##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename lane1_Undetermined_L001_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4964723 Sequences flagged as poor quality 0 Sequence length 93 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01339007231622 33.0 33.0 33.0 27.0 33.0 2 32.157183794544025 33.0 33.0 33.0 33.0 33.0 3 32.25822205992157 33.0 33.0 33.0 33.0 33.0 4 36.18994191619553 37.0 37.0 37.0 33.0 37.0 5 35.87549698140259 37.0 37.0 37.0 33.0 37.0 6 35.78915439995343 37.0 37.0 37.0 33.0 37.0 7 35.69483735547784 37.0 37.0 37.0 33.0 37.0 8 35.657500730655066 37.0 37.0 37.0 33.0 37.0 9 35.72781986024195 37.0 37.0 37.0 33.0 37.0 10-11 35.66877517637943 37.0 37.0 37.0 33.0 37.0 12-13 35.59281393946853 37.0 37.0 37.0 33.0 37.0 14-15 37.301021829415255 40.0 37.0 40.0 33.0 40.0 16-17 37.16333559797798 40.0 37.0 40.0 33.0 40.0 18-19 37.16958911907069 40.0 37.0 40.0 33.0 40.0 20-21 37.0963765752893 40.0 37.0 40.0 33.0 40.0 22-23 37.00489886344113 40.0 37.0 40.0 33.0 40.0 24-25 36.75991409792651 40.0 37.0 40.0 33.0 40.0 26-27 35.74448715467106 38.5 37.0 40.0 27.0 40.0 28-29 34.99980331228953 37.0 35.0 40.0 24.5 40.0 30-31 34.625456848247126 37.0 33.0 40.0 22.0 40.0 32-33 34.431913220536174 37.0 33.0 40.0 22.0 40.0 34-35 34.27363691791062 37.0 33.0 40.0 22.0 40.0 36-37 34.0540726038492 37.0 33.0 40.0 22.0 40.0 38-39 33.853039937978416 37.0 33.0 40.0 15.0 40.0 40-41 33.71721604609159 37.0 33.0 40.0 15.0 40.0 42-43 33.63664991984447 37.0 33.0 40.0 15.0 40.0 44-45 33.46389979863932 37.0 33.0 40.0 15.0 40.0 46-47 33.355092922606154 37.0 33.0 40.0 15.0 40.0 48-49 33.15131569273855 37.0 33.0 40.0 15.0 40.0 50-51 32.893277530287186 37.0 33.0 40.0 15.0 40.0 52-53 32.709298685948845 37.0 33.0 40.0 15.0 40.0 54-55 32.289214524153714 37.0 33.0 40.0 15.0 40.0 56-57 32.144417019841796 37.0 33.0 40.0 15.0 40.0 58-59 31.92491353495452 37.0 33.0 40.0 15.0 40.0 60-61 31.68562344767271 37.0 30.0 40.0 10.5 40.0 62-63 31.43211977788086 37.0 27.0 40.0 6.0 40.0 64-65 31.139360342963748 37.0 27.0 37.0 6.0 40.0 66-67 30.92122188891505 37.0 27.0 37.0 6.0 40.0 68-69 30.694788410148966 37.0 27.0 37.0 6.0 40.0 70-71 30.483807455118843 37.0 27.0 37.0 6.0 40.0 72-73 30.23288691030698 35.0 27.0 37.0 6.0 40.0 74-75 29.954735541136937 33.0 27.0 37.0 6.0 40.0 76-77 28.57208720405952 33.0 24.5 37.0 6.0 37.0 78-79 29.580648809611333 33.0 27.0 37.0 6.0 37.0 80-81 29.676578230044257 33.0 27.0 37.0 6.0 37.0 82-83 29.486286243965676 33.0 27.0 37.0 6.0 37.0 84-85 29.33723230883173 33.0 27.0 37.0 6.0 37.0 86-87 29.09253255418278 33.0 27.0 37.0 6.0 37.0 88-89 28.92517427457685 33.0 27.0 37.0 6.0 37.0 90-91 28.70313993348672 33.0 27.0 37.0 6.0 37.0 92-93 27.583541518831968 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2304 1 0.20318160717346245 2304 2 0.18699689184468582 2304 3 0.21804894850725276 2304 4 0.16687213027164916 2304 5 0.1508417450951356 2304 6 0.277995823345492 2304 7 0.30077959387309505 2304 8 0.28115345861695573 2304 9 0.3841726843710802 2304 10-11 0.3630591902262239 2304 12-13 0.4684152671185231 2304 14-15 0.6631496231628233 2304 16-17 0.8232120888669456 2304 18-19 0.6867988358898245 2304 20-21 0.655120561077922 2304 22-23 0.5793360932215705 2304 24-25 0.6937793534371508 2304 26-27 0.05929943509371327 2304 28-29 -0.18168930319547627 2304 30-31 -0.4635041258996182 2304 32-33 -0.5390097658315653 2304 34-35 -0.6476709879402165 2304 36-37 -0.5642881585203057 2304 38-39 -0.4295276489202351 2304 40-41 -0.548648889535535 2304 42-43 -0.6948046551107225 2304 44-45 -0.8942535067098234 2304 46-47 -0.7744183718932902 2304 48-49 -0.9671594774023475 2304 50-51 -1.0934146698373084 2304 52-53 -0.9788359132828006 2304 54-55 -0.8313383124532976 2304 56-57 -0.9360796534556606 2304 58-59 -0.8696429578403446 2304 60-61 -0.9178311312189784 2304 62-63 -0.970034846652382 2304 64-65 -0.9164056947119157 2304 66-67 -0.7143142864688379 2304 68-69 -0.7864393333841981 2304 70-71 -0.7382689269738094 2304 72-73 -0.752682543428925 2304 74-75 -0.6744579776338426 2304 76-77 -0.8124394493808538 2304 78-79 -0.424516584557427 2304 80-81 -0.38907060758937106 2304 82-83 -0.4412926679155973 2304 84-85 -0.46752739207858696 2304 86-87 -0.47736474754622193 2304 88-89 -0.48324263019236113 2304 90-91 -0.315897625994463 2304 92-93 -0.04212767375517146 2305 1 0.13858943584224193 2305 2 0.14532480982214224 2305 3 0.07204678631331518 2305 4 0.13906533349825168 2305 5 0.04135109125904535 2305 6 0.1990137764429747 2305 7 0.19884323809666427 2305 8 0.12853266218071013 2305 9 0.2744160611577797 2305 10-11 0.2659871675833969 2305 12-13 0.31461636343881594 2305 14-15 0.54122980903648 2305 16-17 0.589871286455427 2305 18-19 0.40577707257537554 2305 20-21 0.5631892055257737 2305 22-23 0.5668282388990846 2305 24-25 0.7934754244717084 2305 26-27 0.4850162719204647 2305 28-29 0.1953364923244152 2305 30-31 -0.12517067795268133 2305 32-33 -0.0846330560186388 2305 34-35 -0.10115749550564601 2305 36-37 -0.28067398894415163 2305 38-39 0.005004455009405717 2305 40-41 -0.2299222653647348 2305 42-43 -0.33217235453921745 2305 44-45 -0.29019372820034306 2305 46-47 -0.3320624908277736 2305 48-49 -0.2822201661303225 2305 50-51 -0.3330465528643032 2305 52-53 -0.5100844086364518 2305 54-55 -0.032122050175246386 2305 56-57 0.1401464571474591 2305 58-59 0.05996284483275005 2305 60-61 -0.0621160634998823 2305 62-63 -0.23820326964920113 2305 64-65 -0.1263500758538214 2305 66-67 -0.12326973314329237 2305 68-69 -0.1407278181385614 2305 70-71 -0.1901325826258322 2305 72-73 -0.029910047930936656 2305 74-75 -0.13744370489447633 2305 76-77 -0.045156622151598214 2305 78-79 -0.03448241873445923 2305 80-81 -0.028258634338982347 2305 82-83 0.20557123506052832 2305 84-85 0.2703702609308536 2305 86-87 0.2995799519911877 2305 88-89 0.4611630796053241 2305 90-91 0.39397952247600543 2305 92-93 0.5915512771870226 2306 1 0.2500089415068132 2306 2 0.173598534038085 2306 3 0.19297016030328962 2306 4 0.2251745535106835 2306 5 0.23751729626351192 2306 6 0.36654308365218924 2306 7 0.39053546687411966 2306 8 0.38986497416581756 2306 9 0.32040771870120466 2306 10-11 0.3054215588198872 2306 12-13 0.40378859520346566 2306 14-15 0.5163055244269756 2306 16-17 0.6896251320308409 2306 18-19 0.575110317491017 2306 20-21 0.6596957669754033 2306 22-23 0.7298199422007343 2306 24-25 0.7980338416546502 2306 26-27 0.7561366076680329 2306 28-29 0.45949992621944347 2306 30-31 0.3546927105249864 2306 32-33 0.07692769586635961 2306 34-35 0.07743424832766266 2306 36-37 0.18650600643044868 2306 38-39 0.1779790934910821 2306 40-41 0.04634483702758985 2306 42-43 0.1154934650323014 2306 44-45 0.061171183674801455 2306 46-47 0.006720408737272976 2306 48-49 0.1930304220900254 2306 50-51 0.07752040385575754 2306 52-53 0.05463460026739142 2306 54-55 0.18235384624426132 2306 56-57 0.2660611437899121 2306 58-59 0.34880782786760633 2306 60-61 0.11499184357188952 2306 62-63 0.20724611685497507 2306 64-65 0.2977939536644634 2306 66-67 0.3243509479600384 2306 68-69 0.25658175356141655 2306 70-71 0.25172783084460093 2306 72-73 0.20008248098118386 2306 74-75 0.3792891355928951 2306 76-77 0.3182385694756569 2306 78-79 0.4091185113452269 2306 80-81 0.4393153155383125 2306 82-83 0.3734322118838378 2306 84-85 0.5015887589023578 2306 86-87 0.6260589004735309 2306 88-89 0.6645990053431454 2306 90-91 0.8018460729188597 2306 92-93 0.8278645344377615 2307 1 0.18136757152114313 2307 2 0.2735631511731924 2307 3 0.2060079523084255 2307 4 0.2169425508674223 2307 5 0.15282048508457535 2307 6 0.2123406723259933 2307 7 0.28564674160363523 2307 8 0.28951642822460855 2307 9 0.26784104706989353 2307 10-11 0.31771539117623604 2307 12-13 0.40678828839005376 2307 14-15 0.5530224681871303 2307 16-17 0.6872439233421161 2307 18-19 0.6387001090790889 2307 20-21 0.5919526741590886 2307 22-23 0.4945985772306045 2307 24-25 0.5453054185446291 2307 26-27 0.616830174275556 2307 28-29 0.37122861294437115 2307 30-31 0.22827725497037932 2307 32-33 0.060562405216380455 2307 34-35 -0.09411808443886116 2307 36-37 -0.0028493548976484817 2307 38-39 -0.08494131223822166 2307 40-41 -0.23479800435265474 2307 42-43 -0.15239806406349032 2307 44-45 -0.3686775808178382 2307 46-47 -0.31841901701245234 2307 48-49 -0.2521165954918487 2307 50-51 -0.2953079066692652 2307 52-53 -0.18642962158412502 2307 54-55 -0.07810689087649791 2307 56-57 -0.04680366339255926 2307 58-59 -0.10348290252441572 2307 60-61 -0.055709140512416155 2307 62-63 -0.07584148093475207 2307 64-65 -0.0749344711804305 2307 66-67 -0.0714868942525122 2307 68-69 -0.16634787825640984 2307 70-71 -0.1015350739038432 2307 72-73 -0.1384345562716227 2307 74-75 -0.10419810901009541 2307 76-77 -0.12166659681296466 2307 78-79 0.0752072318621444 2307 80-81 0.10483751119471307 2307 82-83 0.03734595749795133 2307 84-85 0.138963230620746 2307 86-87 0.1659186215968731 2307 88-89 0.1780297908991315 2307 90-91 0.35127942227958897 2307 92-93 0.27375997898282023 2308 1 0.2268200955743893 2308 2 0.24874827360883245 2308 3 0.1748243553851907 2308 4 0.14056099240004016 2308 5 0.20762383515823757 2308 6 0.11874114214904097 2308 7 0.23312389908105047 2308 8 0.2808742638720645 2308 9 0.2602245833637937 2308 10-11 0.24412785027257655 2308 12-13 0.3280658801914669 2308 14-15 0.35081877176875054 2308 16-17 0.559485170058899 2308 18-19 0.4704575737172121 2308 20-21 0.5086947847197791 2308 22-23 0.5174878989619955 2308 24-25 0.6748462646126256 2308 26-27 0.6418395826109773 2308 28-29 0.4256260987981122 2308 30-31 0.2613188477680026 2308 32-33 0.1904036816114285 2308 34-35 0.04917942051482527 2308 36-37 0.23965154790673893 2308 38-39 0.29410827863403455 2308 40-41 0.32031571019476246 2308 42-43 0.2805515680277111 2308 44-45 0.0022889130903962496 2308 46-47 0.17802451966484512 2308 48-49 0.2556719695447214 2308 50-51 0.23558666867351974 2308 52-53 0.2684906565005889 2308 54-55 0.4274149424764815 2308 56-57 0.5530520508877217 2308 58-59 0.6140769280083553 2308 60-61 0.5941342686197508 2308 62-63 0.35789655763472794 2308 64-65 0.32633571036842923 2308 66-67 0.5550874060634179 2308 68-69 0.2946711121843819 2308 70-71 0.2892595499532753 2308 72-73 0.23951967851153455 2308 74-75 0.29629996426991667 2308 76-77 0.21715800426313692 2308 78-79 0.49819112977450786 2308 80-81 0.5943928812956152 2308 82-83 0.3886767955495536 2308 84-85 0.527665086777251 2308 86-87 0.5590495549697181 2308 88-89 0.7335711394176343 2308 90-91 0.7599880678044748 2308 92-93 0.8872120088122699 2309 1 0.23538595997681 2309 2 0.1796296641222952 2309 3 0.15310901981648328 2309 4 0.16068387052216337 2309 5 0.21849587382757818 2309 6 0.21584792280047083 2309 7 0.3433590587471542 2309 8 0.22340297816283083 2309 9 0.33473394575399595 2309 10-11 0.19656931880918194 2309 12-13 0.3203935729091114 2309 14-15 0.530436972631982 2309 16-17 0.6389238202681398 2309 18-19 0.6453591164447161 2309 20-21 0.41073224943127684 2309 22-23 0.5588969170747973 2309 24-25 0.521852003460296 2309 26-27 0.7932114933905794 2309 28-29 0.6586426834677965 2309 30-31 0.5983351505158367 2309 32-33 0.45559612075834366 2309 34-35 0.5024357590777271 2309 36-37 0.611859845678012 2309 38-39 0.7409865436471179 2309 40-41 0.5619530434219442 2309 42-43 0.49626453028376005 2309 44-45 0.5846174851428216 2309 46-47 0.729195082091529 2309 48-49 0.657752249838623 2309 50-51 0.6037529600880305 2309 52-53 0.8930879375142879 2309 54-55 1.1849820118051966 2309 56-57 1.1779013236986628 2309 58-59 1.1949244448233962 2309 60-61 1.1666805384133276 2309 62-63 1.2250271055761495 2309 64-65 1.2702885905017318 2309 66-67 1.3264266106397606 2309 68-69 1.2693011900084734 2309 70-71 1.208264091004395 2309 72-73 1.2191319602040487 2309 74-75 1.131743564896265 2309 76-77 1.0554392790965466 2309 78-79 1.2672973884694478 2309 80-81 1.1906107837197588 2309 82-83 1.3111213254188598 2309 84-85 1.2523311846489946 2309 86-87 1.4022202356312725 2309 88-89 1.3919463872132845 2309 90-91 1.4246436151443262 2309 92-93 1.5012344351167393 2310 1 0.16714354855698588 2310 2 0.17218364715954237 2310 3 0.11459387815494182 2310 4 0.115369997162027 2310 5 0.16264027445860307 2310 6 0.16197436387457742 2310 7 0.1839030992782682 2310 8 0.27401881148314544 2310 9 0.2254663429600967 2310 10-11 0.29152284376981186 2310 12-13 0.32129847302256564 2310 14-15 0.4049982783633439 2310 16-17 0.4910378097803729 2310 18-19 0.4206234760675187 2310 20-21 0.5655589158518168 2310 22-23 0.5660400582081238 2310 24-25 0.6493264833672612 2310 26-27 0.6893877747910508 2310 28-29 0.5782192820856196 2310 30-31 0.4588163510571235 2310 32-33 0.46021700058919635 2310 34-35 0.3123450738229323 2310 36-37 0.39537561921833486 2310 38-39 0.27419521699986404 2310 40-41 0.3568207677637645 2310 42-43 0.25106421761025643 2310 44-45 0.2848506768122476 2310 46-47 0.47635769787088833 2310 48-49 0.5383439155704792 2310 50-51 0.37117630751202313 2310 52-53 0.3960002597185124 2310 54-55 0.66252209350926 2310 56-57 0.7093620002491434 2310 58-59 0.7920915334525596 2310 60-61 0.7227072065378088 2310 62-63 0.7172757633662208 2310 64-65 0.7616637291245745 2310 66-67 0.7609401491751377 2310 68-69 0.7923857098303024 2310 70-71 0.7235282589336371 2310 72-73 0.7313903375677384 2310 74-75 0.889041516331055 2310 76-77 0.8590685352261254 2310 78-79 0.9046788047014935 2310 80-81 0.8019637639866808 2310 82-83 0.9846008049779371 2310 84-85 1.0104814269638567 2310 86-87 1.0893811332683292 2310 88-89 1.126098867372999 2310 90-91 0.9847738034726028 2310 92-93 1.11255695740644 2311 1 0.14433260178464025 2311 2 0.09906961134419134 2311 3 0.06205951123077824 2311 4 0.07753327319557002 2311 5 0.1046923038469032 2311 6 0.08917373496517911 2311 7 0.108929694334023 2311 8 0.10420442532613805 2311 9 0.09135845682389743 2311 10-11 0.015247350717118024 2311 12-13 0.09616768064852721 2311 14-15 0.20680732426226456 2311 16-17 0.26458595257959416 2311 18-19 0.17324801150901692 2311 20-21 0.23417116183082953 2311 22-23 0.21380923146060837 2311 24-25 0.3509796116589001 2311 26-27 0.42240819601016 2311 28-29 0.3281598634193372 2311 30-31 0.2401262918014453 2311 32-33 0.291792659047843 2311 34-35 0.1438204059809962 2311 36-37 0.03930727509126797 2311 38-39 0.17162262013783192 2311 40-41 0.17092902644903063 2311 42-43 0.2415523412988776 2311 44-45 0.2701486971505105 2311 46-47 0.21098767660357254 2311 48-49 0.313560379678151 2311 50-51 0.018275125279934912 2311 52-53 0.13718197955054734 2311 54-55 0.587328593559171 2311 56-57 0.47914896912296 2311 58-59 0.6464410875142441 2311 60-61 0.6768951383345545 2311 62-63 0.6465177802653947 2311 64-65 0.6748607560665683 2311 66-67 0.7107362222168767 2311 68-69 0.7076876972340678 2311 70-71 0.6778910268451703 2311 72-73 0.6203405321227606 2311 74-75 0.7345428844302013 2311 76-77 0.5706798710794025 2311 78-79 0.7387270829863333 2311 80-81 0.5818824085833079 2311 82-83 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-0.9581278364531229 2303 22-23 -0.9519027238090771 2303 24-25 -1.088946166297788 2303 26-27 -0.801033704612685 2303 28-29 -0.9838778561631543 2303 30-31 -0.7630644565578422 2303 32-33 -0.33690965625362423 2303 34-35 -0.2667894154726369 2303 36-37 -0.05979258005067578 2303 38-39 -0.24633347089831403 2303 40-41 -0.5422992218549822 2303 42-43 -0.23945779028255032 2303 44-45 -0.3019334571187571 2303 46-47 -0.060777735465840976 2303 48-49 0.03156480185080568 2303 50-51 0.09272100239305558 2303 52-53 -0.43263905176696227 2303 54-55 -0.8728561060988227 2303 56-57 -1.0575263865002675 2303 58-59 -1.3169763208810679 2303 60-61 -1.0552650008764495 2303 62-63 -1.340775489298995 2303 64-65 -1.4685787658991494 2303 66-67 -1.5624007979086763 2303 68-69 -1.2525040860576304 2303 70-71 -1.2195102541465168 2303 72-73 -1.1171154128642051 2303 74-75 -1.1131263699735854 2303 76-77 -1.1181102018711933 2303 78-79 -1.0835895756537184 2303 80-81 -0.9044827696088795 2303 82-83 -1.090969529549561 2303 84-85 -0.9339552007677696 2303 86-87 -0.7141237482126108 2303 88-89 -0.5207753143795983 2303 90-91 -0.41225195209316823 2303 92-93 -0.7866321044974889 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 3.0 7 26.0 8 291.0 9 2484.0 10 13163.0 11 92968.0 12 78690.0 13 38674.0 14 13592.0 15 5604.0 16 6705.0 17 11330.0 18 17629.0 19 25998.0 20 37150.0 21 50123.0 22 68785.0 23 76250.0 24 87542.0 25 101926.0 26 116141.0 27 129094.0 28 143409.0 29 154721.0 30 165035.0 31 174120.0 32 185550.0 33 204417.0 34 236911.0 35 306659.0 36 473050.0 37 877725.0 38 1041461.0 39 27497.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.37951908151958 24.00749252664873 11.15623492462058 26.456753467211115 2 15.531359957040907 22.921641348369285 36.6199685259379 24.927030168651907 3 14.875029281593354 27.11289230033579 30.659031732485374 27.35304668558548 4 11.60924788754579 17.48385559476329 39.85575831723139 31.051138200459523 5 12.097412081197682 38.19721664229807 37.145395624287595 12.559975652216648 6 28.7651899209684 41.764404580074256 17.5762474563032 11.894158042654142 7 26.164944146934282 32.58133434634722 25.299095236531826 15.954626270186676 8 23.4143576590275 37.37604293331169 22.06644761449934 17.143151793161472 9 23.512792153761648 14.730469353476519 23.53406222260537 38.22267627015646 10-11 22.858667039429996 26.7840018466287 30.60857574531348 19.748755368627815 12-13 22.219970781854297 25.13313431585206 30.47418758307362 22.172707319220024 14-15 20.243093119193155 26.536545946269307 26.030958826907362 27.189402107630173 16-17 20.44508827582123 31.105441330765082 27.055759203484264 21.39371118992943 18-19 20.568985352324823 29.800018913740956 28.692346450989593 20.93864928294463 20-21 20.78712861114055 29.46288156293076 28.397968570379795 21.352021255548898 22-23 20.823145621618767 29.242729151253755 28.31884074902064 21.615284478106837 24-25 20.57964199165606 29.080497846287034 28.543431754172953 21.79642840788395 26-27 20.945878306429456 29.733339030114713 28.22244321330669 21.098339450149137 28-29 20.900498906420545 29.701732999335068 28.149153364586425 21.24861472965796 30-31 20.8214608828578 29.277729214988458 28.353948580216137 21.54686132193761 32-33 21.013821473515012 29.544790245656333 28.261476476000126 21.179911804828528 34-35 20.712807336528257 29.28115877716625 28.378552900457386 21.627480985848102 36-37 20.92568340609871 29.347674789682028 28.38338852977722 21.343253274442038 38-39 20.799009646176827 29.470340052006506 28.37735965853611 21.353290643280555 40-41 20.80519428744588 29.34398762945799 28.53278577455527 21.31803230854086 42-43 20.769527627734615 29.42380106556887 28.50429569896345 21.30237560773306 44-45 20.66086461606674 29.163614989625884 28.51897799044764 21.65654240385973 46-47 20.6218117600967 29.19770905778507 28.729455327938386 21.451023854179844 48-49 20.63108503852749 29.494359821756465 28.542305648282724 21.332249491433323 50-51 20.60836186981265 29.47367830169855 28.538647000185136 21.379312828303668 52-53 20.65454985580265 29.219844811516353 28.719543716707502 21.406061615973496 54-55 20.626577839360134 29.299058605002326 28.589752114331553 21.484611441305983 56-57 20.536036979582935 29.31869865794916 28.564036505705843 21.581227856762062 58-59 20.557171192167946 29.302524088454383 28.510980313296002 21.62932440608167 60-61 20.45177469562748 29.27649069930927 28.629652427034884 21.642082178028364 62-63 20.509642697881052 29.417147539570514 28.48985612451818 21.583353638030257 64-65 20.453501150885717 29.322610022684202 28.540491865801187 21.683396960628894 66-67 20.407789304017637 29.12080888595263 28.594553125373235 21.876848684656498 68-69 20.420631401863854 29.422234138944876 28.401967492002438 21.755166967188828 70-71 20.560836731195174 29.335655193624284 28.323500855890742 21.780007219289804 72-73 20.555161257417478 29.342563926870866 28.22556799376086 21.87670682195079 74-75 20.39387988626147 29.48739154187978 28.316119124333767 21.802609447524983 76-77 20.379507400349148 29.387964266375583 28.440131211955823 21.79239712131945 78-79 20.600729363261774 29.400542295310455 28.211858103507424 21.786870237920343 80-81 20.38537483076196 29.46411510138412 28.166292822076638 21.98421724577728 82-83 20.43980838857159 29.42710664896638 28.24970676649595 21.883378195966078 84-85 20.363623820396988 29.505960192740353 28.28418811064829 21.846227876214368 86-87 20.312919563597447 29.29992816883172 28.301491047429522 22.085661220141308 88-89 20.386997488218384 29.384188941556584 28.222420256187142 22.006393314037886 90-91 20.49470192048746 29.449244045418517 28.160971870666778 21.895082163427244 92-93 20.587421864749373 29.308404947486764 28.028247463525446 22.075925724238417 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 382.0 1 282.0 2 275.0 3 655.0 4 1601.0 5 3485.0 6 6877.0 7 11778.5 8 17368.5 9 22719.0 10 26417.0 11 28079.5 12 28160.5 13 26446.5 14 25111.0 15 23547.0 16 20658.0 17 18522.5 18 17446.0 19 17888.0 20 20051.0 21 23719.0 22 29495.5 23 37391.0 24 47106.5 25 58730.0 26 72291.0 27 87539.0 28 103873.0 29 112280.0 30 119997.5 31 135197.5 32 149554.5 33 162485.5 34 173373.0 35 182003.5 36 187607.5 37 190745.0 38 191337.0 39 188662.0 40 184331.5 41 178964.0 42 172684.5 43 169376.0 44 165585.0 45 156000.0 46 145965.0 47 136803.5 48 127510.0 49 118459.0 50 108816.5 51 99783.0 52 92448.0 53 84873.5 54 76626.5 55 69482.0 56 62634.0 57 58992.0 58 56553.0 59 52245.0 60 48082.0 61 44323.5 62 40437.0 63 35960.5 64 31693.5 65 28824.5 66 27220.0 67 24633.0 68 21193.0 69 17809.0 70 15502.0 71 14778.0 72 14098.0 73 12600.0 74 11060.5 75 10334.0 76 10737.0 77 10114.5 78 8078.5 79 6306.5 80 4717.0 81 4106.5 82 3625.5 83 2913.0 84 2565.0 85 1699.0 86 1162.0 87 989.0 88 690.5 89 427.5 90 208.5 91 118.0 92 78.0 93 41.5 94 30.5 95 21.0 96 14.5 97 13.5 98 19.5 99 65.0 100 106.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.21054548259792139 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0015811556858257752 20-21 0.02265987447839487 22-23 0.0 24-25 0.37257869170143026 26-27 2.414676508639052 28-29 3.671161512938385 30-31 4.523565564483658 32-33 4.765079542202052 34-35 4.862285368186704 36-37 5.072971845559158 38-39 4.987609580635214 40-41 5.015466119660654 42-43 4.904382379439901 44-45 4.872577986727557 46-47 4.785463358177284 48-49 4.812786131270566 50-51 4.911955813043346 52-53 5.475612637401926 54-55 6.0928474760827545 56-57 6.149124533231763 58-59 6.248304286059867 60-61 6.264387761411866 62-63 6.298025086193126 64-65 6.389389700090015 66-67 6.401928163968059 68-69 6.323192653447131 70-71 6.477843778998345 72-73 6.4037610960369795 74-75 6.3540906511803374 76-77 6.335439056720787 78-79 6.152478194654566 80-81 6.081144909796579 82-83 6.1870924118022295 84-85 6.278849796856743 86-87 6.273270432207396 88-89 6.0648096580614865 90-91 6.097087390374045 92-93 6.05018648573143 >>END_MODULE >>Sequence Length Distribution pass #Length Count 93 4964723.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.77148552319399 #Duplication Level Percentage of deduplicated Percentage of total 1 93.24014103203291 78.10865124645511 2 4.128899317228013 6.917680587597842 3 1.1346378404439794 2.8515089227446277 4 0.44629059341005134 1.495457039399511 5 0.2918138474347181 1.2222839747922514 6 0.1837737787484066 0.9237001467578881 7 0.09799773147560334 0.5746590880530051 8 0.08395214873668079 0.5626236970028707 9 0.056428520003465335 0.4254390851909003 >10 0.3122239393618515 4.549403607496416 >50 0.015918690563597553 0.8937737217440875 >100 0.007406994207578535 1.0361999125021029 >500 3.578143101032035E-4 0.21272474370696545 >1k 1.5775204301899902E-4 0.22589422655662095 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 2.0142110647462104E-5 6 2.0142110647462104E-5 0.0 2.0142110647462104E-5 7 2.0142110647462104E-5 0.0 2.0142110647462104E-5 8 4.028422129492421E-5 0.0 2.0142110647462104E-5 9 6.042633194238631E-5 0.0 4.028422129492421E-5 10-11 6.042633194238631E-5 0.0 6.042633194238631E-5 12-13 8.056844258984842E-5 0.0 9.063949791357948E-5 14-15 8.056844258984842E-5 0.0 1.1078160856104157E-4 16-17 1.2085266388477262E-4 2.0142110647462104E-5 1.6113688517969683E-4 18-19 1.2085266388477262E-4 2.0142110647462104E-5 2.9206060438820053E-4 20-21 1.2085266388477262E-4 3.0213165971193156E-5 3.6255799165431786E-4 22-23 1.2085266388477262E-4 4.028422129492421E-5 5.136238215102837E-4 24-25 1.2085266388477262E-4 4.028422129492421E-5 0.0011178871409341469 26-27 1.2085266388477262E-4 4.028422129492421E-5 0.0025781901628751493 28-29 1.2085266388477262E-4 4.028422129492421E-5 0.010252334319558212 30-31 1.712079405034279E-4 4.028422129492421E-5 0.019004081395880494 32-33 2.114921617983521E-4 4.028422129492421E-5 0.03051529763090509 34-35 2.517763830932763E-4 4.028422129492421E-5 0.05133216898505717 36-37 2.6184743841700734E-4 4.028422129492421E-5 0.10358080400457387 38-39 2.719184937407384E-4 4.028422129492421E-5 0.19686898946829462 40-41 2.9206060438820053E-4 4.028422129492421E-5 0.30584787912638833 42-43 3.2227377035939366E-4 4.028422129492421E-5 0.4188451198586507 44-45 3.424158810068558E-4 4.028422129492421E-5 0.554240387630891 46-47 3.9277115762551104E-4 5.0355276618655256E-5 0.7165052310068457 48-49 4.229843235967042E-4 6.042633194238631E-5 0.8807339301709279 50-51 4.431264342441663E-4 6.042633194238631E-5 1.0418708153506249 52-53 4.834106555390905E-4 6.042633194238631E-5 1.2013761895678772 54-55 5.236948768340147E-4 9.063949791357946E-5 1.3670249075326057 56-57 5.236948768340147E-4 1.2085266388477262E-4 1.5507612408587548 58-59 5.539080428052078E-4 1.2085266388477262E-4 1.739110117523173 60-61 6.042633194238631E-4 1.2085266388477262E-4 1.9278517653452165 62-63 6.042633194238631E-4 1.2085266388477262E-4 2.1057871708048967 64-65 6.042633194238631E-4 1.2085266388477262E-4 2.27941216458602 66-67 6.445475407187873E-4 1.2085266388477262E-4 2.4701881655834574 68-69 6.546185960425183E-4 1.2085266388477262E-4 2.671599201002755 70-71 6.747607066899806E-4 1.4099477453223473E-4 2.866826608453281 72-73 6.848317620137116E-4 1.4099477453223473E-4 3.055185556173023 74-75 6.949028173374426E-4 1.4099477453223473E-4 3.237300449592052 76-77 7.049738726611737E-4 1.4099477453223473E-4 3.4273815477721516 78-79 7.150449279849047E-4 1.4099477453223473E-4 3.619063943748725 80-81 7.251159833086357E-4 1.4099477453223473E-4 3.8123073533004765 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGACCG 1820 0.0 19.374556 5 TATACTG 1810 0.0 18.794014 5 TATACAG 1955 0.0 15.278122 5 GTGTAGG 1435 0.0 15.062538 1 ACTGTGC 1220 0.0 14.961567 8 ATAGACC 2485 0.0 14.523694 4 AGACCGA 2510 0.0 14.379036 6 CATAGAC 2605 0.0 13.695408 3 TATATAC 3660 0.0 13.601423 3 GTATTAA 2295 0.0 13.583925 1 TATACCG 765 0.0 13.556974 5 CTATACT 2230 0.0 13.394049 4 GGTGCGC 780 0.0 13.296265 8 GTCCTAT 1565 0.0 13.280132 1 ATACTGT 1855 0.0 13.194482 6 AGGTGCG 1070 0.0 13.181941 7 GTCCTAG 1800 0.0 13.162825 1 TACCCCG 1235 0.0 13.100319 5 TATAGTG 2630 0.0 12.934282 5 TAGACAG 1510 0.0 12.912346 5 >>END_MODULE