##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename lane5_Undetermined_L005_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 110610181 Sequences flagged as poor quality 0 Sequence length 93 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.53414677985203 33.0 33.0 33.0 33.0 33.0 2 32.61306383722489 33.0 33.0 33.0 33.0 33.0 3 32.67496505588396 33.0 33.0 33.0 33.0 33.0 4 36.625265571168356 37.0 37.0 37.0 37.0 37.0 5 36.504545191911404 37.0 37.0 37.0 37.0 37.0 6 36.507962047363435 37.0 37.0 37.0 37.0 37.0 7 36.48959839420207 37.0 37.0 37.0 37.0 37.0 8 36.49262169636989 37.0 37.0 37.0 37.0 37.0 9 36.49501595156055 37.0 37.0 37.0 37.0 37.0 10-11 36.453344950226594 37.0 37.0 37.0 37.0 37.0 12-13 36.38689570537815 37.0 37.0 37.0 37.0 37.0 14-15 38.39589491314547 40.0 37.0 40.0 37.0 40.0 16-17 38.43851040710258 40.0 37.0 40.0 37.0 40.0 18-19 38.480823668483104 40.0 37.0 40.0 37.0 40.0 20-21 38.49291263251798 40.0 37.0 40.0 37.0 40.0 22-23 38.44238689474706 40.0 37.0 40.0 37.0 40.0 24-25 38.37715846880316 40.0 37.0 40.0 37.0 40.0 26-27 38.25987753333484 40.0 37.0 40.0 37.0 40.0 28-29 38.07113732143698 40.0 37.0 40.0 35.0 40.0 30-31 38.066311115610596 40.0 37.0 40.0 37.0 40.0 32-33 38.031958323981044 40.0 37.0 40.0 37.0 40.0 34-35 38.00117096815889 40.0 37.0 40.0 33.0 40.0 36-37 37.8699227560255 40.0 37.0 40.0 33.0 40.0 38-39 37.75084895214121 40.0 37.0 40.0 33.0 40.0 40-41 37.666588815183296 40.0 37.0 40.0 33.0 40.0 42-43 37.624239594183464 40.0 37.0 40.0 33.0 40.0 44-45 37.514302494451215 40.0 37.0 40.0 33.0 40.0 46-47 37.39301340624332 40.0 37.0 40.0 33.0 40.0 48-49 37.24359207946689 40.0 37.0 40.0 33.0 40.0 50-51 37.05904937448751 40.0 37.0 40.0 33.0 40.0 52-53 37.09162680965146 40.0 37.0 40.0 33.0 40.0 54-55 36.96779857452724 37.0 37.0 40.0 33.0 40.0 56-57 36.75837227406761 37.0 37.0 40.0 33.0 40.0 58-59 36.6202266001174 37.0 37.0 40.0 33.0 40.0 60-61 36.41982749309487 37.0 37.0 40.0 33.0 40.0 62-63 36.2195106388986 37.0 37.0 40.0 33.0 40.0 64-65 35.97967321832698 37.0 37.0 40.0 33.0 40.0 66-67 35.747387561909875 37.0 35.0 40.0 33.0 40.0 68-69 35.5176195218413 37.0 33.0 40.0 30.0 40.0 70-71 35.304661494044566 37.0 33.0 37.0 27.0 40.0 72-73 35.013933794213756 37.0 33.0 37.0 27.0 40.0 74-75 34.67814766978819 37.0 33.0 37.0 27.0 40.0 76-77 33.27431393950978 35.0 33.0 37.0 27.0 37.0 78-79 34.29784095100612 37.0 33.0 37.0 27.0 37.0 80-81 34.379026511131016 37.0 33.0 37.0 27.0 37.0 82-83 34.226140833274656 37.0 33.0 37.0 27.0 37.0 84-85 34.10255799599496 37.0 33.0 37.0 27.0 37.0 86-87 33.94300734848269 37.0 33.0 37.0 27.0 37.0 88-89 33.78795471368047 37.0 33.0 37.0 27.0 37.0 90-91 33.64974586290569 37.0 33.0 37.0 27.0 37.0 92-93 32.58845063728808 35.0 33.0 37.0 24.5 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2304 1 0.08158224050095697 2304 2 0.11857022814568552 2304 3 0.0953648765145445 2304 4 -0.05750386936955465 2304 5 -0.11971661350537488 2304 6 -0.08997152408350928 2304 7 -0.05046381659145993 2304 8 -0.03585739637055241 2304 9 -0.0029604231902027323 2304 10-11 -0.017369969443372213 2304 12-13 0.12018525300199201 2304 14-15 0.3650781710486015 2304 16-17 0.398391702615136 2304 18-19 0.3122423249930151 2304 20-21 0.23773135861105743 2304 22-23 0.26780065658374497 2304 24-25 0.2643450980669897 2304 26-27 0.1323493847352637 2304 28-29 0.0025687296810659177 2304 30-31 -0.1114494487931168 2304 32-33 -0.22456294650123 2304 34-35 -0.33116907994097033 2304 36-37 -0.17435507693393504 2304 38-39 -0.24054953403262402 2304 40-41 -0.32587404115174223 2304 42-43 -0.3572656838662027 2304 44-45 -0.47826308229304715 2304 46-47 -0.45590476089478216 2304 48-49 -0.4529460766997673 2304 50-51 -0.4248977662998641 2304 52-53 -0.588369946212616 2304 54-55 -0.7290424611120585 2304 56-57 -0.6897881683429716 2304 58-59 -0.6251927191493181 2304 60-61 -0.6917365078977085 2304 62-63 -0.8140792901252922 2304 64-65 -0.8236098318873388 2304 66-67 -0.8305141965916505 2304 68-69 -0.9378276538777541 2304 70-71 -0.7975334312320328 2304 72-73 -0.8160907370409092 2304 74-75 -0.9469126537813253 2304 76-77 -0.8070018271260793 2304 78-79 -0.5294208410095749 2304 80-81 -0.5346420614666485 2304 82-83 -0.5144758432033001 2304 84-85 -0.507306173405361 2304 86-87 -0.5635833072870184 2304 88-89 -0.5552251479013606 2304 90-91 -0.5597754744385455 2304 92-93 -0.6442642210116105 2305 1 0.17740049563651183 2305 2 0.16892176628576294 2305 3 0.16075798336428448 2305 4 0.1770995219629654 2305 5 0.12454753269002339 2305 6 0.19937189621228413 2305 7 0.21905534889238965 2305 8 0.231336334433486 2305 9 0.3707314604246079 2305 10-11 0.3419083531597593 2305 12-13 0.3991025016855545 2305 14-15 0.7456410542275833 2305 16-17 0.7054247744440758 2305 18-19 0.683856624370037 2305 20-21 0.601023961066879 2305 22-23 0.6152234077078162 2305 24-25 0.6168922451814751 2305 26-27 0.7014826370792449 2305 28-29 0.7714463035285561 2305 30-31 0.7498923801123283 2305 32-33 0.6519534207345075 2305 34-35 0.648889048794139 2305 36-37 0.8659443134344045 2305 38-39 0.7832028521205245 2305 40-41 0.7004585532797307 2305 42-43 0.6752815286783687 2305 44-45 0.6131222419106948 2305 46-47 0.6405649003887603 2305 48-49 0.640663879297847 2305 50-51 0.7648104279863759 2305 52-53 0.6557430708984668 2305 54-55 0.682435856243572 2305 56-57 0.7150520268715965 2305 58-59 0.7151872895803635 2305 60-61 0.6391644976382622 2305 62-63 0.6838175974378942 2305 64-65 0.6312831837201571 2305 66-67 0.5958888313553103 2305 68-69 0.5262442724903806 2305 70-71 0.567432311215164 2305 72-73 0.5747870218428517 2305 74-75 0.6259975530555835 2305 76-77 0.5773113667639791 2305 78-79 0.7176471973070662 2305 80-81 0.674286161149503 2305 82-83 0.6674742832435925 2305 84-85 0.7817334550407864 2305 86-87 0.7889998466534038 2305 88-89 0.7998706106527536 2305 90-91 0.7890991609441684 2305 92-93 0.7866900832111625 2306 1 0.18439828783364476 2306 2 0.1796178052613655 2306 3 0.14673152370835396 2306 4 0.17106068029679733 2306 5 0.13160071221950886 2306 6 0.17442417304845748 2306 7 0.16910636685869918 2306 8 0.20769626069810698 2306 9 0.3469064101437169 2306 10-11 0.3039164632324045 2306 12-13 0.3435921475222017 2306 14-15 0.6447998666515034 2306 16-17 0.6791217851585074 2306 18-19 0.6399400380305309 2306 20-21 0.578080595512823 2306 22-23 0.5808642676821165 2306 24-25 0.5892986829569082 2306 26-27 0.6856023923005878 2306 28-29 0.7640023494333903 2306 30-31 0.764197187640896 2306 32-33 0.6760080321434145 2306 34-35 0.5826912722340083 2306 36-37 0.811802914360463 2306 38-39 0.8041461865694259 2306 40-41 0.7205763841081918 2306 42-43 0.6696933175450681 2306 44-45 0.6659047889403595 2306 46-47 0.6822722020091945 2306 48-49 0.6134873190209618 2306 50-51 0.7313661434116625 2306 52-53 0.6885260508796236 2306 54-55 0.643631982338654 2306 56-57 0.7423287576351285 2306 58-59 0.8054345112622769 2306 60-61 0.780021601278321 2306 62-63 0.7596776556106235 2306 64-65 0.697417508641557 2306 66-67 0.6673801713571734 2306 68-69 0.6572034840483099 2306 70-71 0.7787702218412704 2306 72-73 0.6617252393503179 2306 74-75 0.6971794811003562 2306 76-77 0.7037206123424085 2306 78-79 0.8395463231312519 2306 80-81 0.742113374684088 2306 82-83 0.8149493979691158 2306 84-85 0.8673661903059369 2306 86-87 0.8350036547286734 2306 88-89 0.8154723632051244 2306 90-91 0.830685959592735 2306 92-93 0.8023187802958418 2307 1 0.169102840577807 2307 2 0.17839798794550177 2307 3 0.1669416113236224 2307 4 0.17051823664947818 2307 5 0.14479516509424428 2307 6 0.17225737759063975 2307 7 0.19955965462679615 2307 8 0.21286788093360087 2307 9 0.30792575578045245 2307 10-11 0.2794909885415606 2307 12-13 0.31324838963248425 2307 14-15 0.591657420625225 2307 16-17 0.5134955742127048 2307 18-19 0.48689810479175577 2307 20-21 0.4316597237289841 2307 22-23 0.4253679546750888 2307 24-25 0.4367096526608094 2307 26-27 0.5529379690099958 2307 28-29 0.6255990534303848 2307 30-31 0.5889818532557953 2307 32-33 0.5062773890328387 2307 34-35 0.49204735181159975 2307 36-37 0.667816829773237 2307 38-39 0.7059142476333378 2307 40-41 0.6164956103362869 2307 42-43 0.4763639056458757 2307 44-45 0.37098966920009957 2307 46-47 0.39507734399548866 2307 48-49 0.4620924225496097 2307 50-51 0.577814046603855 2307 52-53 0.4513254408275529 2307 54-55 0.40090899569148064 2307 56-57 0.5461024999001651 2307 58-59 0.6304980707293311 2307 60-61 0.5412053816716309 2307 62-63 0.5146412501327475 2307 64-65 0.43671391040722085 2307 66-67 0.45756745913595154 2307 68-69 0.40251857328180307 2307 70-71 0.4899338129049795 2307 72-73 0.44243863036430753 2307 74-75 0.32725263492547896 2307 76-77 0.3277407367178071 2307 78-79 0.5701925971273454 2307 80-81 0.5490988319529393 2307 82-83 0.5631974431477005 2307 84-85 0.5533540342088443 2307 86-87 0.5417330769234212 2307 88-89 0.46530047620085924 2307 90-91 0.5084545662259217 2307 92-93 0.4723885924028721 2308 1 0.19054585845279348 2308 2 0.177212679941249 2308 3 0.15125476397268756 2308 4 0.17451121896379362 2308 5 0.051966302560082056 2308 6 0.16193469532388605 2308 7 0.1780870580513536 2308 8 0.1849729070547781 2308 9 0.2711074032958649 2308 10-11 0.24909378281466132 2308 12-13 0.2898983451533539 2308 14-15 0.46989775421341307 2308 16-17 0.4946724737467534 2308 18-19 0.419315261080456 2308 20-21 0.330640418498632 2308 22-23 0.33398103242090826 2308 24-25 0.34686765629271576 2308 26-27 0.48571463898658607 2308 28-29 0.5689360536600319 2308 30-31 0.5364413677602542 2308 32-33 0.4647261734198267 2308 34-35 0.43856956378115086 2308 36-37 0.6608671930093308 2308 38-39 0.6523525691244032 2308 40-41 0.5191573270979291 2308 42-43 0.42163739822782986 2308 44-45 0.37681964437890514 2308 46-47 0.3797793821550428 2308 48-49 0.47277154825573575 2308 50-51 0.5827794562919948 2308 52-53 0.5211677598767679 2308 54-55 0.4881795660636925 2308 56-57 0.5535209650612316 2308 58-59 0.5803106679669412 2308 60-61 0.4613440475942667 2308 62-63 0.45167282729921254 2308 64-65 0.38253144507557124 2308 66-67 0.3597409825255511 2308 68-69 0.3530722361244827 2308 70-71 0.4186987201355663 2308 72-73 0.4107143738962691 2308 74-75 0.36964379258174773 2308 76-77 0.31921911036780415 2308 78-79 0.5242810366872916 2308 80-81 0.5434278019328076 2308 82-83 0.5468453965276865 2308 84-85 0.5799306806455675 2308 86-87 0.5605554806489366 2308 88-89 0.4397553761834203 2308 90-91 0.5493248092662739 2308 92-93 0.4954104100714005 2309 1 0.17071366587072845 2309 2 0.146152258463637 2309 3 0.12003666041517391 2309 4 0.10485177073010732 2309 5 -0.03205603394346923 2309 6 0.032270614627805116 2309 7 0.06388429412690044 2309 8 0.02203381707045793 2309 9 0.13500451617044718 2309 10-11 0.10394687057967644 2309 12-13 0.16212344630300635 2309 14-15 0.26093189952501916 2309 16-17 0.21655781992479461 2309 18-19 0.24300878511122193 2309 20-21 0.18185010525179734 2309 22-23 0.1781831717609279 2309 24-25 0.12479657507942932 2309 26-27 0.26935600096279444 2309 28-29 0.3722909675946724 2309 30-31 0.283053791376652 2309 32-33 0.21988537242585693 2309 34-35 0.14398284858894073 2309 36-37 0.3146307847057628 2309 38-39 0.2787911994215335 2309 40-41 0.19869932847679905 2309 42-43 0.16788226918368565 2309 44-45 0.11663380390309186 2309 46-47 0.16249016055138554 2309 48-49 0.12265764498297216 2309 50-51 0.21164097553385375 2309 52-53 0.15264761365425983 2309 54-55 0.0869634109483357 2309 56-57 0.2509241012069481 2309 58-59 0.27404462416924247 2309 60-61 0.22126825358127178 2309 62-63 0.24624842345348696 2309 64-65 0.17215336981607265 2309 66-67 0.15846840845266286 2309 68-69 -0.06279143302353418 2309 70-71 0.13640308914484223 2309 72-73 0.09613027222624737 2309 74-75 0.10120993905955089 2309 76-77 -0.050102781015688436 2309 78-79 0.17066142564952003 2309 80-81 0.2267212517766808 2309 82-83 0.2680604428949067 2309 84-85 0.184616597862707 2309 86-87 0.15374083819910567 2309 88-89 0.046288000751737 2309 90-91 0.16391251926973638 2309 92-93 0.07940012097031968 2310 1 0.17332852505707308 2310 2 0.1612461749539662 2310 3 0.1268666873945321 2310 4 0.15260860626723627 2310 5 0.13047051863315318 2310 6 0.1516096154271125 2310 7 0.15582191590068817 2310 8 0.17080677804901967 2310 9 0.2658618932676333 2310 10-11 0.2064627041060092 2310 12-13 0.27760751538041006 2310 14-15 0.46187971396538785 2310 16-17 0.3937387048480403 2310 18-19 0.3372964929848834 2310 20-21 0.28816448637208936 2310 22-23 0.327319519989679 2310 24-25 0.29602654021078934 2310 26-27 0.46072381840844656 2310 28-29 0.5322619206449772 2310 30-31 0.4755034710486754 2310 32-33 0.45328236382435705 2310 34-35 0.3855132728546167 2310 36-37 0.5637007680230681 2310 38-39 0.6175583520937522 2310 40-41 0.5196799249922464 2310 42-43 0.4062687254209152 2310 44-45 0.4351795403799912 2310 46-47 0.3969215369111012 2310 48-49 0.3956660838160744 2310 50-51 0.4774623512186338 2310 52-53 0.4636952449020555 2310 54-55 0.4846700995976221 2310 56-57 0.5373146380563085 2310 58-59 0.5545031773258771 2310 60-61 0.5253651323948816 2310 62-63 0.44232764454839213 2310 64-65 0.4447131672897626 2310 66-67 0.45258861227232217 2310 68-69 0.39396030243273117 2310 70-71 0.43567925918199535 2310 72-73 0.4465707294540451 2310 74-75 0.4126130594102406 2310 76-77 0.2938344721370427 2310 78-79 0.589675961095601 2310 80-81 0.543949974337913 2310 82-83 0.5679060044128903 2310 84-85 0.5485512762397278 2310 86-87 0.6062436537259899 2310 88-89 0.5877629943186662 2310 90-91 0.5140340441139486 2310 92-93 0.5621051340641259 2311 1 0.14927924180319252 2311 2 0.137294540818921 2311 3 0.12370492070115091 2311 4 0.12966665136972466 2311 5 0.13562343135851762 2311 6 0.14469206387455813 2311 7 0.134650787556005 2311 8 0.1246942498045911 2311 9 0.2135399620570837 2311 10-11 0.2281986610794391 2311 12-13 0.2290050704743507 2311 14-15 0.4002093156878672 2311 16-17 0.3348126069493489 2311 18-19 0.34180984425298533 2311 20-21 0.10695044552070243 2311 22-23 0.18031443135481595 2311 24-25 0.18609779605327503 2311 26-27 0.3518336456946827 2311 28-29 0.4391333253776395 2311 30-31 0.4361354443940897 2311 32-33 0.3901151846555777 2311 34-35 0.32324033262482743 2311 36-37 0.43897723743429395 2311 38-39 0.410334145296936 2311 40-41 0.40371603897189345 2311 42-43 0.2945040595402588 2311 44-45 0.2693777493459564 2311 46-47 0.27337664239882997 2311 48-49 0.33079862053038056 2311 50-51 0.414241685088669 2311 52-53 0.39210613002578754 2311 54-55 0.3821850660422541 2311 56-57 0.4677297036580583 2311 58-59 0.4662886718457102 2311 60-61 0.397005510432777 2311 62-63 0.4850574423102856 2311 64-65 0.4473231860922908 2311 66-67 0.3687205430980356 2311 68-69 0.1842533019773711 2311 70-71 0.30346706194895035 2311 72-73 0.36447171137880474 2311 74-75 0.3879760474604197 2311 76-77 0.2775468882223535 2311 78-79 0.46900191451074846 2311 80-81 0.454710059826958 2311 82-83 0.4338479407477891 2311 84-85 0.45785885865490883 2311 86-87 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-1.3886171761794017 2303 28-29 -1.0679434626876088 2303 30-31 -0.9412593310731197 2303 32-33 -0.9474483080770355 2303 34-35 -1.3874825585190322 2303 36-37 -1.2844715670009421 2303 38-39 -1.3566460047913225 2303 40-41 -1.108663046538183 2303 42-43 -1.167440351572985 2303 44-45 -1.299185870978313 2303 46-47 -1.1887948400318677 2303 48-49 -1.6719508734487007 2303 50-51 -1.6414305272357552 2303 52-53 -1.7484443825903995 2303 54-55 -1.923416988302904 2303 56-57 -1.8217973103028129 2303 58-59 -1.8622356000966036 2303 60-61 -1.9351591660076153 2303 62-63 -1.9926241583452722 2303 64-65 -2.006571146199917 2303 66-67 -1.9777795610990694 2303 68-69 -1.9191668852859323 2303 70-71 -3.238003148700656 2303 72-73 -2.231040655073272 2303 74-75 -1.8547958166365959 2303 76-77 -1.7058880109054222 2303 78-79 -1.8261789834361224 2303 80-81 -1.9523248796029762 2303 82-83 -1.798426632299666 2303 84-85 -1.754812150387032 2303 86-87 -1.7363425583404748 2303 88-89 -2.2180564765519577 2303 90-91 -1.9879645037497653 2303 92-93 -1.8565238823244101 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1.0 5 0.0 6 4.0 7 49.0 8 613.0 9 3823.0 10 18886.0 11 102138.0 12 74302.0 13 41103.0 14 24562.0 15 14415.0 16 18737.0 17 30662.0 18 49177.0 19 74600.0 20 110094.0 21 157493.0 22 227766.0 23 283844.0 24 370131.0 25 477986.0 26 608694.0 27 767375.0 28 970490.0 29 1229922.0 30 1569590.0 31 2035169.0 32 2696328.0 33 3693256.0 34 5338373.0 35 8406465.0 36 1.5155585E7 37 3.1229746E7 38 3.4171829E7 39 656973.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.63914922302942 24.17165984752763 11.505246530907346 24.6839443985356 2 15.613947869771591 23.05459115015823 38.30418377129317 23.02727720877701 3 15.254066892811613 27.646167580179622 31.812341939843673 25.287423587165094 4 11.567174815490086 17.65702381410984 41.90861056451937 28.8671908058807 5 11.624129789643867 38.578171208308575 38.270694087373386 11.527004914674174 6 28.218245117960706 42.166951159767116 18.436934842372242 11.177868879899943 7 25.64903315726425 32.862456847439745 26.450072439534296 15.038437555761707 8 22.900145150291365 37.7762739580003 23.058597110513723 16.26498378119461 9 23.478638914802968 15.184309299701807 24.929262162585196 36.40778962291003 10-11 22.456287274315194 27.219059970618797 31.59847103043797 18.726181724628045 12-13 21.881584752130546 25.577023963101553 31.665087863837776 20.876303420930125 14-15 19.945151341900434 27.097319368820127 27.12237266838936 25.835156620890075 16-17 20.00825945669504 31.614499844277443 28.336330540856814 20.040910158170703 18-19 20.0427865677211 30.317102997960017 29.906736614055447 19.733373820263438 20-21 20.33084031123171 29.881566318195418 29.689817154677346 20.097776215895525 22-23 20.455731125760924 29.708212247982342 29.599878013121906 20.23617861313483 24-25 20.02145323637421 29.61223591969783 29.90108513288513 20.465225711042827 26-27 20.285668273181393 30.15571470398829 29.598204585613065 19.960412437217254 28-29 20.28329288870357 29.961986104984923 29.659762517885486 20.094958488426016 30-31 20.107843221446277 29.79826112134608 29.858601586451982 20.23529407075566 32-33 20.30064987153307 30.04740960230689 29.679569834264242 19.972370691895797 34-35 20.17038793678476 29.74638076919473 29.800078612090637 20.283152681929874 36-37 20.151064094449595 29.955678566602472 29.816197713332375 20.077059625615554 38-39 20.138604462977845 29.914789337832676 29.863848028407645 20.08275817078184 40-41 20.050312883769863 29.8178816067888 30.01978653828922 20.112018971152118 42-43 20.064601514080515 29.863274839874016 29.960306282670164 20.11181736337531 44-45 20.022813983470375 29.736981194619005 29.97964794904257 20.260556872868047 46-47 19.96855285786687 29.75321233589905 30.113245142271044 20.164989663963038 48-49 19.9961435373478 29.84614824578276 30.020438953007726 20.137269263861715 50-51 19.922890446056606 29.82214062738074 30.125105746546627 20.12986318001603 52-53 19.947119491723864 29.7204336253006 30.208300606664423 20.124146276311112 54-55 19.918254647732855 29.79654246988745 30.120028943638893 20.165173938740807 56-57 19.844304578376764 29.84300784531223 30.02272375079535 20.289963825515652 58-59 19.874289655521483 29.825213502236785 30.012789836412125 20.287707005829606 60-61 19.779767259535504 29.732032329303937 30.14066710353966 20.347533307620903 62-63 19.71464258310014 29.837430710777603 30.036936171499452 20.41099053462281 64-65 19.81427453077054 29.709373794479077 30.03624916648543 20.44010250826495 66-67 19.793020180662992 29.684981044321002 29.980594463631117 20.541404311384888 68-69 19.774244463053346 29.852926349221498 29.862922116884917 20.509907070840246 70-71 19.877369397919068 29.718327141704755 29.838918108583012 20.565385351793168 72-73 19.803820233808135 29.791233311086028 29.805649964730502 20.599296490375334 74-75 19.755632359158966 29.817509132182202 29.8320596585478 20.594798850111033 76-77 19.791498611299456 29.78540247098438 29.81751966847345 20.605579249242716 78-79 19.95665944027383 29.81669398352787 29.689172822536314 20.537473753661985 80-81 19.812290947415352 29.87738027115292 29.661856776848495 20.648472004583226 82-83 19.884965726776194 29.833550569704997 29.70706236576638 20.574421337752426 84-85 19.822193484349174 29.830761234967447 29.738293771107603 20.608751509575775 86-87 19.71132394991176 29.731920618152795 29.836155456005553 20.720599975929897 88-89 19.825982899938737 29.721434880987996 29.779450047416038 20.673132171657223 90-91 19.879270558182867 29.776142414779986 29.693698742062537 20.650888284974606 92-93 19.885565346298982 29.623465489154604 29.702308712165713 20.788660452380693 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 317.0 1 237.0 2 260.0 3 681.0 4 1683.5 5 3803.5 6 7684.0 7 13446.0 8 20389.5 9 27498.5 10 33753.0 11 39193.0 12 44992.5 13 51917.0 14 55710.0 15 61343.5 16 77491.5 17 105586.5 18 152368.0 19 220812.0 20 320483.0 21 456505.5 22 634651.0 23 863915.5 24 1135318.5 25 1455123.0 26 1843046.5 27 2280789.5 28 2742530.0 29 2979336.0 30 3188745.0 31 3599600.5 32 3975134.0 33 4299479.5 34 4578404.5 35 4813142.0 36 4944493.5 37 4977097.5 38 4946658.5 39 4848971.5 40 4721723.5 41 4547829.0 42 4342297.5 43 4243337.0 44 4108679.5 45 3803074.5 46 3497696.5 47 3207707.0 48 2914131.5 49 2635619.0 50 2356637.0 51 2112569.5 52 1887318.5 53 1674143.5 54 1493488.0 55 1328485.5 56 1167629.0 57 1084625.0 58 1018693.0 59 894524.5 60 783955.0 61 684160.5 62 597214.5 63 521678.0 64 458868.5 65 415012.5 66 379557.5 67 335765.5 68 282220.0 69 237057.5 70 200114.5 71 180224.0 72 168164.5 73 144821.0 74 124793.0 75 110635.5 76 102818.5 77 99389.5 78 92005.5 79 83685.5 80 75056.0 81 65272.5 82 56872.0 83 45080.5 84 28841.5 85 15784.0 86 12459.0 87 9948.5 88 5677.5 89 2935.0 90 1427.5 91 711.0 92 390.0 93 184.0 94 79.5 95 44.5 96 39.0 97 39.0 98 41.5 99 114.5 100 184.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15616103186740107 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 4.5655833435441174E-5 22-23 0.0014130706467246448 24-25 0.02007455353499512 26-27 0.12120900516381942 28-29 0.16514302602940323 30-31 0.196572320951179 32-33 0.2137628723345096 34-35 0.23113152667203393 36-37 0.24308024593143013 38-39 0.24717616183993046 40-41 0.23839894087145558 42-43 0.22888489803664638 44-45 0.23494898720037355 46-47 0.22924743247640106 48-49 0.23537661510562033 50-51 0.23955299377007622 52-53 0.3052182872750204 54-55 0.42046852811858254 56-57 0.44751486303055593 58-59 0.4740359298390444 60-61 0.4930762205334426 62-63 0.4941018946528982 64-65 0.5032231165049807 66-67 0.4949982859172791 68-69 0.4869307645378503 70-71 0.49397035160804953 72-73 0.47526230881043396 74-75 0.46515383606505445 76-77 0.47682138771656113 78-79 0.4348677451309839 80-81 0.42678937484063967 82-83 0.39871058523988856 84-85 0.3646214989920322 86-87 0.3455007455416785 88-89 0.3579213924258925 90-91 0.3460816143136046 92-93 0.29197990373056165 >>END_MODULE >>Sequence Length Distribution pass #Length Count 93 1.10610181E8 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.90378913167984 #Duplication Level Percentage of deduplicated Percentage of total 1 80.33231531671294 44.90880815925134 2 12.419296613219595 13.885714780584276 3 3.3074732676490504 5.547008643399617 4 1.2598586326948458 2.8172348535159655 5 0.6199175706236252 1.7327870573583193 6 0.38653339428631484 1.2965208819920762 7 0.2325656302900172 0.9100909968506536 8 0.15889907264387776 0.7106448200242256 9 0.13381460770578477 0.6732669250730386 >10 0.9528591373553186 10.391601696546298 >50 0.10370962727392588 4.111593834217034 >100 0.08489894663482815 9.202846856278502 >500 0.0061759778632782805 2.3527882728656593 >1k 0.0016729146309377959 1.362850772552267 >5k 3.2577137224047087E-6 0.017239341089989643 >10k+ 6.032701972500366E-6 0.07900210840064928 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 9.040759096126965E-7 9.040759096126965E-7 1.808151819225393E-6 2 2.7122277288380894E-6 1.808151819225393E-6 5.424455457676179E-6 3 2.7122277288380894E-6 3.616303638450786E-6 9.040759096126965E-6 4 3.616303638450786E-6 9.040759096126965E-6 1.536929046341584E-5 5 1.3561138644190447E-5 9.944835005739662E-6 1.6273366373028535E-5 6 1.9889670011479324E-5 1.1752986824965055E-5 1.7177442282641234E-5 7 2.4410049559542805E-5 1.265706273457775E-5 2.1697821830704715E-5 8 3.616303638450786E-5 1.3561138644190447E-5 2.2601897740317414E-5 9 4.610787139024752E-5 1.536929046341584E-5 4.339564366140943E-5 10-11 7.096995890459667E-5 1.898559410186663E-5 9.402389459972044E-5 12-13 8.317498368436808E-5 2.53141254691555E-5 1.1120133688236167E-4 14-15 9.266778073530139E-5 2.847839115279994E-5 1.3967972803516162E-4 16-17 1.1029726097274898E-4 3.706711229412055E-5 1.8578759942540913E-4 18-19 1.3109100689384098E-4 4.2039529796990385E-5 2.4138826786658995E-4 20-21 1.4781641122167587E-4 4.384768161621578E-5 3.1371434063560564E-4 22-23 1.640897775947044E-4 4.701194729986022E-5 4.7554392845627837E-4 24-25 1.7493868851005677E-4 5.0176212983504655E-5 0.0010591249281112739 26-27 1.8126721987734564E-4 5.424455457676179E-5 0.002907508125314432 28-29 1.92116130792698E-4 5.876493412482527E-5 0.013170125813282956 30-31 2.0884153512053287E-4 6.057308594405067E-5 0.02394897084564033 32-33 2.2149859785511062E-4 6.46414275373078E-5 0.03634520768029482 34-35 2.2918324308681857E-4 6.916180708537128E-5 0.06105586247978384 36-37 2.495249510531042E-4 7.729849027188555E-5 0.1225185591188934 38-39 2.639901656069074E-4 8.227090777475539E-5 0.2322557450656373 40-41 2.7529111447706606E-4 8.679128732281887E-5 0.359904482933628 42-43 2.942767085789327E-4 8.859943914204426E-5 0.4966550050216444 44-45 3.100980369971549E-4 9.040759096126964E-5 0.6625099908298677 46-47 3.272754792797961E-4 9.311981869010775E-5 0.862907456954618 48-49 3.4716514929127544E-4 9.764019823817122E-5 1.0646592287919683 50-51 3.697670470315929E-4 9.899631210259027E-5 1.2645083728775384 52-53 3.887526411334595E-4 1.0170853983142836E-4 1.4605454808902265 54-55 4.271758672919991E-4 1.0532484346987914E-4 1.6683739989540385 56-57 4.4209311980060857E-4 1.1255745074678072E-4 1.902636792539016 58-59 4.683113211793768E-4 1.170778302948442E-4 2.1412942991206205 60-61 5.388292421291672E-4 1.1933802006887594E-4 2.3728367282935734 62-63 5.532944566829703E-4 1.2431043757174574E-4 2.5929602266901632 64-65 5.627872537339036E-4 1.2792674121019657E-4 2.814078660625282 66-67 5.745402405588686E-4 1.3425527257748544E-4 3.053034964295014 68-69 5.853891514742209E-4 1.3832361417074257E-4 3.299919561654094 70-71 5.980462142087988E-4 1.4465214553803144E-4 3.5403924526622013 72-73 6.143195805818273E-4 1.478164112216759E-4 3.7722499522896538 74-75 6.28332757180824E-4 1.4917252508609493E-4 3.9971365746160386 76-77 6.396337060509828E-4 1.5595309440819016E-4 4.232675019309479 78-79 6.540989206047859E-4 1.6273366373028536E-4 4.4776587970685995 80-81 6.635917176557192E-4 1.7087034691679964E-4 4.722758296544149 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACTG 46855 0.0 17.124344 5 CTATACT 56720 0.0 14.543617 4 TATACAG 46630 0.0 14.342244 5 TACACTG 33330 0.0 13.793316 5 TACACCG 18965 0.0 13.241094 5 TCTATAC 58545 0.0 13.223505 3 GTATTAG 49475 0.0 13.003043 1 GTATATA 91780 0.0 12.968157 1 CTTACAC 33245 0.0 12.941465 3 GTATAAG 50030 0.0 12.867479 1 GTATAGA 57900 0.0 12.746489 1 TATGCCG 46950 0.0 12.606403 86-87 ACTGCGC 11145 0.0 12.53066 8 GTGTAGG 36445 0.0 12.4433775 1 TATACCG 19790 0.0 12.20696 5 TTATACT 79675 0.0 12.177049 4 GTATAAT 73830 0.0 12.1731615 1 ATGCCGT 41470 0.0 12.078143 86-87 TACACAG 28855 0.0 11.994439 5 ACTGTGC 30360 0.0 11.899847 8 >>END_MODULE