FastQCFastQC Report
Wed 5 Nov 2014
lane1_Undetermined_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane1_Undetermined_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4964723
Sequences flagged as poor quality0
Sequence length93
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG18200.019.3745565
TATACTG18100.018.7940145
TATACAG19550.015.2781225
GTGTAGG14350.015.0625381
ACTGTGC12200.014.9615678
ATAGACC24850.014.5236944
AGACCGA25100.014.3790366
CATAGAC26050.013.6954083
TATATAC36600.013.6014233
GTATTAA22950.013.5839251
TATACCG7650.013.5569745
CTATACT22300.013.3940494
GGTGCGC7800.013.2962658
GTCCTAT15650.013.2801321
ATACTGT18550.013.1944826
AGGTGCG10700.013.1819417
GTCCTAG18000.013.1628251
TACCCCG12350.013.1003195
TATAGTG26300.012.9342825
TAGACAG15100.012.9123465
Produced by FastQC (version 0.11.2)