FastQCFastQC Report
Wed 5 Nov 2014
Raslatmar_M8_GGACTCCT-TATCCTCT_L003_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRaslatmar_M8_GGACTCCT-TATCCTCT_L003_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39974195
Sequences flagged as poor quality0
Sequence length93
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN957380.2394995071195305No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG172550.015.0694925
GTATAAG191000.013.24981
GTATAGG139100.012.6508821
GTATAGA215950.012.52578451
GTATTAG183250.012.1700541
GTGTAAG125950.011.9658751
GTATATA351450.011.86082651
CTATACT196500.011.7481074
TTATACT301600.011.7123524
TATACAG164550.011.5670565
GTGTAGG148400.011.1829821
ACTGTGC115300.011.1301918
GTATAAT275700.011.1225011
GTACTAG132550.010.7128351
GTATTAT298750.010.5851071
CTTACAC122800.010.5700713
TATACCG61000.010.5674735
GTCTTAG146350.010.5657961
GTCCTAG145950.010.5649081
TAGACAG120250.010.5401595
Produced by FastQC (version 0.11.2)