##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename lane1_Undetermined_L001_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4964723 Sequences flagged as poor quality 0 Sequence length 93 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.109875415003014 33.0 27.0 33.0 15.0 33.0 2 29.076127308613188 33.0 27.0 33.0 15.0 33.0 3 29.11381783032004 33.0 27.0 33.0 15.0 33.0 4 32.54283451463455 37.0 33.0 37.0 15.0 37.0 5 32.52478980196881 37.0 33.0 37.0 15.0 37.0 6 32.589486059947355 37.0 33.0 37.0 15.0 37.0 7 32.51106879477465 37.0 33.0 37.0 15.0 37.0 8 32.46080576902276 37.0 33.0 37.0 15.0 37.0 9 32.54674047273131 37.0 33.0 37.0 15.0 37.0 10-11 32.523499196229075 37.0 33.0 37.0 15.0 37.0 12-13 32.505270384672016 37.0 33.0 37.0 15.0 37.0 14-15 33.998766597854505 37.0 33.0 40.0 15.0 40.0 16-17 33.94114545766199 37.0 33.0 40.0 15.0 40.0 18-19 33.98228541652777 37.0 33.0 40.0 15.0 40.0 20-21 33.80560506598253 37.0 33.0 40.0 15.0 40.0 22-23 33.63766588790553 37.0 33.0 40.0 15.0 40.0 24-25 33.461133481163 37.0 33.0 40.0 15.0 40.0 26-27 33.26264546884086 37.0 33.0 40.0 15.0 40.0 28-29 33.09265481679441 37.0 33.0 40.0 15.0 40.0 30-31 32.90932676405109 37.0 33.0 40.0 15.0 40.0 32-33 32.77318321686829 37.0 33.0 40.0 15.0 40.0 34-35 32.62226482726227 37.0 33.0 40.0 15.0 40.0 36-37 32.57737239318286 37.0 33.0 40.0 15.0 40.0 38-39 32.51063684721181 37.0 33.0 40.0 15.0 40.0 40-41 32.49210832104833 37.0 33.0 40.0 15.0 40.0 42-43 32.501705533219074 37.0 33.0 40.0 15.0 40.0 44-45 32.42082851349411 37.0 33.0 40.0 15.0 40.0 46-47 32.27103244229336 37.0 27.0 40.0 15.0 40.0 48-49 32.00821576551199 37.0 27.0 40.0 15.0 40.0 50-51 30.824274486210008 35.0 27.0 38.5 15.0 38.5 52-53 30.661002537301677 37.0 27.0 37.0 6.0 40.0 54-55 31.154422814727027 37.0 27.0 40.0 6.0 40.0 56-57 31.086890044016556 37.0 27.0 40.0 6.0 40.0 58-59 30.90590391447821 37.0 27.0 40.0 6.0 40.0 60-61 31.174452935239287 37.0 27.0 40.0 6.0 40.0 62-63 31.206645768555465 37.0 27.0 40.0 6.0 40.0 64-65 31.12322580736126 37.0 27.0 40.0 6.0 40.0 66-67 30.993017938765163 37.0 27.0 38.5 15.0 40.0 68-69 30.87337571501975 37.0 27.0 37.0 15.0 40.0 70-71 30.743954194423335 37.0 27.0 37.0 15.0 40.0 72-73 30.449347224407084 37.0 27.0 37.0 6.0 40.0 74-75 30.24936859518648 37.0 27.0 37.0 6.0 40.0 76-77 29.84662860344877 33.0 27.0 37.0 6.0 38.5 78-79 29.405630686747276 33.0 27.0 37.0 6.0 37.0 80-81 28.97534958143687 33.0 27.0 37.0 6.0 37.0 82-83 28.76352618262892 33.0 22.0 37.0 6.0 37.0 84-85 28.676999099446235 33.0 22.0 37.0 6.0 37.0 86-87 28.60044578519285 33.0 22.0 37.0 6.0 37.0 88-89 28.567027002312113 33.0 22.0 37.0 6.0 37.0 90-91 28.497044749525806 33.0 22.0 37.0 6.0 37.0 92-93 27.71849416372273 33.0 22.0 37.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2304 1 -0.4244153274834339 2304 2 -0.5430876506612101 2304 3 -0.5177165413837663 2304 4 -0.7215930902930872 2304 5 -0.5093827486915572 2304 6 -0.45429521094929015 2304 7 -0.530452018838588 2304 8 -0.3988845696720844 2304 9 -0.4459169416664963 2304 10-11 -0.4856779428053386 2304 12-13 -0.4041103377383095 2304 14-15 -0.394041727772823 2304 16-17 -0.2961363866222442 2304 18-19 -0.2324640177398436 2304 20-21 -0.32999476309463205 2304 22-23 -0.3482622393424819 2304 24-25 -0.3357555104612189 2304 26-27 -0.3534038666063637 2304 28-29 -0.4896253804404722 2304 30-31 -0.6045917900867437 2304 32-33 -0.61238279843257 2304 34-35 -0.5258241176138441 2304 36-37 -0.43894634770477836 2304 38-39 -0.24884355167318972 2304 40-41 -0.3436556174528391 2304 42-43 -0.2676617187013193 2304 44-45 -0.10816712016005425 2304 46-47 -0.2919148084038028 2304 48-49 -0.28503079762223393 2304 50-51 -0.48512475158084456 2304 52-53 -0.43790269309818086 2304 54-55 -0.4779552193441994 2304 56-57 -0.5340731803570904 2304 58-59 -0.7534617475814578 2304 60-61 -0.6871127651837519 2304 62-63 -0.6243434383553073 2304 64-65 -0.5496611922624126 2304 66-67 -0.3864916066959161 2304 68-69 -0.21229242379757096 2304 70-71 -0.3109521069008423 2304 72-73 -0.29953150083644786 2304 74-75 -0.2184045813011899 2304 76-77 -0.47807494501382664 2304 78-79 -0.6134072818555332 2304 80-81 -0.7375109387718553 2304 82-83 -0.6301646644912999 2304 84-85 -0.6466818238493062 2304 86-87 -0.5615969336635196 2304 88-89 -0.37990400377170275 2304 90-91 -0.49509155481696965 2304 92-93 -0.46030220263477517 2305 1 0.10865278228371977 2305 2 0.2356931698428042 2305 3 0.327299428295067 2305 4 0.28236935987604284 2305 5 0.2367923100492817 2305 6 0.2668822360320462 2305 7 0.2654538295373712 2305 8 0.3939891181254964 2305 9 0.5472755584138369 2305 10-11 0.5177354588267349 2305 12-13 0.6437816438169008 2305 14-15 0.9429449280225484 2305 16-17 1.0893091767946643 2305 18-19 0.9832305116594284 2305 20-21 0.7895526590259649 2305 22-23 0.8104320363713455 2305 24-25 0.6495640766972741 2305 26-27 0.8497111338361876 2305 28-29 0.7303032169269699 2305 30-31 0.6486248216257167 2305 32-33 0.6464689640560053 2305 34-35 0.7359788081809171 2305 36-37 0.6456201786945712 2305 38-39 0.6942020400845408 2305 40-41 0.6863801672187932 2305 42-43 0.7625727478370479 2305 44-45 0.9375256510993708 2305 46-47 0.7842645793313991 2305 48-49 0.48371771281215814 2305 50-51 0.6188177810464701 2305 52-53 0.4789631764061646 2305 54-55 0.5941346561503238 2305 56-57 0.6239777784981619 2305 58-59 0.5504010271361182 2305 60-61 0.5950905602341123 2305 62-63 0.6717495208485005 2305 64-65 0.5421618013331546 2305 66-67 0.589320096197973 2305 68-69 0.506136928868063 2305 70-71 0.5446386406990449 2305 72-73 0.5285230258471891 2305 74-75 0.613553717771449 2305 76-77 0.5934357753069257 2305 78-79 0.48657620062355633 2305 80-81 0.3575093357721535 2305 82-83 0.12503602393615765 2305 84-85 0.22479086010855553 2305 86-87 0.40647878940850646 2305 88-89 0.31480433530601815 2305 90-91 0.30245875205685024 2305 92-93 0.34208030169120107 2306 1 0.41589112300189157 2306 2 0.4896287639626564 2306 3 0.5255794977194768 2306 4 0.5548981810978546 2306 5 0.5388213410048763 2306 6 0.5372759147459334 2306 7 0.6616677346439204 2306 8 0.8058850773649411 2306 9 0.71104473826027 2306 10-11 0.8191427254590451 2306 12-13 0.783675104636977 2306 14-15 1.034816171626595 2306 16-17 1.0847718311163277 2306 18-19 1.0826341467625298 2306 20-21 1.0169007657564464 2306 22-23 0.981649683219338 2306 24-25 1.041622608792224 2306 26-27 1.0164837261301543 2306 28-29 1.101978167070655 2306 30-31 0.9033104167010109 2306 32-33 0.9433579423266352 2306 34-35 1.0413725142671026 2306 36-37 0.8391873065689595 2306 38-39 0.7511099248659718 2306 40-41 1.0473606573812404 2306 42-43 1.1988725048640063 2306 44-45 1.0997114209958525 2306 46-47 1.1335889638859484 2306 48-49 0.9641767328157229 2306 50-51 1.1798480887906315 2306 52-53 1.0380084761126795 2306 54-55 1.0882293153399125 2306 56-57 1.2356344418811886 2306 58-59 0.9690948706725528 2306 60-61 0.9951003832737548 2306 62-63 0.9118095291882753 2306 64-65 0.8107711677615548 2306 66-67 0.9688234718220201 2306 68-69 1.012442370036684 2306 70-71 1.0689154413546582 2306 72-73 0.991712295162813 2306 74-75 0.9839533254282955 2306 76-77 0.8229018781249202 2306 78-79 0.8132035792633125 2306 80-81 0.7826846811614878 2306 82-83 0.7681686088385682 2306 84-85 0.6516363804667762 2306 86-87 0.6892477937623553 2306 88-89 0.5238570565204057 2306 90-91 0.6207526605017826 2306 92-93 0.7563432932808816 2307 1 -0.1016045716780738 2307 2 -0.06772500370524881 2307 3 -0.18037614367020183 2307 4 -0.18151552309601726 2307 5 -0.22114794816039307 2307 6 -0.15672727517677743 2307 7 0.035531724784945595 2307 8 -0.05983300352190213 2307 9 0.07781619453276534 2307 10-11 0.04823497845060842 2307 12-13 -0.025563414299973886 2307 14-15 0.1281209338097753 2307 16-17 0.2918619101305424 2307 18-19 0.18077662008811046 2307 20-21 0.17675103352206634 2307 22-23 0.17593249232561448 2307 24-25 0.01579008138264726 2307 26-27 0.01327255114753001 2307 28-29 0.01249759949793372 2307 30-31 -0.03560903449764652 2307 32-33 0.040090479182630645 2307 34-35 0.02251827891909386 2307 36-37 0.039822333141671606 2307 38-39 0.12009170420856918 2307 40-41 0.0508393073917901 2307 42-43 0.0927871899153061 2307 44-45 0.03311959413404253 2307 46-47 0.14117121083058493 2307 48-49 0.2607041809061812 2307 50-51 0.07033122580645923 2307 52-53 0.11703518102418542 2307 54-55 -0.009206931963323228 2307 56-57 -0.00214005832391706 2307 58-59 -0.04861417079878194 2307 60-61 -0.10093538265249791 2307 62-63 -0.005516615730194019 2307 64-65 0.07901544120665349 2307 66-67 0.07330970507707235 2307 68-69 -0.02979144676994494 2307 70-71 0.08345490157643454 2307 72-73 -0.05823349419385693 2307 74-75 -0.024927782333822535 2307 76-77 -0.1279696341928549 2307 78-79 -0.1482898621553197 2307 80-81 -0.2671067473026234 2307 82-83 -0.3320055383532825 2307 84-85 -0.14911801448145923 2307 86-87 -0.17202504906910931 2307 88-89 -0.03323682621683943 2307 90-91 0.03400977595774535 2307 92-93 -0.030779720683419498 2308 1 0.23520692889729133 2308 2 0.14040794249645927 2308 3 0.2856803904721019 2308 4 0.23667635199823422 2308 5 0.17921267688679876 2308 6 0.1786648841892884 2308 7 0.3541524833796714 2308 8 0.5168179798693657 2308 9 0.511705418397284 2308 10-11 0.5380654778487042 2308 12-13 0.3869926563911079 2308 14-15 0.7561416226015893 2308 16-17 0.8500495445859215 2308 18-19 0.753408853087933 2308 20-21 0.7445555428640844 2308 22-23 0.656777944089086 2308 24-25 0.5891804527575246 2308 26-27 0.61200159349346 2308 28-29 0.6441673021588343 2308 30-31 0.49556916849437727 2308 32-33 0.6423792832984105 2308 34-35 0.7993887293418425 2308 36-37 0.6664059467201255 2308 38-39 0.6571496414714844 2308 40-41 0.6489910050714869 2308 42-43 0.6684629583040973 2308 44-45 0.7144954982869081 2308 46-47 0.7021746326008511 2308 48-49 0.5037764911719407 2308 50-51 0.5824128944264544 2308 52-53 0.6284900670760294 2308 54-55 0.6524434045442504 2308 56-57 0.6784174428842817 2308 58-59 0.6679936502772996 2308 60-61 0.6111328050199845 2308 62-63 0.5046654577761274 2308 64-65 0.6676047853907647 2308 66-67 0.6878472732647083 2308 68-69 0.7271541709946661 2308 70-71 0.6926521286146041 2308 72-73 0.6241874356251991 2308 74-75 0.48631980601624747 2308 76-77 0.4685317145615855 2308 78-79 0.4834061504722236 2308 80-81 0.47756364120244754 2308 82-83 0.49414382883768937 2308 84-85 0.3842313428374524 2308 86-87 0.46128797876489713 2308 88-89 0.5347542200125623 2308 90-91 0.5915590638773303 2308 92-93 0.4936740739316683 2309 1 0.5684261616123472 2309 2 0.5211030833697521 2309 3 0.5776464196309625 2309 4 0.4597338762371237 2309 5 0.5691604396482219 2309 6 0.5265301653491505 2309 7 0.7892775553826326 2309 8 0.780201247138713 2309 9 0.7880066630421823 2309 10-11 0.683775742685917 2309 12-13 0.6986830037016034 2309 14-15 0.9349917423663925 2309 16-17 1.0907738253064068 2309 18-19 0.9444809092251347 2309 20-21 0.953330561200886 2309 22-23 0.9553566969844312 2309 24-25 0.7885255711572796 2309 26-27 0.9413562835710252 2309 28-29 0.9075057857400708 2309 30-31 0.9740987692496574 2309 32-33 0.9057424239019554 2309 34-35 1.0646796109076249 2309 36-37 0.8040456413770727 2309 38-39 0.6181455742510948 2309 40-41 0.703223123011675 2309 42-43 0.664125253350015 2309 44-45 0.6428649175189705 2309 46-47 0.7218464431778813 2309 48-49 0.6633756036379665 2309 50-51 0.7907635817483758 2309 52-53 0.6784526582141766 2309 54-55 0.8243881469689747 2309 56-57 0.8719165075513331 2309 58-59 0.5917807253038667 2309 60-61 0.5698668644778699 2309 62-63 0.707537235912838 2309 64-65 0.620763052875489 2309 66-67 0.5670912889608353 2309 68-69 0.7019562538297848 2309 70-71 0.7349263705522766 2309 72-73 0.6980305756702556 2309 74-75 0.571187271550631 2309 76-77 0.6180554998244681 2309 78-79 0.5519592823448072 2309 80-81 0.7469284644249647 2309 82-83 0.6727539511097866 2309 84-85 0.5353749904903893 2309 86-87 0.4738002664285723 2309 88-89 0.5459426038219881 2309 90-91 0.725945108478026 2309 92-93 0.8010059212090432 2310 1 0.387828035097435 2310 2 0.45153912232756355 2310 3 0.5275949463594358 2310 4 0.33756913052928894 2310 5 0.4485963351908495 2310 6 0.4935070442655558 2310 7 0.6079048448782487 2310 8 0.7357960012668698 2310 9 0.4862940585514366 2310 10-11 0.6528822305900519 2310 12-13 0.7503671026495624 2310 14-15 0.8976028713846844 2310 16-17 1.1307787099423834 2310 18-19 0.888699892851669 2310 20-21 0.8287521805429137 2310 22-23 0.7358999068434429 2310 24-25 0.601570176119445 2310 26-27 0.5543299619149096 2310 28-29 0.6952508872595615 2310 30-31 0.6345809992563645 2310 32-33 0.7121015383629015 2310 34-35 0.9244026279087123 2310 36-37 0.8023741800047404 2310 38-39 0.6869064102622247 2310 40-41 0.7874549430071554 2310 42-43 0.827504825064004 2310 44-45 0.8976013814685118 2310 46-47 0.8279620713150919 2310 48-49 0.7306604702121362 2310 50-51 0.8199126114239625 2310 52-53 0.7855868891467033 2310 54-55 0.9017997939858695 2310 56-57 0.9182045746980378 2310 58-59 0.7752601480648487 2310 60-61 0.7426170210276091 2310 62-63 0.7991022576594062 2310 64-65 0.8242387411618743 2310 66-67 0.7387683520450565 2310 68-69 0.6849397582437078 2310 70-71 0.7383888532821068 2310 72-73 0.7534529120881963 2310 74-75 0.7808242749400556 2310 76-77 0.7778343209516585 2310 78-79 0.5590548550754768 2310 80-81 0.6483406780028353 2310 82-83 0.7345108013147588 2310 84-85 0.6907713417809092 2310 86-87 0.6696049818062271 2310 88-89 0.6545920658730573 2310 90-91 0.6423075740264288 2310 92-93 0.925102192556249 2311 1 0.4901977673481639 2311 2 0.5569482728894855 2311 3 0.5948788220572752 2311 4 0.66708715390034 2311 5 0.7062449859083415 2311 6 0.6033039497216279 2311 7 0.5365069214318439 2311 8 0.44390281457816627 2311 9 0.6611079950300791 2311 10-11 0.5525137562745499 2311 12-13 0.5705133339809905 2311 14-15 0.6071265644869115 2311 16-17 0.7982591925913525 2311 18-19 0.6277886727505546 2311 20-21 0.6113902296198432 2311 22-23 0.7711829524553835 2311 24-25 0.6980818943885225 2311 26-27 0.5807964882353573 2311 28-29 0.49276109524963374 2311 30-31 0.4883023133417268 2311 32-33 0.4138118005472151 2311 34-35 0.6505969023533851 2311 36-37 0.5848350645351985 2311 38-39 0.4548544711006528 2311 40-41 0.5195024290431149 2311 42-43 0.36096092495837695 2311 44-45 0.5177138505403889 2311 46-47 0.4605004198574463 2311 48-49 0.3510653320939383 2311 50-51 0.40104250618757575 2311 52-53 0.40582061129902414 2311 54-55 0.47558802719809634 2311 56-57 0.3397945792064654 2311 58-59 0.27972573078834984 2311 60-61 0.31218939581546934 2311 62-63 0.18903010247176155 2311 64-65 0.28135185258984663 2311 66-67 0.374249343456345 2311 68-69 0.5587465024878959 2311 70-71 0.5538236195031985 2311 72-73 0.5741606156171386 2311 74-75 0.43410801849939773 2311 76-77 0.5250164965741995 2311 78-79 0.40709913253256147 2311 80-81 0.23469423463858874 2311 82-83 0.050392044313088746 2311 84-85 0.28591234141738653 2311 86-87 0.12319107222097614 2311 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92-93 -0.27990168710140395 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 87943.0 3 9459.0 4 4378.0 5 4253.0 6 3522.0 7 12271.0 8 21696.0 9 17395.0 10 17770.0 11 27188.0 12 44052.0 13 55682.0 14 59326.0 15 60001.0 16 61438.0 17 62958.0 18 63496.0 19 63964.0 20 64518.0 21 65721.0 22 68517.0 23 72831.0 24 77826.0 25 81650.0 26 88489.0 27 93893.0 28 100766.0 29 110185.0 30 121114.0 31 137857.0 32 158382.0 33 184592.0 34 224287.0 35 295628.0 36 438385.0 37 802059.0 38 1053101.0 39 48130.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.49546976204487 22.76716303995979 10.926762273423465 24.810604924571873 2 16.577216789613725 22.219899475376266 37.61325975532998 23.589623979680034 3 16.396230601509693 26.44295349155265 30.93616838420528 26.22464752273237 4 12.489381603146203 16.771421086758213 40.976968890755046 29.76222841934054 5 13.180769916732043 37.22559048908197 37.133776150746236 12.45986344343975 6 29.683411135973508 40.228609495604736 18.088680993785133 11.999298374636615 7 26.990122249630645 32.01640987758548 25.400023744195867 15.593444128588011 8 24.14860559879192 36.454822546774935 22.48139998033805 16.91517187409509 9 24.474250608172184 14.988826124603142 24.050344287304664 36.48657897992001 10-11 23.566140840706215 26.123454414498752 30.93186938793001 19.37853535686502 12-13 23.024670225791628 24.696297255813906 30.632983486359254 21.646049032035208 14-15 21.142412718941202 25.97827041372338 26.479976481053548 26.39934038628187 16-17 21.205843960926433 30.280649214666123 27.594031665463685 20.919475158943754 18-19 21.352806184390268 29.154570124680962 29.005494507182554 20.487129183746223 20-21 21.511371396150125 28.67366693593414 28.864479688732008 20.950481979183728 22-23 21.794268308996376 28.468869884149168 28.77376108792415 20.96310071893031 24-25 21.2336888012388 28.37023976668839 29.08981330840053 21.306258123672283 26-27 21.626246336071027 28.92583185313569 28.776935906027905 20.670985904765374 28-29 21.666024483189 28.732648749523282 28.677870723236666 20.923456044051058 30-31 21.377073491970485 28.59686493717948 28.911408289301065 21.114653281548968 32-33 21.51911516583383 28.854618243059004 28.85860979217196 20.7676567989352 34-35 21.53677545962347 28.617703840522452 28.85016707002469 20.995353629829392 36-37 21.47706764927659 28.742784684812296 28.919198889684072 20.86094877622704 38-39 21.51061890088902 28.680010852484962 29.003513450772722 20.805856795853295 40-41 21.374324540492857 28.645859030605585 29.08916626864328 20.890650160258275 42-43 21.267349274806463 28.691348257243725 29.163380872763806 20.877921595186006 44-45 21.29391918633279 28.55682830976345 29.093927615773747 21.055324888130013 46-47 21.322653287108455 28.57843290430595 29.26957553918973 20.829338269395866 48-49 21.334197748875457 28.696433584565273 29.09806502794877 20.8713036386105 50-51 21.10704704605518 28.670517708195533 29.25431778612105 20.968117459628242 52-53 21.287922444543327 28.466116381120504 29.40505802799641 20.84090314633976 54-55 21.263497950503325 28.68414234022763 29.2463912354686 20.805968473800444 56-57 21.2793272389422 28.57171017271775 29.130768119342864 21.01819446899718 58-59 21.278677762407835 28.660153784773097 29.117873046235122 20.943295406583946 60-61 21.08419143530612 28.608816608342856 29.315006493164454 20.99198546318657 62-63 20.984613751889476 28.691327518413225 29.254073179080535 21.069985550616764 64-65 21.19114106152196 28.501838619483788 29.321021596338877 20.985998722655374 66-67 21.015627728594925 28.493838468843503 29.326200249138907 21.164333553422665 68-69 21.120754776849797 28.634978236165576 29.165072802066334 21.079194184918297 70-71 21.24899443399758 28.505102849864823 29.155988642702862 21.089914073434738 72-73 21.203497122694166 28.55239080524693 29.08789861982351 21.156213452235395 74-75 21.059627133556166 28.62763255966192 29.212253568424494 21.100486738357425 76-77 21.152167363240963 28.615381371034438 29.118764786587693 21.11368647913691 78-79 21.364910261180952 28.50558534023029 29.056327136086967 21.073177262501797 80-81 21.285657247732168 28.707776365890247 28.952417840665763 21.054148545711822 82-83 21.331356662978866 28.683148832500073 28.958326149132102 21.027168355388962 84-85 21.25198072470774 28.636405864467257 29.041741263685307 21.0698721471397 86-87 21.174485510847344 28.568058240512844 29.1022355313874 21.155220717252412 88-89 21.288155825996842 28.509693516340583 29.115630051228298 21.08652060643427 90-91 21.538374989153393 28.586044431859843 28.94700295235339 20.928577626633373 92-93 21.65337482576958 28.454273805534246 28.856742840301603 21.035608528394572 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 83680.0 1 44395.5 2 5088.5 3 4814.5 4 4152.5 5 3373.5 6 2823.5 7 2598.0 8 2425.0 9 2332.0 10 2513.5 11 2710.5 12 3004.5 13 3519.0 14 3791.0 15 4169.5 16 5057.0 17 6327.5 18 8213.5 19 10840.0 20 14497.0 21 19502.0 22 26285.0 23 34856.5 24 45257.5 25 57243.5 26 71085.5 27 86925.5 28 103625.5 29 112104.0 30 120490.5 31 136367.0 32 150913.5 33 164351.5 34 176274.5 35 185833.0 36 192350.5 37 196276.5 38 196602.0 39 194275.5 40 190552.5 41 184909.5 42 178450.5 43 175138.0 44 171804.5 45 162283.0 46 151403.5 47 141518.5 48 131705.0 49 123030.5 50 114248.0 51 105440.5 52 96993.0 53 88637.5 54 81329.0 55 73879.5 56 66299.5 57 62848.0 58 60536.5 59 56034.0 60 51720.5 61 47326.5 62 43019.0 63 38611.0 64 33146.5 65 28800.0 66 25999.0 67 22914.5 68 20495.5 69 18670.5 70 16552.5 71 15378.0 72 14260.5 73 12126.5 74 10861.0 75 10106.5 76 9353.5 77 9043.0 78 7618.0 79 6751.5 80 6204.5 81 4573.5 82 3490.0 83 2709.0 84 2283.0 85 1758.0 86 1373.0 87 1151.5 88 846.5 89 641.0 90 419.5 91 284.5 92 214.5 93 144.0 94 94.0 95 70.0 96 53.5 97 38.5 98 27.5 99 45.5 100 66.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.618232678842304 2 2.588160507645643 3 2.503039948049468 4 2.427849449002492 5 2.310662649255558 6 2.2786971196580352 7 2.2762800663803398 8 2.2702575752967484 9 2.298678093420318 10-11 2.2337399286929 12-13 2.037737049982446 14-15 1.8199404075514385 16-17 1.7296936807954844 18-19 1.6577259194521026 20-21 1.9826685194722848 22-23 2.2032347021173186 24-25 2.4110207155565373 26-27 2.6821435959267013 28-29 2.7422375830434045 30-31 2.889345488157144 32-33 2.8608645437016325 34-35 2.5747458619544332 36-37 2.38028385470851 38-39 2.3006520202637692 40-41 2.226257134587368 42-43 2.1361614736612697 44-45 2.0306873112558343 46-47 2.022006061566778 48-49 2.351510849648611 50-51 2.6309524217161764 52-53 3.3558367707523664 54-55 3.6454702508075476 56-57 3.949384487311779 58-59 4.028663834820191 60-61 3.8287433155888055 62-63 3.585567613741995 64-65 3.3849521916932725 66-67 3.1130639111185054 68-69 2.877250150713343 70-71 2.6386668500941544 72-73 3.003329692311132 74-75 2.862425557276811 76-77 3.531556544040826 78-79 4.164915142294948 80-81 4.678760527022353 82-83 4.500079863468717 84-85 4.061263840903108 86-87 3.6534968819005615 88-89 3.2828115485999927 90-91 2.971948283922386 92-93 2.782441235895739 >>END_MODULE >>Sequence Length Distribution pass #Length Count 93 4964723.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.29373018008218 #Duplication Level Percentage of deduplicated Percentage of total 1 93.37820674307167 77.77819157157343 2 3.9490844295819816 6.578679458719307 3 1.1236215765972764 2.8077189727683622 4 0.5634960808127959 1.877427620510192 5 0.2594930016614937 1.0807070032006036 6 0.1795304077001856 0.8972254402859645 7 0.10759494622938336 0.6273389093979496 8 0.09052719482703178 0.6032278191906013 9 0.060811444800088994 0.4558690867535617 >10 0.269505163699641 3.7429257587753546 >50 0.012466711828111179 0.7125182076076254 >100 0.005103014579232141 0.8047013235538377 >500 3.1978023207858435E-4 0.18622671294558343 >1k 2.1556622047771938E-4 0.2453639824197026 >5k 0.0 0.0 >10k+ 2.3938158563453986E-5 1.6018781322980185 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 80339 1.6181970273064579 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 2.0142110647462104E-5 0.0 2.0142110647462104E-5 5 6.042633194238631E-5 0.0 4.028422129492421E-5 6 6.042633194238631E-5 0.0 4.028422129492421E-5 7 8.056844258984842E-5 0.0 4.028422129492421E-5 8 8.056844258984842E-5 0.0 4.028422129492421E-5 9 1.0071055323731053E-4 0.0 4.028422129492421E-5 10-11 1.0071055323731053E-4 0.0 6.042633194238631E-5 12-13 1.1078160856104157E-4 1.0071055323731052E-5 9.063949791357948E-5 14-15 1.2085266388477262E-4 2.0142110647462104E-5 1.1078160856104157E-4 16-17 1.2085266388477262E-4 2.0142110647462104E-5 1.712079405034279E-4 18-19 1.3092371920850367E-4 2.0142110647462104E-5 2.719184937407384E-4 20-21 1.4099477453223473E-4 2.0142110647462104E-5 3.2227377035939366E-4 22-23 1.6113688517969683E-4 2.0142110647462104E-5 4.7333960021535943E-4 24-25 1.8127899582715893E-4 2.0142110647462104E-5 0.0011380292515816089 26-27 1.9135005115089E-4 2.0142110647462104E-5 0.0026084033288463425 28-29 2.8198954906446947E-4 3.0213165971193156E-5 0.010161694821644631 30-31 3.524869363305868E-4 4.028422129492421E-5 0.018994010340556762 32-33 3.6255799165431786E-4 6.042633194238631E-5 0.030565652907523742 34-35 3.8270010230178E-4 8.056844258984842E-5 0.05128181370843852 36-37 4.028422129492421E-4 8.056844258984842E-5 0.10278519063399912 38-39 4.229843235967042E-4 8.056844258984842E-5 0.1955597522762096 40-41 4.229843235967042E-4 8.056844258984842E-5 0.30400487600214554 42-43 4.531974895678973E-4 8.056844258984842E-5 0.41777758799433523 44-45 4.6326854489162837E-4 9.063949791357948E-5 0.5530620741580144 46-47 4.7333960021535943E-4 1.0071055323731053E-4 0.7157499018575659 48-49 4.934817108628216E-4 1.0071055323731053E-4 0.8796663983066124 50-51 5.035527661865526E-4 1.2085266388477262E-4 1.0420923785677467 52-53 5.236948768340147E-4 1.2085266388477262E-4 1.2041658718925508 54-55 5.438369874814768E-4 1.2085266388477262E-4 1.3726244142926 56-57 5.438369874814768E-4 1.2085266388477262E-4 1.5598251906501126 58-59 5.539080428052078E-4 1.3092371920850367E-4 1.754236842619417 60-61 6.042633194238631E-4 1.4099477453223473E-4 1.9491621184102317 62-63 6.344764853950563E-4 1.5106582985596577E-4 2.1329588780683233 64-65 6.546185960425183E-4 1.6113688517969683E-4 2.312817855094836 66-67 7.150449279849047E-4 1.6113688517969683E-4 2.5126578058836313 68-69 7.452580939560978E-4 1.6113688517969683E-4 2.720463961433498 70-71 7.6540020460356E-4 1.8127899582715893E-4 2.9208175360438036 72-73 7.855423152510221E-4 1.8127899582715893E-4 3.1140005192636124 74-75 8.056844258984842E-4 1.8127899582715893E-4 3.300687671799615 76-77 8.056844258984842E-4 2.0142110647462105E-4 3.496327992518414 78-79 8.358975918696773E-4 2.0142110647462105E-4 3.693086200378148 80-81 8.761818131646016E-4 2.0142110647462105E-4 3.893228282826655 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACTG 1830 0.0 15.084961 5 GTGTAAG 1620 0.0 14.688517 1 TAGTGCG 675 0.0 14.68842 9 ACTGTGC 1240 0.0 13.905874 8 GTATAAG 2055 0.0 13.895122 1 TATACAG 1785 0.0 13.773742 5 ATTATAC 2755 0.0 13.642379 3 GTATTAG 1880 0.0 13.577643 1 TACCGTG 645 0.0 13.368541 7 GTACTAT 1490 0.0 13.35678 1 CAGTGCG 530 4.838512E-10 13.013531 9 GTATATA 3730 0.0 12.990906 1 CTAATAC 1970 0.0 12.719038 3 GTATAAT 2825 0.0 12.711297 1 GTATAGA 2420 0.0 12.693262 1 GTCTTAG 1535 0.0 12.683363 1 TACCCCG 820 0.0 12.624456 5 TTATACT 3265 0.0 12.559937 4 GTATTAA 2675 0.0 12.453666 1 ATACTGT 2010 0.0 12.226986 6 >>END_MODULE