FastQCFastQC Report
Wed 5 Nov 2014
Kamla_M3_TCCTGAGC-TATCCTCT_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameKamla_M3_TCCTGAGC-TATCCTCT_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33803726
Sequences flagged as poor quality0
Sequence length93
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN765390.2264217855747618No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG146650.014.2210335
GTATTAG152050.013.4874621
GTATATA287100.013.3003221
CTTACAC108200.013.1585653
GTATAGG116700.013.1331091
GTATAAG161150.013.1040991
GTGTAGG126850.013.0090181
TACACCG64750.012.5741765
GTGTAAG110800.012.5356451
GTATAAT229950.012.3834061
GTATAGA183350.012.206131
TATACCG55700.012.0376935
GTATTAT238300.011.7119661
TATACAG148950.011.4291515
GTCCTAG121950.011.3537961
CTATACT166850.011.2992014
TCTATAC181500.011.2028443
CTAATAC151950.011.089153
TTATACT248200.011.0691364
GTCTTAG126850.011.0525171
Produced by FastQC (version 0.11.2)