##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename lane3_Undetermined_L003_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5876217 Sequences flagged as poor quality 0 Sequence length 93 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.67242547373591 33.0 27.0 33.0 15.0 33.0 2 28.63296130827027 33.0 27.0 33.0 15.0 33.0 3 28.648236441914925 33.0 27.0 33.0 15.0 33.0 4 32.00896273912281 37.0 33.0 37.0 15.0 37.0 5 32.00266463951212 37.0 33.0 37.0 15.0 37.0 6 32.01241053555374 37.0 33.0 37.0 15.0 37.0 7 31.891123319645956 37.0 33.0 37.0 15.0 37.0 8 31.891661420944803 37.0 33.0 37.0 15.0 37.0 9 31.945567871302234 37.0 33.0 37.0 15.0 37.0 10-11 31.904605292827 37.0 33.0 37.0 15.0 37.0 12-13 31.858202054144698 37.0 33.0 37.0 15.0 37.0 14-15 33.100642300990586 37.0 33.0 40.0 15.0 40.0 16-17 33.0204761839122 37.0 33.0 40.0 15.0 40.0 18-19 33.02705950103613 37.0 33.0 40.0 15.0 40.0 20-21 32.87549779050025 37.0 33.0 40.0 15.0 40.0 22-23 32.699502588144725 37.0 33.0 40.0 15.0 40.0 24-25 32.53769933955809 37.0 33.0 40.0 15.0 40.0 26-27 32.33993656122638 37.0 30.0 40.0 15.0 40.0 28-29 32.15610400364724 37.0 27.0 40.0 15.0 40.0 30-31 32.00227586898169 37.0 27.0 40.0 15.0 40.0 32-33 31.79150208373857 37.0 27.0 40.0 15.0 40.0 34-35 31.73305904121648 37.0 27.0 40.0 15.0 40.0 36-37 31.670636822976412 37.0 27.0 40.0 15.0 40.0 38-39 31.556142242534612 37.0 27.0 40.0 15.0 40.0 40-41 31.474303876116217 37.0 27.0 40.0 15.0 40.0 42-43 31.53643236796735 37.0 27.0 40.0 15.0 40.0 44-45 31.449550620748006 37.0 27.0 40.0 15.0 40.0 46-47 31.270110429890522 37.0 27.0 40.0 15.0 40.0 48-49 31.020206112197695 37.0 27.0 40.0 10.5 40.0 50-51 29.979479144490412 35.0 24.5 38.5 6.0 38.5 52-53 29.883944466312258 35.0 24.5 37.0 6.0 40.0 54-55 30.392588377862833 37.0 27.0 40.0 6.0 40.0 56-57 30.274001198390053 37.0 27.0 40.0 6.0 40.0 58-59 30.15585077950661 37.0 27.0 40.0 6.0 40.0 60-61 30.36997774248296 37.0 27.0 40.0 6.0 40.0 62-63 30.405979391162717 37.0 27.0 38.5 6.0 40.0 64-65 30.2951480518844 37.0 27.0 37.0 6.0 40.0 66-67 30.151972774320623 33.0 27.0 37.0 6.0 40.0 68-69 30.03108028515625 33.0 27.0 37.0 6.0 40.0 70-71 29.898222104459386 33.0 27.0 37.0 6.0 40.0 72-73 29.581992377068445 33.0 27.0 37.0 6.0 40.0 74-75 29.440054630385504 33.0 27.0 37.0 6.0 40.0 76-77 29.039342148188197 33.0 22.0 37.0 6.0 37.0 78-79 28.643187275078507 33.0 22.0 37.0 6.0 37.0 80-81 28.249882194616028 33.0 22.0 37.0 6.0 37.0 82-83 28.038260670087574 33.0 22.0 37.0 6.0 37.0 84-85 27.924433610943915 33.0 22.0 37.0 6.0 37.0 86-87 27.83011127737454 33.0 22.0 37.0 6.0 37.0 88-89 27.75030993579713 33.0 22.0 37.0 6.0 37.0 90-91 27.689711339795657 33.0 22.0 37.0 6.0 37.0 92-93 26.8560310996003 33.0 18.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2304 1 0.7216429310357384 2304 2 0.6736978468201293 2304 3 0.7791872279511018 2304 4 0.7884789017867107 2304 5 0.778374195006549 2304 6 1.124096827312151 2304 7 1.0155264613508557 2304 8 1.1210933689268678 2304 9 0.9425770549201644 2304 10-11 1.0529286703397176 2304 12-13 1.0643537321100105 2304 14-15 1.3050232858105986 2304 16-17 1.4347952213660662 2304 18-19 1.3602502442131055 2304 20-21 1.3356447768625657 2304 22-23 1.3121771143601748 2304 24-25 1.2365251132800523 2304 26-27 1.1663162890143965 2304 28-29 1.2480264854898238 2304 30-31 1.1834956901136167 2304 32-33 1.3451948537904919 2304 34-35 1.3530063003200183 2304 36-37 1.1448039017031348 2304 38-39 1.248476952186138 2304 40-41 1.207303747838676 2304 42-43 1.0797672070011686 2304 44-45 1.1330529980134578 2304 46-47 1.1610195349656465 2304 48-49 1.2074232855674758 2304 50-51 0.9366288610171232 2304 52-53 1.2206677868035207 2304 54-55 1.4138233975033074 2304 56-57 1.4443244917992821 2304 58-59 1.4070334348525009 2304 60-61 1.3616811143969443 2304 62-63 1.2155729017062136 2304 64-65 1.2416228533211608 2304 66-67 1.25074776176589 2304 68-69 1.2874765209542751 2304 70-71 1.1519118415644307 2304 72-73 1.2865461836098042 2304 74-75 1.2417927600061525 2304 76-77 1.0929322687034997 2304 78-79 1.0032437614545984 2304 80-81 0.9559429741090995 2304 82-83 1.0245849189858056 2304 84-85 0.8532701038339425 2304 86-87 0.8018552805556389 2304 88-89 0.8124142928849487 2304 90-91 0.8129668394165854 2304 92-93 0.8087925654347217 2305 1 0.28713982622485545 2305 2 0.20645485385073314 2305 3 0.2882717786038036 2305 4 0.4887770871772119 2305 5 0.3848528095046788 2305 6 0.5782661648398815 2305 7 0.6312324196331609 2305 8 0.4878778387947982 2305 9 0.615504247089099 2305 10-11 0.6602870272975494 2305 12-13 0.7249152111878026 2305 14-15 0.8372497889585233 2305 16-17 0.8830845207033136 2305 18-19 0.8121719825267775 2305 20-21 0.8739790943046017 2305 22-23 0.7977220384017514 2305 24-25 0.8529127921485014 2305 26-27 0.7535201630966739 2305 28-29 0.75397622194523 2305 30-31 0.8792680623735478 2305 32-33 0.7955058330470344 2305 34-35 0.6042917781634429 2305 36-37 0.777375345594848 2305 38-39 0.8373556225878005 2305 40-41 0.9397332183121101 2305 42-43 0.7227359654219185 2305 44-45 0.7620412310080091 2305 46-47 0.8005964923116693 2305 48-49 0.9161966939267536 2305 50-51 0.812750973645354 2305 52-53 0.6367697571313542 2305 54-55 0.8045122663507343 2305 56-57 0.8733502595059726 2305 58-59 0.7459950049773347 2305 60-61 0.7172700004266517 2305 62-63 0.6820306683165676 2305 64-65 0.9254299265311516 2305 66-67 0.7629402708191932 2305 68-69 0.7599707732250387 2305 70-71 0.7779895039996596 2305 72-73 0.8357473844307499 2305 74-75 0.7025503724470248 2305 76-77 0.6763838943487279 2305 78-79 0.5556182033589891 2305 80-81 0.5041342067648067 2305 82-83 0.4112174756205107 2305 84-85 0.5577117129463289 2305 86-87 0.6565692472999167 2305 88-89 0.6212271969276912 2305 90-91 0.6955866735591698 2305 92-93 0.6165350870916768 2306 1 0.9083137961533971 2306 2 0.8687229980669606 2306 3 1.040445574213873 2306 4 1.172738709089387 2306 5 0.9309529014943152 2306 6 1.1195327217532025 2306 7 1.1336673472661083 2306 8 1.0195326815804613 2306 9 1.078359063768474 2306 10-11 1.1436728221026655 2306 12-13 1.2065965151674227 2306 14-15 1.5156578095994462 2306 16-17 1.572215178529241 2306 18-19 1.5262128285438337 2306 20-21 1.4533691059390463 2306 22-23 1.5237908821227037 2306 24-25 1.3910671743406908 2306 26-27 1.4134556339192486 2306 28-29 1.538318067385127 2306 30-31 1.4088549128425 2306 32-33 1.4523240129796484 2306 34-35 1.519083184585348 2306 36-37 1.4347173336421903 2306 38-39 1.4952493272204066 2306 40-41 1.582808101232498 2306 42-43 1.5741221844542252 2306 44-45 1.4882115684566806 2306 46-47 1.463609868742509 2306 48-49 1.3384796695016128 2306 50-51 1.2666331594312368 2306 52-53 1.5012092817572267 2306 54-55 1.5089260727794276 2306 56-57 1.517788440100464 2306 58-59 1.330511141235565 2306 60-61 1.3704791841617308 2306 62-63 1.3297781344071993 2306 64-65 1.4469164528931096 2306 66-67 1.3399411698821595 2306 68-69 1.3048299868597475 2306 70-71 1.2888648657381445 2306 72-73 1.2820104886214807 2306 74-75 1.2816189872770387 2306 76-77 1.2590479544426039 2306 78-79 1.1218665748271377 2306 80-81 1.0742818133057952 2306 82-83 1.0643922058762705 2306 84-85 1.0307942120445475 2306 86-87 1.008497987641075 2306 88-89 1.0739958093885953 2306 90-91 1.3376110364786165 2306 92-93 1.1731956198867337 2307 1 0.6611143198502454 2307 2 0.6552660653559492 2307 3 0.7483814847932138 2307 4 0.7460533842805432 2307 5 0.8587499285918767 2307 6 0.8290929288672331 2307 7 0.6549814630013806 2307 8 0.7377831995818163 2307 9 0.7875551919876926 2307 10-11 0.8187706187404515 2307 12-13 0.8986394808027036 2307 14-15 1.003870304787128 2307 16-17 0.797010104783439 2307 18-19 1.0105877158956602 2307 20-21 0.9088837116307928 2307 22-23 0.9681545803568099 2307 24-25 0.9745558720340881 2307 26-27 0.7475668704753673 2307 28-29 0.9285039422775228 2307 30-31 0.7496742129170428 2307 32-33 0.9676776885904026 2307 34-35 0.8331134874845674 2307 36-37 0.7256423247384802 2307 38-39 0.761441465711691 2307 40-41 0.688973146486429 2307 42-43 0.531877137008621 2307 44-45 0.6050534976566659 2307 46-47 0.6271043628602868 2307 48-49 0.5527563204371866 2307 50-51 0.595301236272789 2307 52-53 0.6784208988298595 2307 54-55 0.6955637284862419 2307 56-57 0.7139199035822692 2307 58-59 0.7349696297530208 2307 60-61 0.7519188746730414 2307 62-63 0.637748866789746 2307 64-65 0.7807995093101994 2307 66-67 0.8031021533133291 2307 68-69 0.8684194298695047 2307 70-71 0.8683847430039044 2307 72-73 0.8322944701594395 2307 74-75 0.7977026230481847 2307 76-77 0.6706211213233892 2307 78-79 0.5445841606308015 2307 80-81 0.5458933923123581 2307 82-83 0.660588698895328 2307 84-85 0.5561341031236751 2307 86-87 0.5063931790600584 2307 88-89 0.3244972289358117 2307 90-91 0.6203428105449511 2307 92-93 0.787144744006639 2308 1 0.9225917643427621 2308 2 0.8208082280782065 2308 3 0.9156916216858626 2308 4 1.0911196006916413 2308 5 1.114509447785153 2308 6 1.0232601029393464 2308 7 0.9394943137120144 2308 8 1.0282645404140673 2308 9 1.0936192879781217 2308 10-11 1.2296423145962834 2308 12-13 1.206407591171395 2308 14-15 1.3788978115035633 2308 16-17 1.501948728753277 2308 18-19 1.4674227677311293 2308 20-21 1.4765535388992959 2308 22-23 1.513648452768372 2308 24-25 1.4243855573893 2308 26-27 1.261220082885977 2308 28-29 1.273556371948466 2308 30-31 1.1874125599503245 2308 32-33 1.3396934769115312 2308 34-35 1.4408431752432804 2308 36-37 1.4726353465782722 2308 38-39 1.5355912945291124 2308 40-41 1.4549110186950251 2308 42-43 1.5097715846577273 2308 44-45 1.3143670915827563 2308 46-47 1.423398400312152 2308 48-49 1.3216108392832986 2308 50-51 1.1430566347997093 2308 52-53 1.2896736495015055 2308 54-55 1.4095577037141709 2308 56-57 1.4258827548270112 2308 58-59 1.3026805168045712 2308 60-61 1.2283721907011795 2308 62-63 1.2889753859349575 2308 64-65 1.2870826241921307 2308 66-67 1.3007975311167392 2308 68-69 1.3461757430615116 2308 70-71 1.366314295098423 2308 72-73 1.3807171402337346 2308 74-75 1.2399185894953355 2308 76-77 1.21888672938206 2308 78-79 1.072402330552876 2308 80-81 1.0649108483950833 2308 82-83 1.0271735464333425 2308 84-85 1.1228520778281776 2308 86-87 0.9368977270152179 2308 88-89 1.145230383074903 2308 90-91 1.0549504659578979 2308 92-93 1.1946211630060333 2309 1 0.6208982955176552 2309 2 0.5024592609345468 2309 3 0.5268365256530849 2309 4 0.6444598283682694 2309 5 0.781225395961016 2309 6 0.762634696092384 2309 7 0.7855943547064221 2309 8 0.5790682109902185 2309 9 0.7138552930055653 2309 10-11 0.6487696405220973 2309 12-13 0.7655324506754546 2309 14-15 0.848642531221202 2309 16-17 0.8389286262897784 2309 18-19 0.8594211859551706 2309 20-21 0.8361588016126262 2309 22-23 0.654681869404449 2309 24-25 0.7553036462840907 2309 26-27 0.6247852898964652 2309 28-29 0.7457439613421712 2309 30-31 0.460731085367982 2309 32-33 0.6588758186832528 2309 34-35 0.6391138503896343 2309 36-37 0.7612041856482037 2309 38-39 0.6677532089228819 2309 40-41 0.46686302820933534 2309 42-43 0.5705093178697354 2309 44-45 0.5404542739605667 2309 46-47 0.5985116558086645 2309 48-49 0.5946602262817642 2309 50-51 0.4664909074755883 2309 52-53 0.44475882833397407 2309 54-55 0.7102571989423794 2309 56-57 0.3753768145797416 2309 58-59 0.22842037294122974 2309 60-61 0.3323316985880993 2309 62-63 0.2391115740857721 2309 64-65 0.5112030604672242 2309 66-67 0.7102002009338193 2309 68-69 0.519051790727648 2309 70-71 0.6550929630279576 2309 72-73 0.5671149228192185 2309 74-75 0.5354963318972885 2309 76-77 0.6072482031985693 2309 78-79 0.35446342010313003 2309 80-81 0.17524046764263446 2309 82-83 0.171524018770004 2309 84-85 0.20942819734714746 2309 86-87 0.2559633360862428 2309 88-89 0.31394003624419753 2309 90-91 0.4408394082796683 2309 92-93 0.5001043057454062 2310 1 -0.4693936816666273 2310 2 -0.9193434151142803 2310 3 -0.690491476905688 2310 4 -0.7717250002473257 2310 5 -0.7475924301491155 2310 6 -0.6934070332108853 2310 7 -0.5196610853490178 2310 8 -0.6851983703840503 2310 9 -0.691366158031272 2310 10-11 -0.885057215920142 2310 12-13 -0.8507097797206242 2310 14-15 -1.0147557096231878 2310 16-17 -0.9370580787584615 2310 18-19 -0.8030220679496196 2310 20-21 -0.9363715784715936 2310 22-23 -0.8549607021612005 2310 24-25 -0.7486524707797173 2310 26-27 -0.9363986964567523 2310 28-29 -0.7793109989435578 2310 30-31 -0.89683955272573 2310 32-33 -0.8234032367034949 2310 34-35 -1.0159838200618836 2310 36-37 -0.83164117924332 2310 38-39 -0.9011185646258184 2310 40-41 -1.0537058827134231 2310 42-43 -1.1755525937460263 2310 44-45 -1.0832026267270969 2310 46-47 -0.8092309424108528 2310 48-49 -0.9940924471016821 2310 50-51 -1.0618419567495856 2310 52-53 -0.9196017026111711 2310 54-55 -1.0529868972247947 2310 56-57 -0.7682461184124243 2310 58-59 -0.8549887320217202 2310 60-61 -0.7017339125852047 2310 62-63 -0.7633077126565944 2310 64-65 -0.7092997701740629 2310 66-67 -0.885023595643819 2310 68-69 -0.8552390456708814 2310 70-71 -0.7278169255588551 2310 72-73 -0.8079492916911519 2310 74-75 -0.6076900959506766 2310 76-77 -0.6842541156883648 2310 78-79 -0.8170657445644949 2310 80-81 -0.8157339872856717 2310 82-83 -0.8062590826746394 2310 84-85 -0.7608528517492843 2310 86-87 -0.9555849020927631 2310 88-89 -0.8542106967373044 2310 90-91 -1.0059382664364698 2310 92-93 -0.8559605271348119 2311 1 0.6783202508107848 2311 2 0.578354540187668 2311 3 0.6588437584723437 2311 4 0.683458261759327 2311 5 0.787106190868478 2311 6 0.9216740889954878 2311 7 0.7453011590637253 2311 8 0.840738671936446 2311 9 0.9445332238041999 2311 10-11 0.8471520089966447 2311 12-13 0.8486701537078183 2311 14-15 0.8269795257548367 2311 16-17 0.9114764909039224 2311 18-19 0.6809727014274642 2311 20-21 0.6902930910481331 2311 22-23 0.7790121937018526 2311 24-25 0.7801195691632188 2311 26-27 0.6976368717791033 2311 28-29 0.7927536705761753 2311 30-31 0.7656802760902508 2311 32-33 0.7253497827924669 2311 34-35 0.7346687714740199 2311 36-37 0.7040580123876907 2311 38-39 0.5416751586266813 2311 40-41 0.5493837035918538 2311 42-43 0.5023607681179811 2311 44-45 0.23695743033423966 2311 46-47 0.3247004888777809 2311 48-49 0.6181585276962274 2311 50-51 0.5364202401120401 2311 52-53 0.6127931618681437 2311 54-55 0.7441039254095365 2311 56-57 0.7195875477787332 2311 58-59 0.5559403217110415 2311 60-61 0.5897618553653388 2311 62-63 0.4510469235773584 2311 64-65 0.4975923838915577 2311 66-67 0.6263955199053903 2311 68-69 0.5046328516187053 2311 70-71 0.3765540682594981 2311 72-73 0.40176692323989727 2311 74-75 0.409043807996067 2311 76-77 0.18106400128738542 2311 78-79 0.3831221774516749 2311 80-81 0.31515835698442274 2311 82-83 0.37033359445327463 2311 84-85 0.28610264040477773 2311 86-87 0.2640716837370043 2311 88-89 0.110412793514719 2311 90-91 0.08957328848507373 2311 92-93 -0.12390945723466729 2312 1 0.09370429813134962 2312 2 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0.6006400483134726 1112 7 0.6263879257460125 1112 8 0.8140730237307601 1112 9 0.47743866589540573 1112 10-11 0.7695380566317169 1112 12-13 0.7432535584366384 1112 14-15 0.8929033352393247 1112 16-17 0.474609166779409 1112 18-19 0.6914694901786973 1112 20-21 0.305653409468583 1112 22-23 0.6223403108634002 1112 24-25 0.6841654742275196 1112 26-27 0.6170546561722929 1112 28-29 0.5667246981138518 1112 30-31 0.7771291882294094 1112 32-33 1.0817350170350473 1112 34-35 0.8675416985102302 1112 36-37 0.6229759939356541 1112 38-39 0.6263986567924178 1112 40-41 0.7163488206150852 1112 42-43 0.8369335615844165 1112 44-45 0.8129771127808603 1112 46-47 0.47961604263497293 1112 48-49 0.5971163324448696 1112 50-51 0.7017091286339188 1112 52-53 0.6519189920342683 1112 54-55 0.4950410815499282 1112 56-57 0.5816198729045077 1112 58-59 0.6290661642638575 1112 60-61 0.6270213213752385 1112 62-63 0.4812459776473119 1112 64-65 -0.04574347494975228 1112 66-67 0.4257677212881603 1112 68-69 0.5300984362393919 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28-29 -0.4470614829008852 2302 30-31 -0.29560821700350104 2302 32-33 0.03540712616153385 2302 34-35 0.05349981600220133 2302 36-37 -0.1374930594690298 2302 38-39 0.006580829912671504 2302 40-41 0.13717381579145638 2302 42-43 -0.4818960428172403 2302 44-45 -0.08000546868928993 2302 46-47 0.15360918280023128 2302 48-49 0.3268393622809107 2302 50-51 0.3646719935778613 2302 52-53 0.4311506018494704 2302 54-55 -0.24458968152398342 2302 56-57 -0.21279694011018435 2302 58-59 0.24153350530271567 2302 60-61 0.21710297526025713 2302 62-63 0.0787177167268247 2302 64-65 -0.11713614729008981 2302 66-67 -0.3199221005245825 2302 68-69 0.07229198114241342 2302 70-71 -0.3608720513877621 2302 72-73 -0.46622114802790193 2302 74-75 -0.3312573680788553 2302 76-77 -0.045160578063256906 2302 78-79 0.27178814562867615 2302 80-81 0.2301053718312609 2302 82-83 0.2339167565279645 2302 84-85 -0.3279059346604427 2302 86-87 -0.22322008476091426 2302 88-89 -0.1515817746235406 2302 90-91 -0.038555985452902064 2302 92-93 -0.1747761208577785 2303 1 -0.7005418266315324 2303 2 -0.6113133907624686 2303 3 -0.7370028649589742 2303 4 -0.772502711176994 2303 5 -1.1650680926616523 2303 6 -1.6536403522196252 2303 7 -1.2538021539638287 2303 8 -0.9781691195603877 2303 9 -1.4189964688749903 2303 10-11 -1.4222013213491635 2303 12-13 -1.4992068431785803 2303 14-15 -2.110454979068834 2303 16-17 -1.8512578495649379 2303 18-19 -1.9055672620478887 2303 20-21 -1.7999059431757942 2303 22-23 -1.3319568345204615 2303 24-25 -1.3917880960483018 2303 26-27 -1.0101676980324577 2303 28-29 -0.8830706322339168 2303 30-31 -0.7939290241481629 2303 32-33 -1.0500168737182491 2303 34-35 -0.8557718447001861 2303 36-37 -1.136956205743818 2303 38-39 -1.121051181729385 2303 40-41 -0.8165350189403249 2303 42-43 -0.7377902960809344 2303 44-45 -1.1470297002122756 2303 46-47 -1.050757142095712 2303 48-49 -0.6576517366017534 2303 50-51 -0.788258544630974 2303 52-53 -0.42879482680541514 2303 54-55 -0.5704644792331024 2303 56-57 -0.830821193616547 2303 58-59 -0.3594425925001623 2303 60-61 -0.4886253035034116 2303 62-63 -0.8248654264511792 2303 64-65 -0.6003793576257195 2303 66-67 -0.8514719841268494 2303 68-69 -0.6236423976261882 2303 70-71 -0.5768902101885658 2303 72-73 -0.7906413988391208 2303 74-75 -0.5931287437088777 2303 76-77 -0.5430203382925747 2303 78-79 -0.8651211234500416 2303 80-81 -0.6023314375077007 2303 82-83 -0.6260780484388526 2303 84-85 -0.6830287864291336 2303 86-87 -0.6762244093266858 2303 88-89 -0.600587806033964 2303 90-91 -0.6311156622250351 2303 92-93 -1.140897957302407 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 70707.0 3 7402.0 4 6439.0 5 3265.0 6 3069.0 7 10621.0 8 21142.0 9 19416.0 10 21550.0 11 36642.0 12 62323.0 13 81763.0 14 89047.0 15 91486.0 16 92507.0 17 91368.0 18 91476.0 19 92620.0 20 93946.0 21 95442.0 22 98605.0 23 104087.0 24 111036.0 25 115611.0 26 125192.0 27 133391.0 28 144026.0 29 156019.0 30 171873.0 31 192415.0 32 217204.0 33 247494.0 34 287584.0 35 355968.0 36 496542.0 37 831827.0 38 965429.0 39 39683.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.91430960624885 23.299581682544186 10.470318397817438 24.315790313389524 2 16.795288995678554 21.862186352045278 37.68415355118563 23.658371101090534 3 16.476925242046033 26.025944378097794 30.886893084643884 26.61023729521229 4 12.50611552336078 16.53170418789656 40.92170884503627 30.04047144370639 5 13.110869880542461 36.516211057269324 37.129170671088346 13.24374839109987 6 29.844784743534596 39.68536668020943 18.168101559998266 12.301747016257709 7 27.27485148063104 31.73540998328066 25.08026976309143 15.909468772996869 8 24.226811167649842 35.56150560508389 22.891007942964396 17.320675284301874 9 23.62230532446456 14.952609409832828 25.63044039633785 35.79464486936476 10-11 23.365792471664356 26.20941484375648 30.886809167503117 19.537983517076043 12-13 22.564749175121545 25.2380857525212 30.94101982165914 21.256145250698115 14-15 20.565750177315977 26.352044558968878 26.99678294155254 26.085422322162604 16-17 20.92914561618578 30.588714855602735 27.93726053297566 20.544878995235823 18-19 21.11342403216304 29.506971972245523 29.3148527259355 20.064751269655943 20-21 21.217238604567882 29.001231821996328 29.242548124114155 20.538981449321636 22-23 21.454170220029777 28.772417750633377 29.130723004399282 20.64268902493756 24-25 20.96397733785167 28.723029786629432 29.429430730264727 20.883562145254164 26-27 21.330769326927484 29.231747774550776 29.089345414442608 20.348137484079132 28-29 21.331957131635544 29.076956278384703 29.038570846090483 20.55251574388927 30-31 21.101491529750515 28.86869387373949 29.29143685487569 20.738377741634306 32-33 21.28507268976246 29.19820575688209 29.139411320158963 20.37731023319649 34-35 21.169493574646694 28.915217392435633 29.2144334929141 20.700855540003577 36-37 21.114833624600244 29.069918258244655 29.330976183994796 20.484271933160304 38-39 21.241930940664425 29.018689020973603 29.301241853057125 20.438138185304847 40-41 21.05706468343255 28.939942891381538 29.45795244776921 20.545039977416703 42-43 21.004689066401895 28.96541924806745 29.53477439750557 20.49511728802508 44-45 20.99682860698038 28.857258995024477 29.458357637676553 20.687554760318598 46-47 21.03174373630658 28.88454926125365 29.590544074363557 20.49316292807621 48-49 21.027933876167793 29.02455016646646 29.455679602974367 20.491836354391378 50-51 20.779635908540982 28.98949063032605 29.640943759731865 20.58992970140111 52-53 20.905736832259112 28.8220497093496 29.76284904642319 20.5093644119681 54-55 20.881407963483888 29.027901203129176 29.591595904013946 20.499094929372987 56-57 20.918650589089506 28.93035560342464 29.50289225288622 20.648101554599634 58-59 20.946563885869644 28.951194217649817 29.515354628155116 20.586887268325427 60-61 20.75673139492698 28.94820101637651 29.658743350350587 20.636324238345924 62-63 20.652240682547465 29.002045821401268 29.609107693146537 20.736605802904727 64-65 20.88029147349307 28.859469944304067 29.627034838200895 20.633203744001968 66-67 20.767158231370146 28.799910558634988 29.642489663784232 20.79044154621063 68-69 20.746754228601162 28.988550858789868 29.520434690571122 20.74426022203784 70-71 20.916687393396867 28.883108442165657 29.516058104709618 20.684146059727855 72-73 20.860845689544966 28.965014421224726 29.415660833053625 20.758479056176682 74-75 20.783216513353846 29.031480032249057 29.528705173860182 20.656598280536915 76-77 20.86788032707804 29.024596757381925 29.423737832708973 20.68378508283106 78-79 21.02858142901171 28.882131256481525 29.404703209072007 20.684584105434762 80-81 20.9816079946201 29.036834030507457 29.32659569390843 20.65496228096401 82-83 21.024586876674345 29.054050663125373 29.328214504789525 20.59314795541076 84-85 21.002621559674477 29.04534942051442 29.38122523919254 20.570803780618565 86-87 20.884037981212167 28.988883383512476 29.452037134458276 20.67504150081708 88-89 21.01432993036512 28.886482295054115 29.44265523002163 20.656532544559138 90-91 21.21163949470999 28.9541891561734 29.331146331018825 20.503025018097787 92-93 21.331674903860836 28.888143646485542 29.24207979897544 20.538101650678183 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 67717.0 1 35925.5 2 4235.0 3 4227.5 4 3923.5 5 3344.5 6 2779.0 7 2436.5 8 2311.5 9 2432.0 10 2636.5 11 2883.5 12 3241.5 13 3794.5 14 4117.0 15 4463.0 16 5414.0 17 6837.0 18 8850.0 19 11936.5 20 16426.0 21 22464.0 22 30483.5 23 40495.5 24 52718.5 25 67480.0 26 84792.5 27 104047.5 28 124510.0 29 135059.0 30 145386.5 31 165462.0 32 184240.0 33 200724.5 34 215382.5 35 228016.0 36 236320.0 37 241005.5 38 242713.0 39 240720.5 40 235952.5 41 229828.0 42 222708.5 43 218834.0 44 213686.5 45 201673.0 46 188396.5 47 175592.0 48 163187.5 49 151384.5 50 139797.5 51 127865.0 52 116352.0 53 104906.5 54 93980.0 55 84128.0 56 75139.0 57 71146.0 58 67673.0 59 61133.0 60 55025.5 61 49214.0 62 43879.5 63 38615.0 64 33435.5 65 29108.0 66 25581.0 67 22358.0 68 19614.5 69 17546.5 70 15203.0 71 13729.0 72 12667.5 73 10767.5 74 9359.0 75 8259.5 76 7446.0 77 6963.5 78 5936.5 79 5363.5 80 4644.0 81 3213.0 82 2421.0 83 1871.5 84 1516.5 85 1135.0 86 895.0 87 773.5 88 585.5 89 448.5 90 305.0 91 198.0 92 123.5 93 74.5 94 53.0 95 36.5 96 34.0 97 30.0 98 24.5 99 48.5 100 73.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.8682598004804793 2 1.779018712208892 3 1.6902030677219717 4 1.6310663816533664 5 1.565394879052288 6 1.524926666254837 7 1.5333504531912283 8 1.6188306184063659 9 1.609606997154802 10-11 1.5293002283612058 12-13 1.3263380164483374 14-15 1.2305961471470506 16-17 1.1817381829159816 18-19 1.141286987869917 20-21 1.3946813060171195 22-23 1.6019830445335832 24-25 1.7854939666115122 26-27 1.9822446992682536 28-29 2.0221257996428657 30-31 2.1387569587712636 32-33 2.124198272459986 34-35 1.8944160843617588 36-37 1.7263657894186004 38-39 1.6738575175150952 40-41 1.6158865474164759 42-43 1.5513977785367694 44-45 1.4477171282136108 46-47 1.4445603353313876 48-49 1.7371890793005094 50-51 1.987418095689795 52-53 2.5747517492972096 54-55 2.766652422808756 56-57 3.025832776427419 58-59 3.053546184560577 60-61 2.8321537478959677 62-63 2.62184837625976 64-65 2.461609229203074 66-67 2.278804543807691 68-69 2.083364178007722 70-71 1.9183345339356936 72-73 2.2240924730996148 74-75 2.1015391364886624 76-77 2.6515103169266894 78-79 3.1489476988341307 80-81 3.5474438741795957 82-83 3.357832088229553 84-85 3.0110868948508878 86-87 2.6470516660633874 88-89 2.33746473283747 90-91 2.1006967577950233 92-93 1.935547989463289 >>END_MODULE >>Sequence Length Distribution pass #Length Count 93 5876217.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.73702334496966 #Duplication Level Percentage of deduplicated Percentage of total 1 94.66194498395399 83.05357276937404 2 3.4559909912297417 6.0643672455505735 3 0.7606957814101656 2.002235505960109 4 0.41232455006422397 1.4470451469875572 5 0.203128949911357 0.891096471020595 6 0.11763160586737173 0.6192388170055118 7 0.09282414056602661 0.5700879651472754 8 0.060073907460056086 0.42165646569971893 9 0.033004220125039535 0.26061228284337995 >10 0.19481742545175337 2.800881756869787 >50 0.005210582433558344 0.3103739797435504 >100 0.0021458437654254324 0.309303624888816 >500 8.958778392998396E-5 0.059643730453482216 >1k 9.824433218810895E-5 0.09599732357309324 >5k 0.0 0.0 >10k+ 1.9185645166188967E-5 1.0938869148825714 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 64985 1.1058985738613807 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 1.701775138664893E-5 5 0.0 0.0 1.701775138664893E-5 6 0.0 0.0 1.701775138664893E-5 7 0.0 1.701775138664893E-5 1.701775138664893E-5 8 0.0 1.701775138664893E-5 1.701775138664893E-5 9 3.403550277329786E-5 1.701775138664893E-5 3.403550277329786E-5 10-11 5.1053254159946785E-5 1.701775138664893E-5 1.191242597065425E-4 12-13 6.807100554659571E-5 1.701775138664893E-5 1.4465088678651587E-4 14-15 1.0210650831989356E-4 1.701775138664893E-5 1.5315976247984035E-4 16-17 1.1912425970654249E-4 1.701775138664893E-5 1.9570414094646267E-4 18-19 1.3614201109319143E-4 2.5526627079973393E-5 2.978106492663562E-4 20-21 1.5315976247984035E-4 3.403550277329786E-5 3.7439053050627644E-4 22-23 1.6166863817316482E-4 4.254437846662232E-5 5.530769200660902E-4 24-25 1.7017751386648927E-4 5.1053254159946785E-5 0.0012422958512253717 26-27 1.871952652531382E-4 6.807100554659571E-5 0.005173396421541274 28-29 2.3824851941308498E-4 1.0210650831989356E-4 0.025722331220919853 30-31 2.552662707997339E-4 1.1912425970654249E-4 0.043556934674127926 32-33 2.807928978797073E-4 1.1912425970654249E-4 0.06254023634593481 34-35 2.8930177357303175E-4 1.4465088678651587E-4 0.09461018883407471 36-37 2.8930177357303175E-4 1.5315976247984035E-4 0.16405112336729566 38-39 2.8930177357303175E-4 1.5315976247984035E-4 0.2792442825035223 40-41 2.8930177357303175E-4 1.5315976247984035E-4 0.4090046368267203 42-43 2.978106492663562E-4 1.7017751386648927E-4 0.5414708136203956 44-45 3.318461520396541E-4 1.7017751386648927E-4 0.6945795228460759 46-47 3.4035502773297854E-4 1.7868638955981372E-4 0.8733169656600497 48-49 3.4035502773297854E-4 1.871952652531382E-4 1.0551601481020867 50-51 3.5737277911962744E-4 2.0421301663978714E-4 1.2375138630857232 52-53 3.9140828189292533E-4 2.0421301663978714E-4 1.4072659331675466 54-55 4.509704117461966E-4 2.0421301663978714E-4 1.5860714469870665 56-57 4.7649703882616997E-4 2.0421301663978714E-4 1.7862852920509913 58-59 5.105325415994678E-4 2.0421301663978714E-4 1.987103267289142 60-61 5.786035471460635E-4 2.0421301663978714E-4 2.184841029526309 62-63 5.786035471460635E-4 2.0421301663978714E-4 2.3738061409236586 64-65 6.126390499193614E-4 2.0421301663978714E-4 2.5572830274988143 66-67 6.211479256126858E-4 2.2123076802643606E-4 2.754654908081169 68-69 6.466745526926592E-4 2.4675739510640946E-4 2.9582127412925696 70-71 6.466745526926592E-4 2.637751464930584E-4 3.1578820183121215 72-73 6.636923040793082E-4 2.7228402218638286E-4 3.3466173900657514 74-75 6.636923040793082E-4 2.7228402218638286E-4 3.5269034482559105 76-77 6.636923040793082E-4 2.7228402218638286E-4 3.7139200271194888 78-79 6.636923040793082E-4 2.8930177357303175E-4 3.9104665467595905 80-81 6.636923040793082E-4 2.978106492663562E-4 4.105124095995774 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACTG 2420 0.0 14.82345 5 GTATTAG 2300 0.0 13.925639 1 GTCTGGG 2405 0.0 13.857565 1 GTATAGA 2755 0.0 13.668124 1 TACACCG 1090 0.0 13.481523 5 TTATACT 3920 0.0 13.010832 4 TACACTG 1875 0.0 12.908426 5 ATACCGT 1130 0.0 12.609374 6 GTGTAGG 2175 0.0 12.33797 1 TATACCG 965 0.0 12.092693 5 GTATAAG 2295 0.0 12.070035 1 CTACACT 2095 0.0 11.966112 4 GTCTTAG 2055 0.0 11.794715 1 GTCCTAT 2130 0.0 11.785816 1 TAGTACT 2060 0.0 11.749785 4 CTAGTAC 1590 0.0 11.690905 3 ATACAGT 2435 0.0 11.525836 6 TTACACC 1890 0.0 11.434504 4 ATTATAC 3220 0.0 11.411425 3 CTGTCCG 795 1.8189894E-12 11.40332 9 >>END_MODULE