##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename lane2_Undetermined_L002_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5754556 Sequences flagged as poor quality 0 Sequence length 93 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.38781688804488 33.0 27.0 33.0 15.0 33.0 2 28.317818264345675 33.0 27.0 33.0 15.0 33.0 3 28.379503127608803 33.0 27.0 33.0 15.0 33.0 4 31.682417548808285 37.0 33.0 37.0 15.0 37.0 5 31.690055670672074 37.0 33.0 37.0 15.0 37.0 6 31.715663380458892 37.0 33.0 37.0 15.0 37.0 7 31.60160836040174 37.0 33.0 37.0 15.0 37.0 8 31.545826472103148 37.0 33.0 37.0 15.0 37.0 9 31.653159861507994 37.0 33.0 37.0 15.0 37.0 10-11 31.613638046097734 37.0 33.0 37.0 15.0 37.0 12-13 31.57574494018305 37.0 33.0 37.0 15.0 37.0 14-15 32.81547829233046 37.0 33.0 40.0 15.0 40.0 16-17 32.764341245441 37.0 33.0 40.0 15.0 40.0 18-19 32.75338896693333 37.0 33.0 40.0 15.0 40.0 20-21 32.59577393981395 37.0 33.0 40.0 15.0 40.0 22-23 32.42776297597938 37.0 30.0 40.0 15.0 40.0 24-25 32.240744029600194 37.0 27.0 40.0 15.0 40.0 26-27 32.07740996872739 37.0 27.0 40.0 15.0 40.0 28-29 31.910797635821076 37.0 27.0 40.0 15.0 40.0 30-31 31.72145844092924 37.0 27.0 40.0 15.0 40.0 32-33 31.57927397005086 37.0 27.0 40.0 15.0 40.0 34-35 31.513851546496376 37.0 27.0 40.0 15.0 40.0 36-37 31.441850943843452 37.0 27.0 40.0 6.0 40.0 38-39 31.33064818554203 37.0 27.0 40.0 6.0 40.0 40-41 31.307035156144103 37.0 27.0 40.0 6.0 40.0 42-43 31.35677583118489 37.0 27.0 40.0 10.5 40.0 44-45 31.27367211301793 37.0 27.0 40.0 10.5 40.0 46-47 31.13862555164986 37.0 27.0 40.0 6.0 40.0 48-49 30.861632330973926 37.0 27.0 40.0 6.0 40.0 50-51 29.76301499194725 35.0 24.5 38.5 6.0 38.5 52-53 29.63632059536826 35.0 22.0 37.0 6.0 40.0 54-55 30.134594137236654 37.0 27.0 40.0 6.0 40.0 56-57 30.05549585406763 37.0 27.0 40.0 6.0 40.0 58-59 29.914922193823468 37.0 24.5 40.0 6.0 40.0 60-61 30.144844623981413 37.0 27.0 40.0 6.0 40.0 62-63 30.162306874761494 37.0 27.0 40.0 6.0 40.0 64-65 30.053009302542193 37.0 27.0 37.0 6.0 40.0 66-67 29.921028138400253 33.0 27.0 37.0 6.0 40.0 68-69 29.77909859596466 33.0 27.0 37.0 6.0 40.0 70-71 29.669611261059934 33.0 27.0 37.0 6.0 40.0 72-73 29.351013006042514 33.0 22.0 37.0 6.0 40.0 74-75 29.214465460063295 33.0 22.0 37.0 6.0 40.0 76-77 28.800191969632408 33.0 22.0 37.0 6.0 37.0 78-79 28.38775693554811 33.0 22.0 37.0 6.0 37.0 80-81 27.9839477624338 33.0 22.0 37.0 6.0 37.0 82-83 27.772924270786486 33.0 22.0 37.0 6.0 37.0 84-85 27.652042138437785 33.0 22.0 37.0 6.0 37.0 86-87 27.61045326520413 33.0 22.0 37.0 6.0 37.0 88-89 27.54233063332775 33.0 22.0 37.0 6.0 37.0 90-91 27.43735746076674 33.0 22.0 37.0 6.0 37.0 92-93 26.687773044523333 33.0 18.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2304 1 -0.18879838660389225 2304 2 0.039626217546963716 2304 3 0.03507913055321055 2304 4 0.05350989786461113 2304 5 -0.20440845513925865 2304 6 0.0860144447686082 2304 7 -0.06203019394177289 2304 8 0.23907968218554743 2304 9 0.14896905876375754 2304 10-11 0.1727445413048585 2304 12-13 0.13222486701670277 2304 14-15 0.3994061478868076 2304 16-17 0.5902835325225979 2304 18-19 0.5426157850066602 2304 20-21 0.5325006229499749 2304 22-23 0.5144353946651705 2304 24-25 0.3807969341015749 2304 26-27 0.34942930296386265 2304 28-29 0.1669848793177806 2304 30-31 0.18580174359226476 2304 32-33 0.2677850324697175 2304 34-35 0.23035047910173745 2304 36-37 0.2061825943651563 2304 38-39 0.3448003146627201 2304 40-41 0.33121094620519287 2304 42-43 0.13759982972947782 2304 44-45 0.47015515399974106 2304 46-47 0.4218749548931271 2304 48-49 0.391411664136907 2304 50-51 0.29783062169816077 2304 52-53 0.16625870377678353 2304 54-55 0.35551386079042757 2304 56-57 0.31891576107673814 2304 58-59 0.43089191079073075 2304 60-61 0.2832568979976955 2304 62-63 0.1441185150965083 2304 64-65 0.3792440645657358 2304 66-67 0.3486325666052785 2304 68-69 0.34954547233382627 2304 70-71 0.34566473736503767 2304 72-73 0.23844322377145843 2304 74-75 0.28966848257850586 2304 76-77 0.19996513367119917 2304 78-79 0.005829926726491408 2304 80-81 0.03845549156069694 2304 82-83 0.01626609679275859 2304 84-85 -0.013769834682886284 2304 86-87 0.07949786976411133 2304 88-89 0.22169246522307162 2304 90-91 -0.010595170085963446 2304 92-93 0.14161313113181606 2305 1 0.4206653679444088 2305 2 0.6607223359188268 2305 3 0.8126285286472879 2305 4 0.6201433863490955 2305 5 0.588343043563885 2305 6 0.7035821963426585 2305 7 0.9087485536106144 2305 8 0.9491561372104833 2305 9 1.0674140869542548 2305 10-11 1.102011417177632 2305 12-13 1.1034129308711442 2305 14-15 1.4683589241828443 2305 16-17 1.7431479315659928 2305 18-19 1.4955991707100154 2305 20-21 1.7143845615043034 2305 22-23 1.4336282333285197 2305 24-25 1.4065333472798116 2305 26-27 1.2353545756153679 2305 28-29 1.3418833516145412 2305 30-31 1.256944115528178 2305 32-33 1.1577503172966033 2305 34-35 1.2783191510621137 2305 36-37 1.2503440630709513 2305 38-39 1.1648042585102232 2305 40-41 1.4274448321155369 2305 42-43 1.3047379704197652 2305 44-45 1.5360596334378975 2305 46-47 1.3016545925851908 2305 48-49 1.3513601735707717 2305 50-51 1.29463547508265 2305 52-53 1.249963819052475 2305 54-55 1.3738361762823388 2305 56-57 1.3060669894776993 2305 58-59 1.142809467254601 2305 60-61 1.238226317083619 2305 62-63 1.195788944788216 2305 64-65 1.3696921522175032 2305 66-67 1.15186111743105 2305 68-69 1.1310900537010546 2305 70-71 1.2933308467697486 2305 72-73 1.0557985979931317 2305 74-75 1.0127025451469152 2305 76-77 1.074319720767214 2305 78-79 0.9456027589185894 2305 80-81 0.9103268519949381 2305 82-83 0.6024493017284449 2305 84-85 0.8047829746543478 2305 86-87 0.7793586433171242 2305 88-89 0.7563423299212957 2305 90-91 0.9468207974690053 2305 92-93 0.9223760320611092 2306 1 0.7543375229253222 2306 2 0.5833231089256081 2306 3 0.8292250960362963 2306 4 1.0810706894839974 2306 5 0.9516882210826374 2306 6 0.9390265735740329 2306 7 1.0571189529748608 2306 8 1.0925395075674835 2306 9 1.0961653302548306 2306 10-11 1.077334234772092 2306 12-13 1.1274981751172248 2306 14-15 1.5100620807683924 2306 16-17 1.6179567894577929 2306 18-19 1.628270110772334 2306 20-21 1.5411033832535495 2306 22-23 1.490557522355445 2306 24-25 1.3995394150949707 2306 26-27 1.140878134361003 2306 28-29 1.291769230600515 2306 30-31 1.406357379321399 2306 32-33 1.4937680954358683 2306 34-35 1.3165754294123317 2306 36-37 1.2923754679540131 2306 38-39 1.4854888490386031 2306 40-41 1.4474056564979527 2306 42-43 1.5582242106618693 2306 44-45 1.3485304254055386 2306 46-47 1.3996385055041607 2306 48-49 1.4248846744290127 2306 50-51 1.313940548426796 2306 52-53 1.4056644644673852 2306 54-55 1.3511493931393765 2306 56-57 1.2823995620042723 2306 58-59 1.2761198916627698 2306 60-61 1.2876840431694596 2306 62-63 1.3564254807960658 2306 64-65 1.4560433128290633 2306 66-67 1.5437270256631805 2306 68-69 1.618885866044426 2306 70-71 1.4388125526713367 2306 72-73 1.4153655311963362 2306 74-75 1.3267919746773984 2306 76-77 1.2623230351057941 2306 78-79 1.1512743106763281 2306 80-81 1.0835144628753213 2306 82-83 1.1086666687948288 2306 84-85 0.9639584905711374 2306 86-87 0.9975904199618846 2306 88-89 1.224134855037235 2306 90-91 1.2091001338557312 2306 92-93 1.1410844227938384 2307 1 -0.08109355649883909 2307 2 -0.1396479505194641 2307 3 0.007679017442171698 2307 4 -0.05471669340097307 2307 5 -0.12938680534742275 2307 6 -0.0069150793315309045 2307 7 -0.17724205110542002 2307 8 0.127882748897985 2307 9 0.12579012529442934 2307 10-11 0.12503818311770587 2307 12-13 0.18936065724234652 2307 14-15 0.4574906322261967 2307 16-17 0.37683595091854016 2307 18-19 0.39301884314865276 2307 20-21 0.3722786837407952 2307 22-23 0.3985085881495536 2307 24-25 0.29591050191663015 2307 26-27 0.4112132646473725 2307 28-29 0.32304844564949065 2307 30-31 0.3115761445205081 2307 32-33 0.32817194097361835 2307 34-35 0.23362166964486164 2307 36-37 0.35218359880247974 2307 38-39 0.11616114829389801 2307 40-41 0.10000535535541388 2307 42-43 0.349770085387739 2307 44-45 0.3077360964378997 2307 46-47 0.38299079858516194 2307 48-49 0.3275501936086478 2307 50-51 0.3108533423247266 2307 52-53 0.2982115361911504 2307 54-55 0.29936743897478735 2307 56-57 0.4038060782161672 2307 58-59 0.36891099368718017 2307 60-61 0.22412983314438506 2307 62-63 0.26055695834580206 2307 64-65 0.3625888526285159 2307 66-67 0.41871084726098573 2307 68-69 0.5595915996934657 2307 70-71 0.4030160248389514 2307 72-73 0.5248491911557345 2307 74-75 0.3892411022668938 2307 76-77 0.3794393768938349 2307 78-79 0.25026160968406685 2307 80-81 0.13624044355443488 2307 82-83 0.3373786582868803 2307 84-85 0.38453724342165074 2307 86-87 0.38504783333971915 2307 88-89 0.4495261510277295 2307 90-91 0.408583531698806 2307 92-93 0.367209848368816 2308 1 0.507436145298751 2308 2 0.5484101804956829 2308 3 0.5169616899704224 2308 4 0.5712301667419304 2308 5 0.6908653968771112 2308 6 0.9073345116248888 2308 7 0.6926738263963585 2308 8 0.8186458011068218 2308 9 0.8326780335805246 2308 10-11 0.7629519782157921 2308 12-13 0.8230545549181016 2308 14-15 0.9970273800405067 2308 16-17 0.9584243070866947 2308 18-19 0.8380362240577455 2308 20-21 0.9003567022620658 2308 22-23 0.897724932264012 2308 24-25 1.0573182729183372 2308 26-27 0.7545110098118499 2308 28-29 0.7148543524040747 2308 30-31 0.8168661660607128 2308 32-33 0.6309729942952771 2308 34-35 0.7157821359270002 2308 36-37 0.8617403443029978 2308 38-39 0.906894727328833 2308 40-41 0.876652409966173 2308 42-43 0.8672556655685426 2308 44-45 0.8467673930922821 2308 46-47 0.7390363800881268 2308 48-49 0.7205611950905677 2308 50-51 0.5974813357800315 2308 52-53 0.6371516588215869 2308 54-55 0.8399825744136606 2308 56-57 0.7773620304200435 2308 58-59 0.7731245557543822 2308 60-61 0.6798333054699022 2308 62-63 0.7966271650987409 2308 64-65 0.8718260758699046 2308 66-67 0.9172537155792497 2308 68-69 0.9748341887052021 2308 70-71 0.774014421718654 2308 72-73 0.8514491437782965 2308 74-75 0.7202275996969405 2308 76-77 0.621207546967824 2308 78-79 0.5369777472108552 2308 80-81 0.5818032159058433 2308 82-83 0.6467120136562308 2308 84-85 0.6576667986797204 2308 86-87 0.6744082930110551 2308 88-89 0.7679025372309809 2308 90-91 0.5981043034001523 2308 92-93 0.7386682471215806 2309 1 0.7452454244574263 2309 2 0.842023745868282 2309 3 0.8052622797167928 2309 4 0.8796535781051027 2309 5 0.9285090164002519 2309 6 0.7936521545690383 2309 7 0.9239545529059967 2309 8 0.9855524185676536 2309 9 0.9351117926407859 2309 10-11 0.9143175365452088 2309 12-13 1.0053601134887131 2309 14-15 1.205533934788022 2309 16-17 1.2730210001653148 2309 18-19 1.350229139865121 2309 20-21 1.2672111470683163 2309 22-23 1.0045764584890264 2309 24-25 1.1763650305193138 2309 26-27 0.9261547740580198 2309 28-29 0.8477623447337592 2309 30-31 0.8544083347354174 2309 32-33 0.7097219601285829 2309 34-35 0.8259987380713127 2309 36-37 0.8686453939309438 2309 38-39 0.8832633369022602 2309 40-41 0.8667790426229267 2309 42-43 0.9081784196479248 2309 44-45 0.9800594321192584 2309 46-47 0.9863308794067258 2309 48-49 0.6959481965498604 2309 50-51 0.6559691247738471 2309 52-53 0.8768622609900234 2309 54-55 0.6532819460744754 2309 56-57 0.5723310476300227 2309 58-59 0.5672963419984569 2309 60-61 0.5797949056242722 2309 62-63 0.5721231912453675 2309 64-65 0.6742729564854564 2309 66-67 0.9315652077943568 2309 68-69 0.9035854701070853 2309 70-71 0.9610711169681174 2309 72-73 0.9550405182853368 2309 74-75 0.7258313962645957 2309 76-77 0.7045361526819676 2309 78-79 0.5885488846133207 2309 80-81 0.6366210216634087 2309 82-83 0.686402595804644 2309 84-85 0.7113587315764747 2309 86-87 0.7986900928832128 2309 88-89 0.7707287610451559 2309 90-91 0.6492350968441976 2309 92-93 0.6461868500618664 2310 1 0.9049708511072545 2310 2 0.8548940085512591 2310 3 0.9755989930091928 2310 4 1.0255897256021065 2310 5 1.2187573268723462 2310 6 1.159058427093818 2310 7 1.2056874476214503 2310 8 1.167641677668346 2310 9 1.1973883061705557 2310 10-11 1.1267666827036003 2310 12-13 1.1400787061539717 2310 14-15 1.3299448843379338 2310 16-17 1.4113409130198207 2310 18-19 1.4614859752045746 2310 20-21 1.402197029280991 2310 22-23 1.4996389940544717 2310 24-25 1.407688092101047 2310 26-27 1.182554646157918 2310 28-29 1.298442632400608 2310 30-31 1.1050552166283367 2310 32-33 1.0984879083507195 2310 34-35 1.3106422707156469 2310 36-37 1.4587668881944005 2310 38-39 1.3838963774021522 2310 40-41 1.3075707226125282 2310 42-43 1.2826604838255946 2310 44-45 1.3002187002705092 2310 46-47 1.2997588949498429 2310 48-49 1.3485972570802112 2310 50-51 1.1794462517905728 2310 52-53 1.1457706769255083 2310 54-55 1.1591913212607743 2310 56-57 1.2292109786940344 2310 58-59 1.1813130882413923 2310 60-61 1.2745113738470586 2310 62-63 1.2954154917672653 2310 64-65 1.3410670752482936 2310 66-67 1.2456211478330523 2310 68-69 1.2997092297199195 2310 70-71 1.3099768665115263 2310 72-73 1.326720659625792 2310 74-75 1.2013770040339864 2310 76-77 0.983362961845252 2310 78-79 1.059849574515173 2310 80-81 1.0536935399406602 2310 82-83 1.1605631537959624 2310 84-85 1.179561095560672 2310 86-87 1.0453071458696286 2310 88-89 1.0086080905450956 2310 90-91 1.2728192047138513 2310 92-93 1.2387245291084703 2311 1 0.5888147947960256 2311 2 0.589928354080584 2311 3 0.6903916479230858 2311 4 0.8114799111622055 2311 5 0.8755467437456694 2311 6 0.8292539747862406 2311 7 0.917752012549613 2311 8 0.8069515433513992 2311 9 0.9611502032945829 2311 10-11 0.812541826461807 2311 12-13 0.8921535556667735 2311 14-15 1.0782305022538168 2311 16-17 0.883720795172799 2311 18-19 0.9083781980141836 2311 20-21 0.9708911261329192 2311 22-23 1.0258124421865489 2311 24-25 0.9079653775042758 2311 26-27 0.8228710556973766 2311 28-29 0.7435040057061855 2311 30-31 0.44635669406839185 2311 32-33 0.6191108304405262 2311 34-35 0.5733793408365244 2311 36-37 0.840671369341532 2311 38-39 0.6182068936919762 2311 40-41 0.49483267990733637 2311 42-43 0.5552495273344178 2311 44-45 0.4769541493216707 2311 46-47 0.6615297124517134 2311 48-49 0.630625815535172 2311 50-51 0.5651583915015728 2311 52-53 0.4469597753952286 2311 54-55 0.608025278971656 2311 56-57 0.7634722113865706 2311 58-59 0.5419493618822457 2311 60-61 0.5537034198404776 2311 62-63 0.49558641132396986 2311 64-65 0.578183632088443 2311 66-67 0.6726661968953458 2311 68-69 0.559828340196109 2311 70-71 0.6671202860942103 2311 72-73 0.5576162903995687 2311 74-75 0.5085160712569632 2311 76-77 0.7795179747407133 2311 78-79 0.3720403093355138 2311 80-81 0.41679724737809565 2311 82-83 0.32273653490781484 2311 84-85 0.3954617415256365 2311 86-87 0.2891347860971365 2311 88-89 0.49486687656788675 2311 90-91 0.40475964990721636 2311 92-93 0.2751875236779284 2313 1 -0.44684777106227 2313 2 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1.0316681635022356 1115 9 0.3308851646551396 1115 10-11 0.6441068243480679 1115 12-13 0.5068025104890239 1115 14-15 0.65972758452601 1115 16-17 0.39819640637339404 1115 18-19 0.7521285997832479 1115 20-21 0.8322079937240545 1115 22-23 0.5834144683274616 1115 24-25 0.7734794338492392 1115 26-27 0.9322077961085 1115 28-29 0.7558003453988249 1115 30-31 1.0068207233650313 1115 32-33 0.8003518721789433 1115 34-35 0.5579053073474185 1115 36-37 -0.34101292115121495 1115 38-39 0.24574184256108111 1115 40-41 0.6650262418798896 1115 42-43 0.6861556467689738 1115 44-45 0.9384283848649595 1115 46-47 0.8757679099884363 1115 48-49 0.7606682598460814 1115 50-51 0.8832591521792708 1115 52-53 1.0819567122507863 1115 54-55 1.1926965936452554 1115 56-57 1.2837783523806792 1115 58-59 1.3426671867507167 1115 60-61 1.3379307402284972 1115 62-63 1.1009234526862741 1115 64-65 1.1194251082129192 1115 66-67 0.8349601326300977 1115 68-69 0.9396334697401549 1115 70-71 -0.011751183482523686 1115 72-73 0.6203706791632264 1115 74-75 0.29298272238919054 1115 76-77 0.951827533158756 1115 78-79 1.0981752075845748 1115 80-81 1.1328466864457134 1115 82-83 1.2871714614033785 1115 84-85 0.6398615466528987 1115 86-87 0.9764395453479793 1115 88-89 0.9110619376668332 1115 90-91 0.9464237585648938 1115 92-93 0.734461110745972 1116 1 0.727960510478816 1116 2 0.9007064703430601 1116 3 0.7776654174992963 1116 4 1.0686017844406983 1116 5 0.7854495410276492 1116 6 0.8171949265662448 1116 7 0.4724179870346319 1116 8 0.6620501961525456 1116 9 0.7850544691591388 1116 10-11 0.43696633709204136 1116 12-13 0.5189534097154933 1116 14-15 0.5386938003942987 1116 16-17 0.06526771175044388 1116 18-19 0.21028932047930482 1116 20-21 0.547037766140221 1116 22-23 0.7348127903648702 1116 24-25 0.8337333583011244 1116 26-27 0.952728609234029 1116 28-29 0.8819141344198727 1116 30-31 0.8731563609631721 1116 32-33 0.5564155200072065 1116 34-35 0.2704845084663532 1116 36-37 0.3444198468262307 1116 38-39 0.5753759585564318 1116 40-41 0.6446451624561327 1116 42-43 0.7871170997973636 1116 44-45 0.6880133708030307 1116 46-47 0.892833915562111 1116 48-49 0.9328533361334408 1116 50-51 1.0571127155746005 1116 52-53 0.9595048120115379 1116 54-55 0.8272576929976374 1116 56-57 0.6657878958873553 1116 58-59 0.7830920317066301 1116 60-61 0.8627835819762559 1116 62-63 0.8447662847604 1116 64-65 0.5322030760871961 1116 66-67 0.5508369355638756 1116 68-69 0.7928894509245161 1116 70-71 0.7767678413922603 1116 72-73 0.8775915832001076 1116 74-75 0.7496027388637145 1116 76-77 0.7565112847791404 1116 78-79 0.8462247266536735 1116 80-81 0.7173314567070435 1116 82-83 1.001909331765816 1116 84-85 0.9816374213461287 1116 86-87 0.9103118519365268 1116 88-89 0.85944534434865 1116 90-91 0.9364444161594996 1116 92-93 0.9079954511157524 2301 1 -1.7475616701707821 2301 2 -1.979543063404428 2301 3 -2.0995360057839108 2301 4 -2.033383403558684 2301 5 -1.9288644760853693 2301 6 -2.248795685769558 2301 7 -2.227149798684671 2301 8 -2.077773957455168 2301 9 -2.6398478053015992 2301 10-11 -2.5714012651337974 2301 12-13 -2.7215133184946545 2301 14-15 -2.7717200913005513 2301 16-17 -2.924552198144923 2301 18-19 -2.7818983612306383 2301 20-21 -2.6665709426524806 2301 22-23 -2.778601194937952 2301 24-25 -2.965891891610262 2301 26-27 -2.628883976960431 2301 28-29 -2.418935474765174 2301 30-31 -2.6464126780991286 2301 32-33 -2.5582548930553166 2301 34-35 -2.622327984831074 2301 36-37 -2.664283038630561 2301 38-39 -2.645190241738561 2301 40-41 -2.7131199051757307 2301 42-43 -2.817922282984867 2301 44-45 -2.5157358928047096 2301 46-47 -2.3222697116330266 2301 48-49 -2.289594196704005 2301 50-51 -2.0782855063372168 2301 52-53 -2.287067975883005 2301 54-55 -2.2835197813319645 2301 56-57 -2.0832392089264005 2301 58-59 -1.97480061756184 2301 60-61 -2.146881950517514 2301 62-63 -2.007532575156791 2301 64-65 -2.0577909984255776 2301 66-67 -2.017799722950734 2301 68-69 -2.0226037217710946 2301 70-71 -2.1500580163438805 2301 72-73 -2.049158327062848 2301 74-75 -2.055487660330556 2301 76-77 -1.9184283177082264 2301 78-79 -1.6389502320538405 2301 80-81 -1.4986520801121301 2301 82-83 -1.4197341177669536 2301 84-85 -1.4549769133909507 2301 86-87 -1.3803768387384885 2301 88-89 -1.487825079234284 2301 90-91 -1.5275606287391064 2301 92-93 -1.949761435304385 2302 1 0.0713823195930594 2302 2 0.18958593720858374 2302 3 0.031436187631829426 2302 4 0.21223938821749755 2302 5 0.12378839087062232 2302 6 0.09990900289548676 2302 7 0.18426201599099912 2302 8 -0.42229399267648304 2302 9 -0.18978305741772417 2302 10-11 0.11872481369717391 2302 12-13 -0.07032754384818674 2302 14-15 -0.475315509120108 2302 16-17 -0.40929806588592044 2302 18-19 -0.17759407786276427 2302 20-21 -0.4225800429962092 2302 22-23 0.08226506566747105 2302 24-25 0.017795814697315393 2302 26-27 0.09372118691626952 2302 28-29 0.11409243639657518 2302 30-31 -0.44741364757766533 2302 32-33 -8.171090887927335E-4 2302 34-35 0.10392156499838734 2302 36-37 0.2054257042745924 2302 38-39 -0.794379417141549 2302 40-41 -0.1949921945611912 2302 42-43 0.05204966335056582 2302 44-45 0.23833422189698084 2302 46-47 -0.1349047092311828 2302 48-49 -0.034514798430880234 2302 50-51 0.29739319177181756 2302 52-53 0.10931093385031687 2302 54-55 0.29096368707814335 2302 56-57 0.2030258536109244 2302 58-59 0.2908633260032474 2302 60-61 0.24270101099460462 2302 62-63 -0.321621847695031 2302 64-65 -0.08407703839284508 2302 66-67 -0.10384256290272376 2302 68-69 -0.5747886867533474 2302 70-71 -1.0098418794094464 2302 72-73 -0.6589313775797514 2302 74-75 -0.09831227192409386 2302 76-77 9.627122379640696E-4 2302 78-79 0.3909130960741187 2302 80-81 0.23393801110029955 2302 82-83 0.28707354400103213 2302 84-85 0.19334015068939436 2302 86-87 0.21840194945093216 2302 88-89 -0.014324222109124207 2302 90-91 0.06735030082732507 2302 92-93 -0.10220273396340929 2303 1 -0.5459320603885907 2303 2 -0.46943299110976255 2303 3 -0.6898693650575538 2303 4 -0.8921150109747238 2303 5 -0.9330276498398149 2303 6 -1.0244145345910631 2303 7 -1.3117665207289768 2303 8 -1.146857768632028 2303 9 -0.9722394889033481 2303 10-11 -1.0597957256623296 2303 12-13 -1.0890214527525117 2303 14-15 -1.6254891364967818 2303 16-17 -1.606073099961499 2303 18-19 -1.5159620620987866 2303 20-21 -1.8088049551294425 2303 22-23 -2.024195446433545 2303 24-25 -1.5444773680566222 2303 26-27 -1.333992379552754 2303 28-29 -1.0363265082173427 2303 30-31 -0.7859687638440072 2303 32-33 -0.7440547149537338 2303 34-35 -0.6064583014046683 2303 36-37 -0.7732216349576113 2303 38-39 -0.6577573376681336 2303 40-41 -0.6562135927158117 2303 42-43 -0.5389778205354361 2303 44-45 -0.5418513426244864 2303 46-47 -0.5847102254088092 2303 48-49 -0.34195586506664455 2303 50-51 -0.2210651273000117 2303 52-53 -0.17856207380538436 2303 54-55 -0.21559749686393914 2303 56-57 -0.10667574693897564 2303 58-59 -0.14331902024282073 2303 60-61 -0.21884993853111823 2303 62-63 -0.09495528369966166 2303 64-65 -0.0989090192914368 2303 66-67 -0.5867865042276073 2303 68-69 -0.35935719489971873 2303 70-71 -0.41465941554459107 2303 72-73 -0.748874305276928 2303 74-75 -0.7210170988081828 2303 76-77 -0.514783564366116 2303 78-79 -0.2146648301776679 2303 80-81 -0.1663528704379047 2303 82-83 -0.1535902140521337 2303 84-85 -0.1992597836240506 2303 86-87 -0.24832357769653512 2303 88-89 -0.21686563576232842 2303 90-91 -0.21573780632155604 2303 92-93 -0.1850226181234902 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 96665.0 3 7878.0 4 6525.0 5 4151.0 6 4774.0 7 11713.0 8 21089.0 9 19140.0 10 22138.0 11 38966.0 12 67279.0 13 88152.0 14 94439.0 15 94146.0 16 94178.0 17 93872.0 18 92388.0 19 92312.0 20 91615.0 21 92977.0 22 95711.0 23 100190.0 24 106196.0 25 109920.0 26 118023.0 27 125554.0 28 133985.0 29 145168.0 30 160324.0 31 178564.0 32 200427.0 33 230553.0 34 269688.0 35 335297.0 36 468517.0 37 805944.0 38 993193.0 39 42905.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.09563048211819 23.280881493070936 10.43530561611909 24.188182408691787 2 16.62949246621469 21.981989714207977 37.78675268906046 23.601765130516878 3 16.358970583109066 26.360951817934126 30.928427269474255 26.351650329482553 4 12.465651902678037 16.667682413827475 41.10956192394476 29.757103759549725 5 12.918726893747237 36.79301147772948 37.16484225557333 13.123419372949947 6 29.447508927753546 40.13022554960096 18.217126374668116 12.20513914797738 7 26.908865519146048 31.97830568516114 25.259953421886316 15.852875373806496 8 24.11261786168527 35.74626786133 22.929933137696555 17.211181139288176 9 23.477641909410714 14.985244578152601 25.79564845999772 35.74146505243896 10-11 23.24258523826255 26.335482256742225 30.999968058941903 19.421964446053327 12-13 22.377629744477456 25.37463570414126 31.110824286632553 21.13691026474873 14-15 20.401017806846188 26.59273210806843 27.04477639384005 25.961473691245335 16-17 20.74194164489226 30.809481313648057 28.02904203989694 20.41953500156274 18-19 20.953372609216498 29.685174641602636 29.413608315721103 19.94784443345976 20-21 21.071856249260858 29.203741773840726 29.28776491366472 20.436637063233697 22-23 21.32098585506281 28.9627454346218 29.159174789018845 20.55709392129654 24-25 20.813136591475686 28.91911066476019 29.484686140385847 20.78306660337828 26-27 21.114594852501035 29.43448875458914 29.17426120408333 20.2766551888265 28-29 21.191316773466156 29.22415761606417 29.096693973083674 20.487831637386005 30-31 20.94935728320837 29.078203348619 29.32306569949223 20.649373668680397 32-33 21.077301190720494 29.343877627746295 29.270677670682172 20.308143510851043 34-35 20.973583496104318 29.108555324871173 29.298412006544744 20.61944917247977 36-37 20.967474111436516 29.216017476947105 29.38843307855008 20.4280753330663 38-39 21.02427930596744 29.220045363730286 29.394234909710075 20.361440420592196 40-41 20.886202699859655 29.13228815191793 29.532571151608117 20.4489379966143 42-43 20.843806486288965 29.154845381672146 29.562673062015847 20.438675070023045 44-45 20.82652875945451 29.017538719941683 29.559077791155786 20.596854729448015 46-47 20.83722599121577 29.05571982608734 29.673105333218157 20.43394884947873 48-49 20.81603709807449 29.20223512236343 29.563359268833832 20.418368510728254 50-51 20.62047575705342 29.13968034546296 29.70656140459449 20.53328249288913 52-53 20.734171260199204 28.987901361784473 29.840058822584446 20.437868555431876 54-55 20.702472920513383 29.20973236289995 29.67699895413059 20.410795762456075 56-57 20.74369768823215 29.1092231100282 29.58732298711787 20.559756214621782 58-59 20.759412369165624 29.11121052861437 29.569011287516023 20.56036581470398 60-61 20.561833919552406 29.113936587498205 29.774550634156533 20.54967885879286 62-63 20.536279530425123 29.141512367592792 29.651881157508182 20.670326944473903 64-65 20.709086861198504 28.984131984516438 29.747725625111116 20.559055529173946 66-67 20.57528897850124 28.963910791517062 29.741942742111092 20.718857487870604 68-69 20.60172755699787 29.14143565882031 29.60901317934408 20.647823604837747 70-71 20.75610846822987 28.98174331438068 29.57518204873445 20.686966168654997 72-73 20.680561620690238 29.055184324100846 29.51306907719141 20.751184978017506 74-75 20.60639087626899 29.116394824737807 29.631008356074386 20.646205942918822 76-77 20.642088163962786 29.117906471925192 29.543287386346307 20.69671797776572 78-79 20.851382014133367 28.987485725832602 29.480199800387584 20.680932459646446 80-81 20.74883668063788 29.130283053194944 29.46287400708414 20.65800625908304 82-83 20.847730499191766 29.087784696875207 29.458028602762187 20.60645620117084 84-85 20.78778274486806 29.103668387654697 29.483560092391308 20.624988775085935 86-87 20.655870224782383 29.053976485856246 29.584946532162288 20.705206757199086 88-89 20.764807818000435 28.965948540288274 29.587641112155854 20.681602529555434 90-91 20.97022811720748 28.996825400795274 29.47396656260336 20.558979919393888 92-93 21.09094498752945 28.930942790280135 29.358211474724367 20.61990074746605 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 91955.0 1 48635.5 2 5216.5 3 4915.5 4 4385.5 5 3662.0 6 3106.5 7 2826.0 8 2720.0 9 2672.5 10 2774.0 11 3084.5 12 3447.5 13 4071.5 14 4480.0 15 5034.5 16 6278.5 17 8144.0 18 10813.5 19 14581.5 20 19800.0 21 26314.0 22 34753.5 23 45438.5 24 58100.0 25 72598.0 26 89163.5 27 107466.5 28 126572.0 29 136288.0 30 145953.0 31 164365.0 32 181432.0 33 197375.0 34 210571.5 35 220996.5 36 228961.5 37 233001.5 38 233161.5 39 230587.0 40 226185.5 41 220476.0 42 213185.5 43 209011.0 44 204542.5 45 193158.5 46 180133.0 47 167706.5 48 155516.0 49 143980.0 50 132218.0 51 120787.5 52 109388.0 53 97807.5 54 88069.0 55 79344.0 56 70522.5 57 66199.0 58 62881.5 59 56674.0 60 51243.0 61 46026.0 62 41215.5 63 36837.0 64 31795.0 65 27579.0 66 24594.5 67 21577.5 68 18945.5 69 16985.5 70 15146.0 71 14085.0 72 13013.0 73 11121.5 74 9771.5 75 8697.5 76 7936.0 77 7613.0 78 6494.0 79 5738.5 80 5116.0 81 3751.5 82 2831.0 83 2195.0 84 1798.5 85 1387.0 86 1173.0 87 981.5 88 703.5 89 531.5 90 394.0 91 281.5 92 195.0 93 138.0 94 81.0 95 46.5 96 42.5 97 47.0 98 40.5 99 74.5 100 115.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.459512080514987 2 2.4094647788639123 3 2.2903591519484734 4 2.198518877911693 5 2.132101938012246 6 2.1124131905224313 7 2.1252204340352234 8 2.1735126046214512 9 2.126819167282411 10-11 2.070985146377931 12-13 1.8507509528102601 14-15 1.7112180331549474 16-17 1.6443145222672262 18-19 1.5833454396829225 20-21 1.8439650252773627 22-23 2.106730736480799 24-25 2.3055036739585124 26-27 2.500444864903565 28-29 2.5898783503019174 30-31 2.6949689950015254 32-33 2.7023631362697658 34-35 2.4430121107518983 36-37 2.255169642975062 38-39 2.1981105058322483 40-41 2.1207717849995724 42-43 2.035361199022131 44-45 1.949959301812338 46-47 1.927490148675241 48-49 2.257298391048762 50-51 2.562691196332089 52-53 3.1840249708231183 54-55 3.44792891058841 56-57 3.689320253378366 58-59 3.7081922567092924 60-61 3.4983324517130425 62-63 3.323036564419566 64-65 3.134681459351512 66-67 2.9198516792607454 68-69 2.7000779903784062 70-71 2.483953583908124 72-73 2.8434774116369708 74-75 2.692075635374823 76-77 3.3305697259701708 78-79 3.8549281647445954 80-81 4.29294631940327 82-83 4.118076181724533 84-85 3.7261693169724994 86-87 3.349598127118756 88-89 3.0094415624767574 90-91 2.7255187020510356 92-93 2.5639771339439568 >>END_MODULE >>Sequence Length Distribution pass #Length Count 93 5754556.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.22378492485096 #Duplication Level Percentage of deduplicated Percentage of total 1 93.98771846033658 80.09989103641169 2 3.618547013649288 6.167725448634173 3 0.9713905747438474 2.4835674425999104 4 0.54498422109758 1.8578247218503037 5 0.23692667063781853 1.0095893810699224 6 0.19275777329921298 0.9856528208547183 7 0.09506834767304007 0.5671459090673686 8 0.0651465748497549 0.4441630146868974 9 0.056338782427265574 0.4321263848858329 >10 0.21819813915428746 3.1505428608836263 >50 0.008831990572253584 0.5019686534683722 >100 0.003760204485718251 0.535107781806172 >500 2.1439143718019922E-4 0.14225588580557277 >1k 9.673511146995035E-5 0.10572754868572165 >5k 0.0 0.0 >10k+ 2.0120524724525475E-5 1.516711109289691 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 88451 1.5370603744233264 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.737753529551194E-5 0.0 0.0 2 3.475507059102388E-5 0.0 0.0 3 3.475507059102388E-5 0.0 1.737753529551194E-5 4 3.475507059102388E-5 0.0 3.475507059102388E-5 5 3.475507059102388E-5 0.0 3.475507059102388E-5 6 3.475507059102388E-5 0.0 5.2132605886535816E-5 7 3.475507059102388E-5 1.737753529551194E-5 5.2132605886535816E-5 8 3.475507059102388E-5 1.737753529551194E-5 5.2132605886535816E-5 9 3.475507059102388E-5 1.737753529551194E-5 6.951014118204776E-5 10-11 4.3443838238779844E-5 3.475507059102388E-5 9.557644412531566E-5 12-13 8.688767647755969E-5 3.475507059102388E-5 1.129539794208276E-4 14-15 1.2164274706858358E-4 3.475507059102388E-5 1.3033151471633956E-4 16-17 1.9115288825063132E-4 3.475507059102388E-5 1.6508658530736342E-4 18-19 2.0853042354614326E-4 3.475507059102388E-5 2.0853042354614326E-4 20-21 2.259079588416552E-4 3.475507059102388E-5 2.9541810002370295E-4 22-23 2.6066302943267907E-4 4.3443838238779844E-5 4.6919345297882233E-4 24-25 2.9541810002370295E-4 5.2132605886535816E-5 0.0011295397942082761 26-27 2.9541810002370295E-4 5.2132605886535816E-5 0.004874398650391099 28-29 3.127956353192149E-4 5.2132605886535816E-5 0.02353787155777092 30-31 3.3017317061472684E-4 5.2132605886535816E-5 0.040063907623802776 32-33 3.4755070591023876E-4 5.2132605886535816E-5 0.056563877386891365 34-35 3.649282412057507E-4 5.2132605886535816E-5 0.08496745882740563 36-37 3.8230577650126264E-4 5.2132605886535816E-5 0.1499768183679158 38-39 3.8230577650126264E-4 6.951014118204776E-5 0.26141895221803385 40-41 3.996833117967746E-4 6.951014118204776E-5 0.39042108548426674 42-43 4.518159176833104E-4 6.951014118204776E-5 0.5197360143858188 44-45 4.6919345297882233E-4 6.951014118204776E-5 0.6716069841009454 46-47 5.039485235698463E-4 6.951014118204776E-5 0.8502219806358648 48-49 5.213260588653581E-4 6.951014118204776E-5 1.0353187978360103 50-51 5.213260588653581E-4 8.688767647755969E-5 1.220936941095021 52-53 5.213260588653581E-4 8.688767647755969E-5 1.3952162425737105 54-55 5.734586647518939E-4 1.0426521177307163E-4 1.5798699326238201 56-57 5.734586647518939E-4 1.3033151471633956E-4 1.7830828303695367 58-59 5.734586647518939E-4 1.3902028236409552E-4 1.9948802305512363 60-61 6.777238765249656E-4 1.3902028236409552E-4 2.202654731312025 62-63 6.951014118204775E-4 1.5639781765960746E-4 2.400967164104407 64-65 7.037901794682335E-4 1.5639781765960746E-4 2.592710888555086 66-67 7.385452500592573E-4 1.5639781765960746E-4 2.802805290277825 68-69 7.906778559457932E-4 1.5639781765960746E-4 3.01903396195988 70-71 7.993666235935492E-4 1.8246412060287536E-4 3.22974526618561 72-73 7.993666235935492E-4 1.9115288825063132E-4 3.4325063480136433 74-75 7.993666235935492E-4 1.9984165589838728E-4 3.6222690334406336 76-77 8.167441588890611E-4 2.259079588416552E-4 3.8168974287503676 78-79 8.688767647755969E-4 2.4328549413716715E-4 4.0223694060845006 80-81 8.862543000711089E-4 2.4328549413716715E-4 4.228301888103965 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACTG 2465 0.0 15.240807 5 GTATTAG 2455 0.0 13.926314 1 GTATACG 705 0.0 13.504987 1 GCGCTTA 1135 0.0 13.345436 1 GTATATA 5085 0.0 13.276825 1 ACAGTGC 1465 0.0 13.252046 8 GTATAAG 2525 0.0 13.197447 1 GTATAGG 1960 0.0 13.027363 1 TACACCG 1135 0.0 12.935663 5 CTACACC 1845 0.0 12.874143 4 CTGCGCG 755 0.0 12.565934 9 TAAGCGC 1195 0.0 12.276348 6 GTCTGGG 2135 0.0 12.162251 1 CTACACT 2165 0.0 11.769177 4 ACCGTGC 955 0.0 11.745676 8 TATACAG 2290 0.0 11.691275 5 ACACCCG 1480 0.0 11.670888 5 ATTACGC 565 1.6745616E-8 11.471057 3 CACCCGG 1470 0.0 11.447364 6 GTATAAT 3675 0.0 11.422863 1 >>END_MODULE