FastQCFastQC Report
Wed 5 Nov 2014
lane3_Undetermined_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane3_Undetermined_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5876217
Sequences flagged as poor quality0
Sequence length93
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTGCG7600.015.44910059
TACGCAG5300.015.0326845
TACCCCG11850.014.1546925
AGTGCGC9400.013.8290598
TATACAG23250.013.7072215
TTATACT41200.012.7224744
TATACTG23100.012.7070525
ACTGTGC15850.012.4344738
TAGACTG16950.012.3696975
GTATAGA26750.012.2490561
TCTATAC29450.012.245373
CTTACAC17550.012.1857383
ACAGTGC14800.012.1833168
CCTATAC22200.012.0888713
TATACTA36450.012.0795445
CTATACC23750.012.0061584
GACTGTG14100.011.8959647
GTGTAAG15450.011.6915411
GTATAAG24850.011.66418651
GTATAGG17700.011.6293281
Produced by FastQC (version 0.11.2)