FastQCFastQC Report
Wed 5 Nov 2014
DP4A_TAGGCATG-AGAGTAGA_L008_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDP4A_TAGGCATG-AGAGTAGA_L008_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53263242
Sequences flagged as poor quality0
Sequence length93
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG233800.016.1052685
CTATACT268450.014.5302674
TACACCG90550.014.0693425
CTTACAC169650.013.9456223
GTATTAG243050.013.6709271
GTATATA473700.013.5880951
TATACAG230350.013.5528565
GTGTAGG180200.013.4190031
GTATAGA287400.013.36208251
GTATAGG198050.013.1318691
TATACCG88850.013.0172375
TTATACT415450.012.9373194
TCTATAC285350.012.893933
GTATAAG246100.012.7946151
TACACTG165600.012.6818145
CCTATAC195700.012.5337533
GTCCTAG204000.012.3651121
GTATTAT392800.012.2235641
GTGTAAG172450.012.2062551
ACTGTGC150350.012.1881978
Produced by FastQC (version 0.11.2)