FastQCFastQC Report
Wed 5 Nov 2014
Manjouma_TAAGGCGA-AGAGTAGA_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameManjouma_TAAGGCGA-AGAGTAGA_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42483910
Sequences flagged as poor quality0
Sequence length93
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1158190.27261850427608947No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG184700.016.0464295
GTGTAGG147800.014.2522511
TATACAG185000.013.6208745
CTATACT216700.013.5969584
GTATATA362500.013.3388561
GTGTAAG127350.013.0208251
GTATTAG190800.012.9107231
GTATAAG191450.012.866891
TCTATAC227750.012.66972353
GTATAGA227800.012.6096421
TTATACT306600.012.6052894
GTATAAT289800.012.25474551
CTAGTAC128850.011.8896823
CTTACAC124300.011.7996953
GTATAGG145450.011.789491
TAATACT274050.011.783824
CCTATAC154550.011.6303573
GTATTAT302550.011.5944571
GTACTAG143900.011.4930511
TAGTACT179200.011.4391794
Produced by FastQC (version 0.11.2)