FastQCFastQC Report
Wed 5 Nov 2014
Kamla_M3_TCCTGAGC-TATCCTCT_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameKamla_M3_TCCTGAGC-TATCCTCT_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33803726
Sequences flagged as poor quality0
Sequence length93
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG144250.015.52137855
GTATAAG154450.013.3133731
GTATAGG112600.013.2705751
GTATTAG153300.012.9869771
GTATATA293900.012.8811371
CTATACT174250.012.674474
GTGTAGG115800.012.3395491
TACACCG61250.012.2794495
GTGTAAG108850.012.0468091
GTATAGA176950.011.9405731
TACACTG113300.011.8184275
TATGCCG161200.011.69562986-87
CTTACAC107100.011.6907383
TATACCG51750.011.6773245
TCTATAC184750.011.6717543
TTATACT252550.011.619714
TATACAG141050.011.4967385
ATACCGT62400.011.2867616
TACACAG97950.011.2293595
ATGCCGT138000.011.2194286-87
Produced by FastQC (version 0.11.2)