FastQCFastQC Report
Wed 5 Nov 2014
DP6A_TAGGCATG-TAGATCGC_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDP6A_TAGGCATG-TAGATCGC_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53830827
Sequences flagged as poor quality0
Sequence length93
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG235150.016.6219625
CTATACT286550.015.6128634
TATACAG232600.014.3742165
TACACCG89700.014.2017795
TCTATAC293350.013.7540143
TACACAG141850.013.7007945
CTTACAC161350.013.5809183
TACACTG158600.013.5696795
GTGTAGG179900.013.2115371
TATACCG97500.012.9764525
GTATAGA287450.012.4631971
GTATAAG253250.012.4617911
ACTGCGC55250.012.3547778
TAGACTG164500.012.3165085
GTATAGG199650.012.2970711
GTATATA469750.012.2598451
GTATTAG252800.012.2084651
TATACTA365650.012.1640335
TTATACT403000.011.9968534
TATGCCG207250.011.73986486-87
Produced by FastQC (version 0.11.2)