FastQCFastQC Report
Wed 5 Nov 2014
lane5_Undetermined_L005_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane5_Undetermined_L005_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110610181
Sequences flagged as poor quality0
Sequence length93
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2804610.2535580336858865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG474150.015.824355
CTATACT570300.014.8052844
TATACAG468450.014.2795675
GTATATA926150.013.6401231
GTATAAG499300.013.6008241
GTATTAG500700.013.5454081
TATACCG193900.013.4223255
TCTATAC592200.013.2662843
TACACTG319850.013.1578375
GTATAGA576700.012.9454271
ACTGCGC104350.012.7504748
GTATAGG417550.012.5939121
GTGTAGG378700.012.3225811
GTATTAT787100.012.3221781
TACACCG184350.012.1581775
TTATACT787800.012.1210164
CTTACAC328100.012.0851663
CAGCGCG110200.012.0730099
GTGTAAG334900.012.0234411
GTATAAT711800.011.7910171
Produced by FastQC (version 0.11.2)