FastQCFastQC Report
Wed 5 Nov 2014
lane2_Undetermined_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane2_Undetermined_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5754556
Sequences flagged as poor quality0
Sequence length93
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCCG11750.016.9859815
TATACTG25050.015.7689645
TACACTG18050.015.2038655
GTATAGG18400.013.8034721
TAGACGG5507.2759576E-1213.6080885
TATACAG25250.013.338625
GGCCTAC16100.013.1892671
GTATAAG24200.013.0759891
GCCTTAT22050.013.0291731
ATAGGAC18800.012.8286233
GTATTAG23100.012.7974291
TAGGACC16150.012.61568454
GTCCTAG20050.012.4598621
CTACCCC18750.012.4186394
TAGTACT21650.012.099574
GTCTTAG20400.012.0419881
GTGTAGG17200.011.861621
CTATACT27750.011.8372664
ATAATAC39800.011.8060683
CTTACAC17300.011.7776843
Produced by FastQC (version 0.11.2)