##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename Raslatmar_M8_GGACTCCT-TATCCTCT_L003_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 39974195 Sequences flagged as poor quality 0 Sequence length 93 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.098528288061836 33.0 33.0 33.0 33.0 33.0 2 32.09700660638695 33.0 33.0 33.0 33.0 33.0 3 32.111273035016715 33.0 33.0 33.0 33.0 33.0 4 35.907590909585544 37.0 37.0 37.0 33.0 37.0 5 35.87320867874888 37.0 37.0 37.0 33.0 37.0 6 35.92631318779527 37.0 37.0 37.0 33.0 37.0 7 35.91033738140318 37.0 37.0 37.0 33.0 37.0 8 35.96260294922762 37.0 37.0 37.0 33.0 37.0 9 35.96544715909852 37.0 37.0 37.0 33.0 37.0 10-11 35.95438162044289 37.0 37.0 37.0 33.0 37.0 12-13 35.92784327489271 37.0 37.0 37.0 33.0 37.0 14-15 37.90932786764061 40.0 37.0 40.0 33.0 40.0 16-17 37.8521799500903 40.0 37.0 40.0 33.0 40.0 18-19 37.853112451670384 40.0 37.0 40.0 33.0 40.0 20-21 37.80953660230056 40.0 37.0 40.0 33.0 40.0 22-23 37.74569869386988 40.0 37.0 40.0 33.0 40.0 24-25 37.67916418329375 40.0 37.0 40.0 33.0 40.0 26-27 37.61082535870954 40.0 37.0 40.0 33.0 40.0 28-29 37.5099972369675 40.0 37.0 40.0 33.0 40.0 30-31 37.45275502108298 40.0 37.0 40.0 33.0 40.0 32-33 37.292075087440786 40.0 37.0 40.0 33.0 40.0 34-35 37.247210619250744 40.0 37.0 40.0 33.0 40.0 36-37 37.16361498962018 40.0 37.0 40.0 33.0 40.0 38-39 37.03486608048017 40.0 37.0 40.0 33.0 40.0 40-41 36.95685554143117 40.0 37.0 40.0 33.0 40.0 42-43 37.01905239117386 40.0 37.0 40.0 33.0 40.0 44-45 36.947468685735885 40.0 37.0 40.0 33.0 40.0 46-47 36.78162812284275 40.0 37.0 40.0 33.0 40.0 48-49 36.655040157781784 40.0 37.0 40.0 33.0 40.0 50-51 35.64324863827777 38.5 35.0 38.5 30.0 40.0 52-53 35.70875912573099 37.0 37.0 38.5 30.0 40.0 54-55 36.36503690693459 37.0 37.0 40.0 33.0 40.0 56-57 36.331866482864754 37.0 37.0 40.0 33.0 40.0 58-59 36.23739747604674 37.0 37.0 40.0 33.0 40.0 60-61 36.40756395469627 37.0 37.0 40.0 33.0 40.0 62-63 36.372879391317326 37.0 37.0 40.0 33.0 40.0 64-65 36.177955966342786 37.0 37.0 40.0 33.0 40.0 66-67 35.969346649757426 37.0 37.0 40.0 30.0 40.0 68-69 35.77329465921703 37.0 37.0 40.0 27.0 40.0 70-71 35.568174318457196 37.0 37.0 40.0 27.0 40.0 72-73 35.31390256889476 37.0 33.0 40.0 27.0 40.0 74-75 35.09062737848755 37.0 33.0 37.0 27.0 40.0 76-77 34.821737661008555 37.0 33.0 37.0 27.0 40.0 78-79 34.55965592302735 37.0 33.0 37.0 27.0 40.0 80-81 34.28691269455207 37.0 33.0 37.0 27.0 37.0 82-83 34.024632941326274 37.0 33.0 37.0 27.0 37.0 84-85 33.80795599511134 37.0 33.0 37.0 27.0 37.0 86-87 33.59381992557949 37.0 33.0 37.0 27.0 37.0 88-89 33.3995793161063 37.0 33.0 37.0 27.0 37.0 90-91 33.239052969046654 37.0 33.0 37.0 27.0 37.0 92-93 32.26055220123882 35.0 33.0 37.0 24.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2304 1 0.26430594609585967 2304 2 0.2870261628233166 2304 3 0.3133177984238813 2304 4 0.3451249505951708 2304 5 0.2977112945151461 2304 6 0.4034333218797741 2304 7 0.41660383528172673 2304 8 0.44293887563262047 2304 9 0.4346819898028258 2304 10-11 0.48979130016216743 2304 12-13 0.45097796763248255 2304 14-15 0.6513736725959376 2304 16-17 0.7064742569247642 2304 18-19 0.653878011248004 2304 20-21 0.6651543165663725 2304 22-23 0.680662260764862 2304 24-25 0.6698173811257249 2304 26-27 0.630023418681148 2304 28-29 0.6678771516504725 2304 30-31 0.5595668286426658 2304 32-33 0.7059963629188175 2304 34-35 0.6351777680536017 2304 36-37 0.6210112460131754 2304 38-39 0.6969432045903616 2304 40-41 0.7036991370769314 2304 42-43 0.5891990039460069 2304 44-45 0.6620154215959673 2304 46-47 0.687819888345814 2304 48-49 0.6972911159621731 2304 50-51 0.4978784968109977 2304 52-53 0.6860675427498748 2304 54-55 0.8682163413006023 2304 56-57 0.9067134787431499 2304 58-59 0.8862076126011047 2304 60-61 0.8397214125430565 2304 62-63 0.8110817658257972 2304 64-65 0.8526193855500921 2304 66-67 0.8358542531158832 2304 68-69 0.8346177655776614 2304 70-71 0.8066800448210074 2304 72-73 0.8422997408697697 2304 74-75 0.8253109408066095 2304 76-77 0.8050493160957117 2304 78-79 0.7932158972135852 2304 80-81 0.7140714001638955 2304 82-83 0.7302620047264767 2304 84-85 0.6796164577263824 2304 86-87 0.6547722346974538 2304 88-89 0.744110192913638 2304 90-91 0.7103650963922306 2304 92-93 0.6969238129387278 2305 1 0.30499309392498475 2305 2 0.2652789955616086 2305 3 0.3067366277614383 2305 4 0.35393359393700763 2305 5 0.3267185713560039 2305 6 0.37467575416508936 2305 7 0.4302802803512549 2305 8 0.38450888046496345 2305 9 0.46458330250396784 2305 10-11 0.45020342418261805 2305 12-13 0.49518560995009153 2305 14-15 0.7038314361175253 2305 16-17 0.7301453387381542 2305 18-19 0.7025549351724365 2305 20-21 0.7295266857852738 2305 22-23 0.7132258096070387 2305 24-25 0.7173352870909042 2305 26-27 0.6872836179790696 2305 28-29 0.7668790242788361 2305 30-31 0.744880729620867 2305 32-33 0.7681470992883845 2305 34-35 0.6977764899980983 2305 36-37 0.6698251720163242 2305 38-39 0.7606512291454024 2305 40-41 0.8365630952728864 2305 42-43 0.7353097384579499 2305 44-45 0.7311673761841959 2305 46-47 0.794939238302625 2305 48-49 0.8122536450963125 2305 50-51 0.7407005736779979 2305 52-53 0.7707244316726403 2305 54-55 0.926963040169511 2305 56-57 0.9654494853652622 2305 58-59 0.8924131034119824 2305 60-61 0.8749146661094116 2305 62-63 0.8238996088793655 2305 64-65 0.9455222655722082 2305 66-67 0.9123880392927859 2305 68-69 0.8374966466911502 2305 70-71 0.7865065842792376 2305 72-73 0.8653741847687755 2305 74-75 0.7760294102178094 2305 76-77 0.7966948271276735 2305 78-79 0.7711610133339519 2305 80-81 0.8362512901654426 2305 82-83 0.7624274982619639 2305 84-85 0.7621484139040007 2305 86-87 0.7680169860050867 2305 88-89 0.7960745706294858 2305 90-91 0.7851750758442009 2305 92-93 0.6858188750194998 2306 1 0.3281244319045271 2306 2 0.3049147535781671 2306 3 0.3306239715963102 2306 4 0.35070215008280314 2306 5 0.33521774185103936 2306 6 0.39023391065705226 2306 7 0.38846162983890764 2306 8 0.4224184819159049 2306 9 0.4795351820093927 2306 10-11 0.5335851944069816 2306 12-13 0.5224751900419733 2306 14-15 0.7866174938054087 2306 16-17 0.7790365032673776 2306 18-19 0.7438602445566147 2306 20-21 0.7428625257396106 2306 22-23 0.7531390872103998 2306 24-25 0.7644140035217291 2306 26-27 0.7852011103146666 2306 28-29 0.860604637661389 2306 30-31 0.7759796266830747 2306 32-33 0.8436342424217287 2306 34-35 0.8380554439564278 2306 36-37 0.8256719732205298 2306 38-39 0.8847285121253563 2306 40-41 0.9242671700664289 2306 42-43 0.9018816282746442 2306 44-45 0.8537292106698757 2306 46-47 0.8710164365828561 2306 48-49 0.8610650547023653 2306 50-51 0.7632286709722109 2306 52-53 0.8886990558175825 2306 54-55 1.0154627805464216 2306 56-57 1.0613651990065236 2306 58-59 1.0197191566807433 2306 60-61 0.9756297408200965 2306 62-63 0.9277763275013413 2306 64-65 0.9727195091483765 2306 66-67 0.9849278233084817 2306 68-69 0.9986043405568665 2306 70-71 0.9319416619359941 2306 72-73 0.9520753650411038 2306 74-75 0.9614241882953181 2306 76-77 0.9088756380355321 2306 78-79 0.9176234232254075 2306 80-81 0.8752771935525061 2306 82-83 0.9056519258482965 2306 84-85 0.8721177139343865 2306 86-87 0.8644427531360677 2306 88-89 0.929809206374614 2306 90-91 0.959378370847837 2306 92-93 0.9301067316560037 2307 1 0.30140156345401437 2307 2 0.3037542668314117 2307 3 0.32331366573517073 2307 4 0.32655444616614204 2307 5 0.4108206589318897 2307 6 0.4132655174165194 2307 7 0.3683447101048287 2307 8 0.3480012107574666 2307 9 0.40919047794861285 2307 10-11 0.4290678522985161 2307 12-13 0.4311314766948655 2307 14-15 0.5823781276256597 2307 16-17 0.4717247931869295 2307 18-19 0.5566275255076434 2307 20-21 0.525834329754943 2307 22-23 0.5730436442715927 2307 24-25 0.6015464631596643 2307 26-27 0.539393212320114 2307 28-29 0.5708590792440162 2307 30-31 0.49703367914914054 2307 32-33 0.6268369119165413 2307 34-35 0.532431058209994 2307 36-37 0.5413901393840135 2307 38-39 0.5807808052524521 2307 40-41 0.5729874078229358 2307 42-43 0.48791350146625234 2307 44-45 0.499817257807301 2307 46-47 0.48193846028459575 2307 48-49 0.43821672332138917 2307 50-51 0.4284877908223308 2307 52-53 0.4727699101372238 2307 54-55 0.5939397232653647 2307 56-57 0.686621560304765 2307 58-59 0.6825927532928873 2307 60-61 0.6535904119630231 2307 62-63 0.5776136113716603 2307 64-65 0.6714690156391967 2307 66-67 0.6695154166613406 2307 68-69 0.6555981353856453 2307 70-71 0.6051653692227887 2307 72-73 0.6678152648241849 2307 74-75 0.6157947625101556 2307 76-77 0.5974830866799081 2307 78-79 0.5150466309057435 2307 80-81 0.5393606477841999 2307 82-83 0.6205706448307637 2307 84-85 0.519637286903901 2307 86-87 0.5557732110224194 2307 88-89 0.4354116911037309 2307 90-91 0.6149193540969762 2307 92-93 0.6459177130205376 2308 1 0.277519730769356 2308 2 0.2458902658657749 2308 3 0.24980111737038868 2308 4 0.33196305093114375 2308 5 0.3370363713753335 2308 6 0.3273100816869743 2308 7 0.32412608449264724 2308 8 0.33741537051309933 2308 9 0.37758534851806047 2308 10-11 0.41951084889542756 2308 12-13 0.43668509062293737 2308 14-15 0.5704031278752666 2308 16-17 0.6485566325850627 2308 18-19 0.6086016480909748 2308 20-21 0.6146320485768229 2308 22-23 0.5996493499780939 2308 24-25 0.5730313471615887 2308 26-27 0.5597967956740604 2308 28-29 0.5495002257894441 2308 30-31 0.5524236554635493 2308 32-33 0.6168511898438851 2308 34-35 0.585293206177198 2308 36-37 0.6082755926570727 2308 38-39 0.6541691305166708 2308 40-41 0.6697048107480299 2308 42-43 0.6334784074865851 2308 44-45 0.6535212352694089 2308 46-47 0.6874700402193099 2308 48-49 0.616642086229632 2308 50-51 0.5360019177696529 2308 52-53 0.6364252596630777 2308 54-55 0.7656543919127685 2308 56-57 0.7794713537501963 2308 58-59 0.7666137773569943 2308 60-61 0.6847773452830239 2308 62-63 0.6363822902131204 2308 64-65 0.7142864067394754 2308 66-67 0.7320424153014784 2308 68-69 0.7285361668044672 2308 70-71 0.7250837242126664 2308 72-73 0.7070211464164373 2308 74-75 0.6644717246962983 2308 76-77 0.6242827334924002 2308 78-79 0.603532402539436 2308 80-81 0.6392658029012992 2308 82-83 0.6285092607373315 2308 84-85 0.6428404490519171 2308 86-87 0.5847482442758647 2308 88-89 0.6479121160223968 2308 90-91 0.6769224603978969 2308 92-93 0.710341576936635 2309 1 0.24925485169615058 2309 2 0.20433393593627613 2309 3 0.1893171074139346 2309 4 0.2300123877481397 2309 5 0.3399283980999144 2309 6 0.3400841328458952 2309 7 0.3324906107087955 2309 8 0.26030640796832216 2309 9 0.329418508213962 2309 10-11 0.29349732941160767 2309 12-13 0.3344199314637919 2309 14-15 0.45213434260924146 2309 16-17 0.46994437701318503 2309 18-19 0.43776419693865876 2309 20-21 0.4081945016694206 2309 22-23 0.37900636586230974 2309 24-25 0.4188885770203683 2309 26-27 0.40644167536979836 2309 28-29 0.42227645249204215 2309 30-31 0.33638475841281235 2309 32-33 0.43016645735556125 2309 34-35 0.40778879350634156 2309 36-37 0.4520026630344489 2309 38-39 0.3800928345718191 2309 40-41 0.3303456753770888 2309 42-43 0.3442980539238292 2309 44-45 0.3555727900489316 2309 46-47 0.4556383145928464 2309 48-49 0.44773888768035164 2309 50-51 0.33451460858955073 2309 52-53 0.40161268035272 2309 54-55 0.49978672992458684 2309 56-57 0.43894211585467957 2309 58-59 0.3486428360366105 2309 60-61 0.3879639275370934 2309 62-63 0.35671570569908084 2309 64-65 0.4608280581131794 2309 66-67 0.5471757044333572 2309 68-69 0.4527980467843662 2309 70-71 0.504314415804302 2309 72-73 0.5031283230423469 2309 74-75 0.4489565416686361 2309 76-77 0.4185289924177624 2309 78-79 0.38764570904417894 2309 80-81 0.3288741015196379 2309 82-83 0.29820875656358226 2309 84-85 0.3202160693124654 2309 86-87 0.3483533956716016 2309 88-89 0.3813084616093576 2309 90-91 0.40523824886436444 2309 92-93 0.46817127482202636 2310 1 0.1540100411941836 2310 2 0.01702646438901212 2310 3 0.05499958501080471 2310 4 0.10921294842786722 2310 5 0.15089025663173317 2310 6 0.17481846623621777 2310 7 0.18429241118460737 2310 8 0.08676905626936815 2310 9 0.1391981521480119 2310 10-11 0.10934895951579193 2310 12-13 0.12623023297786773 2310 14-15 0.1595334394574479 2310 16-17 0.14842005388423019 2310 18-19 0.12669192836847287 2310 20-21 0.08613918786478081 2310 22-23 0.07982482274531577 2310 24-25 0.1215170534073593 2310 26-27 0.01851150242496402 2310 28-29 0.10319449304181205 2310 30-31 0.05484834785602999 2310 32-33 0.12129781753766622 2310 34-35 -0.03355485690728699 2310 36-37 0.005276714292740792 2310 38-39 0.02768315382629538 2310 40-41 0.020589129242964077 2310 42-43 -0.046214164383130196 2310 44-45 -0.05069980199214541 2310 46-47 0.11554338682222465 2310 48-49 -0.05035446081580375 2310 50-51 -0.12786413554754006 2310 52-53 -0.04820363408153838 2310 54-55 0.02617466510075417 2310 56-57 0.105994971640051 2310 58-59 0.09954323327787762 2310 60-61 0.08502475225716921 2310 62-63 0.06939725296663113 2310 64-65 0.11229997738081465 2310 66-67 0.0192521536460859 2310 68-69 0.05330248687638317 2310 70-71 0.07493661337531421 2310 72-73 0.04799572427762655 2310 74-75 0.06041404117582516 2310 76-77 0.0347812603246922 2310 78-79 -0.04653627703583396 2310 80-81 -0.04488124050622133 2310 82-83 -0.05832438379008664 2310 84-85 -0.049905020213088847 2310 86-87 -0.05370956365205615 2310 88-89 -0.02248588005964791 2310 90-91 -0.049441922141177486 2310 92-93 -0.03650489415802838 2311 1 0.24777754007165953 2311 2 0.20956349013786735 2311 3 0.16827998723593396 2311 4 0.23083490802220297 2311 5 0.25672118797545096 2311 6 0.30725177975839557 2311 7 0.2730590967190949 2311 8 0.2657795242801555 2311 9 0.29482629529267257 2311 10-11 0.2817594659790572 2311 12-13 0.27967225969236864 2311 14-15 0.3101232733948649 2311 16-17 0.33160257027650886 2311 18-19 0.2380850267041481 2311 20-21 0.23607677609836486 2311 22-23 0.2827273595727746 2311 24-25 0.31117737727129224 2311 26-27 0.2524530351113796 2311 28-29 0.2603129170948648 2311 30-31 0.2577768793503381 2311 32-33 0.279097587054963 2311 34-35 0.2201490550039935 2311 36-37 0.24805533324381912 2311 38-39 0.25851024787396426 2311 40-41 0.28672734726004023 2311 42-43 0.25095864717945204 2311 44-45 0.11089739277165478 2311 46-47 0.2072588840174987 2311 48-49 0.266992295099179 2311 50-51 0.17266119806078706 2311 52-53 0.2695909531617602 2311 54-55 0.38028094705017423 2311 56-57 0.4126815207834511 2311 58-59 0.3214018012744688 2311 60-61 0.2928349921563296 2311 62-63 0.24993650677468082 2311 64-65 0.34267642115470665 2311 66-67 0.38485208760147316 2311 68-69 0.32908296709847207 2311 70-71 0.3200275987535832 2311 72-73 0.2576221591263277 2311 74-75 0.2582771758376907 2311 76-77 0.1681325999555412 2311 78-79 0.21836217704692729 2311 80-81 0.22033090900287533 2311 82-83 0.22493870703915775 2311 84-85 0.1973846939481092 2311 86-87 0.15266639142819116 2311 88-89 0.14641368612836914 2311 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88-89 -0.24481143002533656 2303 90-91 -0.23409171302913734 2303 92-93 -0.5502805403450601 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 99031.0 3 2942.0 4 1903.0 5 1534.0 6 1433.0 7 2650.0 8 5923.0 9 8860.0 10 12426.0 11 19639.0 12 31460.0 13 43692.0 14 53338.0 15 61467.0 16 69686.0 17 79333.0 18 88942.0 19 99733.0 20 113071.0 21 127940.0 22 145097.0 23 165836.0 24 190463.0 25 222626.0 26 261384.0 27 310240.0 28 372609.0 29 450661.0 30 562536.0 31 726920.0 32 959123.0 33 1306073.0 34 1861735.0 35 2827949.0 36 4830343.0 37 9792072.0 38 1.3428641E7 39 634884.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.54304926744347 23.869493041128052 10.556580902021395 24.03087678940708 2 15.874767021848452 22.328950078622274 38.62272862001577 23.173554279513496 3 15.593452399582597 27.090459599955608 31.66276905819515 25.653318942266644 4 11.57065526913083 17.052000214735017 42.23466251746763 29.142681998666525 5 11.72931455511628 37.05838526268892 38.14112573586179 13.071174446333004 6 28.1079507363804 40.905809841794024 18.989469411773385 11.996770010052192 7 25.870164876830813 32.46654512661208 26.12011372504513 15.543176271511971 8 23.077993214359836 36.37720466067183 23.658118482158592 16.886683642809746 9 22.65642746956224 15.454115489475345 26.626768441167766 35.26268859979466 10-11 22.16395423168694 27.041803999966536 31.784702779123375 19.00953898922315 12-13 21.477870827642295 26.03116816000664 31.930101346808566 20.560859665542498 14-15 19.48641089656797 27.374862210566935 27.751846624231636 25.386880268633455 16-17 19.766109876676243 31.6898501962621 28.701023269916625 19.84301665714503 18-19 19.83704457436029 30.487771031407107 30.215557435129902 19.459626959102692 20-21 20.102711131017593 30.079929538720762 29.991397656500734 19.82596167376091 22-23 20.213006550307842 29.84486111684623 29.932874308878127 20.009258023967806 24-25 19.804072227970355 29.734400332404864 30.242137270550256 20.219390169074526 26-27 20.037459995184545 30.271440471374618 29.932666682152913 19.758432851287925 28-29 20.06457740905929 30.11021846279468 29.90719276979446 19.918011358351567 30-31 19.848070769232002 29.94357858902223 30.123243672746785 20.08510696899898 32-33 20.072413581183707 30.22071367562639 29.965505218177647 19.74136752501226 34-35 19.913891036181166 29.93001506539984 30.095369462280246 20.060724436138745 36-37 19.871487491752884 30.125661669307803 30.139459328424124 19.863391510515193 38-39 19.932471231279994 30.108037952198057 30.14437906480983 19.81511175171212 40-41 19.8364317366893 29.985379716465687 30.27135462981415 19.906833917030863 42-43 19.83196994588232 30.027678671227097 30.281684036973015 19.858667345917564 44-45 19.754336964230347 29.883390984661794 30.304923322137988 20.057348728969874 46-47 19.744018245341262 29.88801897216894 30.427018032931798 19.940944749558 48-49 19.734585129054594 30.10295109758593 30.318934894286464 19.843528879073013 50-51 19.576332305731693 30.036453897644392 30.432126782260116 19.955087014363794 52-53 19.658216063087895 29.868368429158497 30.555490279906973 19.917925227846638 54-55 19.636570650264424 30.01315930282035 30.447084165102716 19.90318588181251 56-57 19.640651768360907 30.002433094693387 30.379658721312474 19.977256415633228 58-59 19.688214541059267 29.997763363956803 30.312506671213523 20.001515423770407 60-61 19.533195311180545 29.949911525933544 30.460419884783512 20.056473278102395 62-63 19.526914193884963 30.006528675289275 30.38000743493053 20.086549695895236 64-65 19.678894065445366 29.8896044700563 30.38677130044843 20.044730164049902 66-67 19.638267545399994 29.819706979628453 30.340059713863127 20.201965761108422 68-69 19.6416020848214 29.987513729215898 30.234118455209437 20.136765730753265 70-71 19.766911353168894 29.955836117883106 30.178313702864727 20.09893882608327 72-73 19.765466707228413 29.971432113223457 30.090636587965243 20.17246459158289 74-75 19.736197212658976 30.065461494026007 30.144657038667077 20.05368425464794 76-77 19.802089853956556 30.05086401179775 30.07382082731538 20.07322530693032 78-79 19.947753638317632 30.0256452703686 30.019482971595902 20.007118119717866 80-81 19.89084527138325 30.114969236157307 29.977334769948722 20.01685072251072 82-83 20.00197768814577 30.10447230443915 29.99108761007977 19.902462397335306 84-85 19.988749606807552 30.143592208227872 29.993804369411496 19.873853815553076 86-87 19.88751810755735 30.03221150364474 30.07945062745231 20.000819761345596 88-89 20.028119182366265 29.99511027457193 29.983795321492046 19.992975221569758 90-91 20.182411538448466 30.086820238505595 29.90607732388258 19.824690899163357 92-93 20.342711113998856 29.944856878413006 29.848215612919958 19.864216394668173 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 94751.0 1 48869.0 2 2506.0 3 1893.0 4 1734.5 5 1705.0 6 1753.5 7 1949.0 8 2401.5 9 3048.0 10 3944.0 11 5324.5 12 7508.0 13 10773.5 14 12678.0 15 15541.5 16 22955.5 17 34869.0 18 53057.0 19 79765.0 20 117813.5 21 169432.5 22 237759.0 23 324755.5 24 428801.5 25 552029.5 26 695648.0 27 854208.0 28 1022487.0 29 1108519.0 30 1185254.5 31 1333375.0 32 1467594.0 33 1584626.0 34 1683033.0 35 1757985.0 36 1801455.0 37 1811709.5 38 1793229.0 39 1754332.0 40 1703260.0 41 1637682.5 42 1565289.0 43 1530290.0 44 1485143.5 45 1382081.0 46 1269731.5 47 1159910.0 48 1050595.5 49 943980.0 50 843072.0 51 750788.5 52 665467.0 53 587443.0 54 516492.5 55 452841.0 56 399128.5 57 374884.0 58 354555.0 59 315243.5 60 277028.0 61 242222.0 62 211671.0 63 184250.0 64 160398.5 65 140037.0 66 122887.5 67 107291.0 68 91776.0 69 76247.5 70 61967.0 71 55100.0 72 50451.0 73 42234.0 74 35486.0 75 30045.5 76 26463.0 77 23363.0 78 19310.0 79 15733.5 80 12530.5 81 9780.5 82 7923.0 83 6133.5 84 4630.5 85 3162.5 86 2417.0 87 2009.5 88 1360.5 89 908.0 90 563.0 91 345.0 92 216.0 93 152.0 94 131.0 95 108.0 96 70.5 97 45.5 98 32.5 99 20.5 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4043508568465231 2 0.40925151838579865 3 0.41104767713271023 4 0.41775450387431196 5 0.4227627348092938 6 0.3006964868210604 7 0.2812789600891275 8 0.2899545569335418 9 0.28169672960268494 10-11 0.28189810951790273 12-13 0.2733000627029512 14-15 0.2727234407096878 16-17 0.2679203421107042 18-19 0.2662254987248649 20-21 0.2724144913987636 22-23 0.2815741505238567 24-25 0.27406555654216425 26-27 0.2773514263389169 28-29 0.27155268542618555 30-31 0.2731437118370989 32-33 0.2720379985137912 34-35 0.27850467032544374 36-37 0.2720179856029621 38-39 0.27925265286768125 40-41 0.27788677170359527 42-43 0.28177052721136725 44-45 0.2852502720817768 46-47 0.2696714718082503 48-49 0.26151245822461217 50-51 0.25735477599986695 52-53 0.25154727943864785 54-55 0.2761306387783419 56-57 0.2734926869696813 58-59 0.2770737472011632 60-61 0.2850013615033399 62-63 0.2810725769462024 64-65 0.27696617780545674 66-67 0.27706624235960225 68-69 0.2782094798907145 70-71 0.2778742636343271 72-73 0.2686808327222099 74-75 0.2709648061705808 76-77 0.25392506340653015 78-79 0.25694200971401676 80-81 0.2604042432874508 82-83 0.27384416371611736 84-85 0.2747034680748418 86-87 0.27251080353212864 88-89 0.2777191635754016 90-91 0.27269717376422464 92-93 0.26162002762031855 >>END_MODULE >>Sequence Length Distribution pass #Length Count 93 3.9974195E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.04826966553797 #Duplication Level Percentage of deduplicated Percentage of total 1 89.36953373973284 67.07028867982865 2 6.77059542755913 10.162429428874319 3 1.4770672303069068 3.325540194426061 4 0.7012623525630554 2.10514104565767 5 0.371830818362904 1.3952629763228444 6 0.22688381584305076 1.0216342676481316 7 0.1660291964149297 0.872214273343015 8 0.14321454197157912 0.8598402852727662 9 0.08856666465412229 0.5982097439105856 >10 0.6336715949679367 8.6485662061322 >50 0.03840022289041614 1.9056598709171442 >100 0.012652911521346576 1.5098944149820217 >500 2.4211742964744864E-4 0.12002442217125997 >1k 2.5367520095743975E-5 0.029506625383172846 >5k 1.70843716289137E-5 0.10126918357498847 >10k+ 6.913890395183393E-6 0.27451838155512964 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 95738 0.2394995071195305 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 5.003227707274656E-6 0.0 2 0.0 5.003227707274656E-6 5.003227707274656E-6 3 0.0 7.504841560911983E-6 5.003227707274656E-6 4 2.501613853637328E-6 7.504841560911983E-6 7.504841560911983E-6 5 5.003227707274656E-6 7.504841560911983E-6 7.504841560911983E-6 6 1.0006455414549312E-5 7.504841560911983E-6 7.504841560911983E-6 7 1.0006455414549312E-5 7.504841560911983E-6 1.0006455414549312E-5 8 2.0012910829098624E-5 7.504841560911983E-6 1.5009683121823966E-5 9 2.7517752390010606E-5 1.0006455414549312E-5 2.251452468273595E-5 10-11 3.12701731704666E-5 1.8762103902279956E-5 6.754357404820785E-5 12-13 4.878147014592789E-5 3.0019366243647932E-5 8.255325717003182E-5 14-15 5.8787925560477204E-5 3.627340087774125E-5 9.631213336503712E-5 16-17 6.504196019457052E-5 4.50290493654719E-5 1.2633149960868505E-4 18-19 7.62992225359385E-5 4.50290493654719E-5 2.0513233599826089E-4 20-21 9.255971258458114E-5 5.1283083999565214E-5 2.9393962780238604E-4 22-23 1.000645541454931E-4 5.503550478002121E-5 4.703034044838176E-4 24-25 1.0882020263322376E-4 6.003873248729587E-5 0.0011407359172586214 26-27 1.275823065355037E-4 6.754357404820785E-5 0.006411636306872471 28-29 1.3633795502323437E-4 7.62992225359385E-5 0.03459982120965788 30-31 1.45093603510965E-4 8.255325717003182E-5 0.060013716348759495 32-33 1.4759521736460235E-4 9.130890565776246E-5 0.08395165931421508 34-35 1.663573212668823E-4 9.756294029185578E-5 0.1256910864621539 36-37 1.801161974618876E-4 1.038169749259491E-4 0.21635457574567793 38-39 1.876210390227996E-4 1.0882020263322376E-4 0.3724677882819154 40-41 2.001291082909862E-4 1.1132181648686109E-4 0.5517947265729803 42-43 2.0888475677871687E-4 1.1507423726731707E-4 0.7307151526128294 44-45 2.2639605375417816E-4 1.2257907882822907E-4 0.93793133295117 46-47 2.3640250916872748E-4 1.2382988575504773E-4 1.1849544437355148 48-49 2.5766622692464475E-4 1.275823065355037E-4 1.4373998025476185 50-51 2.7267591004646874E-4 1.3383634116959705E-4 1.688412987428515 52-53 2.9393962780238604E-4 1.4259198965732768E-4 1.9269256078827852 54-55 3.1270173170466596E-4 1.52598445071877E-4 2.1710581038592522 56-57 3.302130286801273E-4 1.60103286632789E-4 2.4412273968243765 58-59 3.4772432565558853E-4 1.6260490048642631E-4 2.720271164935279 60-61 4.090138650697031E-4 1.663573212668823E-4 2.9960503269671848 62-63 4.215219343378897E-4 1.7010974204733828E-4 3.257272597984775 64-65 4.3903323131335104E-4 1.7511296975461293E-4 3.5109712653375507 66-67 4.4778887980108167E-4 1.7761458360825028E-4 3.7806602484427767 68-69 4.5779533521563096E-4 1.8261781131552492E-4 4.0634964631557935 70-71 4.778082460447296E-4 1.863702320959809E-4 4.344421444884631 72-73 4.940687360933723E-4 1.9012265287643692E-4 4.613732684297958 74-75 5.140816469224709E-4 1.9637668751053023E-4 4.870644674645731 76-77 5.228372954102015E-4 2.0513233599826089E-4 5.133861982711597 78-79 5.328437508247509E-4 2.1013556370553553E-4 5.410080177974816 80-81 5.378469785320255E-4 2.2014201912008485E-4 5.688442256310602 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACTG 17255 0.0 15.069492 5 GTATAAG 19100 0.0 13.2498 1 GTATAGG 13910 0.0 12.650882 1 GTATAGA 21595 0.0 12.5257845 1 GTATTAG 18325 0.0 12.170054 1 GTGTAAG 12595 0.0 11.965875 1 GTATATA 35145 0.0 11.8608265 1 CTATACT 19650 0.0 11.748107 4 TTATACT 30160 0.0 11.712352 4 TATACAG 16455 0.0 11.567056 5 GTGTAGG 14840 0.0 11.182982 1 ACTGTGC 11530 0.0 11.130191 8 GTATAAT 27570 0.0 11.122501 1 GTACTAG 13255 0.0 10.712835 1 GTATTAT 29875 0.0 10.585107 1 CTTACAC 12280 0.0 10.570071 3 TATACCG 6100 0.0 10.567473 5 GTCTTAG 14635 0.0 10.565796 1 GTCCTAG 14595 0.0 10.564908 1 TAGACAG 12025 0.0 10.540159 5 >>END_MODULE