FastQCFastQC Report
Wed 5 Nov 2014
PhiX_Undetermined_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePhiX_Undetermined_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14705556
Sequences flagged as poor quality0
Sequence length93
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTCC6250.041.656923
CATTCCT6850.037.3746724
ATGCCGT9850.020.705114
CTGAACC4850.018.34146312-13
CTATCTC1057.8394666E-414.493752536-37
TGAACCT2301.1146767E-813.20663212-13
TGAACCG4450.013.16423412-13
CTTGAAA10550.010.28275316-17
GCTTGAA11100.09.186851516-17
GAACCGT2852.7039127E-59.13541414-15
TCACTGG2250.00245564018.71092780-81
CTGTTAG2350.00362619668.34363874-75
TTGAAAA14400.08.1374618-19
GTACCGC2500.0063506497.83983480-81
ACAGACT3351.7056585E-47.80358776-77
CCCCGTT2550.0076194237.68199648-49
TGTGTCC2600.0090686587.533738686-87
CCCCACG3654.6288257E-47.155832348-49
CCCCCCA37050.06.22118846-47
AAATTCC4400.00387150775.9300428-29
Produced by FastQC (version 0.11.2)