##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename DP2A_AGGCAGAA-TATCCTCT_L007_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 217865 Sequences flagged as poor quality 0 Sequence length 93 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.090179698437105 33.0 33.0 33.0 33.0 33.0 2 32.08808206917128 33.0 33.0 33.0 33.0 33.0 3 32.10410116356459 33.0 33.0 33.0 33.0 33.0 4 35.81202579579097 37.0 37.0 37.0 33.0 37.0 5 35.81675349413628 37.0 37.0 37.0 33.0 37.0 6 35.88261538108462 37.0 37.0 37.0 33.0 37.0 7 35.875280563651806 37.0 37.0 37.0 33.0 37.0 8 35.971927569825354 37.0 37.0 37.0 33.0 37.0 9 35.96508847221904 37.0 37.0 37.0 33.0 37.0 10-11 36.055171780689875 37.0 37.0 37.0 35.0 37.0 12-13 36.06028733389943 37.0 37.0 37.0 37.0 37.0 14-15 38.06572648199573 40.0 37.0 40.0 37.0 40.0 16-17 38.072365914671934 40.0 37.0 40.0 37.0 40.0 18-19 38.08100658664769 40.0 37.0 40.0 35.0 40.0 20-21 37.964411447455994 40.0 37.0 40.0 33.0 40.0 22-23 37.884322860487 40.0 37.0 40.0 33.0 40.0 24-25 37.78504349023478 40.0 37.0 40.0 33.0 40.0 26-27 37.687981089206616 40.0 37.0 40.0 33.0 40.0 28-29 37.68287471599385 40.0 37.0 40.0 33.0 40.0 30-31 37.53694489706929 40.0 37.0 40.0 33.0 40.0 32-33 37.38277373602919 40.0 37.0 40.0 33.0 40.0 34-35 37.311341886030334 40.0 37.0 40.0 33.0 40.0 36-37 37.25655796020472 40.0 37.0 40.0 33.0 40.0 38-39 37.108066922176576 40.0 37.0 40.0 33.0 40.0 40-41 37.052057466779885 40.0 37.0 40.0 33.0 40.0 42-43 37.09248387763064 40.0 37.0 40.0 33.0 40.0 44-45 36.95827002960549 40.0 37.0 40.0 33.0 40.0 46-47 36.77440387395865 40.0 37.0 40.0 33.0 40.0 48-49 36.57947811718266 40.0 37.0 40.0 33.0 40.0 50-51 35.51408211507126 37.0 35.0 38.5 30.0 40.0 52-53 35.65886213939825 37.0 37.0 38.5 30.0 40.0 54-55 36.383283225850874 37.0 37.0 40.0 33.0 40.0 56-57 36.431611318936035 37.0 37.0 40.0 33.0 40.0 58-59 36.25412755605535 37.0 37.0 40.0 33.0 40.0 60-61 36.447299933445024 37.0 37.0 40.0 33.0 40.0 62-63 36.36960503063824 37.0 37.0 40.0 33.0 40.0 64-65 36.21283822550662 37.0 37.0 40.0 33.0 40.0 66-67 36.00141830950359 37.0 37.0 40.0 33.0 40.0 68-69 35.7782502926124 37.0 37.0 40.0 30.0 40.0 70-71 35.537495696876505 37.0 37.0 40.0 27.0 40.0 72-73 35.270617125284005 37.0 33.0 38.5 27.0 40.0 74-75 35.06333050283432 37.0 33.0 37.0 27.0 40.0 76-77 34.6664562917403 37.0 33.0 37.0 27.0 40.0 78-79 34.40839740206091 37.0 33.0 37.0 27.0 40.0 80-81 34.21524108966562 37.0 33.0 37.0 27.0 37.0 82-83 34.03264177357538 37.0 33.0 37.0 27.0 37.0 84-85 33.781428866499894 37.0 33.0 37.0 27.0 37.0 86-87 33.62012025795791 37.0 33.0 37.0 27.0 37.0 88-89 33.40793610722236 37.0 33.0 37.0 27.0 37.0 90-91 33.211055011130746 37.0 33.0 37.0 27.0 37.0 92-93 32.24873889794139 35.0 33.0 37.0 24.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1201 1 -0.12319107620027836 1201 2 -0.0827164133182805 1201 3 -0.045579872680455935 1201 4 -0.2945907528573528 1201 5 -0.12090951997548416 1201 6 -0.17143486794494578 1201 7 0.005783326457134308 1201 8 0.047720789451609846 1201 9 0.11021298650665301 1201 10-11 0.23287643257945234 1201 12-13 0.2146815132491895 1201 14-15 0.3830836651607612 1201 16-17 0.5626595950301194 1201 18-19 0.5420871150017774 1201 20-21 0.5974638003290025 1201 22-23 0.646172444494745 1201 24-25 0.76763363955709 1201 26-27 0.8134766584710675 1201 28-29 0.7701524850100725 1201 30-31 0.5925865171539257 1201 32-33 0.6058403309473732 1201 34-35 0.6413599950789433 1201 36-37 0.5716042115893458 1201 38-39 0.5426627676517626 1201 40-41 0.5068897225422972 1201 42-43 0.6231817223539551 1201 44-45 0.6059941250363252 1201 46-47 0.5125161845007113 1201 48-49 0.8141118315471729 1201 50-51 0.6083644119233185 1201 52-53 0.3107932167067773 1201 54-55 0.5204820798508862 1201 56-57 0.49093326436462803 1201 58-59 0.5775994374974189 1201 60-61 0.6607012303024931 1201 62-63 0.5700722084124052 1201 64-65 0.5924234199665293 1201 66-67 0.678154817389796 1201 68-69 0.7354490489773795 1201 70-71 0.7481223958166154 1201 72-73 0.7774015520705788 1201 74-75 0.5838157988455777 1201 76-77 0.4083597209381793 1201 78-79 0.4464321534884874 1201 80-81 0.5326922324950161 1201 82-83 0.6646871468810573 1201 84-85 0.5975519955050785 1201 86-87 0.40468750982523005 1201 88-89 0.7031491279085955 1201 90-91 0.9191626310786134 1201 92-93 0.7882310950953837 1202 1 -0.06937555580419286 1202 2 -0.043172768931242445 1202 3 9.705524621566042E-4 1202 4 -0.21101524968224084 1202 5 -0.10476486385665851 1202 6 -0.09982793650591759 1202 7 -0.09407675318983166 1202 8 0.028863831104821713 1202 9 0.018554392168042 1202 10-11 0.29346194544135784 1202 12-13 0.4019164718112407 1202 14-15 0.5769648404917334 1202 16-17 0.6197955330346403 1202 18-19 0.5805921198451713 1202 20-21 0.5864692895120882 1202 22-23 0.5599868885804185 1202 24-25 0.7577369653562442 1202 26-27 0.6986289563937902 1202 28-29 0.7412804583175756 1202 30-31 0.6009763567836686 1202 32-33 0.3486290512143029 1202 34-35 0.6935233789988686 1202 36-37 0.5262162016872907 1202 38-39 0.7618103298086822 1202 40-41 0.4225123681132814 1202 42-43 0.5627307482930277 1202 44-45 0.6629901426878178 1202 46-47 0.6546914078351165 1202 48-49 0.9496892720810237 1202 50-51 0.29891440654176193 1202 52-53 0.06037883719972825 1202 54-55 0.2986760309863996 1202 56-57 0.433060053730685 1202 58-59 0.3099264205993464 1202 60-61 0.4994537865397106 1202 62-63 0.24390710239583058 1202 64-65 0.15597954955429572 1202 66-67 0.20775335360763592 1202 68-69 0.19326306253888248 1202 70-71 0.5736255709323146 1202 72-73 0.416600777127087 1202 74-75 -0.07671589849593374 1202 76-77 0.17477345465898964 1202 78-79 0.02037467851270236 1202 80-81 0.26073550017341773 1202 82-83 0.6088051105017627 1202 84-85 0.2702460004561118 1202 86-87 -0.0908565650859785 1202 88-89 0.5722966900655209 1202 90-91 0.9492562700840992 1202 92-93 0.5795667963116173 1203 1 -0.28154660843577517 1203 2 -0.0056727689312410234 1203 3 0.1259705524621566 1203 4 0.06365580294934148 1203 5 -0.6162780217513983 1203 6 0.40280364244144806 1203 7 0.4559232468101655 1203 8 0.12853488373639976 1203 9 -0.42256402888458666 1203 10-11 -0.3540709492954832 1203 12-13 -0.29430063345191826 1203 14-15 0.03535299838646466 1203 16-17 -0.26967815117588856 1203 18-19 0.22203948826622621 1203 20-21 -0.8597478157510707 1203 22-23 0.17182899384357597 1203 24-25 0.010368544303617 1203 26-27 -0.23985788571147282 1203 28-29 -0.29079191010347927 1203 30-31 -0.13356311690053957 1203 32-33 0.41441852489850817 1203 34-35 -0.9194700420537671 1203 36-37 -0.13480353515481625 1203 38-39 -0.38013045966499703 1203 40-41 -0.7804481582025105 1203 42-43 -0.8234534622332816 1203 44-45 -0.34260196257534403 1203 46-47 -0.8709664869017217 1203 48-49 0.3473866405020729 1203 50-51 0.5387170381207085 1203 52-53 0.27304331088394207 1203 54-55 0.0912747151969242 1203 56-57 0.1256587379412082 1203 58-59 -0.6948433162427605 1203 60-61 -0.39906595030239345 1203 62-63 0.1266373655537265 1203 64-65 0.4684795495542957 1203 66-67 0.022720458870793436 1203 68-69 -0.14292114798742972 1203 70-71 -0.24298627117294558 1203 72-73 -0.14244527550449249 1203 74-75 -0.1096106353380435 1203 76-77 0.3721418757116197 1203 78-79 0.318894415354805 1203 80-81 -0.17183028930026722 1203 82-83 0.5324893210280806 1203 84-85 0.1888315267718994 1203 86-87 0.21144606649297515 1203 88-89 0.2556848479602607 1203 90-91 -0.60469109833695 1203 92-93 -0.3528345194778595 1204 1 0.26999559935643447 1204 2 0.22890515314668392 1204 3 0.22824327973488323 1204 4 0.05350969905323666 1204 5 0.36140054967717106 1204 6 0.373582863220669 1204 7 0.41046870135561875 1204 8 0.3180641045156207 1204 9 0.34651064644008756 1204 10-11 0.42088846628893606 1204 12-13 0.3248146262883367 1204 14-15 0.6406695568280227 1204 16-17 0.6881140566163211 1204 18-19 0.6335654622922036 1204 20-21 0.7463804310021729 1204 22-23 0.5630627600773437 1204 24-25 0.914589323524396 1204 26-27 0.7402557506521674 1204 28-29 0.5178525704160037 1204 30-31 0.4293427272553032 1204 32-33 0.3900678755478566 1204 34-35 0.5166095034007796 1204 36-37 0.2046851012088169 1204 38-39 0.3238062286466885 1204 40-41 0.4074576859533394 1204 42-43 0.2633971871173699 1204 44-45 0.7641350504116673 1204 46-47 0.5908841624489298 1204 48-49 0.8948704067358406 1204 50-51 0.7306813238349932 1204 52-53 0.4875319472475823 1204 54-55 0.6430198450670517 1204 56-57 0.7261051665126388 1204 58-59 0.6767070084325653 1204 60-61 0.6165590496976066 1204 62-63 0.520103274644633 1204 64-65 0.6267587703335167 1204 66-67 0.5468681861435201 1204 68-69 0.6387346961684202 1204 70-71 0.8931744431127697 1204 72-73 0.8666862180020019 1204 74-75 0.9683114425840316 1204 76-77 0.730908109477852 1204 78-79 0.5419057789911648 1204 80-81 0.611043087323111 1204 82-83 0.6526191911579531 1204 84-85 0.6476383449537195 1204 86-87 0.4529233392202485 1204 88-89 0.8512611466615638 1204 90-91 0.6777764341305854 1204 92-93 0.775209311690972 1316 1 0.5622033915642248 1316 2 0.556827231068759 1316 3 0.5009705524621566 1316 4 0.6574058029493415 1316 5 0.7899719782486017 1316 6 0.6528036424414481 1316 7 0.49539693102069293 1316 8 0.5972848837363998 1316 9 0.4606596553259408 1316 10-11 0.4197777349150442 1316 12-13 0.5650743665480817 1316 14-15 0.5690701036496222 1316 16-17 -0.04599394064957352 1316 18-19 -0.03782893278640387 1316 20-21 0.683837710564724 1316 22-23 0.8099868885804185 1316 24-25 0.23405275482993204 1316 26-27 0.6433657984990475 1316 28-29 1.280754142528103 1316 30-31 0.545713198888933 1316 32-33 0.2828395775300834 1316 34-35 0.41194443163044525 1316 36-37 0.38411093852939615 1316 38-39 0.030231382440263133 1316 40-41 0.8461965786395993 1316 42-43 0.5548360114509236 1316 44-45 -0.5975361731016591 1316 46-47 0.15205982888775793 1316 48-49 -0.5424159910768722 1316 50-51 -0.3116119092477163 1316 52-53 0.7130104161470996 1316 54-55 0.3881497151969242 1316 56-57 0.8962179484675232 1316 58-59 0.3915053679677669 1316 60-61 1.0705064181186614 1316 62-63 0.5649597339747814 1316 64-65 -0.13349413465623172 1316 66-67 0.31301651150236864 1316 68-69 0.4064209572757278 1316 70-71 -0.28163758696242525 1316 72-73 -0.9097150123466022 1316 74-75 -0.6030316879696187 1316 76-77 0.6352997704484622 1316 78-79 0.7309009943021749 1316 80-81 1.1949460264892124 1316 82-83 0.545647215764923 1316 84-85 1.0334038951929543 1316 86-87 0.24598554017718754 1316 88-89 0.9801914269076306 1316 90-91 0.6650457437683102 1316 92-93 1.7006194278905653 1206 1 0.5622033915642248 1206 2 0.12825580249732838 1206 3 0.072399123890726 1206 4 0.6574058029493415 1206 5 0.21854340682003226 1206 6 0.08137507101287866 1206 7 -0.008362467475549806 1206 8 0.025856312307830365 1206 9 0.6711859711154133 1206 10-11 0.6303040507045168 1206 12-13 0.42221722369094294 1206 14-15 0.33222799838646466 1206 16-17 0.5874647059669726 1206 18-19 0.8738252025519344 1206 20-21 1.0710557556774987 1206 22-23 1.1182575652721454 1206 24-25 0.6889399728750476 1206 26-27 0.746749257145666 1206 28-29 -0.12338119581777107 1206 30-31 0.5494725973851686 1206 32-33 0.09298995346993877 1206 34-35 0.5604406722319482 1206 36-37 0.8897500362737532 1206 38-39 0.9569231117635724 1206 40-41 0.33115898465463545 1206 42-43 1.0604751091952878 1206 44-45 0.6975766088532254 1206 46-47 0.19153351309827826 1206 48-49 1.5929223547877882 1206 50-51 1.2128241809778473 1206 52-53 0.7261683108839421 1206 54-55 0.281006858054063 1206 56-57 1.1725337379412082 1206 58-59 0.9591745409001007 1206 60-61 0.24155904969760655 1206 62-63 1.3431552226965877 1206 64-65 1.120265263840011 1206 66-67 0.6945954588707934 1206 68-69 -0.11613543370171442 1206 70-71 0.2503173002556238 1206 72-73 0.24817972449550751 1206 74-75 0.9439607932333871 1206 76-77 0.6578561614259044 1206 78-79 0.4996979867833744 1206 80-81 -0.408437432157406 1206 82-83 0.85391789245665 1206 84-85 1.13302795534333 1206 86-87 0.35876749506440575 1206 88-89 0.7132741336745454 1206 90-91 0.3060231873773418 1206 92-93 -0.2523880909064289 1207 1 0.0903635918145369 1207 2 0.06183348889103968 1207 3 0.08482537098531395 1207 4 0.08231193561517358 1207 5 0.19297573294196724 1207 6 0.018886245695512116 1207 7 0.15022862853732732 1207 8 0.043467612146912415 1207 9 0.12300073957598556 1207 10-11 0.05865198562316465 1207 12-13 -0.030044532075201857 1207 14-15 -0.15963683265233186 1207 16-17 -0.05221882702069536 1207 18-19 -0.06386476705292665 1207 20-21 -0.057318998479480854 1207 22-23 -0.13058026773339293 1207 24-25 0.09735227396569712 1207 26-27 -0.11262853652499416 1207 28-29 -0.10237279245642128 1207 30-31 -0.30749146483545786 1207 32-33 -0.1988480583305261 1207 34-35 -0.0624808367971994 1207 36-37 -0.15915037495456374 1207 38-39 -0.3532413795648708 1207 40-41 -0.3007469692162772 1207 42-43 -0.2296643508440397 1207 44-45 -0.23899589248773623 1207 46-47 -0.22523745060635747 1207 48-49 -0.035005412063632946 1207 50-51 -0.24261118465776121 1207 52-53 -0.3201395739721278 1207 54-55 -0.13062375163661955 1207 56-57 -0.26864273264702376 1207 58-59 -0.28032125741923153 1207 60-61 -0.22026823440751997 1207 62-63 -0.2636747433323805 1207 64-65 -0.15503421765471614 1207 66-67 -0.04664358994021711 1207 68-69 -0.051009383281545695 1207 70-71 -0.21848298581624448 1207 72-73 0.06795444289350172 1207 74-75 -0.17265819478735978 1207 76-77 -0.44419104043356583 1207 78-79 -0.2788645020419409 1207 80-81 -0.13416602146547518 1207 82-83 -0.16557075406578292 1207 84-85 -0.34153846697027745 1207 86-87 -0.5694910423931319 1207 88-89 -0.1575465350184615 1207 90-91 -0.0025321496510954944 1207 92-93 -0.14247391246910013 1210 1 -0.12121369386291292 1210 2 -0.25724312069003474 1210 3 -0.33822542743734374 1210 4 -0.0059107799652338144 1210 5 0.02112775714307702 1210 6 -0.392422488211821 1210 7 -0.12824760746119068 1210 8 -0.34241360872591287 1210 9 -0.3489145313971491 1210 10-11 -0.2817562508030207 1210 12-13 -0.21382010581373123 1210 14-15 -0.19792275538237902 1210 16-17 -0.042291216502519546 1210 18-19 -0.2964906624875425 1210 20-21 -0.4727346247962956 1210 22-23 -0.34513080515139904 1210 24-25 -0.2949078376059333 1210 26-27 -0.048117935962729064 1210 28-29 -0.21078248799292965 1210 30-31 -0.4411950264482769 1210 32-33 -0.42226489218691654 1210 34-35 -0.3837445395412047 1210 36-37 -0.6804222537477784 1210 38-39 -0.4549577209715281 1210 40-41 -0.18104489187084738 1210 42-43 -0.2735162763036314 1210 44-45 -0.2067665354396695 1210 46-47 -0.1338433713238345 1210 48-49 -0.030911098191396036 1210 50-51 -0.04435456991949138 1210 52-53 -0.39066585997033343 1210 54-55 -0.6796894807829759 1210 56-57 -0.6302300811542665 1210 58-59 -0.19445072830306032 1210 60-61 0.04432286879308123 1210 62-63 -0.0541886394713984 1210 64-65 0.08311522794625859 1210 66-67 -0.18103268183271837 1210 68-69 -0.023888484670848698 1210 70-71 0.029391241389866707 1210 72-73 -0.015639370981880063 1210 74-75 -0.2572237006646816 1210 76-77 -0.2861495815748114 1210 78-79 -0.2513788258512264 1210 80-81 -0.5331682289987612 1210 82-83 -0.31047550309251903 1210 84-85 -0.42494045815272585 1210 86-87 -0.2858843355170748 1210 88-89 -0.0649024635975266 1210 90-91 -0.10908808326157526 1210 92-93 0.11929173680354666 1211 1 0.5622033915642248 1211 2 0.556827231068759 1211 3 0.5009705524621566 1211 4 0.6574058029493415 1211 5 0.7899719782486017 1211 6 0.6528036424414481 1211 7 0.7059232468101655 1211 8 0.5972848837363998 1211 9 0.6711859711154133 1211 10-11 0.6303040507045168 1211 12-13 0.5650743665480817 1211 14-15 1.3322279983864647 1211 16-17 1.4803218488241114 1211 18-19 1.4095394882662262 1211 20-21 1.4996271842489293 1211 22-23 0.04682899384357597 1211 24-25 0.260368544303617 1211 26-27 0.3538921142885272 1211 28-29 0.41233308989652073 1211 30-31 1.8351868830994604 1211 32-33 1.9144185248985082 1211 34-35 2.096154957946233 1211 36-37 2.0683214648451838 1211 38-39 2.135494540335003 1211 40-41 2.1883018417974895 1211 42-43 2.2390465377667184 1211 44-45 2.376148037424656 1211 46-47 2.4415335130982783 1211 48-49 -0.12136335949792709 1211 50-51 0.8199670381207085 1211 52-53 1.976168310883942 1211 54-55 1.3881497151969242 1211 56-57 -0.07746626205879181 1211 58-59 0.10203168375723948 1211 60-61 -0.008440950302393446 1211 62-63 -0.01398763444627349 1211 64-65 0.15597954955429572 1211 66-67 0.44459545887079344 1211 68-69 0.6695788520125703 1211 70-71 0.9288887288270544 1211 72-73 1.2481797244955075 1211 74-75 1.2653893646619565 1211 76-77 1.3721418757116197 1211 78-79 1.678269415354805 1211 80-81 1.9844197106997328 1211 82-83 0.28248932102808055 1211 84-85 0.4544565267718994 1211 86-87 2.430196066492975 1211 88-89 -1.0724401520397393 1211 90-91 -0.72969109833695 1211 92-93 0.06904048052214051 1212 1 -0.18779660843577517 1212 2 0.306827231068759 1212 3 0.5009705524621566 1212 4 0.15740580294934148 1212 5 0.20663864491526596 1212 6 0.31947030910811236 1212 7 -0.0857434198564988 1212 8 0.43061821706973546 1212 9 0.25451930444874904 1212 10-11 -0.2863626159621475 1212 12-13 0.19007436654808174 1212 14-15 -0.04277200161353534 1212 16-17 -0.29051148450922426 1212 18-19 -0.5279605117337738 1212 20-21 -0.29203948241774214 1212 22-23 0.3801623271769117 1212 24-25 0.010368544303617 1212 26-27 -0.14610788571147282 1212 28-29 0.28733308989652073 1212 30-31 -0.060646450233868165 1212 32-33 0.41441852489850817 1212 34-35 0.033654957946232855 1212 36-37 0.17248813151185516 1212 38-39 0.5938278736683316 1212 40-41 0.5216351751308181 1212 42-43 -0.09428679556661024 1212 44-45 0.08448137075798456 1212 46-47 0.8790335130982783 1212 48-49 -0.1421966928312628 1212 50-51 0.11163370478737988 1212 52-53 0.10116831088394207 1212 54-55 0.8881497151969242 1212 56-57 0.6100337379412082 1212 58-59 1.5811983504239109 1212 60-61 0.8873923830309352 1212 62-63 0.6318456988870551 1212 64-65 0.6976462162209671 1212 66-67 0.6945954588707934 1212 68-69 1.0445788520125703 1212 70-71 0.574722062160383 1212 72-73 0.6023463911621718 1212 74-75 1.036222697995285 1212 76-77 0.7054752090449483 1212 78-79 0.7616027486881336 1212 80-81 0.9844197106997328 1212 82-83 0.44915598769475196 1212 84-85 0.7044565267718994 1212 86-87 1.2218627331596466 1212 88-89 1.1775598479602607 1212 90-91 1.7494755683297214 1212 92-93 0.8607071471888119 1213 1 -0.643936447352548 1213 2 -0.6711405522859728 1213 3 -0.8427903906918068 1213 4 -0.8385671163923192 1213 5 -0.7133547405561131 1213 6 -0.7811872528544583 1213 7 -0.9042086546722672 1213 8 -0.9145629042874148 1213 9 -0.9178183477826849 1213 10-11 -0.9290516163901472 1213 12-13 -1.0046700013753451 1213 14-15 -1.617112494201372 1213 16-17 -1.4833760617630318 1213 18-19 -1.5920946893922334 1213 20-21 -1.7323676797641454 1213 22-23 -1.7432959040203144 1213 24-25 -1.7088155341369102 1213 26-27 -1.6787914388806087 1213 28-29 -1.5326885045939633 1213 30-31 -1.85256845715967 1213 32-33 -2.040466499030522 1213 34-35 -1.8257546953746484 1213 36-37 -1.9001038882539234 1213 38-39 -2.069302938362327 1213 40-41 -2.0034222156321846 1213 42-43 -1.8943140318609224 1213 44-45 -1.9145604953172608 1213 46-47 -2.0206469468060106 1213 48-49 -1.8228341193905422 1213 50-51 -1.9284279659647368 1213 52-53 -2.110968376404493 1213 54-55 -2.082901994853266 1213 56-57 -1.9043017937501645 1213 58-59 -1.9168197228028205 1213 60-61 -1.8463655446761535 1213 62-63 -1.9163455193534773 1213 64-65 -1.878921816151319 1213 66-67 -1.7527898568756797 1213 68-69 -1.8324638700605007 1213 70-71 -1.819798092697404 1213 72-73 -1.8124599393308003 1213 74-75 -1.889215514525624 1213 76-77 -2.401933063006723 1213 78-79 -2.2776661513546657 1213 80-81 -2.120692930831723 1213 82-83 -2.0167986444207315 1213 84-85 -2.150189206857142 1213 86-87 -2.234330605620947 1213 88-89 -2.081486492649052 1213 90-91 -2.0074429367868163 1213 92-93 -2.1190650406638056 1214 1 -0.6199093757721315 1214 2 -0.7795305135845148 1214 3 -0.78872484611205 1214 4 -0.9790167505179284 1214 5 -1.0253229018550911 1214 6 -1.0568528708443594 1214 7 -1.0030851783356525 1214 8 -0.9607190047924377 1214 9 -0.9665327586318355 1214 10-11 -0.8350232338061758 1214 12-13 -1.1410176619678012 1214 14-15 -1.8521530774398443 1214 16-17 -1.7542860578511963 1214 18-19 -1.7434093127707158 1214 20-21 -1.7706255701256666 1214 22-23 -2.077279884963943 1214 24-25 -1.7376871912764216 1214 26-27 -1.770864852658974 1214 28-29 -1.7496889451002389 1214 30-31 -1.7578137649886756 1214 32-33 -1.8020429138571643 1214 34-35 -1.7622377834665954 1214 36-37 -1.9426960335346024 1214 38-39 -2.087123735750545 1214 40-41 -1.9584901219095698 1214 42-43 -1.9884323993687332 1214 44-45 -1.8908642762499994 1214 46-47 -2.1715578673294544 1214 48-49 -1.9042538326022722 1214 50-51 -1.794096474517005 1214 52-53 -1.8264888504900014 1214 54-55 -1.6056934474732003 1214 56-57 -1.5739017772888673 1214 58-59 -1.5117077848104898 1214 60-61 -1.5463541064916342 1214 62-63 -1.752484069961504 1214 64-65 -1.733197378507917 1214 66-67 -1.6218335754778792 1214 68-69 -1.5216071492836036 1214 70-71 -1.4353368058456581 1214 72-73 -1.3470892320825882 1214 74-75 -1.6892444655389482 1214 76-77 -1.9246824924024395 1214 78-79 -2.1901686922278287 1214 80-81 -2.199961365126576 1214 82-83 -2.0969662849343322 1214 84-85 -2.112944639786754 1214 86-87 -2.145306201815515 1214 88-89 -1.8770415778336442 1214 90-91 -1.8132944683952772 1214 92-93 -2.0259044319859605 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 0.0 4 1.0 5 0.0 6 2.0 7 17.0 8 42.0 9 59.0 10 72.0 11 162.0 12 299.0 13 424.0 14 482.0 15 491.0 16 473.0 17 569.0 18 562.0 19 648.0 20 682.0 21 755.0 22 860.0 23 949.0 24 1026.0 25 1219.0 26 1462.0 27 1772.0 28 2001.0 29 2261.0 30 2948.0 31 3627.0 32 4775.0 33 6382.0 34 9392.0 35 14542.0 36 25928.0 37 53583.0 38 76031.0 39 3363.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.098839557856856 22.904043186074695 10.971411259226617 25.025705996841836 2 15.8841053370851 21.77293863293138 38.12754472052256 24.215411309460965 3 15.56172683665741 25.97048495558973 31.617360170755777 26.850428036997087 4 11.802858795339999 16.376564120925025 41.66276497103565 30.15781211269933 5 12.416455510264038 37.81260328302303 37.48301568065807 12.287925526054865 6 29.53961259524465 40.9767740750941 17.865142752226202 11.61847057743505 7 26.950211373306832 32.51476859098232 25.247290703705573 15.28772933200527 8 23.935904085632583 36.93685423274473 22.301834656042153 16.82540702558053 9 23.986394994973857 14.969177594888484 24.49222210492011 36.55220530521754 10-11 23.019317407239953 26.45408415017867 31.133454664796968 19.39314377778441 12-13 22.377800524187442 24.95788599152671 31.000729823144113 21.663583661141736 14-15 20.547322713387693 26.300086523256855 26.62047502874546 26.53211573460999 16-17 20.639860460846414 30.711925089507027 27.611310015606357 21.03690443404021 18-19 20.630403400341045 29.49525040105939 29.492496345138285 20.381849853461276 20-21 21.037133939227026 29.35738547691178 28.843064353254384 20.76241623060681 22-23 21.118669586012842 28.824412343876947 29.084674809622825 20.97224326048738 24-25 20.530298335846435 28.586215364560026 29.626340032635863 21.25714626695768 26-27 20.879751396558294 29.50306852660231 29.003887871401883 20.61329220543751 28-29 21.069868745079948 29.16135932267963 28.87814797219296 20.89062396004746 30-31 20.784600837250295 28.835790613983548 29.179136677438304 21.20047187132785 32-33 20.941259633612873 29.189101107607286 29.149625671885687 20.720013586894158 34-35 20.820251537684754 29.01358670705958 29.229780593041404 20.936381162214264 36-37 20.750854338940183 29.059458318128513 29.15608066703847 21.033606675892838 38-39 20.834165295363736 29.082179488062206 29.267850462570888 20.81580475400317 40-41 20.71083325300541 29.16798633966317 29.37316680207659 20.748013605254823 42-43 20.759393733532853 29.13755749157708 29.316802687989423 20.786246086900643 44-45 20.587675027598188 28.81480410449902 29.44021004459316 21.157310823309626 46-47 20.47030310819588 28.91542484950163 29.803152949492677 20.811119092809815 48-49 20.72546589552924 29.217387312953274 29.336270999724594 20.720875791792896 50-51 20.63458184154962 28.964472597080693 29.418204351418343 20.982741209951346 52-53 20.668501173916336 28.74867174177972 29.800950608302102 20.781876476001845 54-55 20.522583310382814 29.270403011108055 29.446892499770495 20.76012117873864 56-57 20.678559707332973 29.111556354239106 29.282768402426345 20.92711553600158 58-59 20.689718485061302 28.982543595110556 29.453494723603097 20.87424319622505 60-61 20.469020029055557 28.96191794215052 29.571487915321182 20.997574113472737 62-63 20.593783995923932 29.114510894761242 29.294445438980617 20.99725967033421 64-65 20.583915136993532 28.917133716153252 29.417920929784213 21.081030217068992 66-67 20.44781668709291 28.83841235305728 29.542543962323908 21.1712269975259 68-69 20.5279151002722 29.223297851342856 29.38969140307633 20.85909564530862 70-71 20.742734913268794 28.894871406473055 29.33185529957725 21.030538380680905 72-73 20.742306465346104 28.937650755181615 29.18803919003587 21.132003589436408 74-75 20.61053810527089 29.15834698907999 29.239592943995373 20.991521961653746 76-77 20.59234608985025 29.178725113316883 29.15715187331459 21.071776923518275 78-79 20.87578174307189 28.91387916690573 29.136726146078374 21.073612943944 80-81 20.80472063602813 29.09589996878615 29.090391642032206 21.008987753153516 82-83 20.81525019565362 29.207815128557623 29.173848282953003 20.80308639283575 84-85 20.924735032613135 29.20241259174596 29.039691173567984 20.83316120207292 86-87 20.685816843158587 28.998379671068637 29.19300274951 21.12280073626278 88-89 20.77624313196271 28.93113372533359 29.245560161023054 21.047062981680646 90-91 21.144145012554105 29.02247804752659 29.02729772281818 20.806079217101125 92-93 21.291357594007053 28.856905478848415 29.018709606345478 20.83302732079906 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2.0 1 2.0 2 1.0 3 1.5 4 2.0 5 1.0 6 1.0 7 2.0 8 2.0 9 2.0 10 4.0 11 3.5 12 3.5 13 13.5 14 22.0 15 27.5 16 47.0 17 97.5 18 161.0 19 263.0 20 438.5 21 654.5 22 1011.5 23 1504.0 24 2024.0 25 2630.0 26 3347.0 27 4124.5 28 4957.5 29 5393.0 30 5805.0 31 6541.0 32 7244.0 33 7793.0 34 8300.5 35 8711.0 36 8900.5 37 9011.5 38 8984.5 39 8897.5 40 8690.5 41 8448.0 42 8264.0 43 8181.0 44 8032.5 45 7556.0 46 6999.5 47 6512.0 48 5976.0 49 5536.0 50 5062.5 51 4655.5 52 4283.5 53 3833.0 54 3473.0 55 3161.0 56 2882.0 57 2730.0 58 2675.0 59 2513.5 60 2277.0 61 2000.0 62 1782.5 63 1594.5 64 1416.5 65 1273.5 66 1123.0 67 981.0 68 868.0 69 750.5 70 628.5 71 585.0 72 499.5 73 403.0 74 365.0 75 318.0 76 300.5 77 267.5 78 195.0 79 159.0 80 141.0 81 117.0 82 105.5 83 82.0 84 55.0 85 32.5 86 20.0 87 16.5 88 12.5 89 10.0 90 7.5 91 5.5 92 2.0 93 1.0 94 2.0 95 1.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.007802997268950955 2 0.005507998072200675 3 0.0045899983935005625 4 0.005048998232850619 5 0.007802997268950955 6 0.0022949991967502813 7 0.001835999357400225 8 0.001835999357400225 9 0.001835999357400225 10-11 0.002065499277075253 12-13 0.001835999357400225 14-15 0.002065499277075253 16-17 0.0022949991967502813 18-19 0.002065499277075253 20-21 0.0022949991967502813 22-23 0.00367199871480045 24-25 0.0029834989557753653 26-27 0.00367199871480045 28-29 0.0029834989557753653 30-31 0.004130998554150506 32-33 0.00367199871480045 34-35 0.0022949991967502813 36-37 0.0029834989557753653 38-39 0.0029834989557753653 40-41 0.00367199871480045 42-43 0.0032129988754503935 44-45 0.0029834989557753653 46-47 0.0025244991164253094 48-49 0.0022949991967502813 50-51 0.0022949991967502813 52-53 0.002065499277075253 54-55 0.0022949991967502813 56-57 0.0029834989557753653 58-59 0.00367199871480045 60-61 0.0029834989557753653 62-63 0.00367199871480045 64-65 0.00367199871480045 66-67 0.00367199871480045 68-69 0.00367199871480045 70-71 0.00367199871480045 72-73 0.0016064994377251967 74-75 0.00367199871480045 76-77 0.0011474995983751406 78-79 0.0011474995983751406 80-81 0.005966997911550731 82-83 0.0025244991164253094 84-85 0.00367199871480045 86-87 0.00367199871480045 88-89 0.00367199871480045 90-91 0.00367199871480045 92-93 0.004130998554150506 >>END_MODULE >>Sequence Length Distribution pass #Length Count 93 217865.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.64224925046146 #Duplication Level Percentage of deduplicated Percentage of total 1 97.04906982382249 92.81991325615468 2 2.323318008347071 4.444147200848326 3 0.3731124001100515 1.0705592750929034 4 0.12363042613648646 0.4729716812594641 5 0.06155796781143917 0.2943771250386774 6 0.03561796547771322 0.20439493992082677 7 0.008289672134245687 0.05549900219276917 8 0.009231038660508778 0.07063018403272212 9 0.005011795572318566 0.043140546118804635 >10 0.008727748832501016 0.10976679401257211 >50 5.094745426818624E-4 0.0370327413104371 >100 0.0019236785525287283 0.37756725401781877 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAAC 298 0.13678195212631675 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10-11 0.0 0.0 0.0 12-13 0.0 0.0 0.0 14-15 0.0 0.0 2.2949991967502811E-4 16-17 0.0 0.0 4.5899983935005623E-4 18-19 0.0 0.0 4.5899983935005623E-4 20-21 0.0 0.0 4.5899983935005623E-4 22-23 0.0 0.0 4.5899983935005623E-4 24-25 0.0 0.0 0.001835999357400225 26-27 0.0 0.0 0.0043604984738255335 28-29 0.0 0.0 0.01606499437725197 30-31 0.0 0.0 0.027310490441328345 32-33 0.0 0.0 0.03970348610377987 34-35 0.0 0.0 0.06632547678608312 36-37 0.0 0.0 0.1337984531705414 38-39 0.0 0.0 0.25107291212448074 40-41 0.0 0.0 0.3933628623229982 42-43 0.0 0.0 0.5420788102724163 44-45 0.0 0.0 0.7160397493860877 46-47 0.0 0.0 0.9175406788607624 48-49 0.0 0.0 1.1130746104238862 50-51 0.0 0.0 1.3265095357216625 52-53 0.0 0.0 1.5140109700961606 54-55 0.0 0.0 1.7129874004544097 56-57 0.0 0.0 1.9401923209326877 58-59 0.0 0.0 2.179790237073417 60-61 0.0 0.0 2.418011153696096 62-63 0.0 0.0 2.639249076262823 64-65 0.0 0.0 2.8595689991508504 66-67 0.0 0.0 3.101232414568655 68-69 0.0 0.0 3.3591903242833867 70-71 0.0 0.0 3.6017717393798914 72-73 0.0 0.0 3.8317306588942692 74-75 0.0 0.0 4.077754572785899 76-77 0.0 0.0 4.308172492139628 78-79 0.0 0.0 4.566130401854359 80-81 0.0 0.0 4.805498818075414 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGATCG 20 0.0029364743 65.25 9 CTTGGCG 65 2.2215834E-4 33.46154 9 GTATAGG 65 2.2215834E-4 33.46154 1 TTACACC 65 2.2215834E-4 33.46154 4 GGCAGTA 55 0.003962515 31.636362 7 GTCTAGT 55 0.003962515 31.636362 1 TATCCTG 70 3.4317037E-4 31.071426 5 ACTGTGC 85 2.967343E-5 30.705881 8 ACAGTGC 75 5.1407807E-4 29.0 8 TAGTACC 60 0.006064175 29.0 4 CAGAAGT 120 1.0604899E-5 25.375 4 TAATACC 105 1.2513236E-4 24.857143 4 GTATAAG 90 0.0014905318 24.166668 1 GTCCAGA 95 0.0020416032 22.894737 1 TAGGACT 215 4.5456545E-9 22.255814 4 TGGAGTG 140 3.4948076E-5 21.75 5 TAATACT 140 3.4948076E-5 21.75 4 ACCATGC 120 3.0922738E-4 21.75 8 TTATACT 100 0.0027502622 21.75 4 CTAATAC 100 0.0027502622 21.75 3 >>END_MODULE