##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename Kamla_M3_TCCTGAGC-TATCCTCT_L002_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 33803726 Sequences flagged as poor quality 0 Sequence length 93 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.054479852309775 33.0 33.0 33.0 33.0 33.0 2 32.02225091991338 33.0 33.0 33.0 33.0 33.0 3 32.05494595477433 33.0 33.0 33.0 33.0 33.0 4 35.810172198177206 37.0 37.0 37.0 33.0 37.0 5 35.80476101953968 37.0 37.0 37.0 33.0 37.0 6 35.872230357091404 37.0 37.0 37.0 33.0 37.0 7 35.84724704608007 37.0 37.0 37.0 33.0 37.0 8 35.826157388685495 37.0 37.0 37.0 33.0 37.0 9 35.869664071942836 37.0 37.0 37.0 33.0 37.0 10-11 35.85789749922834 37.0 37.0 37.0 33.0 37.0 12-13 35.83385250194017 37.0 37.0 37.0 33.0 37.0 14-15 37.770441400453905 40.0 37.0 40.0 33.0 40.0 16-17 37.721315588110016 40.0 37.0 40.0 33.0 40.0 18-19 37.690823801494545 40.0 37.0 40.0 33.0 40.0 20-21 37.65857024163549 40.0 37.0 40.0 33.0 40.0 22-23 37.59588692974259 40.0 37.0 40.0 33.0 40.0 24-25 37.50936418961626 40.0 37.0 40.0 33.0 40.0 26-27 37.440349696953525 40.0 37.0 40.0 33.0 40.0 28-29 37.368041159131394 40.0 37.0 40.0 33.0 40.0 30-31 37.25400725056167 40.0 37.0 40.0 33.0 40.0 32-33 37.17163869746193 40.0 37.0 40.0 33.0 40.0 34-35 37.099385848767085 40.0 37.0 40.0 33.0 40.0 36-37 36.97450449693031 40.0 37.0 40.0 33.0 40.0 38-39 36.855446867011054 40.0 37.0 40.0 33.0 40.0 40-41 36.831898368244964 40.0 37.0 40.0 33.0 40.0 42-43 36.858959186925134 40.0 37.0 40.0 33.0 40.0 44-45 36.772658700404804 40.0 37.0 40.0 33.0 40.0 46-47 36.640837876866 40.0 37.0 40.0 33.0 40.0 48-49 36.49540963324576 38.5 37.0 40.0 33.0 40.0 50-51 35.409068899682836 37.0 35.0 38.5 30.0 40.0 52-53 35.48678261088733 37.0 35.0 38.5 27.0 40.0 54-55 36.18238847398065 37.0 37.0 40.0 33.0 40.0 56-57 36.18527392512884 37.0 37.0 40.0 30.0 40.0 58-59 36.059110939427214 37.0 37.0 40.0 27.0 40.0 60-61 36.2331507775208 37.0 37.0 40.0 33.0 40.0 62-63 36.173022243169285 37.0 37.0 40.0 33.0 40.0 64-65 35.998651761051434 37.0 37.0 40.0 27.0 40.0 66-67 35.78926187012639 37.0 37.0 40.0 27.0 40.0 68-69 35.56343211987933 37.0 35.0 40.0 27.0 40.0 70-71 35.34495408878891 37.0 33.0 40.0 27.0 40.0 72-73 35.095940326223214 37.0 33.0 38.5 27.0 40.0 74-75 34.861802453374516 37.0 33.0 37.0 27.0 40.0 76-77 34.595679008876125 37.0 33.0 37.0 27.0 40.0 78-79 34.32334843797989 37.0 33.0 37.0 27.0 40.0 80-81 34.044061385422424 37.0 33.0 37.0 27.0 37.0 82-83 33.781375135983524 37.0 33.0 37.0 27.0 37.0 84-85 33.55970880251485 37.0 33.0 37.0 27.0 37.0 86-87 33.39719193972877 37.0 33.0 37.0 27.0 37.0 88-89 33.21227233944566 37.0 33.0 37.0 27.0 37.0 90-91 32.99224743745704 37.0 33.0 37.0 27.0 37.0 92-93 32.093092829470926 35.0 33.0 37.0 24.5 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2304 1 0.3435449429077444 2304 2 0.42002830942153224 2304 3 0.42683764081170494 2304 4 0.43667385670292447 2304 5 0.37700993306415853 2304 6 0.4860654185314033 2304 7 0.49742214442191823 2304 8 0.617564132808269 2304 9 0.5780673418459514 2304 10-11 0.6475888323357566 2304 12-13 0.6489974178571316 2304 14-15 0.8885639774126801 2304 16-17 0.9183597680786093 2304 18-19 0.9016047468575934 2304 20-21 0.8925690320903925 2304 22-23 0.9222768662827789 2304 24-25 0.9527536881287446 2304 26-27 0.9081279455691629 2304 28-29 0.8908954156258346 2304 30-31 0.9065360918696825 2304 32-33 0.8507294496565834 2304 34-35 0.8447307822058008 2304 36-37 0.8792873671508943 2304 38-39 0.8727001797678113 2304 40-41 0.8750393166408728 2304 42-43 0.7816322330356087 2304 44-45 0.8405258085024556 2304 46-47 0.7467884264591333 2304 48-49 0.7791836835002925 2304 50-51 0.7634673682456636 2304 52-53 0.7845054867110619 2304 54-55 0.8611038870218835 2304 56-57 0.8946682928141172 2304 58-59 0.8610045102001607 2304 60-61 0.8999926917726881 2304 62-63 0.8334521609612153 2304 64-65 0.909571092011717 2304 66-67 0.9194177519538798 2304 68-69 0.9368829857133036 2304 70-71 0.8796973962293038 2304 72-73 0.9143729048363554 2304 74-75 0.9080002347005163 2304 76-77 0.8872768141409466 2304 78-79 0.7991468750505675 2304 80-81 0.8489104203443247 2304 82-83 0.8305240340299349 2304 84-85 0.8036716773667507 2304 86-87 0.8060284075029784 2304 88-89 0.7528329622104621 2304 90-91 0.7480101844785594 2304 92-93 0.8266988906379957 2305 1 0.3236739023968376 2305 2 0.4481486625977027 2305 3 0.4818695354588556 2305 4 0.44438481536836605 2305 5 0.41004912437264807 2305 6 0.4246313686036558 2305 7 0.6010028604076538 2305 8 0.5992084997227778 2305 9 0.619740631912471 2305 10-11 0.6782815893220615 2305 12-13 0.6971273030381724 2305 14-15 0.9328143572382501 2305 16-17 0.9637766492332389 2305 18-19 0.9026271826699173 2305 20-21 0.9967558385504702 2305 22-23 0.9678397404369079 2305 24-25 0.9651762238925059 2305 26-27 0.9559373393926336 2305 28-29 0.9838753978175276 2305 30-31 0.9877405635397878 2305 32-33 0.8683034545007686 2305 34-35 0.943672815645705 2305 36-37 0.9372925983336629 2305 38-39 0.9910683577121802 2305 40-41 0.9923169453201126 2305 42-43 0.9410273355163241 2305 44-45 1.024257359048896 2305 46-47 0.9606847550508704 2305 48-49 1.0176672504039317 2305 50-51 0.9528051007199281 2305 52-53 1.0294102136759378 2305 54-55 1.1435181597839517 2305 56-57 1.0492619795572011 2305 58-59 1.0138647809777197 2305 60-61 1.1340434561190094 2305 62-63 1.0719949442674874 2305 64-65 1.0738381849161414 2305 66-67 1.023002978992352 2305 68-69 1.0801649856950846 2305 70-71 1.0855039772799984 2305 72-73 1.1151418017232757 2305 74-75 1.0499618002484468 2305 76-77 1.0683279556902932 2305 78-79 0.9899176760040973 2305 80-81 0.9575540417915107 2305 82-83 0.858578380663225 2305 84-85 1.0397527344272248 2305 86-87 1.0022719627237677 2305 88-89 0.8854655175468622 2305 90-91 0.9495437709571704 2305 92-93 0.9560082735834676 2306 1 0.39090968281773186 2306 2 0.4020962624853368 2306 3 0.5092124547990977 2306 4 0.5849783567814342 2306 5 0.4694879124623128 2306 6 0.5927541741728675 2306 7 0.6137357671873147 2306 8 0.6567233568884276 2306 9 0.6369681706649359 2306 10-11 0.6946853906288695 2306 12-13 0.6841951244895625 2306 14-15 0.9535153090609114 2306 16-17 0.9886624882738744 2306 18-19 0.9792554212300644 2306 20-21 0.9717413048183658 2306 22-23 0.9632718372614093 2306 24-25 0.9562282756385514 2306 26-27 0.8715820345561767 2306 28-29 0.912443288803658 2306 30-31 0.9969050599004419 2306 32-33 0.9819360571282445 2306 34-35 0.9561435577227613 2306 36-37 0.9374702270657522 2306 38-39 1.041147141829228 2306 40-41 0.9670139843587435 2306 42-43 1.0349316209345858 2306 44-45 0.9094043834880097 2306 46-47 0.9637420642887946 2306 48-49 0.9868272491683712 2306 50-51 0.9168793667404813 2306 52-53 1.0162940035192136 2306 54-55 1.0924880828192443 2306 56-57 1.1346761102370948 2306 58-59 1.1362114381570052 2306 60-61 1.1425521061309567 2306 62-63 1.1162118140070163 2306 64-65 1.1340461838501241 2306 66-67 1.1493402277876825 2306 68-69 1.168477697312035 2306 70-71 1.1465959990402155 2306 72-73 1.196732757468702 2306 74-75 1.1087587839917177 2306 76-77 1.084767528608424 2306 78-79 1.0553310157608493 2306 80-81 1.0913837255663097 2306 82-83 1.113305853620858 2306 84-85 1.0872074197949502 2306 86-87 1.0585172670504974 2306 88-89 1.059995572764663 2306 90-91 1.095146508410025 2306 92-93 1.0325520004133004 2307 1 0.27722456513722804 2307 2 0.3362927520822083 2307 3 0.40504340209276535 2307 4 0.4707027840806006 2307 5 0.4231965364819885 2307 6 0.3998952573630348 2307 7 0.4579335088136034 2307 8 0.5540699781536347 2307 9 0.535426481164123 2307 10-11 0.5354877208403437 2307 12-13 0.5818423867527756 2307 14-15 0.8190716077951308 2307 16-17 0.8244385617864722 2307 18-19 0.7074878250854937 2307 20-21 0.7228564191738087 2307 22-23 0.7789990713642325 2307 24-25 0.8492970869700329 2307 26-27 0.8578930812527616 2307 28-29 0.8328789232028626 2307 30-31 0.8285155314844985 2307 32-33 0.8198345642370981 2307 34-35 0.8154101789263919 2307 36-37 0.8249272142109021 2307 38-39 0.7630941784192657 2307 40-41 0.7565095473512145 2307 42-43 0.7833410304487813 2307 44-45 0.7075374613868135 2307 46-47 0.790555454435534 2307 48-49 0.7972033018480289 2307 50-51 0.7094647997887265 2307 52-53 0.7874416141955365 2307 54-55 0.8574539778458075 2307 56-57 0.9554968334790743 2307 58-59 0.900775054592664 2307 60-61 0.854341682062433 2307 62-63 0.8552816228570137 2307 64-65 0.8613351014399058 2307 66-67 0.8969398462265872 2307 68-69 0.9507784912218327 2307 70-71 0.9148195437612685 2307 72-73 0.9525388258089862 2307 74-75 0.918512170115541 2307 76-77 0.876495885945829 2307 78-79 0.8493963321856342 2307 80-81 0.8184666483981857 2307 82-83 0.8415949579887965 2307 84-85 0.8958213693525323 2307 86-87 0.8741181821608492 2307 88-89 0.9528523216137188 2307 90-91 0.876693121362571 2307 92-93 0.7601742964915736 2308 1 0.3222728798788772 2308 2 0.38137307749966 2308 3 0.40562522813617363 2308 4 0.480929572050492 2308 5 0.45874771675602943 2308 6 0.4945132674802153 2308 7 0.4999578807552254 2308 8 0.5725894043206807 2308 9 0.5768806544907363 2308 10-11 0.602192764063183 2308 12-13 0.5715487907041279 2308 14-15 0.7689976529193885 2308 16-17 0.7972532011498501 2308 18-19 0.7244296845827449 2308 20-21 0.7658747823044294 2308 22-23 0.8025368579341574 2308 24-25 0.8149655796234825 2308 26-27 0.7925130685219202 2308 28-29 0.7754102000932619 2308 30-31 0.7892864468486067 2308 32-33 0.6987532838340016 2308 34-35 0.7711282155889876 2308 36-37 0.836506809307302 2308 38-39 0.8944476372112007 2308 40-41 0.8593486946059201 2308 42-43 0.8626804831184174 2308 44-45 0.8224594288257379 2308 46-47 0.8228149776360425 2308 48-49 0.9067239720031637 2308 50-51 0.7672665821109277 2308 52-53 0.778853695137947 2308 54-55 0.9120608918686486 2308 56-57 0.9741795716399935 2308 58-59 0.9509390997389175 2308 60-61 0.976090335421496 2308 62-63 0.9729881982647441 2308 64-65 0.9681956412753081 2308 66-67 1.0325321025998235 2308 68-69 1.0553563130792938 2308 70-71 1.0017800549657494 2308 72-73 0.9615526925862881 2308 74-75 0.9644481890930692 2308 76-77 0.954935906243449 2308 78-79 0.9103668131805449 2308 80-81 0.946495971869119 2308 82-83 0.9741622242014714 2308 84-85 0.9763323077327897 2308 86-87 0.9409460140200281 2308 88-89 1.0024409960829672 2308 90-91 0.9635540613817781 2308 92-93 0.9839997768882291 2309 1 0.4172988625440759 2309 2 0.4472301248072057 2309 3 0.457922559786752 2309 4 0.5379515722797947 2309 5 0.5294826683034515 2309 6 0.5384859648589284 2309 7 0.580294783620225 2309 8 0.6226447219868163 2309 9 0.5835329059337084 2309 10-11 0.5631256837566809 2309 12-13 0.6099980582432707 2309 14-15 0.7842391528817316 2309 16-17 0.8157606940629307 2309 18-19 0.8061440196474123 2309 20-21 0.7651686323368665 2309 22-23 0.7097730447352362 2309 24-25 0.8391275397529299 2309 26-27 0.7180585798342278 2309 28-29 0.7534985372323462 2309 30-31 0.7504829122663494 2309 32-33 0.6751935683808128 2309 34-35 0.7606464340610017 2309 36-37 0.7451167540653358 2309 38-39 0.7153309046278977 2309 40-41 0.7305146841098704 2309 42-43 0.7294097958991657 2309 44-45 0.7798369857127625 2309 46-47 0.7015987051336339 2309 48-49 0.6652051977869746 2309 50-51 0.6376711819235155 2309 52-53 0.7412284373385631 2309 54-55 0.6687958577105917 2309 56-57 0.7602699447960362 2309 58-59 0.7039426995441502 2309 60-61 0.7108501153946563 2309 62-63 0.735075966445109 2309 64-65 0.7760213773207454 2309 66-67 0.8285092375569505 2309 68-69 0.8623261161379574 2309 70-71 0.9222423234503765 2309 72-73 0.9204581333152291 2309 74-75 0.8553130775019966 2309 76-77 0.8469256248947232 2309 78-79 0.8323936633592837 2309 80-81 0.7790870105355765 2309 82-83 0.8581369077853651 2309 84-85 0.8631683539910995 2309 86-87 0.8786920475521214 2309 88-89 0.8171663893726304 2309 90-91 0.7998666545196969 2309 92-93 0.7587448705375799 2310 1 0.41134186884843515 2310 2 0.4753638012556891 2310 3 0.48557474761270925 2310 4 0.5330480115571561 2310 5 0.5530157614591715 2310 6 0.5885464338995234 2310 7 0.6211363540345758 2310 8 0.5995891712581738 2310 9 0.5347818887795626 2310 10-11 0.5740249799405746 2310 12-13 0.5881770883413964 2310 14-15 0.7702069139624328 2310 16-17 0.7663543016960119 2310 18-19 0.7241148901673995 2310 20-21 0.6997266202045935 2310 22-23 0.7621969165115772 2310 24-25 0.7679043963618 2310 26-27 0.6980944807129603 2310 28-29 0.7739251147375512 2310 30-31 0.7337252008561705 2310 32-33 0.7099104983140236 2310 34-35 0.7577529387470392 2310 36-37 0.8456373249763942 2310 38-39 0.8183453721083183 2310 40-41 0.7792634912186358 2310 42-43 0.7977733241918799 2310 44-45 0.7927386940611285 2310 46-47 0.8099633367777699 2310 48-49 0.8153174739196629 2310 50-51 0.7701608294442437 2310 52-53 0.8055006642058373 2310 54-55 0.8434917363348546 2310 56-57 0.8485370790898372 2310 58-59 0.8058292527735134 2310 60-61 0.9107834235919583 2310 62-63 0.8645995763862686 2310 64-65 0.8551613243738672 2310 66-67 0.8829640915570138 2310 68-69 0.9361947580644525 2310 70-71 0.9021172324190445 2310 72-73 0.9153322728142683 2310 74-75 0.8864287456387174 2310 76-77 0.8425418717928466 2310 78-79 0.8410228036797349 2310 80-81 0.8457868232649162 2310 82-83 0.9415900811042022 2310 84-85 0.9401126182123818 2310 86-87 0.8475157268640885 2310 88-89 0.8123946803970483 2310 90-91 0.8615607480034342 2310 92-93 0.7880998433510165 2311 1 0.2459678100182323 2311 2 0.312904335499141 2311 3 0.35806725819137597 2311 4 0.41456054096106243 2311 5 0.4053045921618761 2311 6 0.4430300859454306 2311 7 0.43848110885455327 2311 8 0.48490975491672117 2311 9 0.46519405988205165 2311 10-11 0.45649103500635846 2311 12-13 0.4801641272092354 2311 14-15 0.5896295369177054 2311 16-17 0.5209939724302899 2311 18-19 0.5591538939310681 2311 20-21 0.5566268397272296 2311 22-23 0.5845982519937607 2311 24-25 0.583778300683143 2311 26-27 0.5284338850606289 2311 28-29 0.4995319916621952 2311 30-31 0.41864607946050114 2311 32-33 0.42014555343320126 2311 34-35 0.4876831406268707 2311 36-37 0.598531211226387 2311 38-39 0.43708568128518266 2311 40-41 0.4363289955675427 2311 42-43 0.50208226129498 2311 44-45 0.4212058154720211 2311 46-47 0.5441652576123843 2311 48-49 0.5369417066006079 2311 50-51 0.4735028815845723 2311 52-53 0.4457287108459411 2311 54-55 0.5589006687616376 2311 56-57 0.5850565851040415 2311 58-59 0.5407484587770526 2311 60-61 0.5166308556781232 2311 62-63 0.4405377065940499 2311 64-65 0.546650660781907 2311 66-67 0.5780982571840596 2311 68-69 0.5783998848309437 2311 70-71 0.6236733360952584 2311 72-73 0.6108904826919783 2311 74-75 0.5988814776105542 2311 76-77 0.6896936355679912 2311 78-79 0.47655203928056267 2311 80-81 0.4745641697593683 2311 82-83 0.5269724616331359 2311 84-85 0.5604252662126186 2311 86-87 0.4487921381704041 2311 88-89 0.5611844011525164 2311 90-91 0.5551614068798543 2311 92-93 0.39851413788897005 2313 1 0.2262822925919572 2313 2 0.22887745257759207 2313 3 0.23848609057190373 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0.20456064952839625 1114 64-65 0.2124576457918721 1114 66-67 0.2880530201536686 1114 68-69 0.2909094165703081 1114 70-71 0.26465405489940963 1114 72-73 0.22498390519513123 1114 74-75 0.09324967696027642 1114 76-77 0.1575528399828272 1114 78-79 0.3017767386745476 1114 80-81 0.33605914644209633 1114 82-83 0.4153434300229293 1114 84-85 0.39019696580702146 1114 86-87 0.2831175926734204 1114 88-89 0.33613402959130667 1114 90-91 0.32079778175953066 1114 92-93 0.277066538892381 1115 1 0.13803705099826757 1115 2 0.043646129320521254 1115 3 0.04604873199177817 1115 4 0.20568769150983712 1115 5 0.21778580227589828 1115 6 0.21112919507857697 1115 7 0.22158765711696304 1115 8 0.22654076733436312 1115 9 -0.5109229545429699 1115 10-11 -0.004341308076440953 1115 12-13 -0.2347200868256749 1115 14-15 -0.027195375839021096 1115 16-17 -0.2705522517982999 1115 18-19 0.10356473380537068 1115 20-21 0.18009726726582187 1115 22-23 -0.14959352681271554 1115 24-25 0.0024438296682234295 1115 26-27 -0.05671674716295172 1115 28-29 0.007206241349365428 1115 30-31 0.16636122584797874 1115 32-33 0.00465263037146002 1115 34-35 -0.11877350068280634 1115 36-37 -1.1661309660426156 1115 38-39 -0.22749225867105594 1115 40-41 0.07159151557704035 1115 42-43 0.16549660696068713 1115 44-45 0.3026511086528245 1115 46-47 0.2881400882736713 1115 48-49 0.021921407572513374 1115 50-51 0.09168546963362445 1115 52-53 0.13823196891883782 1115 54-55 0.14262114324161246 1115 56-57 0.19731206694338255 1115 58-59 0.15112551622754467 1115 60-61 0.2011491735069768 1115 62-63 0.05813761688796859 1115 64-65 0.11976257084248232 1115 66-67 -0.07341788518002801 1115 68-69 0.11041857318254955 1115 70-71 -0.9954226013661867 1115 72-73 -0.39390690396480466 1115 74-75 -0.6138480101871053 1115 76-77 -0.04178366698400282 1115 78-79 0.05277494349016365 1115 80-81 0.06393877878404908 1115 82-83 0.1858009012965809 1115 84-85 -0.36426445054274836 1115 86-87 0.026086825243531564 1115 88-89 0.1495076161127713 1115 90-91 0.2507691243293735 1115 92-93 0.2022114777288131 1116 1 0.04280462353025172 1116 2 0.09802260427429488 1116 3 0.09711428463115368 1116 4 0.1726458816428078 1116 5 0.1264155065940713 1116 6 -0.06301596066548854 1116 7 -0.3782529287814569 1116 8 -0.1348314825549224 1116 9 -0.011051166332073592 1116 10-11 -0.29701807173821493 1116 12-13 -0.36334029795264655 1116 14-15 -0.40653507368901387 1116 16-17 -1.0009320446840775 1116 18-19 -0.8491037627864557 1116 20-21 -0.42148211846348715 1116 22-23 -0.18857650994228692 1116 24-25 -0.030385114291611615 1116 26-27 -0.004585837973515083 1116 28-29 -0.02348534767122601 1116 30-31 -0.06879594201315342 1116 32-33 -0.45011388850543455 1116 34-35 -0.539876888189113 1116 36-37 -0.5494278867147528 1116 38-39 -0.2710982217229372 1116 40-41 -0.14361572274778922 1116 42-43 -0.03241840780465566 1116 44-45 -0.3145448374438047 1116 46-47 -0.03900455153461735 1116 48-49 0.036570393960722924 1116 50-51 0.11599857051889728 1116 52-53 0.12390585879375493 1116 54-55 -0.028176050031476052 1116 56-57 -0.4504005631621055 1116 58-59 -0.42708120595936094 1116 60-61 -0.14196732408795043 1116 62-63 -0.043017082090493375 1116 64-65 -0.42613044284512114 1116 66-67 -0.5390205347588406 1116 68-69 -0.15208852929672645 1116 70-71 -0.06451288372220176 1116 72-73 -0.028058598175121574 1116 74-75 -0.11341275692328878 1116 76-77 -0.15381489170957252 1116 78-79 0.013578680759849249 1116 80-81 -0.350957286834209 1116 82-83 -0.01813752547109715 1116 84-85 -0.04284142896113963 1116 86-87 -0.005045037316776302 1116 88-89 0.04367824212391014 1116 90-91 0.12957973520472876 1116 92-93 0.07960520300035867 2301 1 -0.44813676726481333 2301 2 -0.7283517809720195 2301 3 -0.6004759433513236 2301 4 -0.4452048536611173 2301 5 -0.34887770950734875 2301 6 -0.41968813691799056 2301 7 -0.4157826782951517 2301 8 -0.30304119586448053 2301 9 -1.1955640042709206 2301 10-11 -0.8476953138873426 2301 12-13 -0.7484007310487115 2301 14-15 -0.7914383832293126 2301 16-17 -1.0582647267160539 2301 18-19 -0.9532017329465461 2301 20-21 -0.8857407042366532 2301 22-23 -0.9953114586392857 2301 24-25 -1.1995556001138894 2301 26-27 -0.8868035147730282 2301 28-29 -0.7436930319873056 2301 30-31 -1.2720565574970166 2301 32-33 -0.9842155639071706 2301 34-35 -0.9988898862552773 2301 36-37 -1.02663348166908 2301 38-39 -1.000291487263084 2301 40-41 -1.1595299911001646 2301 42-43 -1.1942026120672864 2301 44-45 -0.973357983180648 2301 46-47 -0.7261843266083403 2301 48-49 -0.7544430520576455 2301 50-51 -0.6478491629074696 2301 52-53 -0.8711406465852107 2301 54-55 -1.0127743759364591 2301 56-57 -0.9153415759929047 2301 58-59 -0.9240063578669577 2301 60-61 -1.0043097555144413 2301 62-63 -0.9358697255834372 2301 64-65 -0.9530867165108674 2301 66-67 -0.8792875928866337 2301 68-69 -0.8955021931547407 2301 70-71 -0.9908157569154739 2301 72-73 -0.9664338552678373 2301 74-75 -0.9367515399656625 2301 76-77 -1.1475651571725223 2301 78-79 -1.023912818925595 2301 80-81 -0.9231976924485679 2301 82-83 -0.8331325361141211 2301 84-85 -0.7704789172957618 2301 86-87 -0.7845679248775212 2301 88-89 -0.8495163174656568 2301 90-91 -0.7612517318928695 2301 92-93 -1.3281292167414627 2302 1 -0.14556613533756746 2302 2 -0.06203873368225388 2302 3 -0.1505226711819745 2302 4 -0.08200662103750744 2302 5 -0.21642870260119196 2302 6 -0.14272587616518706 2302 7 -0.10258794084585077 2302 8 -0.6555279959449649 2302 9 -0.5099227242798108 2302 10-11 -0.18975277473537489 2302 12-13 -0.2672082173942343 2302 14-15 -0.6678355381472656 2302 16-17 -0.5411735495515515 2302 18-19 -0.339169840466262 2302 20-21 -0.6538416203328126 2302 22-23 -0.29358746804906133 2302 24-25 -0.24106559869296262 2302 26-27 -0.2246345581468958 2302 28-29 -0.1420304398997274 2302 30-31 -0.9068843805233513 2302 32-33 -0.4005401636920354 2302 34-35 -0.20772384678389244 2302 36-37 -0.1270012659275892 2302 38-39 -1.2331205775340806 2302 40-41 -0.4456684942416729 2302 42-43 -0.20894916543073805 2302 44-45 -0.06968307018689757 2302 46-47 -0.34217780938973874 2302 48-49 -0.3543978039418505 2302 50-51 -0.11442033129335272 2302 52-53 -0.40842132550495336 2302 54-55 -0.17968342747387567 2302 56-57 -0.2680801130570529 2302 58-59 -0.20365408912817173 2302 60-61 -0.27693224136005057 2302 62-63 -0.8856370376971654 2302 64-65 -0.49469230130784325 2302 66-67 -0.4904715007412008 2302 68-69 -0.8218097667981894 2302 70-71 -1.2271916456003424 2302 72-73 -0.8130842439186878 2302 74-75 -0.3572440672484234 2302 76-77 -0.2911457061670575 2302 78-79 -0.09015814193062965 2302 80-81 -0.19758570387968888 2302 82-83 -0.1304923679691754 2302 84-85 -0.15067140704216797 2302 86-87 -0.2156923373801476 2302 88-89 -0.2853138541987761 2302 90-91 -0.1915692494008141 2302 92-93 -0.29539503153046454 2303 1 -0.28134364818996005 2303 2 -0.2327966813190443 2303 3 -0.3256674361927736 2303 4 -0.6355953360982411 2303 5 -0.46814381279712336 2303 6 -0.6334109324793289 2303 7 -0.9818294540460784 2303 8 -0.8086996469342722 2303 9 -0.5120455244857425 2303 10-11 -0.5749560659034074 2303 12-13 -0.657871440260692 2303 14-15 -1.170145584240224 2303 16-17 -1.0421579809188444 2303 18-19 -0.8875028421858886 2303 20-21 -1.3244242710212575 2303 22-23 -1.780884358101268 2303 24-25 -1.2536380358512744 2303 26-27 -1.2223432349155487 2303 28-29 -0.8387041421477122 2303 30-31 -0.6231055861680943 2303 32-33 -0.6143981816374193 2303 34-35 -0.44723159481479513 2303 36-37 -0.48239931577797535 2303 38-39 -0.49317435043829505 2303 40-41 -0.4875527105147981 2303 42-43 -0.3404722788878942 2303 44-45 -0.37389938720919247 2303 46-47 -0.40102263668605076 2303 48-49 -0.2684670922907557 2303 50-51 -0.22768590652570708 2303 52-53 -0.21427448170218355 2303 54-55 -0.33163268438970306 2303 56-57 -0.2745220288626058 2303 58-59 -0.2853622630009838 2303 60-61 -0.41410262978534007 2303 62-63 -0.3214799728616313 2303 64-65 -0.3498813491886352 2303 66-67 -0.5852120970124943 2303 68-69 -0.39183276464731165 2303 70-71 -0.38413406048569954 2303 72-73 -0.797657149249666 2303 74-75 -0.8733540883765443 2303 76-77 -0.5554754589273045 2303 78-79 -0.35350129676692177 2303 80-81 -0.30375884239242623 2303 82-83 -0.541192334021865 2303 84-85 -0.37641843795303487 2303 86-87 -0.3477321265336286 2303 88-89 -0.2570159072826783 2303 90-91 -0.2234942751823965 2303 92-93 -0.1841670851498982 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 78991.0 3 3295.0 4 1863.0 5 1463.0 6 1452.0 7 2648.0 8 5877.0 9 8220.0 10 11835.0 11 19143.0 12 30820.0 13 44017.0 14 54141.0 15 63539.0 16 71625.0 17 80932.0 18 90862.0 19 101875.0 20 113860.0 21 126365.0 22 142854.0 23 161230.0 24 183678.0 25 210382.0 26 244487.0 27 286608.0 28 340492.0 29 409933.0 30 506648.0 31 647169.0 32 845262.0 33 1141737.0 34 1603212.0 35 2402136.0 36 4038078.0 37 8111458.0 38 1.1108171E7 39 507368.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.80259887329035 23.657850682411166 10.445696212107087 24.093854232191394 2 15.911372356281383 22.108490079099465 38.67160666818856 23.308530896430593 3 15.618214289997933 26.75449180231308 31.632133631185617 25.995160276503377 4 11.725497056313117 16.77267039127111 42.23217722380987 29.269655328605904 5 12.088243059033642 37.989657013119206 37.783110247643215 12.13898968020394 6 28.789135126528965 41.341367070988326 18.325157313730273 11.544340488752438 7 26.247813665205854 32.63584232032121 25.72959661666897 15.38674739780396 8 23.437349478338927 36.438673853859186 23.32853997945807 16.795436688343813 9 22.8448139164483 14.922747132916086 26.435888603550033 35.796550347085585 10-11 22.376364220890217 27.019097646249225 31.63399108521184 18.970547047648726 12-13 21.54459869861154 25.93507984344705 31.84362603741574 20.67669542052567 14-15 19.49083384834455 27.31780259613111 27.568365588716254 25.622997966808086 16-17 19.82228424421754 31.720153593417894 28.56027562877908 19.897286533585493 18-19 19.937488111617828 30.469735736139818 30.088755860443193 19.504020291799158 20-21 20.16197014969638 30.050761827793554 29.895915416725366 19.8913526057847 22-23 20.297775931474437 29.796145490469673 29.8190074345215 20.08707114353439 24-25 19.88178090341327 29.67211152246713 30.14874790085276 20.297359673266836 26-27 20.151710683396885 30.23114459693343 29.80524709096813 19.811897628701562 28-29 20.13229956857392 30.07692816942925 29.809169579392346 19.981602682604482 30-31 19.957467703206884 29.891540032997128 30.028280859981482 20.122711403814503 32-33 20.135495398822844 30.17753545125823 29.850737547030263 19.83623160288866 34-35 19.988164622167147 29.861798023682358 29.992504547473086 20.157532806677413 36-37 19.963373891115406 30.072303336402147 30.025187031072704 19.939135741409746 38-39 20.033455888345657 30.05580732524186 30.024531420967097 19.886205365445377 40-41 19.89925678795901 29.95346203826003 30.173202470523297 19.97407870325766 42-43 19.920071397549304 30.001077394382204 30.1522626424088 19.9265885656597 44-45 19.87317909351833 29.862974577200358 30.14855656480365 20.115289764477662 46-47 19.822983490466 29.843654740909507 30.303071064835013 20.030290703789476 48-49 19.83134908190283 30.08175197149223 30.183626848218832 19.903272098386108 50-51 19.73889941617484 30.00485050355933 30.283128644198836 19.973121436067004 52-53 19.81196356883111 29.869553581026732 30.39682988037914 19.92165296976302 54-55 19.791647789939194 29.98233123478993 30.2742070877276 19.951813887543278 56-57 19.755647697628266 29.9774291993947 30.21518453561754 20.051738567359497 58-59 19.828168149193136 29.98358072821972 30.166599892964346 20.0216512296228 60-61 19.666916061367264 29.932515114038193 30.33434388985579 20.066224934738756 62-63 19.683548150479588 29.99664433023955 30.2424968983117 20.077310620969165 64-65 19.80318103705574 29.902114361355803 30.263241106344186 20.031463495244274 66-67 19.760939626035867 29.826008439497958 30.21747380281075 20.19557813165542 68-69 19.756454933663548 29.972316516423643 30.140439833592385 20.13078871632042 70-71 19.874996716696426 29.95249098661793 30.098228878895583 20.074283417790063 72-73 19.872715155648184 29.94947030809689 30.019188269101516 20.158626267153416 74-75 19.87293336876457 30.065251045486207 30.043644100667127 20.018171485082096 76-77 19.869123277939917 30.033696550887317 30.029639066115898 20.06754110505687 78-79 20.021556930237494 29.99038923530289 29.966600712997227 20.02145312146239 80-81 19.965314366910825 30.08411896267565 29.9247860064009 20.025780664012625 82-83 20.057306331022293 30.08182665016554 29.945855782186527 19.91501123662565 84-85 20.028930056296275 30.119230844757404 29.941328306913167 19.91051079203315 86-87 19.909993379589356 30.00730524491552 30.043593453251304 20.039107922243815 88-89 20.040393130299723 29.983621869498663 29.946704007085906 20.029280993115712 90-91 20.203164937268923 30.064777527260777 29.871503760424282 19.860553775046018 92-93 20.285732969503606 29.944550665246698 29.82593381134177 19.943782553907923 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 74984.0 1 38768.5 2 2230.0 3 1780.5 4 1572.0 5 1427.0 6 1425.0 7 1526.0 8 1716.0 9 2029.0 10 2572.0 11 3548.0 12 5037.0 13 7264.5 14 8599.0 15 10728.5 16 16209.5 17 25318.0 18 39544.5 19 61124.5 20 92123.5 21 134020.5 22 190469.0 23 263555.0 24 351151.0 25 454790.0 26 576112.5 27 711309.0 28 853961.5 29 926051.0 30 993010.0 31 1121808.5 32 1238657.0 33 1339675.5 34 1424608.0 35 1489907.5 36 1526883.5 37 1535926.5 38 1521816.5 39 1488463.0 40 1444312.5 41 1390332.5 42 1328594.0 43 1297485.0 44 1258604.0 45 1169421.5 46 1073917.5 47 981244.5 48 887704.0 49 799182.5 50 715823.5 51 638632.0 52 566904.5 53 500472.5 54 441479.0 55 389071.5 56 343073.5 57 321478.0 58 304931.0 59 272501.5 60 239546.5 61 210161.0 62 185009.5 63 161099.0 64 140591.5 65 123529.0 66 108182.5 67 94251.0 68 80458.5 69 66522.0 70 53789.0 71 47412.0 72 43251.5 73 36002.5 74 30238.5 75 26195.5 76 23911.5 77 21241.0 78 17535.0 79 14620.5 80 11980.5 81 9683.0 82 8094.5 83 6378.5 84 4899.0 85 3398.0 86 2629.0 87 2238.0 88 1606.0 89 1094.0 90 714.0 91 516.0 92 360.5 93 259.0 94 200.0 95 147.0 96 100.5 97 68.0 98 55.0 99 32.5 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35651099526720814 2 0.36077383895491283 3 0.3573866383841829 4 0.36337414402187496 5 0.37453563550952934 6 0.2978724889676363 7 0.28923438794883144 8 0.2899946591686372 9 0.2841254836818876 10-11 0.2810843988026645 12-13 0.27273029014612177 14-15 0.2640241492905249 16-17 0.2605881375325312 18-19 0.25576914213539653 20-21 0.25285969954909704 22-23 0.2612374742358283 24-25 0.2531362962769252 26-27 0.2637549481971307 28-29 0.26336741695279386 30-31 0.2650048104164612 32-33 0.26918482299850616 34-35 0.27991145118144667 36-37 0.2672604789188032 38-39 0.27355120556828555 40-41 0.27120678945273663 42-43 0.2744224113046 44-45 0.27849592675079665 46-47 0.26550919268485373 48-49 0.24538419226330255 50-51 0.2592584024613145 52-53 0.24435471995010255 54-55 0.2578561901726455 56-57 0.2565161012132213 58-59 0.2599062600377248 60-61 0.27246552643338784 62-63 0.26812133076691014 64-65 0.27015956761689525 66-67 0.2749785630140299 68-69 0.27087842328387113 70-71 0.2706550751239671 72-73 0.2536081377538086 74-75 0.25948175062121853 76-77 0.2610880824202634 78-79 0.2599905702702714 80-81 0.25617294377548794 82-83 0.2724344647687654 84-85 0.2713547021414148 86-87 0.2589640562108449 88-89 0.26133065922969556 90-91 0.25673501199246496 92-93 0.25043393145477516 >>END_MODULE >>Sequence Length Distribution pass #Length Count 93 3.3803726E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.87932881141204 #Duplication Level Percentage of deduplicated Percentage of total 1 88.62433879418211 65.4750666646936 2 7.31599934909257 10.810022429913728 3 1.4611255067349398 3.23840915240435 4 0.7376748629853724 2.1799569503363863 5 0.3945974560259234 1.4576297600942947 6 0.2951399276979351 1.3082843858263273 7 0.20722484397232502 1.0716742670007469 8 0.12230125309634898 0.7228427593242314 9 0.11997253981501739 0.7977141645600504 >10 0.6708887983032775 8.963784866318134 >50 0.03774684755407836 1.933851362893932 >100 0.012684213787159596 1.5295150596468372 >500 2.335442635703822E-4 0.10754872336048539 >1k 4.922622316118781E-5 0.06315583181032353 >5k 1.453461640371374E-5 0.07903905440872015 >10k+ 8.301649716708254E-6 0.2615045674078721 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 76539 0.2264217855747618 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 5.91650754712661E-6 3 0.0 0.0 8.874761320689915E-6 4 0.0 8.874761320689915E-6 1.4791268867816525E-5 5 2.366603018850644E-5 8.874761320689915E-6 1.4791268867816525E-5 6 2.958253773563305E-5 1.4791268867816525E-5 1.774952264137983E-5 7 3.254079150919635E-5 1.4791268867816525E-5 2.0707776414943134E-5 8 4.141555282988627E-5 1.4791268867816525E-5 2.0707776414943134E-5 9 4.437380660344957E-5 1.4791268867816525E-5 3.254079150919635E-5 10-11 5.029031415057618E-5 1.922864952816148E-5 6.951896367873766E-5 12-13 5.91650754712661E-5 2.6624283962069744E-5 7.691459811264592E-5 14-15 6.8039836791956E-5 2.958253773563305E-5 8.874761320689915E-5 16-17 9.02267400936808E-5 3.549904528275966E-5 1.2572578537644046E-4 18-19 1.1093451650862394E-4 3.993642594310462E-5 1.9080736839483317E-4 20-21 1.316422929235671E-4 5.029031415057618E-5 2.8842974292242224E-4 22-23 1.5678744999885516E-4 5.324856792413949E-5 4.55571081128749E-4 24-25 1.6862046509310836E-4 6.064420235804775E-5 0.001013201917445432 26-27 1.8341173396092488E-4 6.656070990517436E-5 0.005052697445246125 28-29 2.0707776414943135E-4 7.099809056551932E-5 0.026041507968677775 30-31 2.2482728679081116E-4 8.283110565977254E-5 0.04496249910438867 32-33 2.3518117499828273E-4 8.578935943333584E-5 0.06268835571558 34-35 2.4701419009253594E-4 8.72684863201175E-5 0.09348673575214755 36-37 2.558889514132259E-4 9.466412075402576E-5 0.16173217118136624 38-39 2.603263320735708E-4 9.91015014143707E-5 0.2752921379140276 40-41 2.647637127339158E-4 1.0353888207471567E-4 0.4047615934409124 42-43 2.7807585471495064E-4 1.0353888207471567E-4 0.5351140877192059 44-45 2.795549816017323E-4 1.1241364339540558E-4 0.6909460217492001 46-47 2.9138799669598556E-4 1.1389277028218724E-4 0.8759729622704906 48-49 3.1505402688449197E-4 1.183301509425322E-4 1.0601301761823534 50-51 3.3576180329943507E-4 1.1980927782931385E-4 1.241830560335272 52-53 3.5499045282759656E-4 1.212884047160955E-4 1.4156072617557012 54-55 3.8161473678966634E-4 1.2276753160287714E-4 1.59727066773645 56-57 3.919686249971379E-4 1.2868403915000377E-4 1.8001876479533647 58-59 4.141555282988627E-4 1.3312141981034872E-4 2.0059504683004468 60-61 4.718414768833471E-4 1.3460054669713037E-4 2.2069475418183186 62-63 4.7775798443047376E-4 1.3903792735747532E-4 2.3983450818409784 64-65 4.895909995247269E-4 1.4495443490460192E-4 2.586674912700452 66-67 5.029031415057618E-4 1.5087094245172855E-4 2.7896554953734984 68-69 5.206526641471417E-4 1.5382919622529184E-4 2.9985880846389534 70-71 5.561517094299013E-4 1.5678744999885516E-4 3.203185352999252 72-73 5.605890900902463E-4 1.5974570377241847E-4 3.400450589381774 74-75 5.679847245241545E-4 1.6566221131954505E-4 3.591740153141698 76-77 5.798177396184078E-4 1.6862046509310836E-4 3.7892731114907274 78-79 5.901716278258793E-4 1.7305784575345333E-4 3.9964810388061958 80-81 6.020046429201326E-4 1.804534801873616E-4 4.203316226146194 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACTG 14665 0.0 14.221033 5 GTATTAG 15205 0.0 13.487462 1 GTATATA 28710 0.0 13.300322 1 CTTACAC 10820 0.0 13.158565 3 GTATAGG 11670 0.0 13.133109 1 GTATAAG 16115 0.0 13.104099 1 GTGTAGG 12685 0.0 13.009018 1 TACACCG 6475 0.0 12.574176 5 GTGTAAG 11080 0.0 12.535645 1 GTATAAT 22995 0.0 12.383406 1 GTATAGA 18335 0.0 12.20613 1 TATACCG 5570 0.0 12.037693 5 GTATTAT 23830 0.0 11.711966 1 TATACAG 14895 0.0 11.429151 5 GTCCTAG 12195 0.0 11.353796 1 CTATACT 16685 0.0 11.299201 4 TCTATAC 18150 0.0 11.202844 3 CTAATAC 15195 0.0 11.08915 3 TTATACT 24820 0.0 11.069136 4 GTCTTAG 12685 0.0 11.052517 1 >>END_MODULE