##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	PakC_GGACTCCT-CTCTCTAT_L001_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	37507618
Sequences flagged as poor quality	0
Sequence length	93
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26502621414135	33.0	33.0	33.0	33.0	33.0
2	32.25739709730434	33.0	33.0	33.0	33.0	33.0
3	32.27650438905504	33.0	33.0	33.0	33.0	33.0
4	36.105829807694	37.0	37.0	37.0	37.0	37.0
5	36.06548261742454	37.0	37.0	37.0	37.0	37.0
6	36.130553291867265	37.0	37.0	37.0	37.0	37.0
7	36.116977036504956	37.0	37.0	37.0	37.0	37.0
8	36.08339681821437	37.0	37.0	37.0	37.0	37.0
9	36.12372526562471	37.0	37.0	37.0	37.0	37.0
10-11	36.132632976053024	37.0	37.0	37.0	37.0	37.0
12-13	36.12523122636047	37.0	37.0	37.0	37.0	37.0
14-15	38.22821941132065	40.0	37.0	40.0	37.0	40.0
16-17	38.14095828479431	40.0	37.0	40.0	37.0	40.0
18-19	38.17378592263577	40.0	37.0	40.0	37.0	40.0
20-21	38.119392932390426	40.0	37.0	40.0	37.0	40.0
22-23	38.05320344523078	40.0	37.0	40.0	37.0	40.0
24-25	37.987662599635094	40.0	37.0	40.0	33.0	40.0
26-27	37.91879008419036	40.0	37.0	40.0	33.0	40.0
28-29	37.827233097020454	40.0	37.0	40.0	33.0	40.0
30-31	37.7249571273761	40.0	37.0	40.0	33.0	40.0
32-33	37.635148625007325	40.0	37.0	40.0	33.0	40.0
34-35	37.46473011162692	40.0	37.0	40.0	33.0	40.0
36-37	37.3766184912089	40.0	37.0	40.0	33.0	40.0
38-39	37.29182429020153	40.0	37.0	40.0	33.0	40.0
40-41	37.25429957988801	40.0	37.0	40.0	33.0	40.0
42-43	37.26307772996942	40.0	37.0	40.0	33.0	40.0
44-45	37.17431684144805	40.0	37.0	40.0	33.0	40.0
46-47	37.0225677487704	40.0	37.0	40.0	33.0	40.0
48-49	36.87806207794907	40.0	37.0	40.0	33.0	40.0
50-51	35.72544613470255	38.5	35.0	38.5	30.0	40.0
52-53	35.785116186263814	37.0	37.0	38.5	33.0	40.0
54-55	36.45610581029166	37.0	37.0	40.0	33.0	40.0
56-57	36.485030321040384	37.0	37.0	40.0	33.0	40.0
58-59	36.36193336777612	37.0	37.0	40.0	33.0	40.0
60-61	36.58167295507809	37.0	37.0	40.0	33.0	40.0
62-63	36.53287865414434	37.0	37.0	40.0	33.0	40.0
64-65	36.36563496247616	37.0	37.0	40.0	33.0	40.0
66-67	36.15571348732409	37.0	37.0	40.0	33.0	40.0
68-69	35.93469052073635	37.0	37.0	40.0	33.0	40.0
70-71	35.711996227006466	37.0	37.0	40.0	33.0	40.0
72-73	35.47390411462546	37.0	35.0	40.0	27.0	40.0
74-75	35.20537565195423	37.0	33.0	37.0	27.0	40.0
76-77	34.9623180949534	37.0	33.0	37.0	27.0	40.0
78-79	34.699049350454615	37.0	33.0	37.0	27.0	40.0
80-81	34.41806780425246	37.0	33.0	37.0	27.0	37.0
82-83	34.15520393750411	37.0	33.0	37.0	27.0	37.0
84-85	33.95785006928459	37.0	33.0	37.0	27.0	37.0
86-87	33.75516784350315	37.0	33.0	37.0	27.0	37.0
88-89	33.583482867400434	37.0	33.0	37.0	27.0	37.0
90-91	33.39539687644255	37.0	33.0	37.0	27.0	37.0
92-93	32.463777451823255	35.0	33.0	37.0	24.5	37.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
2304	1	0.07304912440258704
2304	2	0.05954773881692432
2304	3	0.10399557641960655
2304	4	0.06365016419461256
2304	5	0.040506977601744154
2304	6	0.10201187114162735
2304	7	0.13342026953125696
2304	8	0.23470545976811508
2304	9	0.25887078171787437
2304	10-11	0.2651621547766396
2304	12-13	0.27608672074862994
2304	14-15	0.4569849781880677
2304	16-17	0.48036269005669396
2304	18-19	0.4368783954345119
2304	20-21	0.42266642480323213
2304	22-23	0.4192970703849781
2304	24-25	0.4994998380306299
2304	26-27	0.49559351973730514
2304	28-29	0.46768033010204135
2304	30-31	0.4654630595555673
2304	32-33	0.40455937242641227
2304	34-35	0.5207180174970532
2304	36-37	0.5446465175099675
2304	38-39	0.5115645662087047
2304	40-41	0.43658327755803583
2304	42-43	0.4647464300286046
2304	44-45	0.4809661579908848
2304	46-47	0.3888144042888797
2304	48-49	0.43317285186422794
2304	50-51	0.35667680240155164
2304	52-53	0.4505491844732461
2304	54-55	0.6108749345474038
2304	56-57	0.5628490745348884
2304	58-59	0.5173911171045305
2304	60-61	0.5100042776273952
2304	62-63	0.5267804765570006
2304	64-65	0.5040395438248098
2304	66-67	0.49813121884464806
2304	68-69	0.6129447364321905
2304	70-71	0.574293691432004
2304	72-73	0.5089499743465637
2304	74-75	0.5091609417745673
2304	76-77	0.4702914840459371
2304	78-79	0.48911474217358375
2304	80-81	0.4891432973471126
2304	82-83	0.5103905625939191
2304	84-85	0.4233782906783219
2304	86-87	0.42988023904686656
2304	88-89	0.43837538965081535
2304	90-91	0.3971647117961581
2304	92-93	0.42652427479273314
2305	1	0.10931137577919259
2305	2	0.1760952889669838
2305	3	0.17442040955214821
2305	4	0.140544248164872
2305	5	0.10348761081257862
2305	6	0.1403575084424702
2305	7	0.16335396847215122
2305	8	0.24049970909014462
2305	9	0.2965275346998908
2305	10-11	0.3312616041587617
2305	12-13	0.3635191714281376
2305	14-15	0.5652932129326942
2305	16-17	0.6294535612158967
2305	18-19	0.5402664135605733
2305	20-21	0.49769547828083915
2305	22-23	0.5301935860124871
2305	24-25	0.5133569753356042
2305	26-27	0.5830223563315613
2305	28-29	0.5667179287524462
2305	30-31	0.5180032711927964
2305	32-33	0.49650087360144113
2305	34-35	0.6241717590817686
2305	36-37	0.5405732307435045
2305	38-39	0.5898141241245085
2305	40-41	0.559514951981896
2305	42-43	0.6066618539495252
2305	44-45	0.6469737879406168
2305	46-47	0.6354768953342145
2305	48-49	0.5358499084458188
2305	50-51	0.509813616111991
2305	52-53	0.5490292126932275
2305	54-55	0.7347853593368825
2305	56-57	0.7732166504245228
2305	58-59	0.733792010431209
2305	60-61	0.7003728208842617
2305	62-63	0.6856418284493628
2305	64-65	0.6530890182940396
2305	66-67	0.7235343540432879
2305	68-69	0.7031016641241976
2305	70-71	0.5937510740901146
2305	72-73	0.6380118813646476
2305	74-75	0.6326473277018252
2305	76-77	0.5775134754766853
2305	78-79	0.6051410770258556
2305	80-81	0.5621631926514397
2305	82-83	0.5196054699236967
2305	84-85	0.5936172027432249
2305	86-87	0.577996870875225
2305	88-89	0.5103289537614017
2305	90-91	0.4398777357350596
2305	92-93	0.4751558838977914
2306	1	0.09394009214790344
2306	2	0.1183991560862907
2306	3	0.18691501885895434
2306	4	0.178661901637021
2306	5	0.1358305222940146
2306	6	0.1401577836058152
2306	7	0.17076919248295042
2306	8	0.25989484420820474
2306	9	0.29074958274817675
2306	10-11	0.27838054419184033
2306	12-13	0.2921846805647519
2306	14-15	0.4490935541261578
2306	16-17	0.5470308307467349
2306	18-19	0.4593350909198435
2306	20-21	0.4210755651489748
2306	22-23	0.435687567892991
2306	24-25	0.4891696977059681
2306	26-27	0.4764103970076192
2306	28-29	0.5084342231412791
2306	30-31	0.5042401182244021
2306	32-33	0.48636229741449455
2306	34-35	0.5943930741545032
2306	36-37	0.47045310326848266
2306	38-39	0.41127916066155024
2306	40-41	0.5330046525953733
2306	42-43	0.5454796273795708
2306	44-45	0.49888826353512883
2306	46-47	0.5179200365190724
2306	48-49	0.4727868388913734
2306	50-51	0.502980806598579
2306	52-53	0.6002717315295385
2306	54-55	0.7486383742333231
2306	56-57	0.7201200350796029
2306	58-59	0.6649364658448249
2306	60-61	0.6399390704004517
2306	62-63	0.6001715988037475
2306	64-65	0.5753988880699481
2306	66-67	0.6343814479127019
2306	68-69	0.5999875058403887
2306	70-71	0.6141985138616164
2306	72-73	0.609905119538233
2306	74-75	0.6081089085401459
2306	76-77	0.555802921218671
2306	78-79	0.5859639682470288
2306	80-81	0.6617891620598471
2306	82-83	0.6259919686151036
2306	84-85	0.543826855351405
2306	86-87	0.5351775983018427
2306	88-89	0.3907568278313249
2306	90-91	0.42828589547659845
2306	92-93	0.561491211322199
2307	1	0.03239912995021399
2307	2	0.08065189106328319
2307	3	0.08662071357285583
2307	4	0.05307166868104218
2307	5	-0.032506628263874404
2307	6	0.028849454975734545
2307	7	0.06778658646737057
2307	8	0.12407301220071787
2307	9	0.15956415499491072
2307	10-11	0.17744694526093951
2307	12-13	0.19584814170257658
2307	14-15	0.33568735701597774
2307	16-17	0.4276984783592468
2307	18-19	0.33062493492230516
2307	20-21	0.3201114979371127
2307	22-23	0.3134773730347362
2307	24-25	0.34145677499012805
2307	26-27	0.36031517662613055
2307	28-29	0.364656465292029
2307	30-31	0.3465304872743715
2307	32-33	0.3628669120004915
2307	34-35	0.4245918583681245
2307	36-37	0.40871657330428235
2307	38-39	0.42077536338263144
2307	40-41	0.36695158883066625
2307	42-43	0.3617339131637962
2307	44-45	0.347916264412369
2307	46-47	0.4198754477551887
2307	48-49	0.4344355658903041
2307	50-51	0.3097990607519918
2307	52-53	0.39555839387523406
2307	54-55	0.5319587374566055
2307	56-57	0.5080058521203128
2307	58-59	0.43988608232326243
2307	60-61	0.39282122797818175
2307	62-63	0.429286131078328
2307	64-65	0.4504296322575314
2307	66-67	0.4703582432139157
2307	68-69	0.42035203682872435
2307	70-71	0.44278084653077343
2307	72-73	0.4290988302837704
2307	74-75	0.44528587024578314
2307	76-77	0.40097549110640074
2307	78-79	0.4354309813087909
2307	80-81	0.38118323457744197
2307	82-83	0.3946869909675499
2307	84-85	0.43630034496499803
2307	86-87	0.4107430696703034
2307	88-89	0.4536194306401953
2307	90-91	0.4404737078252694
2307	92-93	0.45967318553558556
2308	1	0.1447112899878107
2308	2	0.13449479628106786
2308	3	0.1459551916590769
2308	4	0.1486456902809934
2308	5	0.10892688989721222
2308	6	0.077807054352526
2308	7	0.14874268798958923
2308	8	0.23097567867957025
2308	9	0.2833646928715936
2308	10-11	0.2667530431352034
2308	12-13	0.20600438233487495
2308	14-15	0.38731765910065263
2308	16-17	0.5037199141463873
2308	18-19	0.3691814133230835
2308	20-21	0.3884588843917314
2308	22-23	0.39209745473316104
2308	24-25	0.38002781783291795
2308	26-27	0.40256994792267875
2308	28-29	0.43981100583322075
2308	30-31	0.39797165737717677
2308	32-33	0.3894133468953953
2308	34-35	0.5236736597314646
2308	36-37	0.49985438656425885
2308	38-39	0.4824197981834004
2308	40-41	0.4774395783693208
2308	42-43	0.4855390479784134
2308	44-45	0.49259977516162223
2308	46-47	0.49804961095530587
2308	48-49	0.4462485985914597
2308	50-51	0.45515873348137603
2308	52-53	0.4741153742951951
2308	54-55	0.612415082049246
2308	56-57	0.6228161841106541
2308	58-59	0.6163267881629295
2308	60-61	0.57108372653083
2308	62-63	0.5534743515261411
2308	64-65	0.5734957250965209
2308	66-67	0.5942109107576314
2308	68-69	0.5757485976342664
2308	70-71	0.5867603211189802
2308	72-73	0.568779086776857
2308	74-75	0.5209041123885072
2308	76-77	0.5200594189242693
2308	78-79	0.5262858432104238
2308	80-81	0.559955062702997
2308	82-83	0.5689199101707274
2308	84-85	0.5832160294570983
2308	86-87	0.5673917299237843
2308	88-89	0.5643391649362144
2308	90-91	0.6405320191610784
2308	92-93	0.5671422582217147
2309	1	0.11884792247142428
2309	2	0.12453115391591751
2309	3	0.1524945160666391
2309	4	0.09391613031929325
2309	5	0.11772532442647332
2309	6	0.10640978351613484
2309	7	0.169980973692077
2309	8	0.22098414719024362
2309	9	0.22022411102577166
2309	10-11	0.2362610200937283
2309	12-13	0.2551882218773045
2309	14-15	0.3689295133661119
2309	16-17	0.48934861410097596
2309	18-19	0.3967884916471647
2309	20-21	0.42928990795916633
2309	22-23	0.37549390819500417
2309	24-25	0.36733206679340213
2309	26-27	0.39320521547058007
2309	28-29	0.4281198313740404
2309	30-31	0.467393269734778
2309	32-33	0.4461662343073556
2309	34-35	0.5420813752471574
2309	36-37	0.46356741930431866
2309	38-39	0.31888740368664514
2309	40-41	0.38226547615766293
2309	42-43	0.32896541216634745
2309	44-45	0.35643913991171416
2309	46-47	0.37487867445055656
2309	48-49	0.37247538206103314
2309	50-51	0.3962369252008884
2309	52-53	0.41237337340508873
2309	54-55	0.5597198966360537
2309	56-57	0.5492225736020302
2309	58-59	0.4513341588193853
2309	60-61	0.44751001254415
2309	62-63	0.4448854688247721
2309	64-65	0.4114978817719859
2309	66-67	0.39828422180799095
2309	68-69	0.440412204955102
2309	70-71	0.4564508253637243
2309	72-73	0.4823751843442565
2309	74-75	0.35478156890555823
2309	76-77	0.3904013692753594
2309	78-79	0.44351752782802834
2309	80-81	0.5220820045981327
2309	82-83	0.44983411671684337
2309	84-85	0.44753193077016107
2309	86-87	0.4024910329683493
2309	88-89	0.4032671301203692
2309	90-91	0.5215787132756589
2309	92-93	0.590494392801169
2310	1	0.09545683508584801
2310	2	0.13947880444460026
2310	3	0.16055316296340294
2310	4	0.06890469279068867
2310	5	0.13907678610734564
2310	6	0.1075877903107667
2310	7	0.15220736109188238
2310	8	0.25169131013082335
2310	9	0.17749081623735918
2310	10-11	0.2004213740365799
2310	12-13	0.22815217293337042
2310	14-15	0.3807158461232092
2310	16-17	0.4740678060170893
2310	18-19	0.35633084347026767
2310	20-21	0.3473044353751433
2310	22-23	0.3144065000371583
2310	24-25	0.32230841183068293
2310	26-27	0.3373579314477837
2310	28-29	0.35614520370600644
2310	30-31	0.3621971814746203
2310	32-33	0.36566296669008125
2310	34-35	0.5190191754910174
2310	36-37	0.4678245793901894
2310	38-39	0.3984520913651579
2310	40-41	0.47216619901182355
2310	42-43	0.4707475738848004
2310	44-45	0.44817996372341184
2310	46-47	0.44850660435346157
2310	48-49	0.46866210666977537
2310	50-51	0.40703798066503083
2310	52-53	0.4481735850960362
2310	54-55	0.6180860092903799
2310	56-57	0.5731428878728835
2310	58-59	0.5318249046515504
2310	60-61	0.48796860110387286
2310	62-63	0.5160824924621821
2310	64-65	0.5132048970087837
2310	66-67	0.4927072451968755
2310	68-69	0.48919697491839287
2310	70-71	0.5364652134169248
2310	72-73	0.5444249229846818
2310	74-75	0.5301983934720482
2310	76-77	0.5240790884328419
2310	78-79	0.5604594761397124
2310	80-81	0.5657848879593104
2310	82-83	0.5750267988320346
2310	84-85	0.5572890834186879
2310	86-87	0.5211629403885212
2310	88-89	0.5280443995902147
2310	90-91	0.5111897183839105
2310	92-93	0.6256524626080093
2311	1	0.1362101787560519
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1304	1	0.06334314936805896
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1304	4	0.045972878803567596
1304	5	0.11695021577169484
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1304	7	0.15007504585659603
1304	8	0.23386222069772344
1304	9	0.2798177264777024
1304	10-11	0.26556948587377605
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1304	16-17	0.5568197615489936
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1304	24-25	0.4284849997165381
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1305	1	0.1482702414086461
1305	2	0.16660642408984927
1305	3	0.20495214612686397
1305	4	0.1445776593627528
1305	5	0.1832282934788978
1305	6	0.13504981650274317
1305	7	0.21041155690502222
1305	8	0.3301894167287429
1305	9	0.3339822767176841
1305	10-11	0.3901897035068842
1305	12-13	0.3860646731585575
1305	14-15	0.5874675162569005
1305	16-17	0.6516217612193671
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1305	20-21	0.6307633172957665
1305	22-23	0.5944295167223856
1305	24-25	0.5840990739931726
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1305	28-29	0.5426183502501658
1305	30-31	0.46722822117373397
1305	32-33	0.5163244192833574
1305	34-35	0.6325109224338732
1305	36-37	0.5459509389979331
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1305	56-57	0.39725226304860684
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1305	60-61	0.37670619834921837
1305	62-63	0.3778974660343195
1305	64-65	0.3836683194246362
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1305	70-71	0.28669806639296525
1305	72-73	0.3071540085540576
1305	74-75	0.29518670672062797
1305	76-77	0.24269870533153437
1305	78-79	0.17280841372234335
1305	80-81	0.2297523704645812
1305	82-83	0.22785710106360568
1305	84-85	0.11908529687487857
1305	86-87	0.1723510475950647
1305	88-89	0.04146125244899679
1305	90-91	-0.08588419438800088
1305	92-93	0.007400321083927963
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1306	1	0.1684824472224662
1306	2	0.21749876975410132
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1306	9	0.2410578308257385
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1306	12-13	0.22326602918709426
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1306	78-79	-0.0028325807665368075
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1306	84-85	0.016565710303169112
1306	86-87	-0.03453045886490713
1306	88-89	-0.16940334340621632
1306	90-91	-0.11326419696495549
1306	92-93	-0.08844703890364514
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1307	88-89	0.4544475669114192
1307	90-91	0.4310874853494582
1307	92-93	0.44141294867090863
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1308	2	0.12003395008731843
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2204	26-27	-0.03272762421693898
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2204	30-31	-0.05838374899423826
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2204	54-55	-0.0413822046048935
2204	56-57	-0.082496356981963
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1310	1	0.03598087784735071
1310	2	0.031002860749140382
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1310	88-89	-0.39007569686414456
1310	90-91	-0.30868617626593675
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1311	10-11	-0.3373299822747313
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1311	22-23	-0.7096251039020416
1311	24-25	-0.7373834907243761
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1311	28-29	-0.7540121349684341
1311	30-31	-0.7138946961241999
1311	32-33	-0.8078787027303989
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1311	88-89	-0.8532228957659953
1311	90-91	-0.8831502623633369
1311	92-93	-1.0970395341144936
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1313	1	0.16271439125269893
1313	2	0.2097510870878665
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1313	5	0.2009349567176173
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1313	26-27	0.5744539059121436
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2303	12-13	-0.34520722960620986
2303	14-15	-0.6844215067329884
2303	16-17	-0.7433359477097952
2303	18-19	-0.7431733785084731
2303	20-21	-0.551057591789494
2303	22-23	-0.5758784808173587
2303	24-25	-0.5825002785752034
2303	26-27	-0.7571706837161898
2303	28-29	-0.4771638695172413
2303	30-31	-0.7532289150536258
2303	32-33	-0.4813492539605093
2303	34-35	-0.3843928412064841
2303	36-37	-0.3722313000993509
2303	38-39	-0.37587598903536446
2303	40-41	-0.2994635899188225
2303	42-43	-0.6701571221596296
2303	44-45	-0.4358458632135367
2303	46-47	-0.808517483735443
2303	48-49	-0.3447184012971647
2303	50-51	-0.1629130066717721
2303	52-53	-0.09262748751190486
2303	54-55	-0.09205909937752921
2303	56-57	-0.1717849013288273
2303	58-59	-0.1230378077257015
2303	60-61	-0.21602024036186407
2303	62-63	-0.3284859618487914
2303	64-65	-0.30200433975678465
2303	66-67	-0.7646864470795194
2303	68-69	-0.3879932903548635
2303	70-71	-0.28110951913587456
2303	72-73	-0.3453443959253022
2303	74-75	-0.2328204593309806
2303	76-77	-0.2007990439822791
2303	78-79	-0.1694232668791784
2303	80-81	-0.199012146106611
2303	82-83	-0.1408083944705254
2303	84-85	-0.005459628414051565
2303	86-87	-0.10793129898380727
2303	88-89	-0.09786649673692693
2303	90-91	-0.10043244684592878
2303	92-93	-0.21849003083717378
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	110665.0
3	3841.0
4	2626.0
5	1946.0
6	1765.0
7	3089.0
8	6580.0
9	8511.0
10	11151.0
11	16625.0
12	24716.0
13	32845.0
14	39976.0
15	46647.0
16	53549.0
17	61780.0
18	70806.0
19	81015.0
20	92594.0
21	105147.0
22	120987.0
23	138754.0
24	160634.0
25	186860.0
26	220594.0
27	260569.0
28	313696.0
29	383433.0
30	478663.0
31	615853.0
32	817896.0
33	1119751.0
34	1607880.0
35	2472462.0
36	4311926.0
37	9154341.0
38	1.3681049E7
39	686396.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.86161511737128	23.337406326203062	11.067414524809145	24.733564031616513
2	15.774034818052982	22.533169097907578	38.58854316077018	23.104252923269268
3	15.508945833240114	27.179492133706173	31.6630703428311	25.648491690222606
4	11.499209543229846	16.97522307593112	42.275048229559374	29.250519151279665
5	12.008316607064137	38.16598097661784	38.0251136266611	11.800588789656915
6	28.51573986072524	41.680722857232226	18.412340866605923	11.391196415436603
7	25.980296353835293	32.7083217789374	26.18287785536714	15.128504011860166
8	23.22927058875651	37.37980842223163	22.93298470043003	16.457936288581834
9	23.82663352820519	15.047233971936539	24.59041422889579	36.535718270962484
10-11	22.591127855129024	26.854723887551142	31.727937975264098	18.826210282055737
12-13	22.118526248587504	25.230330081905883	31.501227331731346	21.14991633777527
14-15	20.157301412584232	26.76621180210004	27.09390769673594	25.982579088579783
16-17	20.25860789870842	31.245747926725414	28.149169400325825	20.346474774240342
18-19	20.27831064430084	29.9803813948418	29.763128223153036	19.978179737704323
20-21	20.57851275592754	29.56714995319248	29.490516553224992	20.36382073765499
22-23	20.656829266064605	29.375522819860162	29.474076407365224	20.49357150671001
24-25	20.20715315801841	29.26982162911036	29.78536941702432	20.73765579584691
26-27	20.493933100931514	29.84750527765024	29.45556741033511	20.20299421108314
28-29	20.524956579437536	29.667337348616456	29.45647453705239	20.351231534893614
30-31	20.291207425459763	29.512600672671326	29.63820805872002	20.557983843148886
32-33	20.500129129808204	29.77990045214626	29.497469372748796	20.222501045296738
34-35	20.439610420676722	29.468750519532765	29.602565441601215	20.489073618189295
36-37	20.35166912751869	29.697090692811045	29.65110027565493	20.300139904015335
38-39	20.39671245409303	29.64479216093045	29.65727945845576	20.30121592652076
40-41	20.278466183958884	29.554883510785874	29.81990359746706	20.34674670778818
42-43	20.25283952530087	29.599986314606074	29.825121209763143	20.322052950329912
44-45	20.231652041818464	29.435370703378737	29.804155559778216	20.528821695024586
46-47	20.182633209891293	29.449047000231076	29.974462601245545	20.39385718863209
48-49	20.198540558768894	29.6428534509803	29.85394875992455	20.304657230326253
50-51	20.07484786907322	29.598261335621572	29.956585673918244	20.370305121386963
52-53	20.168404012384507	29.446486938591786	30.071687773895494	20.313421275128213
54-55	20.19285395755621	29.5603547108784	29.94143082953728	20.30536050202811
56-57	20.07208218835903	29.55852424902361	29.917916019129294	20.45147754348807
58-59	20.142017117062636	29.5925883017929	29.830106135494137	20.435288445650322
60-61	19.98612264503049	29.518493343875114	30.008441130166524	20.48694288092787
62-63	19.984286683319247	29.55419332636283	29.93189531753489	20.52962467278303
64-65	20.09342615035914	29.451585902146682	29.976887602741275	20.478100344752903
66-67	20.050908862189058	29.39407640139059	29.896750817523632	20.658263918896722
68-69	20.068901695971267	29.558053109762184	29.778412268814257	20.594632925452288
70-71	20.155953285651414	29.503010751370795	29.792207450360742	20.54882851261705
72-73	20.140629077618314	29.52167374870302	29.70342427595407	20.634272897724596
74-75	20.1020901661019	29.616562257829983	29.731808737203387	20.549538838864738
76-77	20.181001091140665	29.608383167332025	29.671496619726394	20.539119121800915
78-79	20.33923424996018	29.56199843159234	29.610279862064747	20.48848745638273
80-81	20.289275509881215	29.67119203465622	29.534794966559964	20.504737488902602
82-83	20.377748211721517	29.66901314078204	29.57630366505718	20.376934982439263
84-85	20.367288178728156	29.704938988945916	29.550133334592417	20.37763949773351
86-87	20.304284951857195	29.549497949932462	29.624442254645476	20.521774843564867
88-89	20.438316013837763	29.54130046403059	29.52525660773812	20.495126914393527
90-91	20.564238093501107	29.644460233673907	29.459439731418303	20.331861941406686
92-93	20.717866299171753	29.514289878391864	29.387202107696446	20.380641714739937
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	104755.0
1	54349.5
2	3289.5
3	2404.0
4	1999.0
5	1746.5
6	1691.5
7	1757.5
8	1963.5
9	2399.5
10	3111.5
11	4394.5
12	6460.5
13	9548.5
14	11414.0
15	14141.5
16	20875.5
17	31681.5
18	48322.0
19	72314.5
20	106295.5
21	152767.5
22	214508.0
23	293458.5
24	388619.5
25	499266.0
26	627343.5
27	769682.5
28	919286.0
29	995290.0
30	1063386.0
31	1196848.0
32	1319868.5
33	1426343.0
34	1517837.5
35	1587613.0
36	1625398.5
37	1635882.5
38	1621071.5
39	1585611.5
40	1541904.0
41	1484175.0
42	1418436.5
43	1387569.0
44	1349763.0
45	1257485.5
46	1158669.5
47	1066477.5
48	973114.0
49	884280.5
50	801788.0
51	727077.5
52	655036.5
53	587059.0
54	525754.0
55	471832.0
56	426313.0
57	404883.0
58	386109.0
59	349549.5
60	313032.5
61	279677.5
62	248927.0
63	218597.5
64	192736.0
65	172086.5
66	154503.0
67	138610.5
68	121380.0
69	103026.0
70	85057.5
71	75512.0
72	70644.0
73	60904.0
74	54366.5
75	52032.5
76	50088.5
77	45370.0
78	36940.0
79	31656.5
80	28107.5
81	23459.5
82	19854.0
83	15813.0
84	12022.0
85	8686.5
86	7311.0
87	5919.0
88	3831.5
89	2642.0
90	1814.5
91	1302.5
92	1003.5
93	827.0
94	666.0
95	472.0
96	329.5
97	230.0
98	151.5
99	85.5
100	52.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35451998044770533
2	0.3692529874864354
3	0.3728042660560316
4	0.3883904331114815
5	0.38986213414032317
6	0.3615025619595465
7	0.3467802194210254
8	0.3435435436075946
9	0.3431169635992347
10-11	0.3335669036620774
12-13	0.3281026803674923
14-15	0.3339201652315005
16-17	0.31790741816769064
18-19	0.31353630614452777
20-21	0.32829464137125425
22-23	0.3316726218124542
24-25	0.3246087234865194
26-27	0.31363095358388265
28-29	0.31940711350958095
30-31	0.339271078211365
32-33	0.33244579807760655
34-35	0.3313153610554528
36-37	0.33571180126661204
38-39	0.33494662337661646
40-41	0.341260007500343
42-43	0.33245779564034167
44-45	0.33936305952566753
46-47	0.33585710508195965
48-49	0.31615577400836276
50-51	0.30723758570858856
52-53	0.3033890341956666
54-55	0.31523596086533673
56-57	0.33145000037059136
58-59	0.34099339499511805
60-61	0.3432289408514292
62-63	0.33918976139727136
64-65	0.35079007149960845
66-67	0.34565378158644994
68-69	0.3381326428140545
70-71	0.33261776314347663
72-73	0.31948443113609615
74-75	0.33477199218569414
76-77	0.3056419098648173
78-79	0.3131803784500525
80-81	0.31820469111101646
82-83	0.33536253888476736
84-85	0.3356878061411418
86-87	0.33377486141615287
88-89	0.3351519150056396
90-91	0.3404814989850862
92-93	0.320124301148636
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
93	3.7507618E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.77367749393801
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.13642967915665	64.14012108938093
2	7.686045044130539	11.18683526490873
3	1.8069980349686519	3.945056766869652
4	0.7140400874146219	2.0785329215701998
5	0.3113034565385938	1.132734867444389
6	0.2565962189094463	1.1204070288647978
7	0.20285489070207982	1.033374747581484
8	0.11335423445155193	0.6599363600439583
9	0.0855595129286192	0.560383236036518
>10	0.6191202270859709	8.531315769962946
>50	0.04791604604164419	2.3817763769941216
>100	0.01899475327277642	2.430679011192367
>500	6.575471491442836E-4	0.3067806153253854
>1k	1.0917300871319735E-4	0.10307504473490055
>5k	1.3515517645371161E-5	0.077121503753391
>10k+	7.578723441526623E-6	0.31186939533628777
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	103665	0.2763838535414326	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	2.6661250522493856E-6	5.332250104498771E-6	0.0
2	2.6661250522493856E-6	1.0664500208997542E-5	2.6661250522493856E-6
3	2.6661250522493856E-6	1.0664500208997542E-5	5.332250104498771E-6
4	2.6661250522493856E-6	1.3330625261246929E-5	7.998375156748158E-6
5	1.5996750313496315E-5	1.5996750313496315E-5	7.998375156748158E-6
6	2.1329000417995085E-5	1.5996750313496315E-5	7.998375156748158E-6
7	2.9327375574743244E-5	1.5996750313496315E-5	1.0664500208997542E-5
8	4.532412588823956E-5	1.5996750313496315E-5	1.3330625261246929E-5
9	5.3322501044987715E-5	1.86628753657457E-5	2.9327375574743244E-5
10-11	6.132087620173587E-5	2.266206294411978E-5	7.065231388460872E-5
12-13	9.598050188097788E-5	2.7994313048618552E-5	8.798212672422973E-5
14-15	1.0664500208997543E-4	3.465962567924201E-5	9.997968945935195E-5
16-17	1.1064418966834951E-4	4.532412588823956E-5	1.5996750313496314E-4
18-19	1.266409399818458E-4	5.732168862336179E-5	2.3328594207182126E-4
20-21	1.4397075282146683E-4	6.398700125398526E-5	3.2526725637442507E-4
22-23	1.51969127978215E-4	6.665312630623464E-5	5.478886982372488E-4
24-25	1.5996750313496314E-4	7.198537641073342E-5	0.0012650763372923333
26-27	1.666328157655866E-4	7.59845639891075E-5	0.003194017812594764
28-29	1.7996344102683354E-4	8.131681409360627E-5	0.013402610637657662
30-31	1.9062794123583107E-4	8.664906419810503E-5	0.024717645359404055
32-33	1.9729325386645455E-4	9.598050188097788E-5	0.03942265808508554
34-35	2.0929081660157677E-4	1.0264581451160134E-4	0.06916061691787519
36-37	2.239545043889484E-4	1.0931112714222482E-4	0.14023551162326545
38-39	2.3461900459794592E-4	1.1331031472059889E-4	0.2638810601089091
40-41	2.5594800501594104E-4	1.1997562735122235E-4	0.4076198600508302
42-43	2.759439429078114E-4	1.2264175240347174E-4	0.5568922025386949
44-45	2.9327375574743244E-4	1.2397481492959644E-4	0.7371302544459102
46-47	3.132696936393028E-4	1.3064012756021988E-4	0.9532370197435625
48-49	3.372648191095473E-4	1.3597237766471866E-4	1.1746400424575083
50-51	3.6392606963204117E-4	1.506360654520903E-4	1.3947326647082734
52-53	3.7992281994553745E-4	1.5596831555658906E-4	1.606181709539646
54-55	4.0391794541578196E-4	1.5730137808271375E-4	1.8281846103903479
56-57	4.1858163320315355E-4	1.666328157655866E-4	2.0771047097685598
58-59	4.372445085688993E-4	1.7329812839621007E-4	2.3338165596119698
60-61	4.705710717220166E-4	1.7463119092233476E-4	2.58643857362523
62-63	4.919000721400116E-4	1.8396262860520763E-4	2.827490937974254
64-65	5.065637599273833E-4	1.86628753657457E-4	3.064221513613581
66-67	5.265596978192538E-4	1.86628753657457E-4	3.320074871190167
68-69	5.425564481327499E-4	1.9062794123583107E-4	3.587644515308863
70-71	5.63885448550745E-4	2.03958566497078E-4	3.8511056607220433
72-73	5.758830112858673E-4	2.052916290232027E-4	4.1047821271934675
74-75	5.932128241254884E-4	2.052916290232027E-4	4.347046245378738
76-77	6.065434493867353E-4	2.0662469154932738E-4	4.599247011633743
78-79	6.252063247524809E-4	2.1329000417995086E-4	4.864873050589349
80-81	6.278724498047304E-4	2.1728919175832494E-4	5.133135887221631
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACTG	15600	0.0	15.874537	5
GTATATA	31185	0.0	14.277973	1
GTATTAG	16485	0.0	13.6765785	1
TATACCG	5355	0.0	13.57283	5
GTATAAG	16755	0.0	13.534119	1
GTGTAGG	13125	0.0	13.49683	1
CTATACT	18390	0.0	13.490536	4
TATACAG	14985	0.0	13.331203	5
TACACTG	12125	0.0	13.101605	5
TTATACT	26700	0.0	12.95961	4
GTATAGG	12730	0.0	12.958283	1
ATTATAC	23180	0.0	12.730701	3
GTATAAT	24995	0.0	12.624714	1
TCTATAC	19230	0.0	12.607007	3
TACGCAG	3005	0.0	12.6005125	5
GTATAGA	18970	0.0	12.343878	1
GTATTAT	26585	0.0	12.295322	1
CTTACAC	12045	0.0	12.177529	3
TAGACAG	11380	0.0	12.085322	5
CAGCGCG	5320	0.0	12.019587	9
>>END_MODULE