FastQCFastQC Report
Wed 5 Nov 2014
PakG_TAGGCATG-TATCCTCT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePakG_TAGGCATG-TATCCTCT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33806615
Sequences flagged as poor quality0
Sequence length93
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG136800.015.9852085
GTGTAGG109800.015.1609711
TACACCG60200.014.1545475
CTATACT166300.013.964
TATACCG52350.013.61957555
TACACTG106300.013.578175
TATACAG137800.013.5030215
TCTATAC176200.013.1262953
GTACTAG124150.013.0926671
GTATTAG142150.013.05956651
CCTACAC116200.012.8703023
TTATACT246150.012.8578394
GTATATA281900.012.76882651
GTATAGG119950.012.6791791
GTATAAG149950.012.5836731
CTTACAC105950.012.4330623
CCTATAC119600.012.3226743
TAGACAG100350.012.3037475
GTATAGA175600.012.2593781
TACGCAG28150.012.200715
Produced by FastQC (version 0.11.2)