##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename PhiX_Undetermined_L004_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 14705556 Sequences flagged as poor quality 0 Sequence length 93 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.42504615262422 33.0 33.0 33.0 27.0 33.0 2 31.55715186831426 33.0 33.0 33.0 33.0 33.0 3 31.554310765264503 33.0 33.0 33.0 27.0 33.0 4 35.3730253381783 37.0 37.0 37.0 33.0 37.0 5 35.50853806547675 37.0 37.0 37.0 33.0 37.0 6 35.524764449572665 37.0 37.0 37.0 37.0 37.0 7 35.39815699590005 37.0 37.0 37.0 33.0 37.0 8 35.426645548118 37.0 37.0 37.0 33.0 37.0 9 35.44072696061271 37.0 37.0 37.0 33.0 37.0 10-11 35.44954097621334 37.0 37.0 37.0 37.0 37.0 12-13 35.0449452234244 37.0 37.0 37.0 33.0 37.0 14-15 37.42505965092377 40.0 38.5 40.0 33.0 40.0 16-17 37.48430433368178 40.0 40.0 40.0 33.0 40.0 18-19 37.487479800151725 40.0 40.0 40.0 33.0 40.0 20-21 37.66245179713028 40.0 38.5 40.0 33.0 40.0 22-23 37.6394946916662 40.0 37.0 40.0 33.0 40.0 24-25 37.50165366069804 40.0 37.0 40.0 33.0 40.0 26-27 37.42443335702506 40.0 37.0 40.0 33.0 40.0 28-29 37.30958006620083 40.0 37.0 40.0 33.0 40.0 30-31 37.252517551869516 40.0 37.0 40.0 33.0 40.0 32-33 37.196033560376776 40.0 37.0 40.0 33.0 40.0 34-35 36.93951126363396 40.0 37.0 40.0 33.0 40.0 36-37 36.89631456301278 40.0 37.0 40.0 33.0 40.0 38-39 36.712999188878 40.0 37.0 40.0 33.0 40.0 40-41 36.70254599690077 40.0 37.0 40.0 33.0 40.0 42-43 36.70218348085581 40.0 37.0 40.0 33.0 40.0 44-45 36.451926095143904 40.0 37.0 40.0 30.0 40.0 46-47 36.35285949065782 40.0 37.0 40.0 30.0 40.0 48-49 36.23024943769552 40.0 37.0 40.0 30.0 40.0 50-51 34.81974139570105 38.5 35.0 38.5 24.5 40.0 52-53 34.90653797789081 37.0 37.0 38.5 24.5 40.0 54-55 35.8104802361774 37.0 37.0 40.0 27.0 40.0 56-57 35.98637443562147 37.0 37.0 40.0 30.0 40.0 58-59 35.93900774645991 37.0 37.0 40.0 33.0 40.0 60-61 35.804624796233476 37.0 37.0 40.0 33.0 40.0 62-63 35.51749332021176 37.0 37.0 40.0 30.0 40.0 64-65 35.26513257982221 37.0 37.0 38.5 27.0 40.0 66-67 35.02663795914959 37.0 37.0 37.0 27.0 40.0 68-69 34.838939309741164 37.0 37.0 37.0 27.0 40.0 70-71 34.432871154276654 37.0 37.0 37.0 27.0 37.0 72-73 34.10779412216716 37.0 33.0 37.0 27.0 37.0 74-75 33.893031994166016 37.0 33.0 37.0 27.0 37.0 76-77 33.601720941391136 37.0 33.0 37.0 22.0 37.0 78-79 33.4590094043367 37.0 33.0 37.0 22.0 37.0 80-81 33.470291364705965 37.0 33.0 37.0 27.0 37.0 82-83 33.498514235027905 37.0 33.0 37.0 27.0 37.0 84-85 33.578970390510904 37.0 33.0 37.0 27.0 37.0 86-87 33.394746550215444 37.0 33.0 37.0 27.0 37.0 88-89 33.32316306163466 37.0 33.0 37.0 27.0 37.0 90-91 33.28811933394426 37.0 33.0 37.0 27.0 37.0 92-93 32.452139721884706 35.0 33.0 37.0 24.5 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2304 1 -2.0111856753529835 2304 2 -2.211707723016403 2304 3 -1.7579062157435636 2304 4 -2.7695679975961127 2304 5 -3.7041254879288132 2304 6 -3.1688929848225627 2304 7 -3.3810426150465496 2304 8 -2.9513488531149648 2304 9 -4.017366143734808 2304 10-11 -3.5844078650046924 2304 12-13 -3.805002078034608 2304 14-15 -5.790845482571733 2304 16-17 -5.431361671527064 2304 18-19 -6.120969696686565 2304 20-21 -7.783678572060474 2304 22-23 -7.80686984115799 2304 24-25 -9.132346968190973 2304 26-27 -11.252968686454619 2304 28-29 -8.716365917221676 2304 30-31 -13.221718807317263 2304 32-33 -16.636534321123396 2304 34-35 -15.814018052226487 2304 36-37 -20.513095900779057 2304 38-39 -25.244987754217167 2304 40-41 -25.779839236593855 2304 42-43 -26.088482695889464 2304 44-45 -25.17580534478542 2304 46-47 -25.402537033421762 2304 48-49 -25.998907847122066 2304 50-51 -22.363033951030864 2304 52-53 -14.187225031670437 2304 54-55 -8.737266108952483 2304 56-57 -7.284708794568019 2304 58-59 -7.1947050040424685 2304 60-61 -6.977842416557621 2304 62-63 -6.614713082708533 2304 64-65 -6.654225631337052 2304 66-67 -6.789554451542635 2304 68-69 -6.616887109597926 2304 70-71 -6.540609968419556 2304 72-73 -6.450611793873055 2304 74-75 -6.6444158601664896 2304 76-77 -6.530669448276647 2304 78-79 -6.606980874739378 2304 80-81 -6.887198795400828 2304 82-83 -7.173866346804331 2304 84-85 -7.366251013821373 2304 86-87 -7.057228237452584 2304 88-89 -7.277305340620227 2304 90-91 -7.474894739260378 2304 92-93 -7.467977581103661 2305 1 -1.1841029173299447 2305 2 -1.4490346214645875 2305 3 -1.1265119970846378 2305 4 -1.7392348174542747 2305 5 -2.3087408387276582 2305 6 -1.6336862685164633 2305 7 -1.9543571274693363 2305 8 -1.6573929778251397 2305 9 -2.406807400690006 2305 10-11 -1.9855942948485819 2305 12-13 -2.2180939886391613 2305 14-15 -4.209562569874969 2305 16-17 -4.100770836589604 2305 18-19 -5.2556299513752265 2305 20-21 -5.323582725891349 2305 22-23 -5.145384609568069 2305 24-25 -5.715795616235987 2305 26-27 -7.532067091738689 2305 28-29 -6.551116040326498 2305 30-31 -8.274771112150326 2305 32-33 -8.296277262587147 2305 34-35 -8.690059390182014 2305 36-37 -10.102431220633058 2305 38-39 -18.77163751676192 2305 40-41 -15.578918773805619 2305 42-43 -18.124037314385784 2305 44-45 -18.80813816666632 2305 46-47 -19.665802099266738 2305 48-49 -21.950116144020832 2305 50-51 -19.619285136917505 2305 52-53 -11.894452453336228 2305 54-55 -6.21187127028486 2305 56-57 -5.815725272802403 2305 58-59 -6.141009825481731 2305 60-61 -5.676136179911872 2305 62-63 -5.562016133307964 2305 64-65 -5.448713844981842 2305 66-67 -5.481603155828047 2305 68-69 -5.471027031967985 2305 70-71 -5.2145716749240165 2305 72-73 -5.063361284841871 2305 74-75 -5.367432659774572 2305 76-77 -5.230443248689937 2305 78-79 -5.151711824062637 2305 80-81 -5.744736132986468 2305 82-83 -5.805309958984093 2305 84-85 -6.3623730539683265 2305 86-87 -5.925678763967305 2305 88-89 -6.091692195223139 2305 90-91 -6.4588087475408 2305 92-93 -6.608422183427706 2306 1 -0.6641333140372616 2306 2 -0.8036863117817177 2306 3 -0.9829826017929761 2306 4 -1.6733850001498212 2306 5 -1.7103331856326491 2306 6 -1.8671850674695847 2306 7 -1.7618105303839187 2306 8 -1.4206856713655185 2306 9 -1.5613063160481744 2306 10-11 -1.4055368096809673 2306 12-13 -1.6501863589951071 2306 14-15 -3.0086905898267275 2306 16-17 -3.1996916078783926 2306 18-19 -3.504979786474003 2306 20-21 -4.92492803993904 2306 22-23 -5.090050487399694 2306 24-25 -4.466191714259843 2306 26-27 -5.65384675411638 2306 28-29 -6.420734314228703 2306 30-31 -6.4838871387554065 2306 32-33 -6.328631598824622 2306 34-35 -5.453199861744444 2306 36-37 -5.675445807587558 2306 38-39 -7.314707775461407 2306 40-41 -6.781774142050267 2306 42-43 -6.113134735462392 2306 44-45 -6.8822637381550855 2306 46-47 -8.327305381729115 2306 48-49 -8.93399120470606 2306 50-51 -11.008782330297834 2306 52-53 -7.841265343405048 2306 54-55 -5.049708215510137 2306 56-57 -4.768583260097081 2306 58-59 -4.908280672111658 2306 60-61 -4.911285513071295 2306 62-63 -4.530625239672624 2306 64-65 -4.573350351911316 2306 66-67 -4.430045513555477 2306 68-69 -4.727624850214333 2306 70-71 -4.790394073755188 2306 72-73 -4.8348095606584565 2306 74-75 -4.892191953857264 2306 76-77 -4.653676419150067 2306 78-79 -4.784274007603074 2306 80-81 -4.922488115975149 2306 82-83 -5.175809375170573 2306 84-85 -5.54902427724479 2306 86-87 -5.7026235062731985 2306 88-89 -5.700200744271704 2306 90-91 -5.783587083956242 2306 92-93 -5.7834722960646126 2307 1 0.11680400775028232 2307 2 -0.2413062819946603 2307 3 -0.006342551119924877 2307 4 -0.1895601995297227 2307 5 -0.9025426203674485 2307 6 -0.30161844798883664 2307 7 -0.4138569244540946 2307 8 -0.1324875036385862 2307 9 -0.18602912779428493 2307 10-11 0.11351947518785721 2307 12-13 0.7902016329452834 2307 14-15 -0.6869524533399627 2307 16-17 -0.49310839234982495 2307 18-19 -0.27442481304078115 2307 20-21 -0.39243689159930994 2307 22-23 -0.6503686438634588 2307 24-25 0.01500177336351527 2307 26-27 -0.30744835502883205 2307 28-29 0.12349482096635 2307 30-31 -0.628228793597593 2307 32-33 0.21127546804454767 2307 34-35 0.25098116894096023 2307 36-37 0.6181858220357199 2307 38-39 0.1403482591148162 2307 40-41 0.6763763828640492 2307 42-43 0.8985742052885968 2307 44-45 0.9557422171764856 2307 46-47 0.6752930443561098 2307 48-49 1.0080109664702377 2307 50-51 -0.5402451425224513 2307 52-53 1.2351040663901145 2307 54-55 2.0923015092536232 2307 56-57 1.9676391043950403 2307 58-59 1.767493988691058 2307 60-61 1.7825494632873387 2307 62-63 1.8498600431144112 2307 64-65 1.8290214105382816 2307 66-67 1.725241241508968 2307 68-69 1.6721786924981998 2307 70-71 1.8485053973576697 2307 72-73 1.9665891412927436 2307 74-75 2.001514467484249 2307 76-77 2.22450057852776 2307 78-79 2.2833514445619514 2307 80-81 2.0685594425797547 2307 82-83 1.8596510371896855 2307 84-85 1.684189174894314 2307 86-87 1.9113184397661769 2307 88-89 1.8191998929662176 2307 90-91 1.726349524321357 2307 92-93 1.8193213470474205 2308 1 1.5646351825340368 2308 2 1.1466987789658454 2308 3 1.3077876905642292 2308 4 1.4607529642627952 2308 5 1.2879883057076 2308 6 1.34907864401098 2308 7 1.5919167157278835 2308 8 1.5504178488750568 2308 9 1.5921099937657601 2308 10-11 1.3784429674302743 2308 12-13 2.055379256114499 2308 14-15 1.645177694399159 2308 16-17 1.8530330610907129 2308 18-19 1.370320672384942 2308 20-21 1.5112357001523264 2308 22-23 1.435055673514043 2308 24-25 1.8353833109062592 2308 26-27 1.9491380174098012 2308 28-29 2.046296192625576 2308 30-31 2.054832888334161 2308 32-33 2.2385317284271693 2308 34-35 2.5174360578468296 2308 36-37 2.462859863338288 2308 38-39 2.735945277372764 2308 40-41 2.780900888182849 2308 42-43 2.864859536254677 2308 44-45 2.980345895432592 2308 46-47 3.037360503752943 2308 48-49 3.0395314442168626 2308 50-51 3.2826543022480905 2308 52-53 3.3298992393054334 2308 54-55 2.9774278666201965 2308 56-57 2.578319777981058 2308 58-59 2.3099209435686703 2308 60-61 2.360288970360287 2308 62-63 2.4680002652616366 2308 64-65 2.4841630794853202 2308 66-67 2.484649954733136 2308 68-69 2.3955107834074383 2308 70-71 2.6087716169172808 2308 72-73 2.802849157841422 2308 74-75 2.8578666005547646 2308 76-77 3.0824708316116514 2308 78-79 3.093910002940696 2308 80-81 2.9901223518851765 2308 82-83 2.1419104126859807 2308 84-85 2.121368129800679 2308 86-87 2.693336152089266 2308 88-89 2.802127692349039 2308 90-91 2.7648831538180474 2308 92-93 2.6110958187264544 2309 1 0.6520010945940129 2309 2 0.5777635087943338 2309 3 0.6310735160069569 2309 4 0.5815160064469325 2309 5 0.27591626749272535 2309 6 0.10711200591484982 2309 7 0.32247545110568865 2309 8 0.2529606931160302 2309 9 0.3341887490266444 2309 10-11 0.3696804470061039 2309 12-13 0.728501787085257 2309 14-15 0.825347428052531 2309 16-17 1.0015701529690304 2309 18-19 1.0337778064079828 2309 20-21 0.531068797470148 2309 22-23 0.45278609058659214 2309 24-25 0.7489076842540712 2309 26-27 0.8498979975500589 2309 28-29 0.763689384313686 2309 30-31 1.0610780606997992 2309 32-33 1.2172589436273924 2309 34-35 1.5856367760979566 2309 36-37 1.2601097874352334 2309 38-39 1.5274957022593227 2309 40-41 1.461709563072482 2309 42-43 1.4517906593751633 2309 44-45 1.4253253117700524 2309 46-47 1.3453690295531757 2309 48-49 1.7028765061830455 2309 50-51 2.2897209073378804 2309 52-53 1.9542140406795525 2309 54-55 1.6916945793145572 2309 56-57 1.1659351998250287 2309 58-59 0.9511198598077044 2309 60-61 1.1431615272966624 2309 62-63 1.2131455210582232 2309 64-65 0.8526960913248942 2309 66-67 0.8386597054591789 2309 68-69 0.9468285355412505 2309 70-71 1.1060243990501561 2309 72-73 1.2767802164696462 2309 74-75 1.5226495757592176 2309 76-77 1.785307126736079 2309 78-79 1.5037234313539258 2309 80-81 1.221423545092918 2309 82-83 0.969960446285647 2309 84-85 1.0620525171761912 2309 86-87 1.4380308883214568 2309 88-89 1.0685282731846968 2309 90-91 0.9654365265894356 2309 92-93 1.0174016846605412 2310 1 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-1.6716558338826957 2310 60-61 -1.2816002754010967 2310 62-63 -1.3185723057375824 2310 64-65 -1.3268508848111722 2310 66-67 -1.3696633490825647 2310 68-69 -1.4937355228236342 2310 70-71 -1.228915394845103 2310 72-73 -1.3372926943584247 2310 74-75 -1.0166468686616241 2310 76-77 -1.0052701363614887 2310 78-79 -1.3499064303285024 2310 80-81 -1.2504239401692203 2310 82-83 -1.306627755728318 2310 84-85 -1.5066524628462972 2310 86-87 -1.351479692265869 2310 88-89 -1.3293639935043728 2310 90-91 -1.11506465391518 2310 92-93 -1.4750015683359976 2311 1 -1.3191988256941016 2311 2 -1.0114367826257507 2311 3 -1.137483026399842 2311 4 -1.5671120408085955 2311 5 -1.7194344002168904 2311 6 -2.4106143556051904 2311 7 -2.1380931016140963 2311 8 -2.1522520151264715 2311 9 -2.5803792078414887 2311 10-11 -2.237801641834892 2311 12-13 -2.353743178435309 2311 14-15 -2.1298624181940298 2311 16-17 -2.0488661126006065 2311 18-19 -1.9257178058938607 2311 20-21 -2.483332279196823 2311 22-23 -2.949363626118867 2311 24-25 -2.764786310476815 2311 26-27 -2.363170220442079 2311 28-29 -2.4915293725452514 2311 30-31 -2.286851924544358 2311 32-33 -2.014722675203643 2311 34-35 -1.5138920678193202 2311 36-37 -1.6038627384636683 2311 38-39 -2.0637103183201475 2311 40-41 -1.873205098104627 2311 42-43 -2.1222434782739867 2311 44-45 -2.0355224876167526 2311 46-47 -2.884802469591442 2311 48-49 -2.5899107207820435 2311 50-51 -0.9560624716339134 2311 52-53 -0.2927929839967547 2311 54-55 -1.014273010171344 2311 56-57 -1.9088443353934537 2311 58-59 -2.457562435520998 2311 60-61 -3.043823628948111 2311 62-63 -2.379456871981141 2311 64-65 -2.203715246657545 2311 66-67 -2.098993385652136 2311 68-69 -1.9169815157752694 2311 70-71 -1.5051832598894919 2311 72-73 -1.6593263997693555 2311 74-75 -1.5355600761763597 2311 76-77 -1.0734848055623516 2311 78-79 -1.4427034495190085 2311 80-81 -1.4574109978201548 2311 82-83 -1.7267866965712102 2311 84-85 -2.1534240107801708 2311 86-87 -1.770614943058888 2311 88-89 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2.4304971042466903 1113 16-17 2.1149513919772147 1113 18-19 1.2443295474957168 1113 20-21 1.2989221497675985 1113 22-23 1.4598016529268278 1113 24-25 1.9438643993517886 1113 26-27 2.177815363689895 1113 28-29 1.9856339629441209 1113 30-31 2.386437629149661 1113 32-33 2.538255094430042 1113 34-35 2.55907986126892 1113 36-37 2.62684996293914 1113 38-39 3.007358198009186 1113 40-41 2.4297565503387375 1113 42-43 2.4033683586542622 1113 44-45 2.9631528203914783 1113 46-47 3.0206867801276402 1113 48-49 3.0985305128167084 1113 50-51 3.5070200528951077 1113 52-53 3.4661272697366385 1113 54-55 2.8565208226499195 1113 56-57 2.386232169183451 1113 58-59 1.8405365489231258 1113 60-61 2.0993334735257463 1113 62-63 2.278993402908533 1113 64-65 2.2827788683768446 1113 66-67 2.2834397356198224 1113 68-69 2.167320790990871 1113 70-71 2.416768103162539 1113 72-73 2.6486558499970485 1113 74-75 2.6828158417905783 1113 76-77 2.873806930353517 1113 78-79 2.8694015723288615 1113 80-81 2.1878421566473065 1113 82-83 2.3485803787370614 1113 84-85 1.6942452678309579 1113 86-87 2.318189802405854 1113 88-89 2.4784822202490346 1113 90-91 2.4819626137185153 1113 92-93 2.30795041864107 1114 1 1.476952508620709 1114 2 1.3750421458423894 1114 3 1.327106540983653 1114 4 1.3477966948662612 1114 5 1.1579906220269933 1114 6 1.2665825277489589 1114 7 1.504094288337619 1114 8 1.1089628320767773 1114 9 1.3061615668965842 1114 10-11 1.2092492833991528 1114 12-13 1.870495943982739 1114 14-15 2.2245298392806703 1114 16-17 1.815455226587133 1114 18-19 1.5238751718037307 1114 20-21 1.4563298213997342 1114 22-23 1.472808727804619 1114 24-25 1.7756892624158027 1114 26-27 1.6482854210592777 1114 28-29 1.950982687246615 1114 30-31 2.100367354544133 1114 32-33 2.210560476866277 1114 34-35 2.146085969370226 1114 36-37 2.2695299485195264 1114 38-39 2.641726518038219 1114 40-41 2.6537937433393495 1114 42-43 2.703338502049114 1114 44-45 2.907932981638133 1114 46-47 2.8951545112508867 1114 48-49 2.005764332638826 1114 50-51 2.836233513906244 1114 52-53 2.9933665084872203 1114 54-55 2.4624627953943232 1114 56-57 1.9682482400726045 1114 58-59 1.7779796650349482 1114 60-61 1.838238635845677 1114 62-63 1.9476100511290895 1114 64-65 1.6650474577893775 1114 66-67 1.6352374539811336 1114 68-69 1.1512099275994458 1114 70-71 1.2060630735266997 1114 72-73 0.6439083170711939 1114 74-75 0.5891440655760292 1114 76-77 1.0142494683389032 1114 78-79 1.6223139878983375 1114 80-81 1.7094523799849526 1114 82-83 1.5675911821072717 1114 84-85 0.5678563583922127 1114 86-87 1.1971894580509073 1114 88-89 1.462841199171045 1114 90-91 1.5190816471805348 1114 92-93 1.3987118702793566 1115 1 1.3394712139845346 1115 2 1.259218531933957 1115 3 1.0141119076472656 1115 4 1.1613869243259671 1115 5 1.1222703603750972 1115 6 0.7109809441435999 1115 7 1.1007022295709703 1115 8 1.1874486123332204 1115 9 1.242040344820964 1115 10-11 1.2211206221187396 1115 12-13 1.7365626897053161 1115 14-15 2.0113994389797725 1115 16-17 1.9367943507926526 1115 18-19 1.2959898108634391 1115 20-21 1.3568394353251918 1115 22-23 1.4174925885944347 1115 24-25 1.3724853721166355 1115 26-27 1.6890658871135997 1115 28-29 1.7807654723269053 1115 30-31 1.9729326041997908 1115 32-33 1.2846003151601195 1115 34-35 1.8927256645630024 1115 36-37 2.1306821589781038 1115 38-39 2.5086031193178684 1115 40-41 2.525077932493346 1115 42-43 2.5441293377133647 1115 44-45 1.6727730682638722 1115 46-47 2.132780322280702 1115 48-49 1.8126652695662457 1115 50-51 2.453468479035749 1115 52-53 2.77403850629387 1115 54-55 2.2141280022948635 1115 56-57 1.8143832333795658 1115 58-59 1.5328035624156087 1115 60-61 1.1745892310218977 1115 62-63 1.232798349641115 1115 64-65 1.3063962026885534 1115 66-67 1.5888375919528528 1115 68-69 1.5892019634402246 1115 70-71 1.8624643649132366 1115 72-73 2.0434600097011923 1115 74-75 1.8225582638333648 1115 76-77 2.005226473134684 1115 78-79 2.207749959031659 1115 80-81 2.1084971308843308 1115 82-83 1.690581034580383 1115 84-85 1.3417965275538037 1115 86-87 1.1768709985244854 1115 88-89 1.4524177463820394 1115 90-91 1.6335477131130531 1115 92-93 1.590605154950751 1116 1 1.4633328725700245 1116 2 1.3273855839527506 1116 3 1.262673571470561 1116 4 1.2984789935546601 1116 5 1.2264800517940913 1116 6 1.2715165087780065 1116 7 1.4805057561041508 1116 8 1.1267731985473404 1116 9 0.7111339068215017 1116 10-11 0.8507173585977412 1116 12-13 1.5415209837551842 1116 14-15 2.019028101695646 1116 16-17 1.4387839930511888 1116 18-19 0.7597202158947098 1116 20-21 0.46561547465355346 1116 22-23 0.03395753464513973 1116 24-25 -0.08135632672506432 1116 26-27 0.5991050583446338 1116 28-29 1.2478484315319562 1116 30-31 1.5324986247469212 1116 32-33 1.679522936078385 1116 34-35 1.9538310078631156 1116 36-37 1.9767272769781599 1116 38-39 2.2355105936526627 1116 40-41 2.234576301294041 1116 42-43 2.2732106283062876 1116 44-45 2.5020332670008187 1116 46-47 2.48964908369161 1116 48-49 2.4679398236053345 1116 50-51 2.9251379703709333 1116 52-53 2.5110576018013333 1116 54-55 1.5378756172270656 1116 56-57 1.0836758877448887 1116 58-59 1.046513315565761 1116 60-61 1.3898482587373167 1116 62-63 1.5712839438316522 1116 64-65 1.6008082413883855 1116 66-67 1.5609572157294593 1116 68-69 1.407840641570182 1116 70-71 1.6674398688640366 1116 72-73 1.8728730211345521 1116 74-75 1.9135686533757408 1116 76-77 2.0693236361199254 1116 78-79 2.07855360528589 1116 80-81 1.8181785980657992 1116 82-83 1.739811293701628 1116 84-85 1.6427273867768193 1116 86-87 1.1904291081291092 1116 88-89 1.0932557095528495 1116 90-91 0.9090513739582846 1116 92-93 1.0741711493088957 2301 1 1.5153373870590237 2301 2 1.371118007498989 2301 3 1.2983474948675244 2301 4 1.485428770550989 2301 5 1.3244925883248584 2301 6 0.3684895171959326 2301 7 0.9260545188755671 2301 8 1.1129776705023957 2301 9 1.2276383579089583 2301 10-11 0.7987435339474231 2301 12-13 1.6017591538769835 2301 14-15 1.6409965409594705 2301 16-17 1.303704177005507 2301 18-19 0.7916162775609479 2301 20-21 0.488455199560768 2301 22-23 1.0441111710478026 2301 24-25 1.4553569225987317 2301 26-27 1.1060411428553891 2301 28-29 1.1126689702353545 2301 30-31 1.5238473651530668 2301 32-33 1.6364753240199121 2301 34-35 1.9125804075454127 2301 36-37 1.9364764477659335 2301 38-39 2.1625108734681078 2301 40-41 1.490081289547284 2301 42-43 1.3802012044415477 2301 44-45 1.8643733778087253 2301 46-47 1.8072863367875485 2301 48-49 2.1506420308275196 2301 50-51 2.674406161869534 2301 52-53 2.6748369986480256 2301 54-55 1.7741057618125282 2301 56-57 0.6875832870537621 2301 58-59 0.9762458821398781 2301 60-61 1.2062819384826753 2301 62-63 1.0430465340290809 2301 64-65 1.2760645196382612 2301 66-67 1.3255438838838103 2301 68-69 1.2391900671622338 2301 70-71 1.0987075114650793 2301 72-73 1.4488202051577659 2301 74-75 1.6478870344299636 2301 76-77 1.8435720633125001 2301 78-79 1.4317497454302455 2301 80-81 1.0866294039642383 2301 82-83 1.087849002390037 2301 84-85 1.0192152373948105 2301 86-87 1.3329934981314366 2301 88-89 1.0403885272869076 2301 90-91 1.05742061527652 2301 92-93 0.5593488121484995 2302 1 1.5403016097794655 2302 2 1.4206425695710614 2302 3 1.3570745906744541 2302 4 1.5540120326704923 2302 5 1.1627520350988547 2302 6 1.3028546287092908 2302 7 1.4347060643506637 2302 8 1.4063784493131948 2302 9 1.4910838759594611 2302 10-11 1.0678275787125244 2302 12-13 1.6692402248428024 2302 14-15 1.9963906053965061 2302 16-17 1.9798746701164163 2302 18-19 1.9947935806651316 2302 20-21 1.4977057666331817 2302 22-23 1.499428474035014 2302 24-25 1.7824750631099207 2302 26-27 1.6105425634742119 2302 28-29 1.9102371016478301 2302 30-31 2.0943235530485396 2302 32-33 1.873377528967417 2302 34-35 2.1702028962228255 2302 36-37 2.3432153016706465 2302 38-39 2.637465571573891 2302 40-41 2.607769394393067 2302 42-43 2.6846408732460034 2302 44-45 2.4974234915236977 2302 46-47 2.6979644045259548 2302 48-49 2.7838122222969304 2302 50-51 3.286363568041409 2302 52-53 2.8483956544361106 2302 54-55 2.1111823239298175 2302 56-57 1.5614417574819868 2302 58-59 1.616021223172993 2302 60-61 1.7729034812226203 2302 62-63 1.7111830311093499 2302 64-65 1.684767159894264 2302 66-67 1.8545684160054776 2302 68-69 1.7690178676471149 2302 70-71 1.9907246258657665 2302 72-73 1.9531309982192013 2302 74-75 1.9824617201080628 2302 76-77 2.3986528376610536 2302 78-79 2.5105329719616165 2302 80-81 2.2972478801811462 2302 82-83 1.905515598880278 2302 84-85 1.8720117097338473 2302 86-87 2.030192690852992 2302 88-89 2.0924122314483355 2302 90-91 2.069959175422184 2302 92-93 1.8571810766126333 2303 1 1.573713024076941 2303 2 1.5056205300750243 2303 3 1.4600199758592147 2303 4 1.1561672030738563 2303 5 1.235780242784422 2303 6 1.3751380414136207 2303 7 1.5395647785434576 2303 8 1.2922845961714131 2303 9 1.4747346430982944 2303 10-11 1.5283707317440687 2303 12-13 2.069887464375185 2303 14-15 2.0916107721656445 2303 16-17 1.718003981285264 2303 18-19 2.058986592203347 2303 20-21 1.9370022391389128 2303 22-23 1.9475585840208325 2303 24-25 2.1489182115776444 2303 26-27 2.203343758357981 2303 28-29 2.328102799673651 2303 30-31 2.4353495056259717 2303 32-33 2.443620280175729 2303 34-35 2.685941854762696 2303 36-37 2.694577243278836 2303 38-39 2.957938725094877 2303 40-41 2.93835342760638 2303 42-43 3.0315588832742577 2303 44-45 3.2321458837331463 2303 46-47 3.194999202422011 2303 48-49 3.185736612528885 2303 50-51 3.627910741312718 2303 52-53 3.6345755481361763 2303 54-55 2.9558946898940306 2303 56-57 2.5161961856172965 2303 58-59 2.3150573232647034 2303 60-61 2.2937833022498353 2303 62-63 1.7761158014804153 2303 64-65 1.6906254905014038 2303 66-67 2.074929929983867 2303 68-69 2.106227473618979 2303 70-71 2.4191447418365115 2303 72-73 2.392435473654757 2303 74-75 2.0757107277713303 2303 76-77 2.702909162381509 2303 78-79 2.8365708982327575 2303 80-81 2.741953170150232 2303 82-83 2.6919657767011884 2303 84-85 2.5250663344678728 2303 86-87 2.6389725720246773 2303 88-89 2.6607923132866347 2303 90-91 2.423429633688663 2303 92-93 2.214432702246498 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 56046.0 3 1782.0 4 1414.0 5 1199.0 6 1448.0 7 7420.0 8 16979.0 9 16453.0 10 21293.0 11 31643.0 12 45717.0 13 58529.0 14 65642.0 15 65639.0 16 62384.0 17 56687.0 18 51815.0 19 49905.0 20 50208.0 21 54392.0 22 70181.0 23 113925.0 24 87922.0 25 71096.0 26 100082.0 27 180398.0 28 133189.0 29 185803.0 30 145869.0 31 159186.0 32 195086.0 33 251744.0 34 349431.0 35 591134.0 36 1524173.0 37 5655045.0 38 4174637.0 39 60.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 21.476451460610107 28.61755998050295 27.944100802584725 21.96188775630221 2 21.989466355668018 29.724663073209435 28.436777721559015 19.849092849563533 3 21.373165492284475 27.61568227643495 28.668639304140598 22.342512927139975 4 22.72033592191766 26.879873940379355 28.170231640434107 22.22955849726888 5 23.030092788239692 27.676732698588797 28.462436258174666 20.83073825499684 6 23.012561420789012 26.780598204800736 27.643652700027687 22.56318767438257 7 22.850117158747985 27.046492603542617 28.34966331038445 21.75372692732495 8 22.224382431559413 26.96647381674856 28.687613395335138 22.121530356356892 9 22.145228908460112 26.351426302902002 29.106840068174456 22.39650472046343 10-11 22.12961442136496 27.259440840057252 28.57341099942685 22.037533739150934 12-13 22.22697422055134 26.91305123536306 28.769472024530558 22.09050251955504 14-15 21.873523746965557 27.099008408614377 28.965379737900594 22.06208810651947 16-17 22.106563865036883 27.04244779552112 28.79479826520543 22.05619007423657 18-19 22.112695120113237 26.551505838348472 28.83349266730878 22.50230637422951 20-21 22.148808380791507 26.610654003626934 28.831543539465105 22.408994076116453 22-23 22.32784084619281 26.818961677017278 28.536880835216987 22.316316641572925 24-25 22.26592923350336 26.58127851852606 28.56058233993982 22.592209908030757 26-27 22.41562402266532 26.687125112430838 28.4302172024837 22.467033662420143 28-29 22.5208097219201 26.74554027131561 28.200766679088645 22.532883327675645 30-31 22.264591881980024 26.5565035019779 28.534232464989273 22.644672151052806 32-33 22.530825484349716 26.532010380962294 28.470638966975653 22.46652516771233 34-35 22.491557567270476 26.6728887189233 28.361942272225445 22.473611441580786 36-37 22.56197719379157 26.44011116223358 28.382147704843863 22.615763939130986 38-39 22.528301241183026 26.51230817097162 28.480984452881604 22.478406134963752 40-41 22.342028051119257 26.76417035017457 28.301477136753306 22.59232446195287 42-43 22.347524831624664 26.564915077378604 28.498600726693972 22.58895936430276 44-45 22.57218796989939 26.47668298408873 28.442939001951324 22.50819004406056 46-47 22.560562417207823 26.58365434865693 28.283050418232815 22.572732815902434 48-49 22.406496340766623 26.683794206054756 28.30679354645134 22.60291590672729 50-51 22.55975400447508 26.413073586403975 28.620066096341233 22.40710631277971 52-53 22.539713136916106 26.681246356884735 28.40872892039333 22.37031158580583 54-55 22.509251048466965 26.4966043646412 28.46018353590946 22.533961050982377 56-57 22.549163930900693 26.39919675325973 28.53297634996578 22.5186629658738 58-59 22.563151700244326 26.678170253194512 28.44532957519134 22.313348471369824 60-61 22.53719219867896 26.492881021014185 28.288937947969895 22.68098883233696 62-63 22.466052636506692 26.445350509338596 28.502745872784878 22.585850981369834 64-65 22.47781858499396 26.42858062824057 28.558309403985255 22.53529138278021 66-67 22.302030725539332 26.43090381464696 28.52895913184964 22.738106327964065 68-69 22.49178724401199 26.43579216013765 28.575520072898897 22.496900522951467 70-71 22.395741278414988 26.543227649121327 28.53688209494205 22.524148977521634 72-73 22.414507591527105 26.37881920051693 28.464136920364187 22.74253628759178 74-75 22.43706673903231 26.26895392650504 28.569587063263636 22.724392271199015 76-77 22.364246801629257 26.72832174020495 28.384020069451527 22.52341138871427 78-79 22.335866163323345 26.460172144891846 28.510477529912215 22.693484161872593 80-81 22.370547892173338 26.570763662486137 28.52217837126421 22.53651007407631 82-83 22.304635427579825 26.755932623163975 28.447426787618795 22.492005161637405 84-85 22.39884267171469 26.567134247655478 28.462920381751616 22.571102698878217 86-87 22.287895754770624 26.591696699711125 28.494029208376194 22.626378337142057 88-89 22.42102553775906 26.64371836303239 28.498606039147727 22.436650060060813 90-91 22.38618039966203 26.44129519271875 28.61968581701807 22.55283859060115 92-93 22.68656629771758 26.305073227785154 28.628108712633765 22.380251761863498 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 53892.0 1 27853.5 2 1476.0 3 1079.0 4 971.0 5 896.0 6 865.5 7 867.5 8 894.5 9 945.5 10 989.0 11 1028.5 12 1072.5 13 1181.0 14 1273.0 15 1375.0 16 1625.0 17 1899.5 18 2228.5 19 2601.5 20 3015.0 21 3592.5 22 4202.0 23 4772.0 24 5396.0 25 6015.0 26 6785.0 27 7862.5 28 8928.0 29 9396.0 30 10295.0 31 13699.5 32 27757.0 33 51026.0 34 71234.0 35 118683.5 36 236171.5 37 373556.5 38 481501.0 39 603314.0 40 832901.5 41 1033455.0 42 1160840.0 43 1244535.0 44 1284795.0 45 1402094.5 46 1481233.0 47 1361742.0 48 1142913.0 49 898230.0 50 588099.5 51 375913.5 52 275069.5 53 210195.5 54 163161.5 55 106324.0 56 76746.0 57 70501.0 58 50935.0 59 23434.0 60 14114.0 61 12023.5 62 10511.0 63 9148.0 64 8033.0 65 7077.5 66 6223.0 67 5533.0 68 5057.0 69 4620.5 70 4200.5 71 3973.0 72 3855.0 73 3539.5 74 3181.5 75 2843.5 76 2560.5 77 2357.0 78 2096.5 79 1865.5 80 1716.0 81 1516.0 82 1305.5 83 1157.5 84 1037.5 85 922.0 86 870.0 87 859.5 88 786.0 89 669.0 90 616.5 91 585.5 92 535.5 93 483.5 94 417.0 95 347.0 96 288.5 97 248.5 98 218.5 99 213.5 100 219.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5562795449556617 2 0.5584759936992522 3 0.5632701000900612 4 0.5619236702100893 5 0.5557083322793099 6 0.4735081080919348 7 0.4808522710735997 8 0.4559977195013912 9 0.4451922797070713 10-11 0.45030939326605535 12-13 0.4256452459192974 14-15 0.4090868784560067 16-17 0.3948031614717594 18-19 0.39881865058349375 20-21 0.42014052375850325 22-23 0.42475782622567965 24-25 0.4343086381772984 26-27 0.4374741084254142 28-29 0.45006458783333314 30-31 0.4525024419341914 32-33 0.46235586060125844 34-35 0.45764335602135686 36-37 0.4442674591834542 38-39 0.43411823395184784 40-41 0.43327841531459266 42-43 0.43175178143553367 44-45 0.4383139270626694 46-47 0.43161917849280906 48-49 0.4261416569356507 50-51 0.42628786018019316 52-53 0.4473445274697536 54-55 0.4745315308037316 56-57 0.4756943566091619 58-59 0.49259273161790007 60-61 0.4969924292559901 62-63 0.4967510238987224 64-65 0.4755787540437097 66-67 0.46679295906934765 68-69 0.4569667410059164 70-71 0.44784093848610695 72-73 0.4435126424325609 74-75 0.45515790086413593 76-77 0.44876235893426947 78-79 0.4738855164673815 80-81 0.4923513262606324 82-83 0.4963430148441854 84-85 0.4768503822636832 86-87 0.46758857672569465 88-89 0.46450810836394085 90-91 0.44684811645340033 92-93 0.4334654194645887 >>END_MODULE >>Sequence Length Distribution pass #Length Count 93 1.4705556E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.39074067573544 #Duplication Level Percentage of deduplicated Percentage of total 1 99.80324776102712 99.1951871681242 2 0.13198428902864917 0.2623603248823557 3 0.037720056019053955 0.11247072918192016 4 0.012576772344315845 0.0500005887444663 5 0.0 0.0 6 0.008389076587237882 0.05002779213546264 7 0.0017981481541971692 0.012510349382325424 8 0.0031476149745174316 0.025027502694345897 9 0.0 0.0 >10 9.00786416684802E-4 0.012534176080292178 >50 0.0 0.0 >100 0.0 0.0 >500 8.189159721358863E-5 0.06152691451987682 >1k 1.5360385101457905E-4 0.21835445425473676 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.001414431389061386 0.0 0.0 2 0.00145523229451508 0.0 0.0 3 0.0015028333508777226 0.0 0.0 4 0.0015504344072403654 0.0 0.0 5 0.0016048356145119571 0.0 0.0 6 0.0016796372745103959 0.0 0.0 7 0.0017408386326909367 0.0 0.0 8 0.001883641801778865 0.0 0.0 9 0.0019448431599594059 0.0 0.0 10-11 0.002091046404501809 0.0 0.0 12-13 0.0022712504035889564 0.0 0.0 14-15 0.0023970531954045126 0.0 0.0 16-17 0.002519455911765594 0.0 0.0 18-19 0.0026826595335803696 0.0 0.0 20-21 0.0029920663999375473 0.0 0.0 22-23 0.003145069795388899 0.0 0.0 24-25 0.003325273794476047 0.0 0.0 26-27 0.0034782771899273986 0.0 0.0 28-29 0.003655081113560072 0.0 0.0 30-31 0.003974688206280674 0.0 0.0 32-33 0.004369096958999714 0.0 0.0 34-35 0.00466150344808452 0.0 0.0 36-37 0.004950509861714851 0.0 0.0 38-39 0.005423120349886805 0.0 0.0 40-41 0.005963732347148248 0.0 0.0 42-43 0.0066335472116797215 0.0 0.0 44-45 0.007357763283482787 0.0 0.0 46-47 0.008119380185285071 0.0 0.0 48-49 0.008996599652539489 0.0 0.0 50-51 0.009986021609791566 0.0 0.0 52-53 0.01114204726431289 0.0 0.0 54-55 0.012525877974284005 0.0 0.0 56-57 0.013794106118802989 0.0 0.0 58-59 0.01530373962058966 0.0 0.0 60-61 0.017098979460552187 0.0 0.0 62-63 0.019166225336872678 0.0 0.0 64-65 0.021240271364102115 0.0 0.0 66-67 0.023531922220417917 0.0 0.0 68-69 0.025969776321276124 0.0 0.0 70-71 0.028618435100311746 0.0 0.0 72-73 0.03165470248115746 0.0 0.0 74-75 0.034925575068361914 0.0 0.0 76-77 0.03837325157919905 0.0 0.0 78-79 0.04179372748640038 0.0 0.0 80-81 0.045931619314495825 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCATTCC 710 0.0 61.316387 3 CATTCCT 835 0.0 51.091747 4 TGAACCG 720 0.0 27.173147 12-13 CTGAACC 780 0.0 24.804207 12-13 GATGGCC 365 0.0 22.661865 3 CCGCCAT 530 0.0 16.40965 8 GCCCTGT 205 6.5257784E-4 14.848733 8 TGGCCGC 700 0.0 14.300864 5 GAACCGT 320 0.0 14.265099 14-15 GGATGGC 620 1.8189894E-12 13.341624 2 CCGTATC 195 5.4434888E-5 11.153403 48-49 CCCTGTC 315 0.0012946912 11.043842 9 CGTTCAT 330 0.0018910151 10.553274 4 AACCGCT 455 2.382876E-10 9.554853 14-15 CCACCCG 400 2.6029738E-8 9.24452 70-71 CTGTCTA 195 0.0075874114 8.920011 10-11 ATCTCAG 200 0.009308131 8.700146 46-47 GCGCCCT 515 8.9311035E-4 8.44533 6 GAGAGTC 390 1.6707054E-6 8.362511 20-21 GTATCAT 295 3.807381E-4 8.110471 50-51 >>END_MODULE