##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename Raslatmar_M8_GGACTCCT-TATCCTCT_L003_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 39974195 Sequences flagged as poor quality 0 Sequence length 93 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.46403913824906 33.0 33.0 33.0 33.0 33.0 2 32.52819079908926 33.0 33.0 33.0 33.0 33.0 3 32.60263204799997 33.0 33.0 33.0 33.0 33.0 4 36.601965242827276 37.0 37.0 37.0 37.0 37.0 5 36.45936189584305 37.0 37.0 37.0 37.0 37.0 6 36.46203489526181 37.0 37.0 37.0 37.0 37.0 7 36.4323218266184 37.0 37.0 37.0 37.0 37.0 8 36.41488570313924 37.0 37.0 37.0 37.0 37.0 9 36.47115695513068 37.0 37.0 37.0 37.0 37.0 10-11 36.43321635370018 37.0 37.0 37.0 37.0 37.0 12-13 36.44242275548013 37.0 37.0 37.0 37.0 37.0 14-15 38.489561228187334 40.0 37.0 40.0 37.0 40.0 16-17 38.45640182372653 40.0 37.0 40.0 37.0 40.0 18-19 38.42983319614066 40.0 37.0 40.0 37.0 40.0 20-21 38.392070684600405 40.0 37.0 40.0 37.0 40.0 22-23 38.31633368727 40.0 37.0 40.0 37.0 40.0 24-25 38.294736229209875 40.0 37.0 40.0 37.0 40.0 26-27 38.26477972852236 40.0 37.0 40.0 37.0 40.0 28-29 38.18153522541229 40.0 37.0 40.0 37.0 40.0 30-31 38.16143005506427 40.0 37.0 40.0 37.0 40.0 32-33 38.09418343508856 40.0 37.0 40.0 35.0 40.0 34-35 38.03743964074824 40.0 37.0 40.0 33.0 40.0 36-37 37.962082926247795 40.0 37.0 40.0 33.0 40.0 38-39 37.85998559820904 40.0 37.0 40.0 33.0 40.0 40-41 37.77450784187149 40.0 37.0 40.0 33.0 40.0 42-43 37.70940840709863 40.0 37.0 40.0 33.0 40.0 44-45 37.62209905665392 40.0 37.0 40.0 33.0 40.0 46-47 37.5198590615771 40.0 37.0 40.0 33.0 40.0 48-49 37.393772407424336 40.0 37.0 40.0 33.0 40.0 50-51 37.24128108145767 40.0 37.0 40.0 33.0 40.0 52-53 37.22789876068799 40.0 37.0 40.0 33.0 40.0 54-55 37.06478441904834 38.5 37.0 40.0 33.0 40.0 56-57 36.94785031943733 37.0 37.0 40.0 33.0 40.0 58-59 36.75971403551716 37.0 37.0 40.0 33.0 40.0 60-61 36.56005860780937 37.0 37.0 40.0 33.0 40.0 62-63 36.372013507714165 37.0 37.0 40.0 33.0 40.0 64-65 36.17262806668152 37.0 37.0 40.0 33.0 40.0 66-67 35.964625716665466 37.0 37.0 40.0 33.0 40.0 68-69 35.716762801602385 37.0 33.0 40.0 33.0 40.0 70-71 35.525202196066736 37.0 33.0 38.5 33.0 40.0 72-73 35.25719694918184 37.0 33.0 37.0 27.0 40.0 74-75 34.96802683581245 37.0 33.0 37.0 27.0 40.0 76-77 33.42379467554006 35.0 33.0 37.0 27.0 37.0 78-79 34.47711217198996 37.0 33.0 37.0 27.0 37.0 80-81 34.538437046699755 37.0 33.0 37.0 27.0 37.0 82-83 34.3973564445763 37.0 33.0 37.0 27.0 37.0 84-85 34.25125013774511 37.0 33.0 37.0 27.0 37.0 86-87 34.06841442335487 37.0 33.0 37.0 27.0 37.0 88-89 33.83642524883866 37.0 33.0 37.0 27.0 37.0 90-91 33.7118297441637 37.0 33.0 37.0 27.0 37.0 92-93 32.67125713225745 35.0 33.0 37.0 24.5 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2304 1 0.47366175417190703 2304 2 0.35949301962030944 2304 3 0.30994120568908556 2304 4 0.2640449050292588 2304 5 0.1603283301093441 2304 6 0.11194367638601932 2304 7 0.12154869637547705 2304 8 0.13577259192264535 2304 9 0.12669983519509742 2304 10-11 0.10118395512934342 2304 12-13 0.09247418967816401 2304 14-15 0.23234863278708673 2304 16-17 0.21286139208365285 2304 18-19 0.21299016104858026 2304 20-21 0.2595074900582688 2304 22-23 0.2273933793496994 2304 24-25 0.2393571127109766 2304 26-27 0.24004091025949492 2304 28-29 0.13378440569549355 2304 30-31 0.11277803482688853 2304 32-33 0.16226309824952523 2304 34-35 0.16374519914734265 2304 36-37 0.12651263060458717 2304 38-39 0.13959804822708577 2304 40-41 0.16304016923050568 2304 42-43 0.16372357260385684 2304 44-45 0.18587454145845328 2304 46-47 0.19758052381249058 2304 48-49 0.15280425410590226 2304 50-51 0.17526182655646494 2304 52-53 0.18072853862716443 2304 54-55 0.13991178165458962 2304 56-57 0.15312062787309344 2304 58-59 0.26452678834147036 2304 60-61 0.14974619229349884 2304 62-63 0.12456685402373324 2304 64-65 0.11571218116669968 2304 66-67 0.023913404557859508 2304 68-69 -0.01726684776809151 2304 70-71 -0.0146706321487855 2304 72-73 0.05699453016800504 2304 74-75 -0.05741104049382528 2304 76-77 0.051263024948013936 2304 78-79 0.19784289062314286 2304 80-81 0.29523146219641916 2304 82-83 0.2540950334461911 2304 84-85 0.27260552473902067 2304 86-87 0.20627975025066547 2304 88-89 0.3152107196641367 2304 90-91 0.2778391677252401 2304 92-93 0.2074763712381511 2305 1 0.5718305626992759 2305 2 0.43391679120488647 2305 3 0.36081029922510055 2305 4 0.35648258918653397 2305 5 0.3094261521441908 2305 6 0.24676365172521741 2305 7 0.25275609839885504 2305 8 0.27131239360604553 2305 9 0.2515253902306185 2305 10-11 0.25218068856533193 2305 12-13 0.2615265533930966 2305 14-15 0.459979238500587 2305 16-17 0.4749580194193328 2305 18-19 0.42998888143591785 2305 20-21 0.49184733909291367 2305 22-23 0.5189457881521164 2305 24-25 0.4959514385777908 2305 26-27 0.4716566918373388 2305 28-29 0.43729347650113226 2305 30-31 0.3849139864559561 2305 32-33 0.44866349502342473 2305 34-35 0.506081244613128 2305 36-37 0.4590309809564559 2305 38-39 0.5144638890167883 2305 40-41 0.47992071682195814 2305 42-43 0.3760940398659969 2305 44-45 0.479577963288655 2305 46-47 0.43537805975800836 2305 48-49 0.41180631641089604 2305 50-51 0.458881168114182 2305 52-53 0.530004422509343 2305 54-55 0.5411060910232806 2305 56-57 0.5337781597905007 2305 58-59 0.38850276443325527 2305 60-61 0.36794652424482877 2305 62-63 0.39406431102383266 2305 64-65 0.35458457531930776 2305 66-67 0.3351659131224807 2305 68-69 0.3980777718009705 2305 70-71 0.3313056002446757 2305 72-73 0.4262900763489412 2305 74-75 0.4086438561998804 2305 76-77 0.40348418537988096 2305 78-79 0.5376098853066864 2305 80-81 0.583238660763989 2305 82-83 0.5287902879387474 2305 84-85 0.4373484973492836 2305 86-87 0.5525659108043399 2305 88-89 0.617919579151156 2305 90-91 0.5589952272968404 2305 92-93 0.554925938874284 2306 1 0.5976136227532294 2306 2 0.44790068720834597 2306 3 0.3391595162129164 2306 4 0.3400946994066558 2306 5 0.3384359460108257 2306 6 0.3153063541990022 2306 7 0.2800182035554144 2306 8 0.2584048083769446 2306 9 0.19135664995497592 2306 10-11 0.22169546774760818 2306 12-13 0.20636662096875114 2306 14-15 0.43250871953060255 2306 16-17 0.43181073027102457 2306 18-19 0.4437429060540836 2306 20-21 0.41753331760496337 2306 22-23 0.46559926038073485 2306 24-25 0.4806801177223221 2306 26-27 0.46564793738369303 2306 28-29 0.46400838825690016 2306 30-31 0.45734024521363636 2306 32-33 0.4295911191039252 2306 34-35 0.3954744087925093 2306 36-37 0.42433987166618437 2306 38-39 0.4736978729422532 2306 40-41 0.48226156971141876 2306 42-43 0.4592442180116265 2306 44-45 0.3934002116982285 2306 46-47 0.43093218564759184 2306 48-49 0.3265964719085517 2306 50-51 0.45682931346628664 2306 52-53 0.4089377905204685 2306 54-55 0.4554345934031616 2306 56-57 0.334159125367421 2306 58-59 0.4689114194567807 2306 60-61 0.4248041126962008 2306 62-63 0.3908941198049902 2306 64-65 0.2718223274621181 2306 66-67 0.3427208316910537 2306 68-69 0.35558916023208553 2306 70-71 0.38085838198635713 2306 72-73 0.3427011572832228 2306 74-75 0.2565883797178614 2306 76-77 0.2283205768031138 2306 78-79 0.46640610327960275 2306 80-81 0.555558035827282 2306 82-83 0.489760470871758 2306 84-85 0.4356687958237657 2306 86-87 0.5763097014855276 2306 88-89 0.6573835145584042 2306 90-91 0.5960678020765684 2306 92-93 0.5943171788967518 2307 1 0.5742421091895764 2307 2 0.4085026035981727 2307 3 0.3370412617314287 2307 4 0.3274803660228258 2307 5 0.3498560196953875 2307 6 0.285393694422865 2307 7 0.18000234991941255 2307 8 0.2444093351365595 2307 9 0.20234683446200563 2307 10-11 0.20662458571311504 2307 12-13 0.2042666512703235 2307 14-15 0.3834030893502387 2307 16-17 0.3423527434041773 2307 18-19 0.3551078560010552 2307 20-21 0.37079763565324697 2307 22-23 0.3617441444033034 2307 24-25 0.36458207808638576 2307 26-27 0.3016241924047023 2307 28-29 0.3375894098633978 2307 30-31 0.23156335866347177 2307 32-33 0.2962056010393752 2307 34-35 0.36851836935611715 2307 36-37 0.3452650570785636 2307 38-39 0.3529812602887574 2307 40-41 0.42992646496774256 2307 42-43 0.4161999873205602 2307 44-45 0.3447986382335344 2307 46-47 0.3520463331571335 2307 48-49 0.3305864084600074 2307 50-51 0.311780445895252 2307 52-53 0.403429317165525 2307 54-55 0.4039034577962255 2307 56-57 0.3492228041263843 2307 58-59 0.39494572712122533 2307 60-61 0.3069345764950526 2307 62-63 0.3840099752886488 2307 64-65 0.3908015716226032 2307 66-67 0.3662711493032873 2307 68-69 0.37735161621166213 2307 70-71 0.3665968823540382 2307 72-73 0.39070694266677464 2307 74-75 0.3841484104628776 2307 76-77 0.33902550228710737 2307 78-79 0.4877678530846765 2307 80-81 0.5150350698204775 2307 82-83 0.41714763224241835 2307 84-85 0.3552766213901677 2307 86-87 0.37524359010136976 2307 88-89 0.5740236375384598 2307 90-91 0.48017879452704193 2307 92-93 0.44496252224684696 2308 1 0.5589435967438448 2308 2 0.38305076863959187 2308 3 0.3329950105127324 2308 4 0.3050751850771931 2308 5 0.31147129446362953 2308 6 0.23996850447003482 2308 7 0.21202941919021612 2308 8 0.22583787675488054 2308 9 0.1880201663263037 2308 10-11 0.2176144302892311 2308 12-13 0.1691307304512364 2308 14-15 0.3108855468720577 2308 16-17 0.3316721124144948 2308 18-19 0.33820143931936286 2308 20-21 0.3008615633085441 2308 22-23 0.31707636240071935 2308 24-25 0.3234182396990377 2308 26-27 0.3294914168071159 2308 28-29 0.288220036571154 2308 30-31 0.22270542667162374 2308 32-33 0.33388423269443024 2308 34-35 0.30835187056843694 2308 36-37 0.3388832878892316 2308 38-39 0.3599098834432368 2308 40-41 0.35638711724538297 2308 42-43 0.3331418528593346 2308 44-45 0.2973474928669546 2308 46-47 0.2608981157868726 2308 48-49 0.32353991000864823 2308 50-51 0.3599030974668693 2308 52-53 0.32643837390849484 2308 54-55 0.31215921967020677 2308 56-57 0.27213311355382075 2308 58-59 0.36621267374103184 2308 60-61 0.2794124794528088 2308 62-63 0.30350218028288367 2308 64-65 0.2831062818606256 2308 66-67 0.3153472032606146 2308 68-69 0.3100110105444429 2308 70-71 0.28701892064797363 2308 72-73 0.35116098372528626 2308 74-75 0.24935844705056098 2308 76-77 0.24362906752967461 2308 78-79 0.45130417080677177 2308 80-81 0.4826183873185741 2308 82-83 0.43482854982359953 2308 84-85 0.3289755728480017 2308 86-87 0.3742928610178424 2308 88-89 0.530804375541905 2308 90-91 0.5360538007639448 2308 92-93 0.4391528555053199 2309 1 0.545595208342462 2309 2 0.4009916060351344 2309 3 0.30961998292377046 2309 4 0.2627696696127799 2309 5 0.2914557448164601 2309 6 0.23206831272592865 2309 7 0.20959160946064515 2309 8 0.18373982540434497 2309 9 0.1568105636457915 2309 10-11 0.13905502121495772 2309 12-13 0.14505379768479543 2309 14-15 0.2183979245157346 2309 16-17 0.22404116867362234 2309 18-19 0.203535359143018 2309 20-21 0.2531161586183117 2309 22-23 0.22848643709677674 2309 24-25 0.23444616282616693 2309 26-27 0.22706431079156175 2309 28-29 0.18792748931446113 2309 30-31 0.20216427295377315 2309 32-33 0.1870105992375315 2309 34-35 0.19719169544386972 2309 36-37 0.19871551415273814 2309 38-39 0.19762095119648393 2309 40-41 0.17484957216675667 2309 42-43 0.1674116161655519 2309 44-45 0.19706879540350997 2309 46-47 0.24620404488336334 2309 48-49 0.14004698762828838 2309 50-51 0.19858091123427357 2309 52-53 0.22414288176319275 2309 54-55 0.16618337993534738 2309 56-57 0.17062764213851978 2309 58-59 0.16615869621494284 2309 60-61 0.19124315001936054 2309 62-63 0.16118248207239105 2309 64-65 0.1667297898075617 2309 66-67 0.1666278313743419 2309 68-69 0.2121444152186882 2309 70-71 0.1301273548894457 2309 72-73 0.07551178316683149 2309 74-75 0.09230418108332117 2309 76-77 0.022563792778512948 2309 78-79 0.17641711444291985 2309 80-81 0.2427441102515715 2309 82-83 0.09985488823415523 2309 84-85 0.18423061689072995 2309 86-87 0.2528139046258886 2309 88-89 0.3206983279099802 2309 90-91 0.29346120856951075 2309 92-93 0.33643098039933506 2310 1 0.5542996545270356 2310 2 0.3848300323579039 2310 3 0.3038084268787671 2310 4 0.27100190788634393 2310 5 0.2903919026624493 2310 6 0.22947404041077135 2310 7 0.1938627318856021 2310 8 0.14307704566804347 2310 9 0.1341811790351386 2310 10-11 0.10629042543669698 2310 12-13 0.10045212901220424 2310 14-15 0.21620803205638595 2310 16-17 0.1782081128691857 2310 18-19 0.16560270131176935 2310 20-21 0.1616947728003879 2310 22-23 0.19294067882754717 2310 24-25 0.1687336557146466 2310 26-27 0.13142342455292777 2310 28-29 0.09325274159365193 2310 30-31 0.07321804229624718 2310 32-33 0.07778354688788625 2310 34-35 0.06879827539750494 2310 36-37 0.06756629067547237 2310 38-39 0.12816488417932703 2310 40-41 0.0824312420986999 2310 42-43 0.08905365765763662 2310 44-45 0.0884310780407489 2310 46-47 0.05238321623394171 2310 48-49 -0.0714637295838969 2310 50-51 -0.04459138575749222 2310 52-53 0.028696118333179754 2310 54-55 0.03748462652691842 2310 56-57 0.0344403185726776 2310 58-59 0.0771531116433195 2310 60-61 0.05463214664493421 2310 62-63 -0.075305385213575 2310 64-65 -0.03498477727145399 2310 66-67 -0.09314707201255601 2310 68-69 -0.07790635243618738 2310 70-71 -0.06749871962232845 2310 72-73 -0.07233168398523304 2310 74-75 -0.11448763493158509 2310 76-77 -0.1031785294804024 2310 78-79 0.055614422967614985 2310 80-81 0.09550393285614689 2310 82-83 0.030016510020288933 2310 84-85 -0.055260513077151074 2310 86-87 0.02297530652533908 2310 88-89 0.05744121989445716 2310 90-91 7.700382427131558E-4 2310 92-93 0.05609907832552352 2311 1 0.5288333594585097 2311 2 0.3517707119572151 2311 3 0.2751286695519326 2311 4 0.25498568790570886 2311 5 0.25113058652826226 2311 6 0.20991924025020126 2311 7 0.11869032883878106 2311 8 0.1659566042299474 2311 9 0.1197134246986522 2311 10-11 0.07960511148863958 2311 12-13 0.08525296125534254 2311 14-15 0.11172834185035185 2311 16-17 0.1062218069509413 2311 18-19 0.08362001854706591 2311 20-21 0.06459626916787897 2311 22-23 0.03091341507158063 2311 24-25 0.051056117555944525 2311 26-27 0.04196762001581078 2311 28-29 0.052590028337597516 2311 30-31 0.019277424674150723 2311 32-33 0.036349366301479336 2311 34-35 0.09447514293971437 2311 36-37 0.016407654458433285 2311 38-39 0.0661218543909996 2311 40-41 0.0836424590070095 2311 42-43 0.04772612637840723 2311 44-45 -0.0735998007801939 2311 46-47 -0.016793729956674497 2311 48-49 -0.03895722274445035 2311 50-51 0.04022142294364528 2311 52-53 0.047096401672014565 2311 54-55 0.06841666190970841 2311 56-57 -0.013818032879775899 2311 58-59 -0.03586529788614712 2311 60-61 0.020293146242366333 2311 62-63 0.045900067853153814 2311 64-65 0.016273967334839767 2311 66-67 -0.06780011958070986 2311 68-69 -0.0979418486483894 2311 70-71 -0.07741616827048858 2311 72-73 -0.11797697012147523 2311 74-75 -0.23599534841890346 2311 76-77 -0.2424451408101831 2311 78-79 -7.561952458630117E-4 2311 80-81 0.0349435275811274 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-0.6509030266500417 2303 68-69 -0.7023722297293062 2303 70-71 -0.6958422437625416 2303 72-73 -0.677753682285406 2303 74-75 -0.4775216322645619 2303 76-77 -0.3465347686901623 2303 78-79 -0.6341180080238189 2303 80-81 -0.5187487542139877 2303 82-83 -0.5543545870433064 2303 84-85 -0.6207398048951802 2303 86-87 -0.5799123994331197 2303 88-89 -0.4552936655774005 2303 90-91 -0.4376572678209172 2303 92-93 -0.5972491771144348 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 3.0 11 5.0 12 27.0 13 111.0 14 386.0 15 1141.0 16 2809.0 17 6022.0 18 11390.0 19 18967.0 20 29708.0 21 45588.0 22 66441.0 23 93727.0 24 126823.0 25 166654.0 26 216744.0 27 278807.0 28 358054.0 29 460206.0 30 590598.0 31 768139.0 32 1015190.0 33 1381384.0 34 1959208.0 35 3021228.0 36 5346347.0 37 1.0932008E7 38 1.2794001E7 39 282479.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.51268949221149 24.405619951878503 10.846834409645076 24.234856146264924 2 15.915892740304088 22.766054450877622 37.938147347307435 23.37990546151086 3 15.48585030918071 27.27983140123272 31.614162586638706 25.620155702947866 4 11.581231341869422 17.49744303793985 41.64388301002685 29.277442610163884 5 11.512151776915083 37.06551188835698 38.32711828218179 13.095218052546148 6 28.084480500482872 41.09501392085569 18.86644371450132 11.954061864160117 7 25.76042119172131 32.49595895552118 26.139250584033025 15.604369268724486 8 23.085375452839013 36.480792171049345 23.544966946801555 16.888865429310083 9 22.5434108179039 15.304760983929757 26.645059393941516 35.506768804224826 10-11 22.124151593296627 27.059102753663954 31.771814291694927 19.04493136134449 12-13 21.373716218675572 26.04044934488362 31.98730330904725 20.59853112739356 14-15 19.408684777767256 27.414914796908356 27.716993425383553 25.459406999940835 16-17 19.701897556241153 31.761486524644567 28.685942318638073 19.850673600476206 18-19 19.80663900799003 30.526303281404417 30.21300741640951 19.454050294196044 20-21 20.043129372896352 30.112837047352315 29.990380408218563 19.853653171532766 22-23 20.1625514289793 29.86677812861675 29.935502491051757 20.035167951352197 24-25 19.785799012715344 29.7537031742648 30.25621997976669 20.204277833253162 26-27 19.965428491162697 30.3078949719708 29.950353005723773 19.776323531142733 28-29 20.0030632322942 30.1187318343383 29.957358655720085 19.92084627764742 30-31 19.837813380131593 29.931452427139387 30.150705446141473 20.080028746587544 32-33 19.98686971892813 30.21818976334399 30.01640991193003 19.778530605797854 34-35 19.880425804454823 29.92669181010922 30.13101042426695 20.061871961169008 36-37 19.83309518706464 30.106171729362185 30.180599448149415 19.880133635423757 38-39 19.875282083149074 30.088780009259814 30.184726586277776 19.851211321313336 40-41 19.766567041172866 29.970247440667496 30.324160102851614 19.939025415308024 42-43 19.761675138519653 30.018560513343722 30.325083475529453 19.894680872607175 44-45 19.694197713256656 29.883855650625662 30.338874545548038 20.083072090569644 46-47 19.62403891566864 29.85637424716705 30.514005448726955 20.005581388437356 48-49 19.654353218444616 30.069851574940685 30.385372558618972 19.890422647995717 50-51 19.564291109491254 30.00923713294673 30.456738193230105 19.96973356433191 52-53 19.572647403221048 29.870339660518248 30.570631316938897 19.986381619321815 54-55 19.522246346036493 29.989221382770005 30.489034417229487 19.99949785396402 56-57 19.47264419052024 29.971430240223995 30.421309583900168 20.134615985355595 58-59 19.552178465919738 29.986505438535428 30.33684910893343 20.124466986611406 60-61 19.422490842782604 29.933215814969117 30.455939342509826 20.188353999738453 62-63 19.41374689049734 29.99313718287494 30.315431336622932 20.277684590004792 64-65 19.46749053331992 29.90038611422583 30.32521584060636 20.306907511847886 66-67 19.4478160264265 29.815200598343434 30.27644328311403 20.460540092116034 68-69 19.452132809257115 29.994418890869657 30.162773916938708 20.39067438293452 70-71 19.565765870648118 29.941369001087903 30.07361198282343 20.419253145440557 72-73 19.53108822263845 29.9089995756684 30.003147014362014 20.556765187331134 74-75 19.468045566595958 29.963708247007443 30.043658607651945 20.524587578744654 76-77 19.466295572124242 29.916710992734046 30.026655252534727 20.590338182606985 78-79 19.574066196651167 29.85301452339158 29.974342789987652 20.598576489969602 80-81 19.450122126486878 29.925420767623834 29.923775629212546 20.70068147667674 82-83 19.446328317159985 29.90033536168184 29.980436306798637 20.672900014359534 84-85 19.403961900192698 29.846527703175763 30.021325441744334 20.728184954887208 86-87 19.27942899270328 29.74766378960122 30.131572808163693 20.841334409531807 88-89 19.49219901112231 29.68929901112731 30.020313931061786 20.79818804668859 90-91 19.477833861045532 29.771485298402272 29.985870829202728 20.764810011349468 92-93 19.44242648147685 29.63628397245468 30.009662748428077 20.91162679764039 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2.0 1 3.0 2 6.0 3 18.5 4 48.5 5 115.5 6 270.0 7 546.0 8 1058.0 9 1826.5 10 2773.5 11 4208.5 12 6561.0 13 10080.5 14 12161.0 15 15226.0 16 23070.0 17 35123.0 18 53721.5 19 81270.0 20 119735.0 21 171618.0 22 240030.5 23 326989.0 24 431183.5 25 553944.0 26 697739.0 27 857316.5 28 1025275.5 29 1110698.0 30 1188015.0 31 1336786.0 32 1472666.0 33 1590112.5 34 1686484.0 35 1759041.5 36 1798460.0 37 1806950.5 38 1787881.5 39 1747182.0 40 1694270.0 41 1626640.5 42 1555333.0 43 1522095.0 44 1478753.5 45 1375952.0 46 1264514.0 47 1159684.5 48 1056088.5 49 955735.0 50 859344.5 51 768770.5 52 682198.0 53 601323.0 54 529218.5 55 465505.0 56 410450.0 57 385099.0 58 364166.5 59 322957.5 60 282342.0 61 246913.5 62 216031.5 63 186876.0 64 162314.0 65 141590.5 66 123094.5 67 106897.5 68 91327.5 69 75551.0 70 61276.0 71 54433.0 72 49666.5 73 40867.0 74 33873.5 75 28611.5 76 25255.5 77 22618.0 78 18657.0 79 14905.0 80 11668.5 81 8837.5 82 6872.5 83 5030.5 84 3497.0 85 2231.5 86 1654.0 87 1386.0 88 900.0 89 475.5 90 196.0 91 92.5 92 49.0 93 26.0 94 11.0 95 5.5 96 3.0 97 1.5 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2057627426893775 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.377824243865324E-5 18-19 0.0 20-21 1.1382343034049842E-4 22-23 3.877501473137858E-5 24-25 2.3765331609554615E-5 26-27 0.004213968536452079 28-29 2.0012910829098624E-4 30-31 1.4759521736460232E-4 32-33 4.602969490692683E-4 34-35 8.830696903339766E-4 36-37 0.0012820770999891304 38-39 9.693753682844645E-4 40-41 0.0012282924021359278 42-43 8.868221111144328E-4 44-45 9.018317942362566E-4 46-47 6.766865474088972E-4 48-49 3.7524207804559914E-4 50-51 5.290913300442948E-4 52-53 0.013557496279787497 54-55 0.06418390664277292 56-57 0.09878247704550398 58-59 0.11571965364155551 60-61 0.12563605095737387 62-63 0.1296186202123645 64-65 0.1305854939667953 66-67 0.1266692174789261 68-69 0.12565856548205662 70-71 0.12403251647719235 72-73 0.1227079119416914 74-75 0.12363475987446401 76-77 0.12362600422597628 78-79 0.11030491045535751 80-81 0.09592563402465015 82-83 0.08028804582556322 84-85 0.05365211231895977 86-87 0.0329324955762086 88-89 0.015152275111481294 90-91 0.005706181200146745 92-93 0.0014471836143291942 >>END_MODULE >>Sequence Length Distribution pass #Length Count 93 3.9974195E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.82390091482364 #Duplication Level Percentage of deduplicated Percentage of total 1 88.96777454256075 66.56915946984935 2 6.930974072375476 10.372050344692687 3 1.684954387146292 3.7822458032949466 4 0.625664580813135 1.8725865840270675 5 0.39712825300551147 1.4857342526680704 6 0.2781288898553676 1.2486413097652755 7 0.19550446845642688 1.0239884883332266 8 0.13561501206515264 0.8117795380260475 9 0.11684925758762361 0.7868805544536348 >10 0.6131299187653441 8.054190494711442 >50 0.0411494802116138 2.1241408241126534 >100 0.012781766726128801 1.5384873010460682 >500 2.8597533738051843E-4 0.14753550280788272 >1k 3.861187127946967E-5 0.04386926395889984 >5k 1.3393562382621907E-5 0.07212769165166881 >10k+ 7.389660170183321E-6 0.0665825766010329 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 5.003227707274656E-6 0.0 0.0 2 5.003227707274656E-6 0.0 0.0 3 5.003227707274656E-6 2.501613853637328E-6 0.0 4 5.003227707274656E-6 2.501613853637328E-6 2.501613853637328E-6 5 7.504841560911983E-6 5.003227707274656E-6 2.501613853637328E-6 6 1.2508069268186638E-5 7.504841560911983E-6 2.501613853637328E-6 7 1.5009683121823966E-5 7.504841560911983E-6 2.501613853637328E-6 8 2.5016138536373277E-5 7.504841560911983E-6 5.003227707274656E-6 9 3.0019366243647932E-5 1.0006455414549312E-5 2.251452468273595E-5 10-11 4.127662858501591E-5 1.3758876195005303E-5 6.25403463409332E-5 12-13 6.128953941411453E-5 2.3765331609554615E-5 7.379760868230116E-5 14-15 7.25468017554825E-5 2.8768559316829268E-5 8.505487102366914E-5 16-17 8.505487102366914E-5 3.752420780455992E-5 1.250806926818664E-4 18-19 9.631213336503712E-5 4.377824243865324E-5 2.076339498518982E-4 20-21 1.0506778185276777E-4 5.003227707274655E-5 3.001936624364793E-4 22-23 1.2382988575504773E-4 5.378469785320255E-5 4.840622806788229E-4 24-25 1.388395688768717E-4 5.753711863365854E-5 0.0011619996350145387 26-27 1.4634441043778366E-4 5.8787925560477204E-5 0.006485433915554772 28-29 1.56350865852333E-4 6.0038732487295865E-5 0.03500258104009349 30-31 1.7386216282779427E-4 6.0038732487295865E-5 0.06078546422260661 32-33 1.8637023209598094E-4 6.25403463409332E-5 0.08512241459771736 34-35 2.0263072214462354E-4 6.754357404820785E-5 0.1274922484367728 36-37 2.1638959833962887E-4 7.62992225359385E-5 0.21967171571560104 38-39 2.2389443990054083E-4 8.00516433163945E-5 0.37843038490205994 40-41 2.3515170224190881E-4 9.506132643821846E-5 0.5608030380599285 42-43 2.464089645832768E-4 1.0256616799913044E-4 0.7425715514721435 44-45 2.6141864770510073E-4 1.0506778185276777E-4 0.9525107384901685 46-47 2.9519043472920465E-4 1.0506778185276777E-4 1.2032762635995546 48-49 3.0894931092421E-4 1.1257262341367975E-4 1.4589862284906552 50-51 3.227081871192153E-4 1.1757585112095441E-4 1.7129100410902582 52-53 3.352162563874019E-4 1.2007746497459173E-4 1.9540193367246044 54-55 3.814961126796925E-4 1.2633149960868505E-4 2.2011700298154846 56-57 3.9275337502106046E-4 1.3258553424277839E-4 2.4746589643643855 58-59 4.190203204842524E-4 1.3758876195005303E-4 2.756640877946385 60-61 4.7030340448381763E-4 1.4009037580369035E-4 3.035007959509879 62-63 4.778082460447296E-4 1.4134118273050902E-4 3.2984441587879383 64-65 4.965703499470095E-4 1.4759521736460235E-4 3.554240429356989 66-67 5.065768053615588E-4 1.48846024291421E-4 3.825890677723466 68-69 5.228372954102015E-4 1.5885247970597033E-4 4.110509292307199 70-71 5.390977854588441E-4 1.6885893512051962E-4 4.392922734278951 72-73 5.528566616538494E-4 1.726113559009756E-4 4.664073910681628 74-75 5.678663447756734E-4 1.726113559009756E-4 4.922909642082848 76-77 5.753711863365854E-4 1.7511296975461296E-4 5.1881907815779655 78-79 5.878792556047721E-4 1.801161974618876E-4 5.46612883636556 80-81 5.966349040925027E-4 1.8762103902279957E-4 5.746564752586012 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACTG 17610 0.0 14.615613 5 GTGTAGG 13370 0.0 12.83854 1 GTATTAG 18005 0.0 12.58233 1 TATGCCG 23940 0.0 12.535206 86-87 GTATAGG 13190 0.0 12.518297 1 ATGCCGT 20295 0.0 12.322117 86-87 GTATAAG 18415 0.0 11.994636 1 TATACAG 16590 0.0 11.976351 5 TATACCG 6355 0.0 11.698636 5 GTATAGA 21530 0.0 11.675693 1 CTTACAC 12665 0.0 11.499907 3 CTATACT 20560 0.0 11.482367 4 TACACTG 13330 0.0 11.448053 5 ATACCGT 7630 0.0 11.396203 6 TACACCG 6755 0.0 11.392069 5 GTATATA 35290 0.0 11.209469 1 TGCCGTC 17665 0.0 11.202225 86-87 GTATAAT 28025 0.0 11.177228 1 TCTATAC 21635 0.0 11.173072 3 ACTGTGC 10915 0.0 11.033434 8 >>END_MODULE