FastQCFastQC Report
Wed 5 Nov 2014
PakG_TAGGCATG-TATCCTCT_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePakG_TAGGCATG-TATCCTCT_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33806615
Sequences flagged as poor quality0
Sequence length93
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN949210.2807764101788955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG140000.016.5391795
GTGTAGG118400.014.74159051
CTTACAC102100.013.599283
TATACAG138750.013.5826745
CTATACT171300.013.5685964
TACACCG62000.013.4082495
ACTGTGC95100.013.2652788
GTATAAG147950.013.2388171
TACACAG91450.013.2309635
GTATATA282350.013.1650261
TACACTG107200.013.154695
TATACCG51900.012.9146825
GTATTAG149000.012.9118251
TTATACT241900.012.8833314
GTATAGG115300.012.8351591
TCTATAC177250.012.6465473
GTACTAG127550.012.4214581
GTATAGA180950.012.363921
GACTGTG79900.011.97851857
CTAGGAC118900.011.9706283
Produced by FastQC (version 0.11.2)