FastQCFastQC Report
Wed 5 Nov 2014
PhiX_Undetermined_L004_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePhiX_Undetermined_L004_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14705556
Sequences flagged as poor quality0
Sequence length93
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTCC7100.061.3163873
CATTCCT8350.051.0917474
TGAACCG7200.027.17314712-13
CTGAACC7800.024.80420712-13
GATGGCC3650.022.6618653
CCGCCAT5300.016.409658
GCCCTGT2056.5257784E-414.8487338
TGGCCGC7000.014.3008645
GAACCGT3200.014.26509914-15
GGATGGC6201.8189894E-1213.3416242
CCGTATC1955.4434888E-511.15340348-49
CCCTGTC3150.001294691211.0438429
CGTTCAT3300.001891015110.5532744
AACCGCT4552.382876E-109.55485314-15
CCACCCG4002.6029738E-89.2445270-71
CTGTCTA1950.00758741148.92001110-11
ATCTCAG2000.0093081318.70014646-47
GCGCCCT5158.9311035E-48.445336
GAGAGTC3901.6707054E-68.36251120-21
GTATCAT2953.807381E-48.11047150-51
Produced by FastQC (version 0.11.2)