##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename lane2_Undetermined_L002_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5754556 Sequences flagged as poor quality 0 Sequence length 93 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.783978294763315 33.0 33.0 33.0 27.0 33.0 2 31.909335142450608 33.0 33.0 33.0 27.0 33.0 3 31.998610839828476 33.0 33.0 33.0 27.0 33.0 4 35.94205825783953 37.0 37.0 37.0 33.0 37.0 5 35.4917819897834 37.0 37.0 37.0 33.0 37.0 6 35.36806888315971 37.0 37.0 37.0 33.0 37.0 7 35.229447589006 37.0 37.0 37.0 33.0 37.0 8 35.192655697502985 37.0 37.0 37.0 33.0 37.0 9 35.23774935894272 37.0 37.0 37.0 33.0 37.0 10-11 35.15965219558207 37.0 37.0 37.0 33.0 37.0 12-13 35.14331496643703 37.0 37.0 37.0 33.0 37.0 14-15 36.60403374300294 37.0 37.0 40.0 33.0 40.0 16-17 36.56537220247748 37.0 37.0 40.0 33.0 40.0 18-19 36.47151352771613 37.0 37.0 40.0 33.0 40.0 20-21 36.472841518963406 37.0 37.0 40.0 33.0 40.0 22-23 36.34701513027243 37.0 37.0 40.0 30.0 40.0 24-25 36.10901640369822 37.0 37.0 40.0 27.0 40.0 26-27 35.05052440188261 37.0 33.0 40.0 27.0 40.0 28-29 34.36368800651171 37.0 33.0 40.0 22.0 40.0 30-31 33.95908294228086 37.0 33.0 40.0 22.0 40.0 32-33 33.723500475101815 37.0 33.0 40.0 15.0 40.0 34-35 33.6137596888448 37.0 33.0 40.0 15.0 40.0 36-37 33.430713160146496 37.0 33.0 40.0 15.0 40.0 38-39 33.234898574277494 37.0 33.0 40.0 15.0 40.0 40-41 33.08757765151647 37.0 33.0 40.0 15.0 40.0 42-43 32.999592670572675 37.0 33.0 40.0 15.0 40.0 44-45 32.854625187416715 37.0 33.0 40.0 15.0 40.0 46-47 32.741763135157605 37.0 33.0 40.0 15.0 40.0 48-49 32.50172871721119 37.0 33.0 40.0 15.0 40.0 50-51 32.29017712226626 37.0 33.0 40.0 15.0 40.0 52-53 31.9995471414302 37.0 33.0 40.0 15.0 40.0 54-55 31.606133383705014 37.0 27.0 40.0 10.5 40.0 56-57 31.388536578669147 37.0 27.0 40.0 10.5 40.0 58-59 31.18733295844197 37.0 27.0 40.0 10.5 40.0 60-61 30.878007877584302 37.0 27.0 37.0 6.0 40.0 62-63 30.608493948099557 37.0 27.0 37.0 6.0 40.0 64-65 30.331072023627886 37.0 27.0 37.0 6.0 40.0 66-67 30.118694561318023 33.0 27.0 37.0 6.0 40.0 68-69 29.878710277560945 33.0 27.0 37.0 6.0 40.0 70-71 29.614075699324154 33.0 27.0 37.0 6.0 40.0 72-73 29.3364608842107 33.0 27.0 37.0 6.0 40.0 74-75 29.09569600156815 33.0 27.0 37.0 6.0 37.0 76-77 27.704646631295276 33.0 22.0 37.0 6.0 37.0 78-79 28.752829931622877 33.0 27.0 37.0 6.0 37.0 80-81 28.701287988161035 33.0 27.0 37.0 6.0 37.0 82-83 28.646874928317665 33.0 27.0 37.0 6.0 37.0 84-85 28.52489705548091 33.0 27.0 37.0 6.0 37.0 86-87 28.311116791634316 33.0 27.0 37.0 6.0 37.0 88-89 28.144794316016736 33.0 24.5 37.0 6.0 37.0 90-91 27.99727798287131 33.0 22.0 37.0 6.0 37.0 92-93 26.94379418672787 33.0 22.0 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2304 1 0.3961976498258615 2304 2 0.3880419781193609 2304 3 0.4443293333762419 2304 4 0.43023978716284006 2304 5 0.42571106605399933 2304 6 0.6459957943797932 2304 7 0.7476261594356473 2304 8 0.7575238423737929 2304 9 0.6038370016912893 2304 10-11 0.7483535814506297 2304 12-13 0.7260154042072529 2304 14-15 1.0498622799856037 2304 16-17 0.9804107199601546 2304 18-19 0.9025593373133347 2304 20-21 0.9456685191683576 2304 22-23 0.9104839607363644 2304 24-25 0.9318656273252373 2304 26-27 0.9101371935693834 2304 28-29 0.5346262879757262 2304 30-31 0.37060376341220547 2304 32-33 0.20103274587865627 2304 34-35 0.0920171412350399 2304 36-37 -0.12384027014670096 2304 38-39 0.1923818504823558 2304 40-41 0.009775470618627935 2304 42-43 0.17900729305016227 2304 44-45 0.018738650549991576 2304 46-47 -0.10207273292831331 2304 48-49 -0.18161056662918185 2304 50-51 0.13317738793049472 2304 52-53 0.011897119779685994 2304 54-55 -0.17120434580022348 2304 56-57 -0.12084543945393733 2304 58-59 -0.34058784456630775 2304 60-61 -0.38889031540148267 2304 62-63 -0.40604489290399925 2304 64-65 -0.25161349427361657 2304 66-67 -0.46509467883738154 2304 68-69 -0.5071240180827381 2304 70-71 -0.3780793074234161 2304 72-73 -0.22908453820171815 2304 74-75 -0.42039761133132814 2304 76-77 -0.3735467797595504 2304 78-79 -0.1864355979427934 2304 80-81 0.006711655978886455 2304 82-83 -0.13700546869369035 2304 84-85 -0.1007212360404317 2304 86-87 0.01933335930078428 2304 88-89 -0.1537505165726003 2304 90-91 -0.18579794315708753 2304 92-93 0.13341761504714 2305 1 0.33051793133904184 2305 2 0.3838721710322659 2305 3 0.5189668185851026 2305 4 0.5053861095216234 2305 5 0.5181110020538071 2305 6 0.5871558910266614 2305 7 0.6964436079265894 2305 8 0.6686178895065353 2305 9 0.7800597245542917 2305 10-11 0.732517335094812 2305 12-13 0.7319048055177717 2305 14-15 1.0251533356572793 2305 16-17 0.9958194992742833 2305 18-19 0.9948595469851966 2305 20-21 0.8749793335297156 2305 22-23 0.9686253003403991 2305 24-25 0.8519693591447037 2305 26-27 0.7314653537364393 2305 28-29 0.6339460393503202 2305 30-31 0.3467433490383556 2305 32-33 0.13795300098079366 2305 34-35 0.2808668542067778 2305 36-37 0.09086640431176818 2305 38-39 0.27777673112446877 2305 40-41 0.21753373372572327 2305 42-43 0.3688936187349725 2305 44-45 0.3831962215180056 2305 46-47 0.39043046365666356 2305 48-49 0.4115105016808869 2305 50-51 0.34948074528308126 2305 52-53 0.39217501239799546 2305 54-55 0.2285728521584538 2305 56-57 0.2453789861539235 2305 58-59 0.339002765158817 2305 60-61 0.31124521925355353 2305 62-63 0.30639263088031754 2305 64-65 0.5057220072854314 2305 66-67 0.5533969864799673 2305 68-69 0.4068960995121351 2305 70-71 0.28703420387757816 2305 72-73 0.5413309685299446 2305 74-75 0.4561242768168299 2305 76-77 0.5768611187300081 2305 78-79 0.847705292618798 2305 80-81 0.8927334700172302 2305 82-83 0.8175425792593316 2305 84-85 0.8434575683340491 2305 86-87 0.7297877949168914 2305 88-89 0.4317436451906289 2305 90-91 0.3254619195438657 2305 92-93 0.5889089192675101 2306 1 0.2182990358813477 2306 2 0.2297676270334037 2306 3 0.2544550819970759 2306 4 0.31739499520235626 2306 5 0.3143213371646212 2306 6 0.46103446355662214 2306 7 0.5288770203906026 2306 8 0.5526832748322832 2306 9 0.3781997638315744 2306 10-11 0.5448104812256247 2306 12-13 0.5292914936022584 2306 14-15 0.6411389531900653 2306 16-17 0.7988213479161033 2306 18-19 0.8056080690036964 2306 20-21 0.6946439007763985 2306 22-23 0.7913824369280889 2306 24-25 0.9790412358973981 2306 26-27 0.9641503915988636 2306 28-29 0.5574113870593465 2306 30-31 0.5724398769467456 2306 32-33 0.4032152420904538 2306 34-35 0.21917061096550583 2306 36-37 0.18734774929612286 2306 38-39 0.4071640698766359 2306 40-41 0.1817887276850172 2306 42-43 0.38153551597353896 2306 44-45 0.3426431878645957 2306 46-47 0.3367978909606464 2306 48-49 0.3128552149211359 2306 50-51 0.45632448167454953 2306 52-53 0.4417344730893582 2306 54-55 0.3955365215175952 2306 56-57 0.4946650986347123 2306 58-59 0.47277607863322757 2306 60-61 0.36346191192998134 2306 62-63 0.4388077772043033 2306 64-65 0.35481241112301376 2306 66-67 0.30876026747182905 2306 68-69 0.4704375278971753 2306 70-71 0.39815584315350705 2306 72-73 0.47431792298151265 2306 74-75 0.45563871604802486 2306 76-77 0.5590607822128071 2306 78-79 0.7269559742101528 2306 80-81 0.8372190782305537 2306 82-83 0.8386409198872613 2306 84-85 0.8231057333325325 2306 86-87 0.9284583994786821 2306 88-89 0.7597531273167526 2306 90-91 0.8939648924513541 2306 92-93 0.7828385285449428 2307 1 0.33614484740806105 2307 2 0.3196659550224794 2307 3 0.4666694698535174 2307 4 0.3465035958215381 2307 5 0.40313700822610343 2307 6 0.6151824544349722 2307 7 0.5182592704554025 2307 8 0.6445412427317692 2307 9 0.6353936629284362 2307 10-11 0.6836237058680581 2307 12-13 0.5499792868448949 2307 14-15 0.6884158266665139 2307 16-17 0.7867315526872005 2307 18-19 0.6995302029800854 2307 20-21 0.4699494847278771 2307 22-23 0.5427315787883558 2307 24-25 0.6128518830626035 2307 26-27 0.8816505881861048 2307 28-29 0.5911685042457293 2307 30-31 0.4135644142386212 2307 32-33 0.4495187320237264 2307 34-35 0.15329894311931014 2307 36-37 0.20281200099769592 2307 38-39 0.18444918545537803 2307 40-41 0.05121210088915262 2307 42-43 0.25208269836863906 2307 44-45 0.16834442185001564 2307 46-47 0.24208078728781146 2307 48-49 0.07724059581983767 2307 50-51 0.1617255050931874 2307 52-53 0.41933625431542865 2307 54-55 0.2628676821978182 2307 56-57 0.04303671551383559 2307 58-59 0.1312644828981533 2307 60-61 0.23011902591272815 2307 62-63 0.13985213602408209 2307 64-65 0.25150706598861916 2307 66-67 0.13886511400271928 2307 68-69 0.09123849714510612 2307 70-71 0.10523257625210292 2307 72-73 0.23154915909298524 2307 74-75 0.21574426155578763 2307 76-77 0.2642798095282437 2307 78-79 0.3420885031376031 2307 80-81 0.4193043592338199 2307 82-83 0.2252092638071801 2307 84-85 0.32314272085870854 2307 86-87 0.4049837854449727 2307 88-89 0.1951062708196183 2307 90-91 0.4860956943234491 2307 92-93 0.40132984521350323 2308 1 0.33422220484879617 2308 2 0.37467336463348744 2308 3 0.411730922798359 2308 4 0.3096345834207668 2308 5 0.379197629311264 2308 6 0.48748648537354455 2308 7 0.5373920429720798 2308 8 0.5416275302687552 2308 9 0.5959657179523745 2308 10-11 0.5174941671370021 2308 12-13 0.6058571191618185 2308 14-15 0.49549599920852927 2308 16-17 0.5730441556367225 2308 18-19 0.5116097970766305 2308 20-21 0.5288063112107722 2308 22-23 0.564048671576522 2308 24-25 0.6508185646035827 2308 26-27 0.7530621604008871 2308 28-29 0.42438339398300684 2308 30-31 0.4132738142529391 2308 32-33 0.25211319767783635 2308 34-35 0.10733731884443642 2308 36-37 0.1436576882735494 2308 38-39 0.24683590655100573 2308 40-41 0.199555818695913 2308 42-43 0.181527500898369 2308 44-45 0.10450194632377219 2308 46-47 0.27681781495954283 2308 48-49 0.2524310696493117 2308 50-51 0.2085313843027592 2308 52-53 0.1412967988254259 2308 54-55 0.27218616325510325 2308 56-57 0.35271851541577703 2308 58-59 0.4679913629836534 2308 60-61 0.24122380166869561 2308 62-63 0.14713228542828105 2308 64-65 0.14335153577448523 2308 66-67 0.23392436334577127 2308 68-69 0.09482623630476184 2308 70-71 0.24176774298212322 2308 72-73 0.45125789802677474 2308 74-75 0.2548843610484077 2308 76-77 0.3195227290802478 2308 78-79 0.36780591324442113 2308 80-81 0.6434836552932275 2308 82-83 0.383784580161759 2308 84-85 0.5092795865657038 2308 86-87 0.46676176154690907 2308 88-89 0.24227574432405774 2308 90-91 0.39565436797553133 2308 92-93 0.38268186508999236 2309 1 0.40872354956700363 2309 2 0.3638147952207049 2309 3 0.39572277006731227 2309 4 0.3501152568652941 2309 5 0.35300613661375024 2309 6 0.5704563950422425 2309 7 0.6787878484160785 2309 8 0.4791036569978928 2309 9 0.564359171647034 2309 10-11 0.6250136142949998 2309 12-13 0.5513958144836479 2309 14-15 0.6681528970702573 2309 16-17 0.7175784489662007 2309 18-19 0.8389074320610206 2309 20-21 0.764514790774669 2309 22-23 0.7178413799475436 2309 24-25 0.795046744590806 2309 26-27 0.9424000042688547 2309 28-29 0.979882724638955 2309 30-31 0.919269279597799 2309 32-33 0.858831699312006 2309 34-35 0.764604248424618 2309 36-37 0.5926898047273497 2309 38-39 0.7841707835967284 2309 40-41 0.8109338473269503 2309 42-43 0.8223673286779558 2309 44-45 0.8065543601834122 2309 46-47 0.5896414096385669 2309 48-49 0.7503769914747522 2309 50-51 0.7158541769749434 2309 52-53 0.9067749275565653 2309 54-55 1.0141552166458503 2309 56-57 1.0281076877206132 2309 58-59 0.9888173938337772 2309 60-61 1.162363616182006 2309 62-63 1.2935338918539827 2309 64-65 1.1936851947346518 2309 66-67 1.1955807184960996 2309 68-69 0.9541386812798578 2309 70-71 1.1312217753277203 2309 72-73 1.2635208839042171 2309 74-75 1.2716469790881355 2309 76-77 1.1105436536192457 2309 78-79 1.177257919467511 2309 80-81 1.5433447452988958 2309 82-83 1.4326364440738644 2309 84-85 1.2675553804318618 2309 86-87 1.107844868750501 2309 88-89 1.1548366184511991 2309 90-91 1.1908387089430015 2309 92-93 1.3550182496673386 2310 1 0.29691990874953333 2310 2 0.261546302545149 2310 3 0.32434982744351615 2310 4 0.2769870044902021 2310 5 0.4371223522880143 2310 6 0.4576110403633251 2310 7 0.5392297974836566 2310 8 0.5078006692296952 2310 9 0.579409329083397 2310 10-11 0.5486389320129632 2310 12-13 0.5340386642714705 2310 14-15 0.6754586812984371 2310 16-17 0.5250226688026416 2310 18-19 0.6820459923176827 2310 20-21 0.6248534008907001 2310 22-23 0.5202657373307957 2310 24-25 0.634544704150521 2310 26-27 0.6818044450039551 2310 28-29 0.6775831457338057 2310 30-31 0.8994668093914839 2310 32-33 0.6105884506492671 2310 34-35 0.46134244882674125 2310 36-37 0.6111292499877266 2310 38-39 0.6547170579641417 2310 40-41 0.5657096481929855 2310 42-43 0.6711936717645841 2310 44-45 0.5761333195317775 2310 46-47 0.6073430663785118 2310 48-49 0.7234956188247779 2310 50-51 0.6830662906326239 2310 52-53 0.7188576941009366 2310 54-55 0.8203007301160063 2310 56-57 0.9046764529968172 2310 58-59 0.9644300732868416 2310 60-61 0.9879978327125514 2310 62-63 1.0561863883448588 2310 64-65 1.0781365954034143 2310 66-67 1.0417395422486706 2310 68-69 0.9415951491774734 2310 70-71 1.087667886568294 2310 72-73 1.2358618352425594 2310 74-75 1.0692348047321083 2310 76-77 1.0996156516248696 2310 78-79 1.2807219313036917 2310 80-81 1.5576612980658204 2310 82-83 1.2396519817691711 2310 84-85 1.3787302296343817 2310 86-87 1.371672897300403 2310 88-89 1.223691749901075 2310 90-91 1.2197430494174277 2310 92-93 1.3062925791401838 2311 1 0.2524901004104798 2311 2 0.24313051761334492 2311 3 0.3176892249568475 2311 4 0.21831334844799954 2311 5 0.40335210388279563 2311 6 0.4972941698800213 2311 7 0.5602122701481917 2311 8 0.49039144613183083 2311 9 0.3660875056830619 2311 10-11 0.3967877445306698 2311 12-13 0.3290133265626949 2311 14-15 0.4599908802928496 2311 16-17 0.5439735368997489 2311 18-19 0.6486567596564967 2311 20-21 0.48916438927042805 2311 22-23 0.39897063184602644 2311 24-25 0.39954690693711825 2311 26-27 0.3350620864693994 2311 28-29 0.6091945410277049 2311 30-31 0.7194298231429599 2311 32-33 0.5152205550854774 2311 34-35 0.4768948628690808 2311 36-37 0.579854210660983 2311 38-39 0.5742123539459882 2311 40-41 0.5895167142175097 2311 42-43 0.47809984010278583 2311 44-45 0.38632087225761325 2311 46-47 0.431570529721526 2311 48-49 0.47495135820296497 2311 50-51 0.4361356031025281 2311 52-53 0.5349882942314714 2311 54-55 0.7905953447782856 2311 56-57 0.7143505675019526 2311 58-59 0.7351592515602547 2311 60-61 0.9034246341661003 2311 62-63 0.9699204796251877 2311 64-65 0.9917348484766109 2311 66-67 0.9232313492236557 2311 68-69 0.9044877500904676 2311 70-71 0.977068278097299 2311 72-73 0.7656627891230485 2311 74-75 0.801335361391903 2311 76-77 0.9028828265738049 2311 78-79 1.1277854044565352 2311 80-81 1.2027348853044195 2311 82-83 1.165415376852799 2311 84-85 1.0648643666639224 2311 86-87 0.9844891170870547 2311 88-89 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0.4828301580664487 1114 48-49 0.6837571518966641 1114 50-51 0.7769341485534369 1114 52-53 0.7135440332290877 1114 54-55 0.4451116154740298 1114 56-57 0.5821351745072718 1114 58-59 0.8975711564534237 1114 60-61 1.0677948593188589 1114 62-63 0.9526161356329794 1114 64-65 0.6642389657135581 1114 66-67 0.5670061633420467 1114 68-69 0.7757862166262548 1114 70-71 0.7042723374883622 1114 72-73 0.48415846792433825 1114 74-75 0.646849945867654 1114 76-77 0.7835566142812986 1114 78-79 0.7195110608417004 1114 80-81 0.6122217690962053 1114 82-83 0.583712319611994 1114 84-85 0.6384606813464409 1114 86-87 0.5881052826861541 1114 88-89 0.5715457273914346 1114 90-91 0.5472439965587519 1114 92-93 0.6672015455739597 1115 1 -0.6198842317399702 1115 2 -0.5813603587198308 1115 3 -1.4521691218245785 1115 4 -0.7023342838931157 1115 5 -0.45585028875519384 1115 6 -0.14037886278664757 1115 7 -0.09178321649694254 1115 8 -0.039677844911317095 1115 9 -1.1142870689932636 1115 10-11 -0.8894043930207545 1115 12-13 -0.29231256423873475 1115 14-15 -0.40840897154422606 1115 16-17 -0.3115492786765941 1115 18-19 -0.21092201214771933 1115 20-21 -0.25665538661527876 1115 22-23 -0.27993476700977027 1115 24-25 -0.3140037662509698 1115 26-27 0.3584075034948526 1115 28-29 0.4166676206932678 1115 30-31 0.8682682131941064 1115 32-33 1.2509723499329297 1115 34-35 1.3061572532750318 1115 36-37 1.27277681409565 1115 38-39 1.3957344754175267 1115 40-41 1.5517660550119388 1115 42-43 1.5945149051895555 1115 44-45 1.3950955657929214 1115 46-47 1.4370105114514473 1115 48-49 1.4276767655806353 1115 50-51 1.5430925583346635 1115 52-53 1.7769024373319304 1115 54-55 1.6392490028376336 1115 56-57 1.390123213641104 1115 58-59 1.3084069545442532 1115 60-61 1.6091718584505799 1115 62-63 1.6539827675434182 1115 64-65 1.5205518942725433 1115 66-67 1.614636435222586 1115 68-69 1.7775890132410055 1115 70-71 1.5485872995282044 1115 72-73 1.5328615482851866 1115 74-75 1.4081946748534797 1115 76-77 1.390455543447132 1115 78-79 1.54174250248073 1115 80-81 1.4971503846251117 1115 82-83 1.2593309366147736 1115 84-85 1.437943092709265 1115 86-87 1.415194156094696 1115 88-89 1.5909771544050564 1115 90-91 1.4638025085508417 1115 92-93 1.3389599070650888 1116 1 -1.3994427283126392 1116 2 -0.9116812066020756 1116 3 -0.9097497454027916 1116 4 -0.6558941535889176 1116 5 -0.24064755722461229 1116 6 -0.04925148304860727 1116 7 -1.5591492585021598 1116 8 -1.0189763644125236 1116 9 -1.201655562476894 1116 10-11 -1.698608434088186 1116 12-13 -0.6979982057911513 1116 14-15 -1.8595742214573505 1116 16-17 -1.653070481779082 1116 18-19 -0.6442548856159505 1116 20-21 -0.37056869897762823 1116 22-23 -0.14975345696082343 1116 24-25 -0.763504233525012 1116 26-27 -0.7213362136833439 1116 28-29 -0.811549364214244 1116 30-31 -0.2793000854316148 1116 32-33 -0.4914783682826851 1116 34-35 -0.23249361378925926 1116 36-37 -0.31092786030821884 1116 38-39 -0.022980322547553556 1116 40-41 0.44375291127246896 1116 42-43 0.36979374666560005 1116 44-45 0.45686397931679323 1116 46-47 0.3573296566973383 1116 48-49 0.18264198870803483 1116 50-51 0.30313577089323474 1116 52-53 0.8556568484176132 1116 54-55 0.8859801142049868 1116 56-57 0.9405682864164504 1116 58-59 0.8612844156528681 1116 60-61 1.1278566885397936 1116 62-63 1.0912713466914 1116 64-65 0.8498725213627623 1116 66-67 0.8517719076780566 1116 68-69 1.036346051235121 1116 70-71 0.9591696670170826 1116 72-73 0.754689161776632 1116 74-75 1.0123062606097228 1116 76-77 1.0969813301556783 1116 78-79 0.9769060364725135 1116 80-81 0.8491353355680111 1116 82-83 0.5624652692860721 1116 84-85 0.6438628368244217 1116 86-87 0.7330991571617531 1116 88-89 0.7009262166804966 1116 90-91 0.8124823919676594 1116 92-93 0.5074511594940674 2301 1 -0.33475607730889934 2301 2 -0.1580239353614381 2301 3 0.01917701288838458 2301 4 -0.08200335981127438 2301 5 -0.04412277620116356 2301 6 -0.026886819428817432 2301 7 -0.05061698692368566 2301 8 -0.04759259213155076 2301 9 -0.33030259388927163 2301 10-11 -1.4117686340761395 2301 12-13 -0.7657041432992315 2301 14-15 -1.0146860082961666 2301 16-17 -0.8564214488522239 2301 18-19 -0.7528311014182378 2301 20-21 -1.052087134003557 2301 22-23 -0.7314324048311676 2301 24-25 -0.739982304555852 2301 26-27 -0.5735154108975777 2301 28-29 -0.8616717475528617 2301 30-31 -0.7903885895936469 2301 32-33 -0.8726916074989362 2301 34-35 -0.8819555183862633 2301 36-37 -0.4895536079221898 2301 38-39 -0.5910952407601329 2301 40-41 -0.40650250296224755 2301 42-43 -0.3125811260945923 2301 44-45 -0.3307007199887906 2301 46-47 -0.9709514156013626 2301 48-49 -1.0453527651448624 2301 50-51 -0.9898054024134133 2301 52-53 -0.702614214844214 2301 54-55 -0.7117199228454503 2301 56-57 -0.662632467218927 2301 58-59 -0.777235068338868 2301 60-61 -0.645039976844906 2301 62-63 -0.6573263210601539 2301 64-65 -0.9243979219109946 2301 66-67 -1.0188399522990572 2301 68-69 -0.9994277515344123 2301 70-71 -1.1077445235749046 2301 72-73 -1.239061244538025 2301 74-75 -1.0864096779071346 2301 76-77 -1.1583309023614703 2301 78-79 -1.1815001226259199 2301 80-81 -1.3319255706841844 2301 82-83 -1.267409268736749 2301 84-85 -1.3979138185452484 2301 86-87 -1.538200214836717 2301 88-89 -1.3614604287701333 2301 90-91 -1.51493562573393 2301 92-93 -1.6021791678277566 2302 1 -0.3753757802670208 2302 2 -0.35261802211483584 2302 3 -0.1694655499858264 2302 4 -0.1881831361642341 2302 5 -0.106880316969729 2302 6 -0.180532501744743 2302 7 -0.35710694887345795 2302 8 -0.6475953208360394 2302 9 -0.3202833806330503 2302 10-11 -0.395823549962401 2302 12-13 -0.4549680120740476 2302 14-15 -0.6555120758158068 2302 16-17 -1.1421994867736132 2302 18-19 -0.6409189145566501 2302 20-21 -0.6650835752495539 2302 22-23 -1.0344596887793926 2302 24-25 -1.1709707685005597 2302 26-27 -0.7557296522051189 2302 28-29 -0.5057205830445142 2302 30-31 -0.30929596923895275 2302 32-33 -0.020089442111945743 2302 34-35 0.19900184712588498 2302 36-37 0.2012017755243818 2302 38-39 0.24160487049170598 2302 40-41 0.27385444243682144 2302 42-43 0.3522403665257343 2302 44-45 0.48467708840146884 2302 46-47 0.6040358918662818 2302 48-49 0.524390556205212 2302 50-51 0.5671829680377485 2302 52-53 0.28962376927757916 2302 54-55 -1.575792096168982E-4 2302 56-57 0.18468599843053823 2302 58-59 0.057001538929835505 2302 60-61 0.17721870326633393 2302 62-63 -0.23452077680342143 2302 64-65 -0.13181597704698333 2302 66-67 0.13603769781376585 2302 68-69 0.18686314342432553 2302 70-71 0.13418546716932767 2302 72-73 -0.010608722904841272 2302 74-75 0.231896694290878 2302 76-77 0.09420587010241022 2302 78-79 0.18699576941117257 2302 80-81 0.11485675024055553 2302 82-83 0.1592782575883085 2302 84-85 -0.11192589434239508 2302 86-87 -0.12235101589031316 2302 88-89 -0.03743061605101872 2302 90-91 0.06941698861235679 2302 92-93 -0.01815938001241335 2303 1 -0.8074791007802844 2303 2 -0.6588425327917697 2303 3 -0.6281269349972014 2303 4 -0.632333556993764 2303 5 -0.4605980099348628 2303 6 -0.43780352049102333 2303 7 -0.4448255824922285 2303 8 -0.6042161880300938 2303 9 -0.33692574650400786 2303 10-11 -0.6096616076337682 2303 12-13 -0.5989124541810611 2303 14-15 -0.9595277116486685 2303 16-17 -0.7526435379798571 2303 18-19 -0.7473805576075137 2303 20-21 -0.8116954336555295 2303 22-23 -0.9348382282012864 2303 24-25 -0.8917451950026845 2303 26-27 -0.7885827998869956 2303 28-29 -0.9045434484131292 2303 30-31 -0.7328025752962617 2303 32-33 -0.21831555900960353 2303 34-35 -0.01878257662686167 2303 36-37 -0.06683903147001757 2303 38-39 -0.1670899696231274 2303 40-41 -0.19385051818363053 2303 42-43 -0.18528700925877217 2303 44-45 -0.24456476583024767 2303 46-47 0.1869298995921369 2303 48-49 -0.012543114166831515 2303 50-51 0.15101982949789772 2303 52-53 -0.007062026056537718 2303 54-55 -0.6418548239586848 2303 56-57 -0.7491215708104093 2303 58-59 -1.1476550846714133 2303 60-61 -1.0413114428632646 2303 62-63 -1.1546938309182941 2303 64-65 -1.1908902959519025 2303 66-67 -1.3233004463536382 2303 68-69 -1.0116101756269842 2303 70-71 -0.9431791042431996 2303 72-73 -1.018857796972096 2303 74-75 -0.9776222906120431 2303 76-77 -0.8765006623282154 2303 78-79 -0.9995311347610603 2303 80-81 -1.0401074076719823 2303 82-83 -0.9296057055229987 2303 84-85 -0.7816064908669027 2303 86-87 -0.7733082184736162 2303 88-89 -0.9158583705541652 2303 90-91 -0.8573227435124657 2303 92-93 -0.9856394855538895 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 10.0 7 54.0 8 360.0 9 3115.0 10 18008.0 11 103284.0 12 79494.0 13 37710.0 14 19311.0 15 9804.0 16 12957.0 17 17964.0 18 25824.0 19 36848.0 20 50544.0 21 68251.0 22 93925.0 23 102585.0 24 122117.0 25 142263.0 26 163793.0 27 184513.0 28 203851.0 29 221461.0 30 236046.0 31 247014.0 32 257853.0 33 271194.0 34 294019.0 35 352046.0 36 508350.0 37 892614.0 38 956788.0 39 20586.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.35593315018204 24.759864075679644 10.800787201382144 26.08341557275618 2 15.341861300854486 22.842613748132784 36.68658363912003 25.1289413118927 3 14.656308451128405 27.07999776861473 30.66691483007497 27.59677895018189 4 11.237808790113434 17.46909752898399 40.088097152934125 31.204996527968447 5 11.688495168002536 37.68921529306518 37.321888951988655 13.300400586943631 6 28.406744151938046 41.66462191001356 17.778574055061764 12.150059882986628 7 25.979363134184464 32.5332658158162 25.25430285151452 16.23306819848482 8 23.19299699229619 36.65467848431747 22.61955570507959 17.532768818306746 9 22.4441294862714 14.778742269603423 25.475762161320525 37.30136608280465 10-11 22.538385237714255 26.96099403672499 30.716948449193993 19.783672276366758 12-13 21.457102859021617 25.829290739372418 31.075003875190372 21.63860252641559 14-15 19.479747872815906 27.18278351970161 26.655262369503397 26.682206237979088 16-17 19.818686985985895 31.68251028951398 27.64784086814486 20.850961856355266 18-19 20.044147259951988 30.37156133024663 29.183488580678542 20.400802829122835 20-21 20.196895897185392 30.023783072520644 28.979116043355884 20.800204986938077 22-23 20.29089992547156 29.772134185956062 28.86180080699307 21.075165081579307 24-25 20.041090310372862 29.576349545482945 29.109772771142122 21.272787373002075 26-27 20.38509957123118 30.188699235053633 28.759980270936314 20.666220922778876 28-29 20.383534617221475 30.099171592501843 28.68438662950712 20.83290716076956 30-31 20.289228267466804 29.721132562623115 28.927473917075417 21.06216525283466 32-33 20.490707004229435 30.04575331022575 28.75724416797909 20.706295517565728 34-35 20.22276437079761 29.77296093401544 28.907226017504783 21.09704867768216 36-37 20.333984344022415 29.863114565666855 28.925238049141004 20.877663041169725 38-39 20.268811582751052 29.902273455104616 28.957898631784612 20.871016330359716 40-41 20.255766462394245 29.784416484771736 29.073191070642384 20.88662598219163 42-43 20.254220965056117 29.86635982261451 29.00911197515984 20.870307237169534 44-45 20.13401320692735 29.642757224990472 29.06626003482896 21.156969533253218 46-47 20.075719420327346 29.645609969414856 29.25865651891037 21.020014091347427 48-49 20.111364833335955 29.930578580920695 29.0557990599984 20.902257525744947 50-51 20.088366455832315 29.868031136318564 29.092659070618815 20.950943337230314 52-53 20.069628135239732 29.71375588408389 29.2264523537343 20.990163626942074 54-55 20.029101199351523 29.7867065758281 29.18902904166788 20.995163183152503 56-57 19.971267615561768 29.804972782104322 29.08960634455925 21.13415325777466 58-59 19.992312964530544 29.737918120644107 29.0532011899715 21.216567724853842 60-61 19.887000935952027 29.747390783369056 29.16329063419449 21.20231764648443 62-63 19.93928517806995 29.865518408574527 29.01120743142316 21.183988981932362 64-65 19.923469080526534 29.717462779070125 29.05174290576627 21.307325234637073 66-67 19.866739464998854 29.566290956114464 29.07087875868176 21.496090820204923 68-69 19.892744526805675 29.79506424487679 28.919382777412768 21.392808450904766 70-71 20.010555749660153 29.746928773328523 28.865232828566693 21.377282648444627 72-73 19.983875634713776 29.747145439420013 28.782094018437142 21.486884907429065 74-75 19.889320686103222 29.84939405376715 28.85898800182382 21.40229725830581 76-77 19.843916576560865 29.757868545680356 28.936170291539238 21.46204458621954 78-79 20.0210153821666 29.74913753100558 28.760522694156855 21.469324392670966 80-81 19.930044192507822 29.78855946535297 28.69376968878582 21.587626653353393 82-83 19.846445722416554 29.7859449048604 28.776785283878436 21.590824088844613 84-85 19.822664877368577 29.841915587868616 28.794140368810083 21.54127916595272 86-87 19.71552863712388 29.674534821972138 28.89264678126768 21.717289759636305 88-89 19.79195998134611 29.704675075698283 28.81977009302556 21.68359484993005 90-91 19.905493152838158 29.72044414860448 28.78120556097136 21.592857137585998 92-93 19.955481595358133 29.643266752289833 28.62892568438354 21.7723259679685 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 427.0 1 308.0 2 326.5 3 814.5 4 1963.0 5 4236.0 6 8109.0 7 13415.0 8 19428.5 9 25065.5 10 28942.0 11 30733.0 12 30775.0 13 28929.0 14 27487.0 15 25798.5 16 23043.0 17 21447.5 18 21194.5 19 22554.5 20 25880.0 21 31353.5 22 38959.5 23 49140.0 24 61014.0 25 74538.0 26 90553.0 27 108757.5 28 127708.0 29 137012.0 30 146178.5 31 164047.0 32 179788.0 33 194595.0 34 207539.5 35 217425.0 36 224438.0 37 227574.5 38 226956.5 39 224121.5 40 219542.5 41 213294.5 42 205899.0 43 201557.0 44 197084.5 45 186140.0 46 174600.5 47 162766.5 48 150373.5 49 138703.0 50 127005.0 51 115805.5 52 105041.0 53 93969.0 54 83174.0 55 73803.5 56 65477.0 57 61579.0 58 58715.0 59 53121.0 60 47514.5 61 43009.5 62 38814.5 63 34149.0 64 29878.0 65 26428.5 66 24295.5 67 21990.5 68 18596.0 69 15499.0 70 13579.5 71 12812.0 72 12316.5 73 10803.0 74 9209.0 75 8481.0 76 8582.5 77 8130.0 78 6630.5 79 5191.0 80 3906.0 81 3368.5 82 2962.5 83 2451.0 84 2125.5 85 1423.0 86 1042.0 87 882.5 88 634.5 89 396.5 90 194.5 91 123.0 92 81.0 93 40.0 94 18.5 95 22.5 96 31.5 97 38.0 98 51.5 99 179.5 100 299.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.14115771920544348 2 0.0 3 0.004917842488629879 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 5.64769897104138E-4 18-19 0.007037901794682335 20-21 7.472340177070133E-4 22-23 0.029863294405337266 24-25 0.42988546814037437 26-27 2.4063715775813113 28-29 3.412278896929668 30-31 4.271876057857462 32-33 4.495038366122425 34-35 4.467555794052573 36-37 4.678710225428339 38-39 4.686903733320173 40-41 4.5992253789866675 42-43 4.592074523212564 44-45 4.438161693100215 46-47 4.340204526639415 48-49 4.429264395028913 50-51 4.40890661243022 52-53 5.12854510408796 54-55 5.860634599784936 56-57 5.825566733558593 58-59 5.618739308471409 60-61 5.857576153572926 62-63 6.075455691108054 64-65 6.210574716798307 66-67 6.060493633218619 68-69 6.145035342431284 70-71 6.195960209614782 72-73 6.281553259712826 74-75 6.167322031447778 76-77 6.126849751744531 78-79 5.890967087643252 80-81 6.159163278626536 82-83 5.912689006762642 84-85 5.884937082895709 86-87 6.132314986594969 88-89 5.947982780947826 90-91 5.807711316042454 92-93 5.552131215683712 >>END_MODULE >>Sequence Length Distribution pass #Length Count 93 5754556.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.93109738748295 #Duplication Level Percentage of deduplicated Percentage of total 1 93.4901944879153 79.40224812827857 2 3.8963128268860725 6.618362483047202 3 1.1454792947603292 2.9186044061590444 4 0.45189864402323365 1.5352099097923502 5 0.2852993975765897 1.2115395460083778 6 0.20848265830586662 1.0623996573706136 7 0.10519068669621955 0.625377231923698 8 0.08105335759940557 0.5507160486286077 9 0.045880354656097806 0.35070019825223697 >10 0.26916806251887243 3.8257368442229294 >50 0.01569829092599158 0.8737386704994495 >100 0.0049777296468745845 0.7302906197772431 >500 2.962332879202597E-4 0.17990509009296424 >1k 6.79752011225054E-5 0.11517116594666904 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 1.737753529551194E-5 0.0 2 0.0 1.737753529551194E-5 1.737753529551194E-5 3 0.0 1.737753529551194E-5 1.737753529551194E-5 4 0.0 1.737753529551194E-5 1.737753529551194E-5 5 1.737753529551194E-5 1.737753529551194E-5 1.737753529551194E-5 6 1.737753529551194E-5 1.737753529551194E-5 1.737753529551194E-5 7 1.737753529551194E-5 1.737753529551194E-5 1.737753529551194E-5 8 1.737753529551194E-5 1.737753529551194E-5 1.737753529551194E-5 9 3.475507059102388E-5 1.737753529551194E-5 5.2132605886535816E-5 10-11 5.2132605886535816E-5 1.737753529551194E-5 7.819890882980373E-5 12-13 8.688767647755969E-5 1.737753529551194E-5 1.0426521177307163E-4 14-15 8.688767647755969E-5 1.737753529551194E-5 1.2164274706858358E-4 16-17 1.0426521177307163E-4 1.737753529551194E-5 1.5639781765960746E-4 18-19 1.4770905001185148E-4 1.737753529551194E-5 1.9115288825063132E-4 20-21 1.9984165589838728E-4 1.737753529551194E-5 2.8672933237594697E-4 22-23 2.259079588416552E-4 1.737753529551194E-5 4.6050468533106635E-4 24-25 2.4328549413716715E-4 3.475507059102388E-5 0.001155606097151544 26-27 2.6066302943267907E-4 4.3443838238779844E-5 0.005135061679823778 28-29 2.7804056472819104E-4 5.2132605886535816E-5 0.02506709466377597 30-31 2.9541810002370295E-4 5.2132605886535816E-5 0.0422882321416283 32-33 2.9541810002370295E-4 6.951014118204776E-5 0.059127063842979374 34-35 2.9541810002370295E-4 6.951014118204776E-5 0.08865149631005415 36-37 3.127956353192149E-4 6.951014118204776E-5 0.1554420532183543 38-39 3.127956353192149E-4 6.951014118204776E-5 0.2699513220481302 40-41 3.214844029669709E-4 8.688767647755969E-5 0.40169041712340625 42-43 3.3886193826248277E-4 8.688767647755969E-5 0.5339682157928431 44-45 3.4755070591023876E-4 8.688767647755969E-5 0.6881330201669773 46-47 3.736170088535067E-4 8.688767647755969E-5 0.8687029894226419 48-49 3.8230577650126264E-4 8.688767647755969E-5 1.0544601529640167 50-51 3.9099454414901863E-4 8.688767647755969E-5 1.2398610770318337 52-53 4.1706084709228653E-4 8.688767647755969E-5 1.4135060984722365 54-55 4.518159176833104E-4 9.557644412531566E-5 1.5965089226692728 56-57 4.518159176833104E-4 1.0426521177307163E-4 1.7958987626499767 58-59 4.6050468533106635E-4 1.0426521177307163E-4 2.0027783203430465 60-61 5.8214743239965E-4 1.0426521177307163E-4 2.2059477742505242 62-63 6.255912706384298E-4 1.2164274706858358E-4 2.3979869168012264 64-65 6.429688059339417E-4 1.2164274706858358E-4 2.5834399734749303 66-67 6.951014118204775E-4 1.2164274706858358E-4 2.7873479726324675 68-69 7.298564824115015E-4 1.2164274706858358E-4 2.998676179361188 70-71 7.298564824115015E-4 1.2164274706858358E-4 3.204478329865936 72-73 7.298564824115015E-4 1.2164274706858358E-4 3.4035553742113205 74-75 7.298564824115015E-4 1.2164274706858358E-4 3.591032913746951 76-77 7.472340177070134E-4 1.2164274706858358E-4 3.78304598999471 78-79 7.646115530025253E-4 1.3902028236409552E-4 3.985416077278595 80-81 7.819890882980373E-4 1.5639781765960746E-4 4.186856466424169 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCCCG 1175 0.0 16.985981 5 TATACTG 2505 0.0 15.768964 5 TACACTG 1805 0.0 15.203865 5 GTATAGG 1840 0.0 13.803472 1 TAGACGG 550 7.2759576E-12 13.608088 5 TATACAG 2525 0.0 13.33862 5 GGCCTAC 1610 0.0 13.189267 1 GTATAAG 2420 0.0 13.075989 1 GCCTTAT 2205 0.0 13.029173 1 ATAGGAC 1880 0.0 12.828623 3 GTATTAG 2310 0.0 12.797429 1 TAGGACC 1615 0.0 12.6156845 4 GTCCTAG 2005 0.0 12.459862 1 CTACCCC 1875 0.0 12.418639 4 TAGTACT 2165 0.0 12.09957 4 GTCTTAG 2040 0.0 12.041988 1 GTGTAGG 1720 0.0 11.86162 1 CTATACT 2775 0.0 11.837266 4 ATAATAC 3980 0.0 11.806068 3 CTTACAC 1730 0.0 11.777684 3 >>END_MODULE