FastQCFastQC Report
Wed 5 Nov 2014
lane5_Undetermined_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane5_Undetermined_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110610181
Sequences flagged as poor quality0
Sequence length93
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG468550.017.1243445
CTATACT567200.014.5436174
TATACAG466300.014.3422445
TACACTG333300.013.7933165
TACACCG189650.013.2410945
TCTATAC585450.013.2235053
GTATTAG494750.013.0030431
GTATATA917800.012.9681571
CTTACAC332450.012.9414653
GTATAAG500300.012.8674791
GTATAGA579000.012.7464891
TATGCCG469500.012.60640386-87
ACTGCGC111450.012.530668
GTGTAGG364450.012.44337751
TATACCG197900.012.206965
TTATACT796750.012.1770494
GTATAAT738300.012.17316151
ATGCCGT414700.012.07814386-87
TACACAG288550.011.9944395
ACTGTGC303600.011.8998478
Produced by FastQC (version 0.11.2)