##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename lane3_Undetermined_L003_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5876217 Sequences flagged as poor quality 0 Sequence length 93 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.782012645210344 33.0 33.0 33.0 27.0 33.0 2 31.91598744566445 33.0 33.0 33.0 27.0 33.0 3 31.990312474845638 33.0 33.0 33.0 27.0 33.0 4 35.90445162253198 37.0 37.0 37.0 33.0 37.0 5 35.48418089393227 37.0 37.0 37.0 33.0 37.0 6 35.40708112038749 37.0 37.0 37.0 33.0 37.0 7 35.27403225578633 37.0 37.0 37.0 33.0 37.0 8 35.239696559878574 37.0 37.0 37.0 33.0 37.0 9 35.34431897256347 37.0 37.0 37.0 33.0 37.0 10-11 35.25401444500773 37.0 37.0 37.0 33.0 37.0 12-13 35.224719747415726 37.0 37.0 37.0 33.0 37.0 14-15 36.589695887677394 37.0 37.0 40.0 33.0 40.0 16-17 36.51999866580829 37.0 37.0 40.0 33.0 40.0 18-19 36.46149036020964 37.0 37.0 40.0 33.0 40.0 20-21 36.3606657991017 37.0 37.0 40.0 33.0 40.0 22-23 36.26091420721869 37.0 37.0 40.0 27.0 40.0 24-25 36.09176226813952 37.0 35.0 40.0 27.0 40.0 26-27 35.183867784324505 37.0 33.0 40.0 27.0 40.0 28-29 34.66646781424171 37.0 33.0 40.0 24.5 40.0 30-31 34.30871400766854 37.0 33.0 40.0 22.0 40.0 32-33 34.02968993486796 37.0 33.0 40.0 22.0 40.0 34-35 33.87950011036011 37.0 33.0 40.0 22.0 40.0 36-37 33.683735386218714 37.0 33.0 40.0 18.5 40.0 38-39 33.5179413047544 37.0 33.0 40.0 15.0 40.0 40-41 33.32649823857764 37.0 33.0 40.0 15.0 40.0 42-43 33.23466109233202 37.0 33.0 40.0 15.0 40.0 44-45 33.09630830515619 37.0 33.0 40.0 15.0 40.0 46-47 32.949870129030295 37.0 33.0 40.0 15.0 40.0 48-49 32.75185582833309 37.0 33.0 40.0 15.0 40.0 50-51 32.51709603304303 37.0 33.0 40.0 15.0 40.0 52-53 32.26457863962478 37.0 33.0 40.0 15.0 40.0 54-55 31.902459269288386 37.0 30.0 40.0 15.0 40.0 56-57 31.705554185626568 37.0 27.0 40.0 15.0 40.0 58-59 31.4712140480857 37.0 27.0 38.5 15.0 40.0 60-61 31.198227788388344 37.0 27.0 37.0 15.0 40.0 62-63 30.934019965566282 37.0 27.0 37.0 15.0 40.0 64-65 30.66625185897662 37.0 27.0 37.0 10.5 40.0 66-67 30.4668107049144 33.0 27.0 37.0 10.5 40.0 68-69 30.18092635108608 33.0 27.0 37.0 6.0 40.0 70-71 29.964179420195002 33.0 27.0 37.0 6.0 40.0 72-73 29.717782801417986 33.0 27.0 37.0 6.0 40.0 74-75 29.433178352671455 33.0 27.0 37.0 6.0 37.0 76-77 28.051406542678734 33.0 24.5 37.0 6.0 37.0 78-79 29.077339979105606 33.0 27.0 37.0 6.0 37.0 80-81 29.159738740077163 33.0 27.0 37.0 6.0 37.0 82-83 29.036019857673736 33.0 27.0 37.0 6.0 37.0 84-85 28.884827602520467 33.0 27.0 37.0 6.0 37.0 86-87 28.667951932340145 33.0 27.0 37.0 6.0 37.0 88-89 28.397640948249528 33.0 27.0 37.0 6.0 37.0 90-91 28.207036687038617 33.0 27.0 37.0 6.0 37.0 92-93 27.206802012246996 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2304 1 0.46415460087527904 2304 2 0.3188533646627505 2304 3 0.3340010011220187 2304 4 0.2227481259320072 2304 5 0.044644302987187245 2304 6 0.07899401483866342 2304 7 0.23958919199751705 2304 8 0.17946616775522983 2304 9 0.05422621943733219 2304 10-11 0.062498695369569646 2304 12-13 0.04254528609199326 2304 14-15 0.2591889631107449 2304 16-17 0.2748444628904636 2304 18-19 0.2515333474918222 2304 20-21 0.33388422995277267 2304 22-23 0.2676836576475523 2304 24-25 0.2724370112210579 2304 26-27 0.2848142699245244 2304 28-29 -0.06988273671720435 2304 30-31 -0.07524702307347297 2304 32-33 -0.11485754428859707 2304 34-35 -0.2270197049353797 2304 36-37 -0.27796238117862515 2304 38-39 -0.25443960377464947 2304 40-41 -0.13948360631087553 2304 42-43 -0.13921932033829876 2304 44-45 -0.07085031709279122 2304 46-47 -0.13465997516661332 2304 48-49 -0.04702188687821973 2304 50-51 -0.1524618438966172 2304 52-53 -0.12014366018115652 2304 54-55 -0.1989823163447113 2304 56-57 -0.08626214700500512 2304 58-59 -0.07629196846319886 2304 60-61 -0.20501662095267648 2304 62-63 -0.13764498891295318 2304 64-65 -0.008950472672772491 2304 66-67 -0.2689594525423864 2304 68-69 -0.3027856196180032 2304 70-71 -0.23124979330150808 2304 72-73 -0.18058252283972465 2304 74-75 -0.22915664340819575 2304 76-77 -0.05866207131800394 2304 78-79 0.0653804509799123 2304 80-81 0.15212687489987076 2304 82-83 0.13738537792393757 2304 84-85 0.23792053634411658 2304 86-87 0.1224963916582098 2304 88-89 0.27605277245424276 2304 90-91 0.2712649803202396 2304 92-93 0.3494114444497036 2305 1 0.7416064558590492 2305 2 0.5702751141625626 2305 3 0.5412928345052137 2305 4 0.5507681327306457 2305 5 0.44582756123750755 2305 6 0.41478716705432817 2305 7 0.45358505647655534 2305 8 0.4914118706783057 2305 9 0.5500169695763404 2305 10-11 0.4381958931370207 2305 12-13 0.5590148185303079 2305 14-15 0.6841530972621968 2305 16-17 0.7996209280100359 2305 18-19 0.7493042257905032 2305 20-21 0.7771136798111016 2305 22-23 0.7644017026687422 2305 24-25 0.7711969119750677 2305 26-27 0.6252129421884334 2305 28-29 0.333973782860312 2305 30-31 0.3174968803210163 2305 32-33 0.1908964256367227 2305 34-35 0.2616971694810317 2305 36-37 0.18072160598239861 2305 38-39 0.25425771165107847 2305 40-41 0.15405525583845758 2305 42-43 -0.09277022728979745 2305 44-45 0.20324046473877644 2305 46-47 0.16585507644316522 2305 48-49 0.07385580493987476 2305 50-51 0.13422668420373896 2305 52-53 -0.04535579153780134 2305 54-55 -0.10772346604635175 2305 56-57 -9.598000107757798E-5 2305 58-59 -0.2448783717129963 2305 60-61 -0.2788231661043383 2305 62-63 -0.21738725387740843 2305 64-65 -0.24286726970100858 2305 66-67 -0.1170577904085981 2305 68-69 -0.11720798856737957 2305 70-71 -0.1652383582572554 2305 72-73 0.051475744366303644 2305 74-75 -0.029871528412396486 2305 76-77 -0.03842842498104204 2305 78-79 0.08394493935615444 2305 80-81 0.2522378803691261 2305 82-83 0.14060685075017432 2305 84-85 -0.10553661715700713 2305 86-87 0.20324930410822617 2305 88-89 0.34667063330716275 2305 90-91 0.21665428385115604 2305 92-93 0.31008473059473474 2306 1 0.6358875562427997 2306 2 0.5267782302781185 2306 3 0.42660367771344454 2306 4 0.5676487704836788 2306 5 0.4759554350379389 2306 6 0.5556697255369798 2306 7 0.4949644456539275 2306 8 0.516853794741273 2306 9 0.4672335783012471 2306 10-11 0.4419946024059769 2306 12-13 0.48716663895075385 2306 14-15 0.8083020271855261 2306 16-17 0.7635308738514723 2306 18-19 0.8014123131997337 2306 20-21 0.7597315883747484 2306 22-23 0.808640914996289 2306 24-25 0.9204182034232957 2306 26-27 0.7353288432319545 2306 28-29 0.54396723232054 2306 30-31 0.4853475943860772 2306 32-33 0.3609747933594605 2306 34-35 0.29255872954747275 2306 36-37 0.20626488549824273 2306 38-39 0.4422989942478708 2306 40-41 0.368975114971434 2306 42-43 0.36952376971111534 2306 44-45 0.24149970534629972 2306 46-47 0.36975523795738496 2306 48-49 0.1609211299350477 2306 50-51 0.44453907839468343 2306 52-53 0.23523233004560495 2306 54-55 0.4048016296836181 2306 56-57 0.31878065036291403 2306 58-59 0.5375310368385264 2306 60-61 0.47465919339160934 2306 62-63 0.4751789113217484 2306 64-65 0.15995628856952848 2306 66-67 0.31873516644292366 2306 68-69 0.31141737706585104 2306 70-71 0.4828986971764806 2306 72-73 0.3815541572588259 2306 74-75 0.2821950894524683 2306 76-77 0.20203095720147246 2306 78-79 0.5679919874604025 2306 80-81 0.773632177417209 2306 82-83 0.6421909733332072 2306 84-85 0.6938519269205408 2306 86-87 0.9490661677566088 2306 88-89 0.8806441837282968 2306 90-91 0.9506377485778472 2306 92-93 0.9583111346166504 2307 1 0.7112864929882363 2307 2 0.5277753017861002 2307 3 0.4895489534112869 2307 4 0.4450010445709225 2307 5 0.522313132098347 2307 6 0.532075250308047 2307 7 0.356199920347926 2307 8 0.42827880674506247 2307 9 0.36662884686170116 2307 10-11 0.46826884249041 2307 12-13 0.44813810237488383 2307 14-15 0.5723817871385819 2307 16-17 0.47268086137906806 2307 18-19 0.6083693922632492 2307 20-21 0.6323962565727115 2307 22-23 0.5119443722318024 2307 24-25 0.6646675592559887 2307 26-27 0.6189746355613579 2307 28-29 0.4649729263702582 2307 30-31 0.1722884088558061 2307 32-33 0.3006191639788014 2307 34-35 0.3207211332468063 2307 36-37 0.3043927990305946 2307 38-39 0.35160081348882954 2307 40-41 0.5146239756568249 2307 42-43 0.5423909522883861 2307 44-45 0.45464482791471994 2307 46-47 0.4545441659216749 2307 48-49 0.46181431279205754 2307 50-51 0.3861862434096537 2307 52-53 0.512561317502545 2307 54-55 0.45614792855130304 2307 56-57 0.6083459329530179 2307 58-59 0.6896799674559055 2307 60-61 0.6600968306421429 2307 62-63 0.778314243412165 2307 64-65 0.8061674889340402 2307 66-67 0.7652227783881713 2307 68-69 0.7643711743726769 2307 70-71 0.8021187464605077 2307 72-73 0.7718690818981031 2307 74-75 0.7263911672053034 2307 76-77 0.5859261976449446 2307 78-79 0.7750533877438777 2307 80-81 0.7804292780514999 2307 82-83 0.6332955526526618 2307 84-85 0.5779739056064237 2307 86-87 0.6571599660150866 2307 88-89 0.7552727151133389 2307 90-91 0.6603915303121894 2307 92-93 0.6651061445008501 2308 1 0.5685475317273045 2308 2 0.3964341357149621 2308 3 0.42675652763936256 2308 4 0.3806982864217261 2308 5 0.43365170773113704 2308 6 0.40682268256941967 2308 7 0.48127296002702735 2308 8 0.374678297737276 2308 9 0.36809184365169045 2308 10-11 0.3946352987173469 2308 12-13 0.30213425410514105 2308 14-15 0.560557437552248 2308 16-17 0.6108782768159386 2308 18-19 0.6593395715109054 2308 20-21 0.5592432124070683 2308 22-23 0.5085148788894926 2308 24-25 0.6725543090303248 2308 26-27 0.5913526179036452 2308 28-29 0.47273656355933724 2308 30-31 0.44088321622867 2308 32-33 0.5872617822977446 2308 34-35 0.4024217894021689 2308 36-37 0.5674285457444768 2308 38-39 0.5553194277912539 2308 40-41 0.5269576000806495 2308 42-43 0.537528672809465 2308 44-45 0.3729725464061815 2308 46-47 0.29429040310968446 2308 48-49 0.4567031042056868 2308 50-51 0.592245065053973 2308 52-53 0.5292450757211782 2308 54-55 0.45880402916712626 2308 56-57 0.6463368143429911 2308 58-59 0.7747676217472197 2308 60-61 0.7837649581023918 2308 62-63 0.6476681560422897 2308 64-65 0.8197044320565574 2308 66-67 0.8178632896392202 2308 68-69 0.7996245032194764 2308 70-71 0.8671898900455659 2308 72-73 0.9060777563948363 2308 74-75 0.8031620456042496 2308 76-77 0.8459989635921623 2308 78-79 0.9674010793161436 2308 80-81 1.0346833386303658 2308 82-83 1.0120579306750628 2308 84-85 0.8648322713870016 2308 86-87 0.9105960355990668 2308 88-89 1.0136261109910691 2308 90-91 1.0957705755353686 2308 92-93 0.9405775308393594 2309 1 0.5918575541841307 2309 2 0.3920869547561949 2309 3 0.37587264204529447 2309 4 0.2319739839460695 2309 5 0.31678681696320865 2309 6 0.31694010789539817 2309 7 0.33269087097907146 2309 8 0.2334326590241389 2309 9 0.21030794611969128 2309 10-11 0.034247017486706 2309 12-13 0.11370867021638986 2309 14-15 0.3182372160473008 2309 16-17 0.2703665956623382 2309 18-19 0.2820515007707698 2309 20-21 0.3275927708162243 2309 22-23 0.20243778181158234 2309 24-25 0.2845337701565853 2309 26-27 0.2605875259928325 2309 28-29 0.20295324265197223 2309 30-31 0.2779866460189879 2309 32-33 0.1255202914527871 2309 34-35 0.1819735341421591 2309 36-37 0.20333279058019116 2309 38-39 0.19912392276042823 2309 40-41 0.13303977922620902 2309 42-43 0.08984530730378282 2309 44-45 0.2091842492958378 2309 46-47 0.2821688552554704 2309 48-49 0.13395178540208974 2309 50-51 0.25878095881088115 2309 52-53 0.3380532421674758 2309 54-55 0.15730391823446155 2309 56-57 0.26519070813594325 2309 58-59 0.3482267698068533 2309 60-61 0.3161333930845842 2309 62-63 0.3119763866831313 2309 64-65 0.4429999966644189 2309 66-67 0.21568129474325204 2309 68-69 0.36062817920148404 2309 70-71 0.2132543706441723 2309 72-73 0.1980319564070001 2309 74-75 0.25242531472803265 2309 76-77 0.19412641171283695 2309 78-79 0.3103972721295065 2309 80-81 0.47870677076322465 2309 82-83 0.17417190469992505 2309 84-85 0.33173901530484784 2309 86-87 0.4154468339764108 2309 88-89 0.5431002310190891 2309 90-91 0.5419898269144241 2309 92-93 0.6217956025029885 2310 1 0.6129949495676783 2310 2 0.3867829620060448 2310 3 0.30683587036236304 2310 4 0.2354189436987184 2310 5 0.30415264669827025 2310 6 0.35700812857880493 2310 7 0.2354457299800785 2310 8 0.08808252308939046 2310 9 0.1835495901305677 2310 10-11 0.06598412500842699 2310 12-13 0.11169904283752885 2310 14-15 0.08435509013440878 2310 16-17 0.04890315944504664 2310 18-19 -0.04545502942806223 2310 20-21 0.04746762555261341 2310 22-23 0.03156393992236417 2310 24-25 -0.08486493979596332 2310 26-27 0.08112867424166836 2310 28-29 -0.020751229867890686 2310 30-31 -4.34154663352615E-4 2310 32-33 -0.28358000173982134 2310 34-35 -0.1504995721001805 2310 36-37 -0.26185619603956667 2310 38-39 -0.14204583042471342 2310 40-41 -0.257869160482727 2310 42-43 -0.2794882285989857 2310 44-45 -0.2370718197910051 2310 46-47 -0.3059173433691882 2310 48-49 -0.5164295249022928 2310 50-51 -0.4490328692183283 2310 52-53 -0.4070619665323463 2310 54-55 -0.2885013698939005 2310 56-57 -0.33858360819222 2310 58-59 -0.28195514820583156 2310 60-61 -0.36867025316521307 2310 62-63 -0.5207252711877537 2310 64-65 -0.5186624693518915 2310 66-67 -0.6700728605955213 2310 68-69 -0.6124449803485987 2310 70-71 -0.591570673334715 2310 72-73 -0.55713304172723 2310 74-75 -0.6583935881985674 2310 76-77 -0.5611477500228617 2310 78-79 -0.4971601882894916 2310 80-81 -0.4637457693508509 2310 82-83 -0.47720615383197895 2310 84-85 -0.693727118284361 2310 86-87 -0.5873955136967055 2310 88-89 -0.7081727153000088 2310 90-91 -0.6635669831500799 2310 92-93 -0.6724353799587597 2311 1 0.5101900042514629 2311 2 0.2657840081306304 2311 3 0.16084138629199174 2311 4 0.09475893418849779 2311 5 0.17357480551900295 2311 6 0.1262368494537469 2311 7 -0.05240629094240745 2311 8 0.07208366431621016 2311 9 0.02098182978819807 2311 10-11 -0.03162823828211003 2311 12-13 0.0443458331371005 2311 14-15 0.09816945057187354 2311 16-17 0.0406930786081503 2311 18-19 -0.05947547640857209 2311 20-21 -0.09262785709123023 2311 22-23 -0.05709473815657873 2311 24-25 -0.053991520870624754 2311 26-27 0.10048017210186799 2311 28-29 0.07551001597138907 2311 30-31 0.06830983012029179 2311 32-33 0.16422351704287053 2311 34-35 0.1834143603772347 2311 36-37 -0.07080845471764974 2311 38-39 0.03966020856720576 2311 40-41 0.10845209486677732 2311 42-43 0.10418395399730684 2311 44-45 -0.0729828040991336 2311 46-47 -0.04257782839059132 2311 48-49 -0.06310047116953399 2311 50-51 0.09366004566517461 2311 52-53 0.31057255557892915 2311 54-55 0.4323088060452349 2311 56-57 0.2696954217649825 2311 58-59 0.26059558883649103 2311 60-61 0.37037739305447204 2311 62-63 0.44455138121357507 2311 64-65 0.3468893138150122 2311 66-67 0.22575091496320354 2311 68-69 0.3179258232652842 2311 70-71 0.24110693008896433 2311 72-73 0.2318319606503252 2311 74-75 0.01826860866306035 2311 76-77 -0.004159637802224836 2311 78-79 0.34970942654645754 2311 80-81 0.3490466224703539 2311 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0.04125507323026767 2302 88-89 0.010131382891842122 2302 90-91 0.060914923023979384 2302 92-93 0.15046090901524778 2303 1 -0.23919493726801733 2303 2 -0.14908032405612204 2303 3 -0.04034545036532222 2303 4 -0.3702272387966232 2303 5 -0.34366577473132054 2303 6 -0.24721390264880938 2303 7 -0.5676191031383837 2303 8 -0.7369455817666477 2303 9 -0.4260851326885131 2303 10-11 -0.6921554824976681 2303 12-13 -0.5282275579359919 2303 14-15 -1.240672384439435 2303 16-17 -0.8679528210735015 2303 18-19 -1.3624934193435436 2303 20-21 -1.044017347946891 2303 22-23 -2.0900674022922203 2303 24-25 -1.3035164796899608 2303 26-27 -1.0816556833002267 2303 28-29 -0.7623326938415289 2303 30-31 -0.43681974412044866 2303 32-33 -0.3075877943196801 2303 34-35 -0.46561316614114645 2303 36-37 -0.17116119532333585 2303 38-39 -0.015348996160277295 2303 40-41 -0.497687639210703 2303 42-43 -0.5048778289427744 2303 44-45 -0.26412476349534586 2303 46-47 -0.6816040644693189 2303 48-49 -0.5661606522479445 2303 50-51 -0.5917736799904247 2303 52-53 -0.6817767924885132 2303 54-55 -0.9177460912805238 2303 56-57 -1.1157592850293483 2303 58-59 -1.6093576120362307 2303 60-61 -1.6685886889194066 2303 62-63 -1.7984800914140848 2303 64-65 -1.8184610941907415 2303 66-67 -1.7379313622428505 2303 68-69 -1.8115633588044062 2303 70-71 -1.7063691262784175 2303 72-73 -1.641887117074603 2303 74-75 -1.3986748946016583 2303 76-77 -1.3436120101575142 2303 78-79 -1.6539430847226662 2303 80-81 -1.4223154871563253 2303 82-83 -1.43184603350538 2303 84-85 -1.4245224484662842 2303 86-87 -1.3002967599365007 2303 88-89 -1.1601842036254624 2303 90-91 -1.0999218320256006 2303 92-93 -1.3423306671668946 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 4.0 7 20.0 8 296.0 9 2510.0 10 15404.0 11 88888.0 12 69235.0 13 34208.0 14 12609.0 15 5276.0 16 6778.0 17 12071.0 18 20500.0 19 32637.0 20 46734.0 21 65874.0 22 90335.0 23 103894.0 24 125430.0 25 147615.0 26 170593.0 27 192843.0 28 214570.0 29 232473.0 30 248947.0 31 260995.0 32 272869.0 33 288446.0 34 317235.0 35 384444.0 36 551507.0 37 926003.0 38 916922.0 39 18051.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.20625959023265 24.723785958791446 10.82569154504976 26.244262905926146 2 15.454500744271357 22.879805153553722 36.2668192818611 25.398874820313814 3 14.850557765310574 26.997862740603352 30.46648889923568 27.685090594850394 4 11.436660695137705 17.610973182236123 39.57168361889971 31.380682503726465 5 11.872638467912264 37.54931106186174 37.216494897993044 13.361555572232952 6 28.64999709847339 41.43465089869894 17.66059694527959 12.25475505754808 7 26.16349940786734 32.43891435595384 25.0731040055192 16.324482230659623 8 23.35007369537238 36.53859277150588 22.502981084599156 17.60835244852258 9 22.46669923864282 14.688344559093036 25.324541282256934 37.52041492000721 10-11 22.48259381843795 26.966550078051917 30.607446933971293 19.943409169538835 12-13 21.554394604555956 25.741833564008953 30.892800589222624 21.810971242212464 14-15 19.581450106420508 27.102011379089642 26.493048163469794 26.82349035102005 16-17 19.930816905262656 31.616059946769536 27.49183499764684 20.961288150320964 18-19 20.15833486067652 30.31189964563936 29.000681901298066 20.529083592386055 20-21 20.352923982755556 29.943169664771073 28.748274563881843 20.95563178859153 22-23 20.353238301406417 29.743818840283737 28.69043485956242 21.21250799874743 24-25 20.145994580400803 29.563083525522394 28.906019188107766 21.38490270596904 26-27 20.4858235228367 30.140799747844145 28.57049255786151 20.80288417145765 28-29 20.459534117765088 30.039860128187573 28.53169291367828 20.96891284036906 30-31 20.36729385260674 29.692990076730684 28.743215471081633 21.196500599580943 32-33 20.52572277470767 30.005260302810704 28.62246250918653 20.84655441329509 34-35 20.316580515027397 29.73511577616178 28.727807703950447 21.22049600486037 36-37 20.4095533525673 29.872116399001087 28.716458057658517 21.001872190773096 38-39 20.378617635356484 29.909406724003567 28.728375817782187 20.98359982285776 40-41 20.326970090096665 29.741527325406874 28.929363296881977 21.00213928761448 42-43 20.284951101282168 29.87237998011887 28.848079450890584 20.994589467708384 44-45 20.24330708145682 29.656905221868747 28.870322551293654 21.22946514538078 46-47 20.14729901092335 29.619927113963122 29.065222510840282 21.167551364273244 48-49 20.153342253250276 29.892292268278975 28.913790471115618 21.040575007355127 50-51 20.12661798395828 29.864445566760235 28.949308920487542 21.059627528793946 52-53 20.146265786377814 29.71395533408015 29.045263206200396 21.09451567334164 54-55 20.152166489742726 29.810339438711697 28.974590247185983 21.062903824359598 56-57 20.02402631998503 29.78521452422304 28.945909965608426 21.24484919018351 58-59 20.090342203148886 29.78580918861783 28.82631977262107 21.297528835612216 60-61 19.967429151383524 29.728854706003776 29.0160845507677 21.287631591845 62-63 20.00849291930143 29.810746227151235 28.883378850501444 21.297382003045893 64-65 20.00836443347579 29.742511959939577 28.865464653326566 21.38365895325806 66-67 19.947771093184958 29.62545028816026 28.84906037461505 21.577718244039737 68-69 19.9850472619724 29.828706247837154 28.721750466243133 21.46449602394731 70-71 20.093879831235967 29.776374038662073 28.675758123041224 21.45398800706074 72-73 20.090496691912218 29.802858264513922 28.604544700946484 21.502100342627376 74-75 20.03920492427893 29.85746253176466 28.68523780367874 21.418094740277677 76-77 19.994495384787893 29.799157550653682 28.723244918804692 21.483102145753737 78-79 20.117916493793608 29.77781410882064 28.607074077376595 21.497195320009162 80-81 19.970168367100776 29.87037481460409 28.574158766481762 21.585298051813375 82-83 20.008258102726828 29.825010660159133 28.63614250395224 21.5305887331618 84-85 19.964934783434522 29.897865741247664 28.649908716919658 21.487290758398156 86-87 19.844907902112034 29.738290352650488 28.740921133510543 21.67588061172694 88-89 20.0132690825367 29.725929748295382 28.60423064728646 21.65657052188146 90-91 20.008620651809792 29.85960128007093 28.59699205371575 21.534786014403533 92-93 20.06793414583524 29.757503665631884 28.496490589871026 21.678071598661848 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 329.0 1 240.0 2 237.5 3 565.0 4 1475.5 5 3343.5 6 6536.5 7 11187.5 8 16518.5 9 21319.0 10 25002.0 11 27108.5 12 26984.0 13 24971.0 14 23632.0 15 22144.5 16 19428.0 17 17611.5 18 17231.5 19 18294.5 20 21273.0 21 26313.0 22 33468.0 23 43474.0 24 55647.5 25 69721.0 26 86485.0 27 105788.0 28 126599.0 29 137203.0 30 147018.5 31 166259.0 32 184203.0 33 200805.0 34 215122.5 35 225669.0 36 232971.5 37 237479.0 38 238211.0 39 234738.0 40 230041.0 41 224007.5 42 216105.0 43 212227.0 44 206573.5 45 194410.5 46 182801.0 47 170916.0 48 158775.5 49 146528.0 50 133920.0 51 122365.0 52 111101.5 53 99917.0 54 89083.0 55 79035.5 56 70015.5 57 65969.0 58 62605.5 59 56348.5 60 50533.5 61 45664.5 62 41200.5 63 36249.5 64 31590.0 65 27842.0 66 25228.5 67 22710.5 68 19328.0 69 15971.0 70 13500.0 71 12431.0 72 11637.5 73 10144.5 74 8618.0 75 7707.5 76 7715.5 77 7041.5 78 5747.5 79 4719.0 80 3577.0 81 2919.5 82 2596.0 83 2207.0 84 1897.5 85 1291.0 86 913.0 87 781.0 88 577.5 89 357.0 90 152.0 91 89.5 92 75.5 93 42.0 94 15.0 95 18.0 96 23.5 97 22.0 98 34.0 99 130.0 100 211.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.16287689852161688 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0011997514727587492 18-19 0.0 20-21 0.03365260336709825 22-23 0.005054272161834732 24-25 0.2629497855508059 26-27 1.9519531018000187 28-29 2.63495204482748 30-31 3.378049176876892 32-33 3.70642370763367 34-35 3.811533849073307 36-37 3.8985881562917095 38-39 3.893287126734769 40-41 3.933032085098287 42-43 3.8654545943419043 44-45 3.8668415410799155 46-47 3.8184430561362865 48-49 3.8068369496905916 50-51 3.8324486655274983 52-53 4.324602035629385 54-55 4.662829844439033 56-57 4.734159749376172 58-59 4.769599216638868 60-61 4.860865417325466 62-63 4.901665476274957 64-65 5.00745632777006 66-67 4.929285286775489 68-69 4.991187357444423 70-71 5.019096469718528 72-73 5.001746872179839 74-75 4.999049558585056 76-77 4.8423245771897125 78-79 4.861129192471959 80-81 4.944260907995739 82-83 4.8353132636184135 84-85 4.805038683901564 86-87 4.803268837757353 88-89 4.8889447071134375 90-91 5.027596836536159 92-93 4.683361761487024 >>END_MODULE >>Sequence Length Distribution pass #Length Count 93 5876217.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.50917233372371 #Duplication Level Percentage of deduplicated Percentage of total 1 94.25925072674625 82.4854901589451 2 3.538381697460387 6.192817074911097 3 0.8709213555739288 2.286408209821176 4 0.5464829072585293 1.9128906763488402 5 0.22589062200782906 0.9883750684927577 6 0.1430661921273862 0.7511762437203039 7 0.09407898995469749 0.57629421814498 8 0.05193218964787001 0.36356343468504637 9 0.05415776492034455 0.42653710652613547 >10 0.2043513836947234 3.112597246833632 >50 0.009780942116802398 0.5478361954397759 >100 0.0015984344716844195 0.24281027677091838 >500 6.528412608668656E-5 0.03790515773077855 >1k 4.150989342554682E-5 0.07529893162942305 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.701775138664893E-5 1.701775138664893E-5 0.0 2 1.701775138664893E-5 1.701775138664893E-5 0.0 3 1.701775138664893E-5 3.403550277329786E-5 0.0 4 1.701775138664893E-5 3.403550277329786E-5 0.0 5 1.701775138664893E-5 3.403550277329786E-5 0.0 6 1.701775138664893E-5 3.403550277329786E-5 0.0 7 1.701775138664893E-5 3.403550277329786E-5 0.0 8 1.701775138664893E-5 3.403550277329786E-5 0.0 9 1.701775138664893E-5 3.403550277329786E-5 0.0 10-11 4.254437846662232E-5 3.403550277329786E-5 8.508875693324464E-5 12-13 5.1053254159946785E-5 3.403550277329786E-5 1.0210650831989357E-4 14-15 5.956212985327125E-5 3.403550277329786E-5 1.191242597065425E-4 16-17 6.807100554659571E-5 3.403550277329786E-5 1.7868638955981375E-4 18-19 8.508875693324464E-5 3.403550277329786E-5 2.978106492663562E-4 20-21 1.1912425970654249E-4 3.403550277329786E-5 3.7439053050627644E-4 22-23 1.1912425970654249E-4 4.254437846662232E-5 5.70094671452739E-4 24-25 1.5315976247984035E-4 8.508875693324464E-5 0.0013444023595452653 26-27 2.0421301663978714E-4 8.508875693324464E-5 0.005547786952047551 28-29 2.1272189233311162E-4 9.359763262656911E-5 0.026947609320758577 30-31 2.3824851941308498E-4 1.1061538401321803E-4 0.04517362105585958 32-33 2.3824851941308498E-4 1.1912425970654249E-4 0.06466745526926593 34-35 2.3824851941308498E-4 1.1912425970654249E-4 0.09782654384615136 36-37 2.3824851941308498E-4 1.1912425970654249E-4 0.1689692535180372 38-39 2.3824851941308498E-4 1.1912425970654249E-4 0.2861705073178884 40-41 2.3824851941308498E-4 1.1912425970654249E-4 0.4196832758218425 42-43 2.3824851941308498E-4 1.1912425970654249E-4 0.5542341271603823 44-45 2.4675739510640946E-4 1.2763313539986695E-4 0.7104826115168994 46-47 2.7228402218638286E-4 1.3614201109319143E-4 0.8929894862630159 48-49 3.063195249596807E-4 1.3614201109319143E-4 1.0775555089269166 50-51 3.1482840065300515E-4 1.3614201109319143E-4 1.2610068688749922 52-53 3.573727791196275E-4 1.4465088678651587E-4 1.430648323572802 54-55 3.743905305062764E-4 1.5315976247984035E-4 1.6079818018973773 56-57 3.743905305062764E-4 1.5315976247984035E-4 1.8062386055518371 58-59 3.999171575862498E-4 1.5315976247984035E-4 2.0042231251841107 60-61 4.8500591451949447E-4 1.7017751386648927E-4 2.196345029463684 62-63 5.020236659061434E-4 1.7017751386648927E-4 2.380638768105398 64-65 5.445680443727657E-4 1.7017751386648927E-4 2.5587465541180663 66-67 5.445680443727657E-4 1.9570414094646267E-4 2.7500686240824663 68-69 5.615857957594146E-4 2.3824851941308498E-4 2.9492699129388855 70-71 5.956212985327125E-4 2.552662707997339E-4 3.144565627852069 72-73 5.956212985327125E-4 2.7228402218638286E-4 3.330501579502595 74-75 5.956212985327125E-4 2.7228402218638286E-4 3.5076308448105307 76-77 6.04130174226037E-4 2.7228402218638286E-4 3.692545731377858 78-79 6.211479256126858E-4 2.807928978797073E-4 3.88494842855531 80-81 6.296568013060103E-4 3.1482840065300515E-4 4.074832498527539 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGTGCG 760 0.0 15.4491005 9 TACGCAG 530 0.0 15.032684 5 TACCCCG 1185 0.0 14.154692 5 AGTGCGC 940 0.0 13.829059 8 TATACAG 2325 0.0 13.707221 5 TTATACT 4120 0.0 12.722474 4 TATACTG 2310 0.0 12.707052 5 ACTGTGC 1585 0.0 12.434473 8 TAGACTG 1695 0.0 12.369697 5 GTATAGA 2675 0.0 12.249056 1 TCTATAC 2945 0.0 12.24537 3 CTTACAC 1755 0.0 12.185738 3 ACAGTGC 1480 0.0 12.183316 8 CCTATAC 2220 0.0 12.088871 3 TATACTA 3645 0.0 12.079544 5 CTATACC 2375 0.0 12.006158 4 GACTGTG 1410 0.0 11.895964 7 GTGTAAG 1545 0.0 11.691541 1 GTATAAG 2485 0.0 11.6641865 1 GTATAGG 1770 0.0 11.629328 1 >>END_MODULE