FastQCFastQC Report
Wed 5 Nov 2014
PakO_AGGCAGAA-TAGATCGC_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePakO_AGGCAGAA-TAGATCGC_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40249816
Sequences flagged as poor quality0
Sequence length93
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG164750.015.3052175
GTGTAGG137450.014.6157461
GTATTAG171900.014.5259151
TATACAG168000.013.8187535
TATACCG60800.013.6573575
TCTATAC208250.013.6321733
GTATAAG176600.013.30034351
GTATATA339300.013.0489221
TACACTG131600.012.8838485
TAATACT251400.012.8487374
GTATAGG136750.012.8104241
TACACAG118450.012.7726535
TTATACT289950.012.7447914
GTACTAG154400.012.7289921
CTATACT194950.012.6443574
TAGACAG125400.012.4114475
CTTACAC127500.012.3434133
ATTATAC255950.012.2806143
GTATAGA210050.012.1988651
TATACGG58250.012.01619155
Produced by FastQC (version 0.11.2)