##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename Kamla_M3_TCCTGAGC-TATCCTCT_L002_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 33803726 Sequences flagged as poor quality 0 Sequence length 93 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.50150767403569 33.0 33.0 33.0 33.0 33.0 2 32.55434164269347 33.0 33.0 33.0 33.0 33.0 3 32.63942912683649 33.0 33.0 33.0 33.0 33.0 4 36.659252474120755 37.0 37.0 37.0 37.0 37.0 5 36.52459595726223 37.0 37.0 37.0 37.0 37.0 6 36.47285772580218 37.0 37.0 37.0 37.0 37.0 7 36.43361018841532 37.0 37.0 37.0 37.0 37.0 8 36.441353772658076 37.0 37.0 37.0 37.0 37.0 9 36.439563910794924 37.0 37.0 37.0 37.0 37.0 10-11 36.40565973407784 37.0 37.0 37.0 37.0 37.0 12-13 36.42169530364789 37.0 37.0 37.0 37.0 37.0 14-15 38.55641320427222 40.0 37.0 40.0 37.0 40.0 16-17 38.541627156722306 40.0 37.0 40.0 37.0 40.0 18-19 38.468968198949426 40.0 37.0 40.0 37.0 40.0 20-21 38.50101225527624 40.0 37.0 40.0 37.0 40.0 22-23 38.426995532977635 40.0 37.0 40.0 37.0 40.0 24-25 38.4151176115911 40.0 37.0 40.0 37.0 40.0 26-27 38.356280754376016 40.0 37.0 40.0 37.0 40.0 28-29 38.21803136435315 40.0 37.0 40.0 37.0 40.0 30-31 38.2233779643108 40.0 37.0 40.0 37.0 40.0 32-33 38.159390728110864 40.0 37.0 40.0 37.0 40.0 34-35 38.103609199766915 40.0 37.0 40.0 37.0 40.0 36-37 38.022253153394985 40.0 37.0 40.0 33.0 40.0 38-39 37.92944825076383 40.0 37.0 40.0 33.0 40.0 40-41 37.839030022903394 40.0 37.0 40.0 33.0 40.0 42-43 37.772111497413036 40.0 37.0 40.0 33.0 40.0 44-45 37.655847257784544 40.0 37.0 40.0 33.0 40.0 46-47 37.534631537363666 40.0 37.0 40.0 33.0 40.0 48-49 37.377072012712446 40.0 37.0 40.0 33.0 40.0 50-51 37.24525164770298 40.0 37.0 40.0 33.0 40.0 52-53 37.260602529437136 40.0 37.0 40.0 33.0 40.0 54-55 37.16073097977424 40.0 37.0 40.0 33.0 40.0 56-57 37.00675277630637 37.0 37.0 40.0 33.0 40.0 58-59 36.80713859768004 37.0 37.0 40.0 33.0 40.0 60-61 36.572806145689384 37.0 37.0 40.0 33.0 40.0 62-63 36.38910707653943 37.0 37.0 40.0 33.0 40.0 64-65 36.21250729875162 37.0 37.0 40.0 33.0 40.0 66-67 35.98476233063775 37.0 37.0 40.0 33.0 40.0 68-69 35.7585354791954 37.0 33.0 40.0 33.0 40.0 70-71 35.53357418350865 37.0 33.0 38.5 33.0 40.0 72-73 35.26390731305774 37.0 33.0 37.0 27.0 40.0 74-75 34.98837787881726 37.0 33.0 37.0 27.0 40.0 76-77 33.45270210745407 35.0 33.0 37.0 27.0 37.0 78-79 34.4982093985734 37.0 33.0 37.0 27.0 37.0 80-81 34.400361427613035 37.0 33.0 37.0 27.0 37.0 82-83 34.35146298665419 37.0 33.0 37.0 27.0 37.0 84-85 34.23643739746323 37.0 33.0 37.0 27.0 37.0 86-87 34.09474359424165 37.0 33.0 37.0 27.0 37.0 88-89 33.937064837172095 37.0 33.0 37.0 27.0 37.0 90-91 33.79270867951065 37.0 33.0 37.0 27.0 37.0 92-93 32.659109901080136 35.0 33.0 37.0 24.5 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2304 1 0.18194209426693675 2304 2 0.18705642282349544 2304 3 0.26526086980580743 2304 4 0.21402145985007337 2304 5 0.21952429015272656 2304 6 0.32842720740069353 2304 7 0.3535309434141851 2304 8 0.4377447909721184 2304 9 0.45316317908258696 2304 10-11 0.4818800065079927 2304 12-13 0.42286381670949424 2304 14-15 0.6537182701599562 2304 16-17 0.6303145969465049 2304 18-19 0.6664522767731569 2304 20-21 0.6004397459929436 2304 22-23 0.6041359785812901 2304 24-25 0.5363935894275542 2304 26-27 0.6470505200151067 2304 28-29 0.6629663762235367 2304 30-31 0.6235648411252868 2304 32-33 0.6433084421380713 2304 34-35 0.6252642072159418 2304 36-37 0.5570848488004785 2304 38-39 0.658916665473285 2304 40-41 0.5862837707623214 2304 42-43 0.6446617237924954 2304 44-45 0.5573097791249424 2304 46-47 0.5832821312988798 2304 48-49 0.5611118805469033 2304 50-51 0.6465937523836587 2304 52-53 0.666410866617376 2304 54-55 0.5055901481721179 2304 56-57 0.5976311365166538 2304 58-59 0.5539665606945761 2304 60-61 0.4880110300322116 2304 62-63 0.5042387234710404 2304 64-65 0.5418883872850415 2304 66-67 0.46480399342061673 2304 68-69 0.40306531471425444 2304 70-71 0.3872136157042192 2304 72-73 0.5253279696996103 2304 74-75 0.27758388071533346 2304 76-77 0.28865238384719305 2304 78-79 0.5254392095359179 2304 80-81 0.7576573116814274 2304 82-83 0.6312504979478959 2304 84-85 0.58427305394342 2304 86-87 0.6004112913194035 2304 88-89 0.5022790911745929 2304 90-91 0.5556430800843373 2304 92-93 0.7944874601600702 2305 1 0.19958066570566757 2305 2 0.2095717177571359 2305 3 0.26429575281787976 2305 4 0.2195898986387661 2305 5 0.270799400377733 2305 6 0.32645957826566985 2305 7 0.3290842545714838 2305 8 0.40469274977856884 2305 9 0.4381010041615738 2305 10-11 0.42279114306803933 2305 12-13 0.43685836192059924 2305 14-15 0.5553845066001486 2305 16-17 0.5300049696321025 2305 18-19 0.6040357425763005 2305 20-21 0.4627540268216279 2305 22-23 0.5564769121014805 2305 24-25 0.46562005454769917 2305 26-27 0.5025140051473684 2305 28-29 0.5501893164806617 2305 30-31 0.43447152742034234 2305 32-33 0.45599583976898117 2305 34-35 0.5924190781702166 2305 36-37 0.5185694352056629 2305 38-39 0.49199084526077286 2305 40-41 0.4821017698692742 2305 42-43 0.5730503700632354 2305 44-45 0.5637709940359414 2305 46-47 0.594669752000172 2305 48-49 0.6169457566383585 2305 50-51 0.508993746839181 2305 52-53 0.583585985474464 2305 54-55 0.5801238140626523 2305 56-57 0.6221242969958709 2305 58-59 0.6761477553543003 2305 60-61 0.6480225762973362 2305 62-63 0.6437990324473688 2305 64-65 0.6090272723660846 2305 66-67 0.6221716558979224 2305 68-69 0.5625313978576685 2305 70-71 0.4903824212840959 2305 72-73 0.5636744100244471 2305 74-75 0.5687917403328626 2305 76-77 0.5861153074340493 2305 78-79 0.7664223001963677 2305 80-81 0.9609568076607218 2305 82-83 0.7944814714529329 2305 84-85 0.6573290129563674 2305 86-87 0.6251832933982655 2305 88-89 0.5441691428530291 2305 90-91 0.5517175734090216 2305 92-93 0.6018134833913464 2306 1 0.11602969230229832 2306 2 0.1545562954485078 2306 3 0.2249343893162532 2306 4 0.17612221041152765 2306 5 0.14723285506905626 2306 6 0.27921155446539814 2306 7 0.3031187812060594 2306 8 0.3484231256041923 2306 9 0.37626124491248447 2306 10-11 0.4323778379401446 2306 12-13 0.40122717062689617 2306 14-15 0.5262297845812327 2306 16-17 0.5896739080442615 2306 18-19 0.6376129473403438 2306 20-21 0.4867154380833796 2306 22-23 0.5588695411687752 2306 24-25 0.5748711265186657 2306 26-27 0.6201787159299172 2306 28-29 0.6138854388241484 2306 30-31 0.5702932640105303 2306 32-33 0.5700923340541095 2306 34-35 0.6132086313914513 2306 36-37 0.5632295023967373 2306 38-39 0.6521553538107696 2306 40-41 0.5880891342141865 2306 42-43 0.6167225867202077 2306 44-45 0.6051927434803517 2306 46-47 0.655781165921411 2306 48-49 0.6606420299636682 2306 50-51 0.6832928656593822 2306 52-53 0.678791969701436 2306 54-55 0.6678004108547455 2306 56-57 0.7342333233265421 2306 58-59 0.6796231214921491 2306 60-61 0.5967283642479373 2306 62-63 0.6280622037416563 2306 64-65 0.5927578864382568 2306 66-67 0.5197040241849749 2306 68-69 0.5858885424561464 2306 70-71 0.47957352161193256 2306 72-73 0.5347512650370163 2306 74-75 0.5059194143040884 2306 76-77 0.544666924934198 2306 78-79 0.71522227518048 2306 80-81 0.8624911950872942 2306 82-83 0.8128791712933108 2306 84-85 0.7773422979718276 2306 86-87 0.7166692239699017 2306 88-89 0.6405940698415478 2306 90-91 0.7220622462161685 2306 92-93 0.6969818537517085 2307 1 0.13269192042205447 2307 2 0.15067667191938483 2307 3 0.23039326322867737 2307 4 0.17352255605374722 2307 5 0.14482033805890637 2307 6 0.2515872956770693 2307 7 0.2957251883126588 2307 8 0.36279121506888146 2307 9 0.423570690140302 2307 10-11 0.4367524877310984 2307 12-13 0.3743930116725238 2307 14-15 0.5504711650770773 2307 16-17 0.590504237020113 2307 18-19 0.5851017817009563 2307 20-21 0.4408008382915014 2307 22-23 0.5058871455233742 2307 24-25 0.46416882781317526 2307 26-27 0.5845568370237331 2307 28-29 0.5862481594430164 2307 30-31 0.444882262263846 2307 32-33 0.5182037066888583 2307 34-35 0.5381210278168069 2307 36-37 0.5815597903224514 2307 38-39 0.5841892832948332 2307 40-41 0.5465822552361743 2307 42-43 0.521283989013682 2307 44-45 0.5189828084216899 2307 46-47 0.6177495280616867 2307 48-49 0.508574616389609 2307 50-51 0.5680126676334822 2307 52-53 0.6103975280962928 2307 54-55 0.5971583455398601 2307 56-57 0.5159319244402383 2307 58-59 0.5215620789005726 2307 60-61 0.6187229805214471 2307 62-63 0.588604201631675 2307 64-65 0.5849342163090085 2307 66-67 0.5544345761657468 2307 68-69 0.4913314966092912 2307 70-71 0.4856158848482437 2307 72-73 0.5810777260629862 2307 74-75 0.5335157205094347 2307 76-77 0.5204672744761822 2307 78-79 0.6526978888288255 2307 80-81 0.8949834622662678 2307 82-83 0.7607756464200008 2307 84-85 0.772231529287339 2307 86-87 0.7182987222960833 2307 88-89 0.6247034561624005 2307 90-91 0.7271565691863913 2307 92-93 0.7356748735850829 2308 1 0.18697123934674664 2308 2 0.204407208450192 2308 3 0.24935481941123783 2308 4 0.18104312552416246 2308 5 0.24290486986043192 2308 6 0.2895396190427135 2308 7 0.3309366641016709 2308 8 0.40319762214850385 2308 9 0.4100555929100267 2308 10-11 0.42743821406918414 2308 12-13 0.39057238550422824 2308 14-15 0.4476936567164955 2308 16-17 0.4843143589678789 2308 18-19 0.5121236890651204 2308 20-21 0.433916049757741 2308 22-23 0.45922769534346486 2308 24-25 0.46487781752505697 2308 26-27 0.5152247042533133 2308 28-29 0.46328334641805213 2308 30-31 0.4562917188199904 2308 32-33 0.44451900535994326 2308 34-35 0.46592084207949824 2308 36-37 0.4146031860605177 2308 38-39 0.4972164037406941 2308 40-41 0.4554791496809685 2308 42-43 0.4722306769120692 2308 44-45 0.4845416432899441 2308 46-47 0.5803480904431026 2308 48-49 0.5215464902934812 2308 50-51 0.4889637553668962 2308 52-53 0.4860466053325254 2308 54-55 0.4360326491659521 2308 56-57 0.5216272048932353 2308 58-59 0.5232653029387251 2308 60-61 0.45136703264842026 2308 62-63 0.4300740660880322 2308 64-65 0.23827464441659174 2308 66-67 0.3766348352758371 2308 68-69 0.29327186810646566 2308 70-71 0.29498662908284246 2308 72-73 0.3994950814785483 2308 74-75 0.2665802487605262 2308 76-77 0.27879359509768875 2308 78-79 0.40116665635341064 2308 80-81 0.6398361009979112 2308 82-83 0.5017606430838697 2308 84-85 0.562126752490002 2308 86-87 0.5211694448813944 2308 88-89 0.3804037863894294 2308 90-91 0.4408746434503783 2308 92-93 0.49518302404121073 2309 1 0.22668304545244666 2309 2 0.22871344851298403 2309 3 0.2653850576569283 2309 4 0.182275676666535 2309 5 0.21963088695372335 2309 6 0.3298561329045242 2309 7 0.37037552173307375 2309 8 0.35490546466711237 2309 9 0.44224183675521545 2309 10-11 0.42648523395294546 2309 12-13 0.3726203578535916 2309 14-15 0.5166695909947805 2309 16-17 0.520712554701511 2309 18-19 0.5867371597512658 2309 20-21 0.505342683544086 2309 22-23 0.5292717975652224 2309 24-25 0.47403298092954316 2309 26-27 0.5411510446453249 2309 28-29 0.5723563168221659 2309 30-31 0.530782799653764 2309 32-33 0.5667019819437016 2309 34-35 0.6275805394612846 2309 36-37 0.5341726464255458 2309 38-39 0.5624139556848604 2309 40-41 0.567549525257192 2309 42-43 0.5916838261631838 2309 44-45 0.5762311919769942 2309 46-47 0.5575172033768325 2309 48-49 0.5783013431491923 2309 50-51 0.5540473624858606 2309 52-53 0.6350414530075668 2309 54-55 0.582489622900006 2309 56-57 0.6064495073595069 2309 58-59 0.6411717972341577 2309 60-61 0.6721547616039203 2309 62-63 0.6989403777750525 2309 64-65 0.5845604644113394 2309 66-67 0.6049407347051314 2309 68-69 0.5609128497797542 2309 70-71 0.5537861028691395 2309 72-73 0.6609369241589746 2309 74-75 0.5802017123267476 2309 76-77 0.5553799249973395 2309 78-79 0.730160596127547 2309 80-81 0.9350443742422101 2309 82-83 0.7915486658463422 2309 84-85 0.7600975947085473 2309 86-87 0.6699721213847027 2309 88-89 0.6657982859953719 2309 90-91 0.6908982388384644 2309 92-93 0.7603952294001814 2310 1 0.18187818196985717 2310 2 0.17693783472368096 2310 3 0.2482380962320505 2310 4 0.16336210282423025 2310 5 0.24023193489698969 2310 6 0.30288662919534914 2310 7 0.32584678088019814 2310 8 0.34757671009919733 2310 9 0.39919309732045605 2310 10-11 0.3995367663572864 2310 12-13 0.3691634900865637 2310 14-15 0.4725417034445911 2310 16-17 0.3911667156621377 2310 18-19 0.5014745255232782 2310 20-21 0.42239179691411977 2310 22-23 0.42053090184715103 2310 24-25 0.38330001104554157 2310 26-27 0.3978521608396264 2310 28-29 0.4449143315798807 2310 30-31 0.4402074956165052 2310 32-33 0.4906527349725778 2310 34-35 0.3965389662295067 2310 36-37 0.4211661245310552 2310 38-39 0.4620257126152154 2310 40-41 0.4423956681652754 2310 42-43 0.45007233344431796 2310 44-45 0.4442520528438081 2310 46-47 0.4456667732622037 2310 48-49 0.4237250183480441 2310 50-51 0.43117381042347347 2310 52-53 0.47226562015768536 2310 54-55 0.4639643279718655 2310 56-57 0.5058242550652281 2310 58-59 0.5210464772817076 2310 60-61 0.5202648250259756 2310 62-63 0.5026061229781433 2310 64-65 0.44256066891859547 2310 66-67 0.43481635693571263 2310 68-69 0.44990061620880795 2310 70-71 0.4329979573220868 2310 72-73 0.5432778889527157 2310 74-75 0.4556238210982215 2310 76-77 0.45388468339216104 2310 78-79 0.6233095206576706 2310 80-81 0.8745338128355726 2310 82-83 0.6480173682810175 2310 84-85 0.6990480406180808 2310 86-87 0.6869055401986373 2310 88-89 0.5830905049067425 2310 90-91 0.6105682169843476 2310 92-93 0.6309888436744018 2311 1 0.18275444532201135 2311 2 0.17471288543173813 2311 3 0.23157921025506312 2311 4 0.16505300729680528 2311 5 0.2623628533611253 2311 6 0.3329104093070825 2311 7 0.36134911832773753 2311 8 0.3780159939454535 2311 9 0.39637061779431804 2311 10-11 0.3649727738007087 2311 12-13 0.3353639573825191 2311 14-15 0.3715389015027242 2311 16-17 0.40908490438253864 2311 18-19 0.47365118457621236 2311 20-21 0.3788537811269208 2311 22-23 0.3476108042817643 2311 24-25 0.3216741113440875 2311 26-27 0.2525688002237345 2311 28-29 0.3752590735299748 2311 30-31 0.3632842768690452 2311 32-33 0.3693990793980717 2311 34-35 0.4058832025506476 2311 36-37 0.4082535408030452 2311 38-39 0.40623778058596827 2311 40-41 0.40873491711049326 2311 42-43 0.3260461691923666 2311 44-45 0.3306130972677934 2311 46-47 0.3711212908456858 2311 48-49 0.35322331575464716 2311 50-51 0.3250651229891446 2311 52-53 0.2978986253980125 2311 54-55 0.37168354355049615 2311 56-57 0.38071657791365254 2311 58-59 0.38116385227348815 2311 60-61 0.48034119627070737 2311 62-63 0.4473331571441648 2311 64-65 0.3842545670721975 2311 66-67 0.4197448556808112 2311 68-69 0.3631163146131513 2311 70-71 0.36766876153072303 2311 72-73 0.36210018180089065 2311 74-75 0.32494610154000014 2311 76-77 0.3383035684684472 2311 78-79 0.5063482125337657 2311 80-81 0.6747228991306571 2311 82-83 0.5787001505063003 2311 84-85 0.5347917818004504 2311 86-87 0.4887739741328403 2311 88-89 0.470184961229954 2311 90-91 0.42164676540208745 2311 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1114 46-47 0.17664563467913297 1114 48-49 0.30943418627440167 1114 50-51 0.2746712535784539 1114 52-53 0.023401903088583254 1114 54-55 0.05917675973571335 1114 56-57 0.269719101929347 1114 58-59 0.3215181970822698 1114 60-61 0.43318725430740557 1114 62-63 0.4804865146475876 1114 64-65 0.4108284193777507 1114 66-67 0.379227468449308 1114 68-69 0.4420176160418734 1114 70-71 0.47342083184118167 1114 72-73 0.3979481578545432 1114 74-75 0.42616363058399287 1114 76-77 0.44613427173233333 1114 78-79 0.36071175337734473 1114 80-81 0.46657479271662083 1114 82-83 0.3403703030813361 1114 84-85 0.22888068577359633 1114 86-87 0.35904715752713656 1114 88-89 0.2614339235146659 1114 90-91 0.2556667716160774 1114 92-93 0.39361855434796666 1115 1 -0.3501832499859532 1115 2 -0.5607052684334555 1115 3 -1.4237249524467472 1115 4 -0.6162720329324287 1115 5 -0.3267293277075538 1115 6 -0.010617566389839794 1115 7 0.041720273846927114 1115 8 0.04853521875125466 1115 9 -1.0740871175467959 1115 10-11 -0.7024882488130331 1115 12-13 -0.13101343475960903 1115 14-15 -0.18295367589236378 1115 16-17 -0.10362158283250267 1115 18-19 -0.0030587097323575563 1115 20-21 -0.06092937985974345 1115 22-23 -0.052406938147044 1115 24-25 -0.13401585566486318 1115 26-27 -0.030698227712150583 1115 28-29 -0.13449938501219094 1115 30-31 -0.04203379009719299 1115 32-33 -0.024677630336050527 1115 34-35 -0.100184328807714 1115 36-37 -0.18596753233319419 1115 38-39 -0.0882214444281999 1115 40-41 -0.10919376334173592 1115 42-43 0.02889914648955738 1115 44-45 -0.12360640875967732 1115 46-47 0.04742648565509455 1115 48-49 -0.1939524506721355 1115 50-51 0.03745585085118108 1115 52-53 0.14903904868400986 1115 54-55 0.12113601579904554 1115 56-57 0.00821186395064899 1115 58-59 -0.07129651021617178 1115 60-61 -0.022415844886673142 1115 62-63 0.16554401764840065 1115 64-65 0.12186420671864795 1115 66-67 0.11733057840801564 1115 68-69 0.2257111640716971 1115 70-71 0.2832434880792789 1115 72-73 0.34122788586923036 1115 74-75 0.22631412078579416 1115 76-77 0.1684030824637759 1115 78-79 0.17516962250562784 1115 80-81 0.242142350003256 1115 82-83 -0.0024778936693152787 1115 84-85 0.10323226284594966 1115 86-87 0.09153751934032073 1115 88-89 0.20802247836083865 1115 90-91 0.1026908942758098 1115 92-93 0.10514124899691524 1116 1 -1.5074400985451177 1116 2 -0.9780699871305991 1116 3 -0.9130485052887032 1116 4 -0.6198391593436412 1116 5 -0.2164728919856742 1116 6 -0.04280777112033718 1116 7 -1.5661772899238926 1116 8 -1.1272739942398857 1116 9 -1.0923144322000695 1116 10-11 -1.7947118581929686 1116 12-13 -0.6583969464211634 1116 14-15 -1.8605412513186437 1116 16-17 -1.7510150176810697 1116 18-19 -0.6738418240681909 1116 20-21 -0.33946964257859946 1116 22-23 -0.14402780902209855 1116 24-25 -0.6459377658771572 1116 26-27 -0.6113034981659453 1116 28-29 -0.8182569636307306 1116 30-31 -0.3040083556286888 1116 32-33 -0.9703023766085224 1116 34-35 -0.8333720182828799 1116 36-37 -0.9876468867114596 1116 38-39 -0.5245084786085954 1116 40-41 -0.18478348829658842 1116 42-43 -0.15751553489294423 1116 44-45 -0.3815102124442902 1116 46-47 -0.37099350969249656 1116 48-49 -0.6146039223781301 1116 50-51 -0.41924795773248746 1116 52-53 -0.1288911792175682 1116 54-55 0.0022033535008105787 1116 56-57 0.07038531182541163 1116 58-59 -0.036511755280017155 1116 60-61 0.0440428537493176 1116 62-63 0.07362074473099511 1116 64-65 -0.02033122067270199 1116 66-67 -0.1871502274596324 1116 68-69 0.0252587736428751 1116 70-71 0.11314785047689213 1116 72-73 0.10526060965561612 1116 74-75 0.12337737439509766 1116 76-77 0.1727146915686717 1116 78-79 0.017654070360890728 1116 80-81 0.13575638202867424 1116 82-83 -0.36511726740491923 1116 84-85 -0.15787078935920817 1116 86-87 -0.0058838653808237495 1116 88-89 -0.0720199262097978 1116 90-91 -0.03016034343442442 1116 92-93 -0.21516532632531238 2301 1 -0.27061502086002776 2301 2 -0.15741804103160462 2301 3 0.047332016464309845 2301 4 -0.012250819563874416 2301 5 0.04357875854776694 2301 6 0.10811530171123707 2301 7 0.12220130767988024 2301 8 0.13912057560023783 2301 9 -0.31843385524303613 2301 10-11 -1.1185878274587395 2301 12-13 -0.5370591906133768 2301 14-15 -0.7377889135025413 2301 16-17 -0.49408909640474974 2301 18-19 -0.18468483176167894 2301 20-21 -0.7329911229423232 2301 22-23 -0.30888872279806634 2301 24-25 -0.23853678444096715 2301 26-27 -0.2085562456011587 2301 28-29 -0.5426892223127879 2301 30-31 -0.4621337963283523 2301 32-33 -0.5526298016147209 2301 34-35 -0.6769129728213485 2301 36-37 -0.42681341654445504 2301 38-39 -0.3276584142915766 2301 40-41 -0.2573326137371623 2301 42-43 -0.26950892896211087 2301 44-45 -0.22294442313179275 2301 46-47 -0.7201568552584661 2301 48-49 -0.8605056932377266 2301 50-51 -0.6059385707315101 2301 52-53 -0.32497611557240447 2301 54-55 -0.3062945140870781 2301 56-57 -0.27994474396859204 2301 58-59 -0.3418886490250159 2301 60-61 -0.2262166962101304 2301 62-63 -0.20040527166765543 2301 64-65 -0.2879842199089637 2301 66-67 -0.328396508167792 2301 68-69 -0.27175993634182305 2301 70-71 -0.23539759961641948 2301 72-73 -0.2179229184613405 2301 74-75 -0.12879828528715365 2301 76-77 -0.2683519941584791 2301 78-79 -0.29517958800590804 2301 80-81 -0.13057889560084845 2301 82-83 -0.23682857393033885 2301 84-85 -0.33191397915677356 2301 86-87 -0.36359458591306293 2301 88-89 -0.3369315698460795 2301 90-91 -0.33812482136275435 2301 92-93 -0.4068382675976352 2302 1 -0.3114732640927045 2302 2 -0.20740305638331336 2302 3 0.011583193573805772 2302 4 -0.06495166044800982 2302 5 0.005432626467452906 2302 6 0.027956457811434632 2302 7 -0.20056986729268544 2302 8 -0.4010576672314343 2302 9 -0.02588136065274682 2302 10-11 -0.19966885589798977 2302 12-13 -0.10998806104216641 2302 14-15 -0.24335567926830493 2302 16-17 -0.7004489275709602 2302 18-19 -0.21539435365757953 2302 20-21 -0.2555904508096134 2302 22-23 -0.733420529816776 2302 24-25 -0.738850459537403 2302 26-27 -0.5536474239765639 2302 28-29 -0.35146991831859253 2302 30-31 -0.2425628775347377 2302 32-33 -0.2207158512434617 2302 34-35 -0.1734206709474293 2302 36-37 -0.1759366782181715 2302 38-39 -0.143821059982713 2302 40-41 -0.16457342258296848 2302 42-43 -0.16192649717647356 2302 44-45 -0.19919072005839666 2302 46-47 -0.13257643372902805 2302 48-49 -0.1439324472399477 2302 50-51 -0.13580284140320487 2302 52-53 -0.47647866370097347 2302 54-55 -0.2662601711253316 2302 56-57 -0.14717763702399367 2302 58-59 -0.33260494478267333 2302 60-61 -0.2474514282936724 2302 62-63 -0.1920960017217297 2302 64-65 -0.16857953682257687 2302 66-67 -0.04258838180313518 2302 68-69 -0.031047260932041354 2302 70-71 0.005831468437108356 2302 72-73 0.003849952545280644 2302 74-75 0.050924839984553216 2302 76-77 0.04649862300023244 2302 78-79 -0.06875829239726272 2302 80-81 0.05140177054362738 2302 82-83 -0.11678898702141538 2302 84-85 -0.2440466804018797 2302 86-87 -0.12379596831619466 2302 88-89 -0.17816009894097107 2302 90-91 -0.16622681905059977 2302 92-93 -0.38483204005550675 2303 1 -0.710635644655742 2303 2 -0.5647440083675015 2303 3 -0.6527072243223486 2303 4 -0.5238819477507235 2303 5 -0.1954688224920531 2303 6 -0.04937086878697272 2303 7 -0.014071343256915725 2303 8 -0.1368574559152549 2303 9 -2.976999331494312E-4 2303 10-11 -0.23519548269602097 2303 12-13 -0.2540410656202994 2303 14-15 -0.5249010017415401 2303 16-17 -0.3299380596572732 2303 18-19 -0.24807218255261176 2303 20-21 -0.32809776335554375 2303 22-23 -0.42603657565725683 2303 24-25 -0.37220159830977906 2303 26-27 -0.3737511921275285 2303 28-29 -0.6547301462885144 2303 30-31 -0.541434527751349 2303 32-33 -0.32225847491788073 2303 34-35 -0.2235900346059836 2303 36-37 -0.19782125743739698 2303 38-39 -0.17132152573309156 2303 40-41 -0.25618356941778586 2303 42-43 -0.262353433854571 2303 44-45 -0.36943918645683027 2303 46-47 -0.16932705163495143 2303 48-49 -0.17909836191384443 2303 50-51 -0.1159366614928814 2303 52-53 -0.12203478209192298 2303 54-55 -0.1910679284765493 2303 56-57 -0.17089438802160828 2303 58-59 -0.6234531171775402 2303 60-61 -0.5337960620512519 2303 62-63 -0.4370268235613324 2303 64-65 -0.3856137990363351 2303 66-67 -0.3997100090450303 2303 68-69 -0.26997805276029396 2303 70-71 -0.22596741588783686 2303 72-73 -0.25064465612058484 2303 74-75 -0.16706032815059046 2303 76-77 -0.13641266105196337 2303 78-79 -0.2643431163946417 2303 80-81 -0.1731725764089873 2303 82-83 -0.22659141798296645 2303 84-85 -0.24777890010633286 2303 86-87 -0.19576167394991728 2303 88-89 -0.270152760801075 2303 90-91 -0.32919814332357333 2303 92-93 -0.5967634006723053 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 3.0 12 29.0 13 124.0 14 406.0 15 990.0 16 2560.0 17 5369.0 18 9891.0 19 16732.0 20 25875.0 21 39382.0 22 57335.0 23 80356.0 24 107542.0 25 140326.0 26 181342.0 27 231327.0 28 296718.0 29 379968.0 30 489135.0 31 633969.0 32 837776.0 33 1139286.0 34 1619186.0 35 2497686.0 36 4430024.0 37 9160824.0 38 1.1163401E7 39 256163.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.8082176078291 24.202342766532624 10.722339329160485 24.267100296477793 2 15.896034656061286 22.527410144077017 38.049947511703294 23.5266076881584 3 15.493862805632041 26.88304719125587 31.634343794114965 25.988746208997114 4 11.646763436669675 17.17026105347085 41.729541885412274 29.4534336244472 5 11.863647812078467 38.096889082582194 37.98097878322644 12.058484322112893 6 28.837862429721504 41.58000215715865 18.142497072659978 11.439638340459865 7 26.26577022899783 32.607349260847755 25.72090721596785 15.405973294186564 8 23.38851048550092 36.60068715501954 23.2060158102098 16.804786549269746 9 22.76479817639038 14.840577041714278 26.41505554742693 35.979569234468414 10-11 22.434016593318738 27.038085979042663 31.565906373753 18.961991053885598 12-13 21.50045530483829 25.912032596643343 31.87866775396298 20.70884434455539 14-15 19.393610633336692 27.328700392376863 27.550850459502602 25.726838514783847 16-17 19.74365932323555 31.81815371477097 28.55832076026175 19.879866201731726 18-19 19.892605921607576 30.51941374746677 30.095760745427885 19.492219585497764 20-21 20.116435987940264 30.055250717346997 29.93502431862241 19.893288976090325 22-23 20.254541628167345 29.805098423464987 29.847326141876025 20.093033806491647 24-25 19.811375538310553 29.667369006476367 30.211823122781766 20.30943233243131 26-27 20.081522443344493 30.24130072861601 29.86146734481336 19.81570948322614 28-29 20.126896581429413 30.068080375516587 29.861001808592746 19.94402123446125 30-31 19.898535774655553 29.882121813277546 30.092093904957885 20.12724850710902 32-33 20.097028607967548 30.181960071658402 29.88769113905814 19.833320181315905 34-35 19.957750777362946 29.847596940120404 30.03759064353193 20.157061638984715 36-37 19.896375146193282 30.066743078382586 30.09951504558855 19.937366729835585 38-39 19.980187448457354 30.03954138349551 30.095106933138727 19.885164234908412 40-41 19.832651325496347 29.949018404227388 30.21938637783949 19.998943892436778 42-43 19.861513240571004 29.97901895952641 30.220280364896094 19.93918743500649 44-45 19.820554328342215 29.835683610158682 30.206750679511597 20.137011381987506 46-47 19.757525479613957 29.831059674632808 30.36650867261359 20.044906173139644 48-49 19.812926436638016 30.061282968954746 30.20455025197686 19.921240342430387 50-51 19.69964423625368 29.992527721814827 30.323996727805397 19.983831314126096 52-53 19.762396904347426 29.839852569657175 30.428029517134565 19.969721008860837 54-55 19.684026312950916 29.970608214070854 30.33433356299582 20.01103190998241 56-57 19.66198265792271 29.94167956429162 30.267270473170534 20.129067304615138 58-59 19.751367366051987 29.965667039663547 30.196745131166015 20.086220463118455 60-61 19.600505181454007 29.88418109289231 30.351848779322292 20.163464946331395 62-63 19.574428555312014 29.976071090873795 30.226153007186614 20.22334734662758 64-65 19.638699126085175 29.88840695591976 30.254028267690302 20.218865650304767 66-67 19.616832269466457 29.818461190934325 30.208214940357486 20.35649159924173 68-69 19.602709930153082 29.980074861930817 30.113001435102245 20.304213772813853 70-71 19.66659917982006 29.913963897449815 30.091595991844112 20.327840930886016 72-73 19.59639632850651 29.881548926293185 30.04374438732331 20.478310357876992 74-75 19.566288710030626 29.948385490353836 30.08543205650596 20.39989374310957 76-77 19.58925986009101 29.910363563021043 30.056343758032956 20.444032818854993 78-79 19.69045709185528 29.857735286716924 29.993475117365552 20.458332504062245 80-81 19.632620067606517 29.90843189255093 29.918591896323544 20.540356143519013 82-83 19.587722936246138 29.892731009428463 29.995854455811617 20.523691598513786 84-85 19.55698465556008 29.882066590535462 30.027824805800385 20.533123948104073 86-87 19.44118233793223 29.805793629404135 30.107170741605977 20.645853291057655 88-89 19.553650494895326 29.79116498160847 30.024414045018737 20.63077047847747 90-91 19.605628072747248 29.821613881568076 29.984633342372135 20.588124703312538 92-93 19.599500012905708 29.7164316434942 29.968976748943447 20.715091594656638 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1.0 1 1.0 2 2.5 3 7.5 4 19.0 5 56.0 6 139.0 7 291.0 8 581.0 9 1016.0 10 1710.0 11 2746.0 12 4377.5 13 6996.5 14 8569.0 15 10718.5 16 16450.0 17 25931.0 18 40534.0 19 62175.5 20 93520.0 21 135442.0 22 191129.5 23 264931.5 24 353298.0 25 456729.0 26 577725.5 27 713174.5 28 856631.5 29 928942.0 30 995948.5 31 1124775.5 32 1241875.0 33 1344300.5 34 1428795.5 35 1492815.0 36 1529702.0 37 1537988.0 38 1522615.0 39 1487316.5 40 1442864.5 41 1387805.0 42 1324554.5 43 1293767.0 44 1257081.5 45 1166503.0 46 1070835.0 47 981986.0 48 891805.0 49 807935.0 50 726420.5 51 649815.5 52 577288.0 53 508690.0 54 448145.5 55 394739.5 56 348997.5 57 328041.0 58 311119.5 59 277623.0 60 243710.0 61 212446.0 62 185468.5 63 161156.5 64 140788.0 65 123401.5 66 107304.0 67 93050.5 68 79091.5 69 65032.0 70 52320.5 71 46144.0 72 42025.0 73 34317.5 74 28475.5 75 24582.5 76 22171.5 77 20003.0 78 16601.0 79 13725.0 80 11161.0 81 8688.5 82 6938.5 83 5205.0 84 3678.5 85 2297.5 86 1680.0 87 1395.5 88 931.0 89 533.5 90 244.5 91 135.0 92 74.0 93 40.5 94 25.0 95 17.0 96 11.0 97 5.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20309299631644157 2 0.0 3 1.774952264137983E-5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 5.91650754712661E-6 22-23 2.958253773563305E-4 24-25 1.5087094245172855E-4 26-27 0.002987836311298938 28-29 0.001101949530652331 30-31 5.502352018827748E-4 32-33 8.6824748254083E-4 34-35 0.001480606013668434 36-37 0.0013179020561224523 38-39 0.0012040092858402651 40-41 0.00103834707452072 42-43 0.0010353888207471568 44-45 0.0011921762707460119 46-47 3.9344775188391955E-4 48-49 3.7126084858219474E-4 50-51 3.3428267641265346E-4 52-53 0.013943729161690637 54-55 0.06158492705804088 56-57 0.09754398080258964 58-59 0.11778435312130976 60-61 0.1265762833363399 62-63 0.13367609239289183 64-65 0.13481797834948725 66-67 0.131148264543382 68-69 0.13049005307876416 70-71 0.13034361951697276 72-73 0.1324306675542217 74-75 0.130260788411313 76-77 0.1312044713650797 78-79 0.11576830317462637 80-81 0.10073889487803799 82-83 0.08194954603525068 84-85 0.0602936492858805 86-87 0.036998879945956256 88-89 0.018864784314013193 90-91 0.0039048949811035624 92-93 0.002524869595736281 >>END_MODULE >>Sequence Length Distribution pass #Length Count 93 3.3803726E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.78405511767535 #Duplication Level Percentage of deduplicated Percentage of total 1 88.53258185707558 65.32292899452571 2 7.198142066980657 10.62216222029917 3 1.5790979226951156 3.4953674449302925 4 0.795282777630088 2.3471675319518543 5 0.4230728542056293 1.560801539675019 6 0.2764403327631348 1.223813324960619 7 0.19828680824301603 1.0241283351957433 8 0.1481281124355964 0.8743594249940206 9 0.12416016634546842 0.8244936501348403 >10 0.6729249395083031 8.946280480499617 >50 0.04082259032804363 2.0766265875354133 >100 0.010601011185867168 1.3560692623487614 >500 3.907389665232776E-4 0.17443787696174035 >1k 5.382084674936193E-5 0.061785345454300124 >5k 9.715142908930892E-6 0.053102875751142364 >10k+ 4.2856473258778486E-6 0.03647510478183887 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 5.91650754712661E-6 2.958253773563305E-6 0.0 2 5.91650754712661E-6 2.958253773563305E-6 2.958253773563305E-6 3 1.183301509425322E-5 5.91650754712661E-6 5.91650754712661E-6 4 1.183301509425322E-5 5.91650754712661E-6 5.91650754712661E-6 5 3.549904528275966E-5 5.91650754712661E-6 5.91650754712661E-6 6 4.437380660344957E-5 5.91650754712661E-6 1.183301509425322E-5 7 4.437380660344957E-5 8.874761320689915E-6 2.0707776414943134E-5 8 5.91650754712661E-5 8.874761320689915E-6 2.0707776414943134E-5 9 6.21233292448294E-5 8.874761320689915E-6 3.549904528275966E-5 10-11 6.656070990517436E-5 1.0353888207471567E-5 7.099809056551932E-5 12-13 9.170586698046246E-5 1.922864952816148E-5 7.839372499942758E-5 14-15 1.0353888207471567E-4 2.0707776414943134E-5 8.874761320689915E-5 16-17 1.242466584896588E-4 2.0707776414943134E-5 1.212884047160955E-4 18-19 1.3903792735747532E-4 2.958253773563305E-5 1.8193260707414323E-4 20-21 1.4939181556494687E-4 3.549904528275966E-5 2.8103410848851396E-4 22-23 1.5826657688563681E-4 3.697817216954131E-5 4.55571081128749E-4 24-25 1.7009959197989002E-4 5.620682169770279E-5 0.001013201917445432 26-27 1.804534801873616E-4 5.620682169770279E-5 0.005108904266943827 28-29 1.9672387594195977E-4 6.21233292448294E-5 0.026295917793204216 30-31 2.174316523569029E-4 6.656070990517436E-5 0.04540919542419673 32-33 2.3370204811150108E-4 7.543547122586426E-5 0.06333177591133002 34-35 2.484933169793176E-4 8.431023254655418E-5 0.0944747925125177 36-37 2.6032633207357086E-4 8.874761320689914E-5 0.16345831225824042 38-39 2.6920109339426075E-4 9.02267400936808E-5 0.27805958431919603 40-41 2.8103410848851396E-4 9.614324764080741E-5 0.4086265519960729 42-43 2.899088698092039E-4 1.0205975518793402E-4 0.5399271666087934 44-45 3.0913751933736534E-4 1.0501800896149733E-4 0.6967397617647237 46-47 3.2836616886552683E-4 1.0945538962184228E-4 0.8828775265779873 48-49 3.5351132594081496E-4 1.1537189716896888E-4 1.0681174613709743 50-51 3.727399754689764E-4 1.212884047160955E-4 1.25076596585832 52-53 3.83093863676448E-4 1.212884047160955E-4 1.4254168312688371 54-55 4.008433863178278E-4 1.2276753160287714E-4 1.6080002541731642 56-57 4.200720358459893E-4 1.316422929235671E-4 1.8120798281230892 58-59 4.452171929212774E-4 1.4051705424425698E-4 2.0188040809465795 60-61 5.147361566000151E-4 1.4495443490460192E-4 2.2209681855781223 62-63 5.265691716942682E-4 1.4791268867816524E-4 2.4133567406149252 64-65 5.457978212224297E-4 1.5235006933851018E-4 2.602730835056467 66-67 5.546725825431196E-4 1.5826657688563681E-4 2.8071742742205403 68-69 5.709429782977179E-4 1.6270395754598179E-4 3.017383349989288 70-71 5.931298815994426E-4 1.671413382063267E-4 3.222371107847697 72-73 6.12358531127604E-4 1.7305784575345333E-4 3.420050499758518 74-75 6.21233292448294E-4 1.8193260707414325E-4 3.6117512607929667 76-77 6.404619419764555E-4 1.8489086084770654E-4 3.8100385738542553 78-79 6.567323377310537E-4 1.8932824150805151E-4 4.018055583576793 80-81 6.789192410327784E-4 1.9820300282874143E-4 4.225834453870558 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACTG 14425 0.0 15.5213785 5 GTATAAG 15445 0.0 13.313373 1 GTATAGG 11260 0.0 13.270575 1 GTATTAG 15330 0.0 12.986977 1 GTATATA 29390 0.0 12.881137 1 CTATACT 17425 0.0 12.67447 4 GTGTAGG 11580 0.0 12.339549 1 TACACCG 6125 0.0 12.279449 5 GTGTAAG 10885 0.0 12.046809 1 GTATAGA 17695 0.0 11.940573 1 TACACTG 11330 0.0 11.818427 5 TATGCCG 16120 0.0 11.695629 86-87 CTTACAC 10710 0.0 11.690738 3 TATACCG 5175 0.0 11.677324 5 TCTATAC 18475 0.0 11.671754 3 TTATACT 25255 0.0 11.61971 4 TATACAG 14105 0.0 11.496738 5 ATACCGT 6240 0.0 11.286761 6 TACACAG 9795 0.0 11.229359 5 ATGCCGT 13800 0.0 11.21942 86-87 >>END_MODULE