FastQCFastQC Report
Wed 5 Nov 2014
PakC_GGACTCCT-CTCTCTAT_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePakC_GGACTCCT-CTCTCTAT_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37507618
Sequences flagged as poor quality0
Sequence length93
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG155350.015.4756115
TATACAG155450.014.5986855
TACACTG120100.014.2984195
TCTATAC196450.014.0746963
GTGTAGG131600.014.0409331
CTTACAC122900.013.5128013
GTATAAG164700.013.3094591
CTATACT183000.013.2561394
GTATTAG163450.013.2246061
GTATAGG126150.012.9893251
ATGCCGT142100.012.86978286-87
TAGACAG114300.012.7418515
ACTGTGC106350.012.7132588
TTATACT277000.012.6499614
TAATACT232650.012.5013514
GTATATA309550.012.4875961
TATGCCG168550.012.37254786-87
ATAATAC258000.012.3514593
TACACAG114000.012.2414865
GTATAGA192050.012.1174971
Produced by FastQC (version 0.11.2)