FastQCFastQC Report
Wed 5 Nov 2014
PakO_AGGCAGAA-TAGATCGC_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePakO_AGGCAGAA-TAGATCGC_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40249816
Sequences flagged as poor quality0
Sequence length93
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1086810.27001614119180073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG169950.015.9030595
GTGTAGG140150.014.5029291
CTATACT196450.014.1572744
GTATTAG178250.013.9668471
GTATAAG172500.013.751161
TATACAG167700.013.6249995
TCTATAC209150.013.3184273
GTATAGG133200.013.2337431
CAGCGCG71700.013.1044389
GTATATA329650.013.07115651
GTATAAT281600.012.9521911
GTACTAG148350.012.8797921
TTATACT287800.012.7789964
TATACCG60650.012.77319055
TACACTG132500.012.7117495
ACAGCGC42700.012.4289128
TAGACAG122950.012.3185885
TACACCG71100.012.3037275
CTTACAC127350.012.26948453
GTATAGA207900.012.2261661
Produced by FastQC (version 0.11.2)