FastQCFastQC Report
Wed 5 Nov 2014
PakC_GGACTCCT-CTCTCTAT_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePakC_GGACTCCT-CTCTCTAT_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37507618
Sequences flagged as poor quality0
Sequence length93
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1036650.2763838535414326No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG156000.015.8745375
GTATATA311850.014.2779731
GTATTAG164850.013.67657851
TATACCG53550.013.572835
GTATAAG167550.013.5341191
GTGTAGG131250.013.496831
CTATACT183900.013.4905364
TATACAG149850.013.3312035
TACACTG121250.013.1016055
TTATACT267000.012.959614
GTATAGG127300.012.9582831
ATTATAC231800.012.7307013
GTATAAT249950.012.6247141
TCTATAC192300.012.6070073
TACGCAG30050.012.60051255
GTATAGA189700.012.3438781
GTATTAT265850.012.2953221
CTTACAC120450.012.1775293
TAGACAG113800.012.0853225
CAGCGCG53200.012.0195879
Produced by FastQC (version 0.11.2)