FastQCFastQC Report
Wed 5 Nov 2014
lane1_Undetermined_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane1_Undetermined_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4964723
Sequences flagged as poor quality0
Sequence length93
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN803391.6181970273064579No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG18300.015.0849615
GTGTAAG16200.014.6885171
TAGTGCG6750.014.688429
ACTGTGC12400.013.9058748
GTATAAG20550.013.8951221
TATACAG17850.013.7737425
ATTATAC27550.013.6423793
GTATTAG18800.013.5776431
TACCGTG6450.013.3685417
GTACTAT14900.013.356781
CAGTGCG5304.838512E-1013.0135319
GTATATA37300.012.9909061
CTAATAC19700.012.7190383
GTATAAT28250.012.7112971
GTATAGA24200.012.6932621
GTCTTAG15350.012.6833631
TACCCCG8200.012.6244565
TTATACT32650.012.5599374
GTATTAA26750.012.4536661
ATACTGT20100.012.2269866
Produced by FastQC (version 0.11.2)