.5780058689417871 2308 22-23 0.6226669266807292 2308 24-25 0.5759379625353986 2308 26-27 0.655383493209925 2308 28-29 0.6619364239174672 2308 30-31 0.6532734519879142 2308 32-33 0.694409926961562 2308 34-35 0.6682440805043015 2308 36-37 0.6784420076631292 2308 38-39 0.6997807550750963 2308 40-41 0.6814736921252234 2308 42-43 0.6306256426988952 2308 44-45 0.6756744477124457 2308 46-47 0.6828519746884965 2308 48-49 0.6655292291878254 2308 50-51 0.6321625834066182 2308 52-53 0.7086530298835854 2308 54-55 0.8286464626901662 2308 56-57 0.8669356547326004 2308 58-59 0.8546012542095625 2308 60-61 0.8305030221231817 2308 62-63 0.7915511492769909 2308 64-65 0.8211348694443572 2308 66-67 0.7745620849342743 2308 68-69 0.7939922034324454 2308 70-71 0.7480129923441368 2308 72-73 0.8072191365380021 2308 74-75 0.8426600553868226 2308 76-77 0.8115013180864636 2308 78-79 0.7921455017329393 2308 80-81 0.7779591548880447 2308 82-83 0.7454906831935872 2308 84-85 0.7180986511437837 2308 86-87 0.7325662500907484 2308 88-89 0.6918831318216689 2308 90-91 0.7750327082266963 2308 92-93 0.7226416535863578 2309 1 0.13030531122041822 2309 2 0.15798845905205283 2309 3 0.17284216480835823 2309 4 0.18985845285699554 2309 5 0.2075438988416991 2309 6 0.2322131148059654 2309 7 0.24755544276011676 2309 8 0.1998835347845329 2309 9 0.25069358793804497 2309 10-11 0.29538107857694484 2309 12-13 0.2569850143190493 2309 14-15 0.41159538847184507 2309 16-17 0.3887534207834875 2309 18-19 0.3160407005409027 2309 20-21 0.2552905831046175 2309 22-23 0.3560729389451893 2309 24-25 0.32069706479086335 2309 26-27 0.25080066519934974 2309 28-29 0.24769821062139386 2309 30-31 0.2671066595781184 2309 32-33 0.24250216175369133 2309 34-35 0.2161473759875392 2309 36-37 0.2970468642963269 2309 38-39 0.2910358747602899 2309 40-41 0.27181329407501664 2309 42-43 0.24545641858108525 2309 44-45 0.20593648021502275 2309 46-47 0.18725111389088056 2309 48-49 0.19388367596307177 2309 50-51 0.16415731533391664 2309 52-53 0.22839173703697924 2309 54-55 0.2975457419763856 2309 56-57 0.36258398202367204 2309 58-59 0.3439274165024315 2309 60-61 0.2821455895358014 2309 62-63 0.2278674631106412 2309 64-65 0.30328173467400177 2309 66-67 0.18764854753203508 2309 68-69 0.22425678471837074 2309 70-71 0.35998000282005904 2309 72-73 0.4073500504909262 2309 74-75 0.29907477525910764 2309 76-77 0.3077667572930025 2309 78-79 0.28797711840595497 2309 80-81 0.27370112010262204 2309 82-83 0.2983505380371696 2309 84-85 0.2552945700938949 2309 86-87 0.2759135374929329 2309 88-89 0.20901670628884972 2309 90-91 0.33106699711288456 2309 92-93 0.272747689514766 2310 1 0.2934296425609375 2310 2 0.2647200402106584 2310 3 0.273760934866786 2310 4 0.36289049052497546 2310 5 0.4032930771702823 2310 6 0.3950061112167518 2310 7 0.38690622347717607 2310 8 0.40522435653137734 2310 9 0.44395013562841257 2310 10-11 0.4169992030428986 2310 12-13 0.3884777075926351 2310 14-15 0.5774027706239266 2310 16-17 0.5769325046047342 2310 18-19 0.5644906542582504 2310 20-21 0.4876658764947521 2310 22-23 0.5467777892684182 2310 24-25 0.5760205959705189 2310 26-27 0.5815027560071542 2310 28-29 0.5992557618152929 2310 30-31 0.5978498801870344 2310 32-33 0.580720676919853 2310 34-35 0.5882611662760837 2310 36-37 0.6039995926902364 2310 38-39 0.6022574981431319 2310 40-41 0.5900130198271967 2310 42-43 0.6087105906675845 2310 44-45 0.5811056510334183 2310 46-47 0.6306419583255121 2310 48-49 0.6456364678537838 2310 50-51 0.5980447660124284 2310 52-53 0.6178828322754697 2310 54-55 0.7219645996704216 2310 56-57 0.7446876485966953 2310 58-59 0.7422619026366988 2310 60-61 0.7595740535964381 2310 62-63 0.7041252846895247 2310 64-65 0.6505210934284236 2310 66-67 0.702676766686551 2310 68-69 0.689639693385935 2310 70-71 0.7157986069001865 2310 72-73 0.7472056043464974 2310 74-75 0.7090692732264259 2310 76-77 0.7030526725768169 2310 78-79 0.7186915399304468 2310 80-81 0.6640266420018719 2310 82-83 0.6764213253386302 2310 84-85 0.6815265561008559 2310 86-87 0.706227833491333 2310 88-89 0.6790354433015793 2310 90-91 0.6773187741108302 2310 92-93 0.7006322151167339 2311 1 0.21463165913026216 2311 2 0.16658610543285945 2311 3 0.19284338296515813 2311 4 0.28855401098628164 2311 5 0.345851819729198 2311 6 0.3072183055995623 2311 7 0.2595100731519224 2311 8 0.19702719455924012 2311 9 0.3565954103893674 2311 10-11 0.33330694511996484 2311 12-13 0.30482569841674234 2311 14-15 0.5309105653294992 2311 16-17 0.47473986318323824 2311 18-19 0.43570093172941426 2311 20-21 0.45103060767159775 2311 22-23 0.5026656132628275 2311 24-25 0.4775624224947421 2311 26-27 0.4002259398231658 2311 28-29 0.38458339466343716 2311 30-31 0.4321950751884813 2311 32-33 0.38773503520521047 2311 34-35 0.39466201304595216 2311 36-37 0.4441618248822934 2311 38-39 0.43932132384621525 2311 40-41 0.44725870179689764 2311 42-43 0.4648567040418641 2311 44-45 0.4662175798689887 2311 46-47 0.42698758113482427 2311 48-49 0.4510425896857768 2311 50-51 0.41330767166443394 2311 52-53 0.4585913075425978 2311 54-55 0.5502847099610406 2311 56-57 0.47606836144097997 2311 58-59 0.4460394664608529 2311 60-61 0.45607995507278076 2311 62-63 0.44946963223436853 2311 64-65 0.4193716199082189 2311 66-67 0.4128851059210419 2311 68-69 0.391501586424603 2311 70-71 0.475884559497338 2311 72-73 0.46279298030963645 2311 74-75 0.45485772196933283 2311 76-77 0.47883391246271145 2311 78-79 0.38601207783424485 2311 80-81 0.2853808098975108 2311 82-83 0.3049706740120115 2311 84-85 0.26294795549291905 2311 86-87 0.31888865620663864 2311 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-1.0205562478317134 2303 16-17 -1.2892635609945913 2303 18-19 -1.288072385253237 2303 20-21 -0.9481277856822246 2303 22-23 -0.8511342633820362 2303 24-25 -0.9061831130588445 2303 26-27 -0.7707840327570139 2303 28-29 -0.858223499780145 2303 30-31 -0.8335581564683707 2303 32-33 -0.8685971303826037 2303 34-35 -0.8995796874244277 2303 36-37 -0.8901498966325434 2303 38-39 -1.100527289585152 2303 40-41 -0.9722088497843515 2303 42-43 -1.091763829545343 2303 44-45 -0.8793597287412567 2303 46-47 -0.8422820390761174 2303 48-49 -0.7536749539152439 2303 50-51 -0.953700439923999 2303 52-53 -0.9997993635699416 2303 54-55 -1.164191547718623 2303 56-57 -1.4174584007507534 2303 58-59 -1.150030152149462 2303 60-61 -1.3727847182599646 2303 62-63 -1.110967165202652 2303 64-65 -1.1121877606317554 2303 66-67 -1.1396405336071709 2303 68-69 -0.8931570812669136 2303 70-71 -0.9717777026961443 2303 72-73 -1.8869431044661553 2303 74-75 -1.3791759888470025 2303 76-77 -1.3589456307216778 2303 78-79 -1.337395727842562 2303 80-81 -1.7056118920096068 2303 82-83 -1.2596970445929827 2303 84-85 -1.0519479780003778 2303 86-87 -1.0465246926131755 2303 88-89 -0.8730207549618783 2303 90-91 -0.698731283426568 2303 92-93 -0.9913631398793754 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 310841.0 3 15851.0 4 9995.0 5 6934.0 6 7186.0 7 18847.0 8 37363.0 9 44357.0 10 54459.0 11 87139.0 12 141136.0 13 190928.0 14 221359.0 15 240693.0 16 259079.0 17 281061.0 18 305911.0 19 334965.0 20 369198.0 21 408673.0 22 453947.0 23 509481.0 24 578693.0 25 661615.0 26 767248.0 27 894267.0 28 1060136.0 29 1269465.0 30 1570340.0 31 1987143.0 32 2597308.0 33 3512376.0 34 4963464.0 35 7520350.0 36 1.2952804E7 37 2.7054657E7 38 3.7223921E7 39 1686991.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.64168953278136 23.601301889827884 11.217395168171123 24.539613409219623 2 15.639536384353262 22.635216522825978 38.8348425611938 22.89040453162696 3 15.347927767589534 27.52328083260854 31.826711099056844 25.302080300745082 4 11.486423240510275 17.26836334131403 42.485697139395185 28.759516278780506 5 11.97109745121094 38.34411060350591 38.0725328947234 11.612259050559754 6 28.245626736327523 41.85612108128195 18.617117134280956 11.281135048109576 7 25.690751214721818 32.77533301315327 26.490144844696946 15.043770927427966 8 22.91626769121778 37.539389593009936 23.228391609135123 16.315951106637165 9 23.562139142182417 15.290936100179287 25.005523240218196 36.1414015174201 10-11 22.477669718775388 27.108868222431887 31.65062391059305 18.76283814819967 12-13 21.858266109334714 25.510271378459443 31.70915175860282 20.92231075360303 14-15 19.975187479507515 26.975491920185394 27.220601779175603 25.828718821131485 16-17 20.0525044147683 31.45292252042245 28.408870305085525 20.085702759723723 18-19 20.079881388170364 30.209931587630145 29.939026145432766 19.77116087876672 20-21 20.378783717486566 29.79437875701234 29.703593952821254 20.123243572679836 22-23 20.55327427467711 29.60191320518296 29.613638730356545 20.231173789783384 24-25 20.03728732514531 29.525588112591617 29.932955640978463 20.504168921284606 26-27 20.32145687846224 30.06546804849074 29.62040020680575 19.992674866241273 28-29 20.27064714236401 29.856113029433722 29.71053206508002 20.16270776312225 30-31 20.121830943649602 29.735263564568854 29.872374131659384 20.270531360122167 32-33 20.31845353359827 29.989361651797257 29.69855990365371 19.993624910950764 34-35 20.201827271015343 29.69637719830367 29.803461877597915 20.298333653083077 36-37 20.159964504652503 29.88124853807383 29.84610880067334 20.11267815660033 38-39 20.187157949080646 29.835677527394434 29.86664841727008 20.11051610625484 40-41 20.070613196878988 29.768770970978554 30.028620184855566 20.13199564728689 42-43 20.12027896684018 29.784830638657812 29.96855890679982 20.12633148770219 44-45 20.038803716369326 29.69131942326223 29.985985680098466 20.28389118026998 46-47 19.997872360932128 29.71107128622721 30.114155705216987 20.17690064762367 48-49 20.03131369852055 29.781329195916186 30.04193585051261 20.14542125505066 50-51 19.89675279309109 29.76909135428782 30.16342047852606 20.170735374095035 52-53 19.976517852831645 29.666231446148466 30.230579250517508 20.12667145050238 54-55 19.99845580228139 29.754976885060596 30.11027581737166 20.13629149528635 56-57 19.922179446934585 29.806082149300728 30.036434625755337 20.235303778009346 58-59 19.95108773302793 29.779446854825274 30.03323854678478 20.23622686536201 60-61 19.85770777083083 29.686096182973216 30.175072248054718 20.28112379814123 62-63 19.806951009102256 29.76966043739347 30.119108621364415 20.30427993213986 64-65 19.91849185178916 29.638448469281897 30.133404040645623 20.30965563828332 66-67 19.86911285878435 29.592560856874194 30.10340738215674 20.434918902184716 68-69 19.925752529506344 29.716389983797963 29.960789302352666 20.397068184343027 70-71 20.04188212755134 29.57461882673374 29.956540592343313 20.426958453371608 72-73 20.012397869650204 29.631158499713923 29.899668285175323 20.45677534546055 74-75 19.941573448738875 29.660555146396504 29.94926957168081 20.44860183318381 76-77 19.965049202343273 29.624882142524495 29.9477544306067 20.462314224525528 78-79 20.147433322449505 29.619580864478586 29.81896791144521 20.414017901626703 80-81 20.047639260510476 29.684970523749644 29.788374270330976 20.47901594540891 82-83 20.12366035163963 29.66538486080175 29.851743513837764 20.359211273720856 84-85 20.066224683878385 29.68267059372907 29.878967112462636 20.37213760992991 86-87 19.989538437856833 29.56842618588044 29.949947599159298 20.492087777103425 88-89 20.141763314736682 29.538053498917076 29.877531567631443 20.442651618714798 90-91 20.26615987276835 29.62231294435528 29.763782797674793 20.347744385201587 92-93 20.33507764524495 29.453577187253323 29.783979860341127 20.4273653071606 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 292038.0 1 152688.0 2 12084.5 3 9795.0 4 8072.5 5 7125.0 6 6935.5 7 7267.5 8 7982.5 9 9374.5 10 11472.5 11 14478.5 12 19501.0 13 27585.5 14 32493.0 15 39669.5 16 58548.5 17 88304.5 18 136233.5 19 205264.5 20 305764.5 21 442473.5 22 621106.5 23 852469.0 24 1123972.0 25 1442997.0 26 1831317.0 27 2271833.0 28 2731634.5 29 2963624.0 30 3176184.0 31 3588824.5 32 3962773.0 33 4286418.0 34 4563514.0 35 4799426.0 36 4936741.5 37 4969304.0 38 4936928.5 39 4838576.0 40 4711245.5 41 4540421.5 42 4331955.5 43 4229120.0 44 4097971.5 45 3794234.0 46 3487308.5 47 3200263.5 48 2906129.0 49 2627187.5 50 2354763.5 51 2116655.5 52 1893598.0 53 1679490.5 54 1497354.5 55 1332589.5 56 1172055.0 57 1088399.0 58 1023595.0 59 900750.5 60 791767.0 61 692391.5 62 605098.0 63 527470.0 64 462538.5 65 420318.5 66 385502.0 67 341658.0 68 289141.0 69 244346.0 70 208180.5 71 189376.0 72 176795.5 73 152072.5 74 129689.5 75 113883.0 76 106460.5 77 103482.0 78 95665.5 79 87458.5 80 79398.0 81 70221.5 82 62825.0 83 51790.0 84 35375.5 85 20806.0 86 16384.0 87 13464.5 88 8463.5 89 5181.0 90 3334.5 91 2257.5 92 1537.5 93 1069.5 94 731.5 95 526.5 96 397.5 97 272.5 98 196.0 99 134.5 100 107.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.46490205092422726 2 0.45344740914943443 3 0.4526861772335406 4 0.4497036308077283 5 0.4519403146081101 6 0.3478169880221062 7 0.3666253832456888 8 0.3434792318077845 9 0.3399099401166336 10-11 0.3359451152150271 12-13 0.30624260528061154 14-15 0.2932166795749118 16-17 0.29070606077391736 18-19 0.2960129863633439 20-21 0.2999845918342725 22-23 0.32093926326727557 24-25 0.32678501809883126 26-27 0.3288395306034261 28-29 0.3324702994564307 30-31 0.33974178199744565 32-33 0.34457271162046105 34-35 0.3347987469616382 36-37 0.3154953701775427 38-39 0.3122208072329255 40-41 0.3265906417782645 42-43 0.31673214602189287 44-45 0.31490726879833963 46-47 0.33506725750679317 48-49 0.3526334524305679 50-51 0.37225506393484703 52-53 0.40019598195938216 54-55 0.41801667425171285 56-57 0.4353690552228642 58-59 0.4281676385648442 60-61 0.39036370440438933 62-63 0.3754654374898817 64-65 0.356503349361665 66-67 0.35337976709395313 68-69 0.346253388736431 70-71 0.3371701380725523 72-73 0.3850716960674714 74-75 0.3840794727566715 76-77 0.3963889183040031 78-79 0.43296602145511365 80-81 0.45426966618922715 82-83 0.4514801399701172 84-85 0.43704747214906015 86-87 0.3893411945506174 88-89 0.3472212019976714 90-91 0.3416159313580727 92-93 0.3247955990597285 >>END_MODULE >>Sequence Length Distribution pass #Length Count 93 1.10610181E8 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.05808559872576 #Duplication Level Percentage of deduplicated Percentage of total 1 81.46395929138627 46.48177562459029 2 11.376600915293562 12.98254137694724 3 3.261992141893588 5.583690805636055 4 1.3513421314264265 3.0841998003237427 5 0.579790463323567 1.654086694282048 6 0.381454974415721 1.3059054349363173 7 0.20864010836903185 0.8333223613853348 8 0.20436575123644213 0.9328574821997446 9 0.10657683262019538 0.5472963034635758 >10 0.8719561434431206 9.906891224628085 >50 0.10990290593085618 4.426086701313808 >100 0.07673314995992746 8.667836831836787 >500 0.005229095765558164 1.9626651579984489 >1k 0.001443466214987144 1.2579587380419692 >5k 0.0 0.0 >10k+ 1.2628720834563016E-5 0.37288546241657555 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 280461 0.2535580336858865 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 2.7122277288380894E-6 0.0 2 1.808151819225393E-6 3.616303638450786E-6 2.7122277288380894E-6 3 1.808151819225393E-6 7.232607276901572E-6 8.136683186514268E-6 4 4.5203795480634825E-6 9.944835005739662E-6 1.808151819225393E-5 5 9.040759096126965E-6 1.0848910915352358E-5 1.808151819225393E-5 6 2.350597364993011E-5 1.1752986824965055E-5 1.8985594101866625E-5 7 2.4410049559542805E-5 1.4465214553803145E-5 2.4410049559542805E-5 8 3.616303638450786E-5 1.536929046341584E-5 2.4410049559542805E-5 9 4.339564366140943E-5 1.7177442282641234E-5 4.610787139024752E-5 10-11 5.5600668441180834E-5 2.53141254691555E-5 9.131166687088235E-5 12-13 6.870976913056494E-5 3.164265683644437E-5 1.0758503324391087E-4 14-15 8.091479391033633E-5 3.5710998429701514E-5 1.3561138644190446E-4 16-17 9.628408437375218E-5 4.339564366140944E-5 1.844314855609901E-4 18-19 1.1979005802368228E-4 4.7463985254666565E-5 2.3777196422813918E-4 20-21 1.3244712075826005E-4 5.108028889311735E-5 3.0738580926831683E-4 22-23 1.4691233531206318E-4 5.514863048637449E-5 4.7147558686302124E-4 24-25 1.6092551191105998E-4 5.740882026040623E-5 0.001043303599693052 26-27 1.7493868851005677E-4 6.328531367288876E-5 0.0028876184553029522 28-29 1.889518651090536E-4 6.64495793565332E-5 0.013154756522819541 30-31 2.0386911761766308E-4 6.780569322095223E-5 0.023885685531967443 32-33 2.1878637012627257E-4 7.142199685940303E-5 0.03622677373613556 34-35 2.3279954672526934E-4 7.684645231707921E-5 0.060797296769634614 36-37 2.51785140827136E-4 8.046275595552999E-5 0.1219060476901308 38-39 2.6670239333574544E-4 8.362702163917444E-5 0.2310388588912986 40-41 2.770992662962915E-4 8.859943914204426E-5 0.3579901021950231 42-43 2.9066040494048196E-4 9.131166687088235E-5 0.49410099057698853 44-45 3.0376950562986606E-4 9.131166687088235E-5 0.659250345137759 46-47 3.2049490995770093E-4 9.221574278049505E-5 0.8591261594626629 48-49 3.3541216246631036E-4 9.718816028336487E-5 1.0603635121074433 50-51 3.5982221202585324E-4 1.0170853983142836E-4 1.2594093847473227 52-53 3.8152003385655796E-4 1.0577688142468549E-4 1.4546368927829527 54-55 4.0864231114493883E-4 1.0984522301794262E-4 1.661673440349944 56-57 4.312442088852562E-4 1.152696784756188E-4 1.8956559704029414 58-59 4.5520222048999273E-4 1.179819062044569E-4 2.1341272373471663 60-61 5.15323268479237E-4 1.2521451348135846E-4 2.365646612584424 62-63 5.261721793945893E-4 1.292828550746156E-4 2.5857683028291945 64-65 5.383772041743608E-4 1.3244712075826005E-4 2.8067701109719727 66-67 5.501301909993258E-4 1.378715762159362E-4 3.045261267586209 68-69 5.600750260050654E-4 1.405838039447743E-4 3.2915432983515327 70-71 5.853891514742209E-4 1.451041834928378E-4 3.53164190193306 72-73 6.084430871693447E-4 1.478164112216759E-4 3.7627991947685175 74-75 6.25620529451986E-4 1.4917252508609493E-4 3.9870262937188397 76-77 6.387296301413701E-4 1.5052863895051395E-4 4.221703606108374 78-79 6.527428067403669E-4 1.5324086667935205E-4 4.465585315333676 80-81 6.631396797009128E-4 1.6228162577547903E-4 4.709366220095056 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACTG 47415 0.0 15.82435 5 CTATACT 57030 0.0 14.805284 4 TATACAG 46845 0.0 14.279567 5 GTATATA 92615 0.0 13.640123 1 GTATAAG 49930 0.0 13.600824 1 GTATTAG 50070 0.0 13.545408 1 TATACCG 19390 0.0 13.422325 5 TCTATAC 59220 0.0 13.266284 3 TACACTG 31985 0.0 13.157837 5 GTATAGA 57670 0.0 12.945427 1 ACTGCGC 10435 0.0 12.750474 8 GTATAGG 41755 0.0 12.593912 1 GTGTAGG 37870 0.0 12.322581 1 GTATTAT 78710 0.0 12.322178 1 TACACCG 18435 0.0 12.158177 5 TTATACT 78780 0.0 12.121016 4 CTTACAC 32810 0.0 12.085166 3 CAGCGCG 11020 0.0 12.073009 9 GTGTAAG 33490 0.0 12.023441 1 GTATAAT 71180 0.0 11.791017 1 >>END_MODULE