FastQCFastQC Report
Wed 5 Nov 2014
Raslatmar_M8_GGACTCCT-TATCCTCT_L003_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRaslatmar_M8_GGACTCCT-TATCCTCT_L003_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39974195
Sequences flagged as poor quality0
Sequence length93
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG176100.014.6156135
GTGTAGG133700.012.838541
GTATTAG180050.012.582331
TATGCCG239400.012.53520686-87
GTATAGG131900.012.5182971
ATGCCGT202950.012.32211786-87
GTATAAG184150.011.9946361
TATACAG165900.011.9763515
TATACCG63550.011.6986365
GTATAGA215300.011.6756931
CTTACAC126650.011.4999073
CTATACT205600.011.4823674
TACACTG133300.011.4480535
ATACCGT76300.011.3962036
TACACCG67550.011.3920695
GTATATA352900.011.2094691
TGCCGTC176650.011.20222586-87
GTATAAT280250.011.1772281
TCTATAC216350.011.1730723
ACTGTGC109150.011.0334348
Produced by FastQC (version 0.11.2)