FastQCFastQC Report
Wed 5 Nov 2014
DP2A_AGGCAGAA-TATCCTCT_L007_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDP2A_AGGCAGAA-TATCCTCT_L007_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217865
Sequences flagged as poor quality0
Sequence length93
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAAC2980.13678195212631675No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATCG200.002936474365.259
CTTGGCG652.2215834E-433.461549
GTATAGG652.2215834E-433.461541
TTACACC652.2215834E-433.461544
GGCAGTA550.00396251531.6363627
GTCTAGT550.00396251531.6363621
TATCCTG703.4317037E-431.0714265
ACTGTGC852.967343E-530.7058818
ACAGTGC755.1407807E-429.08
TAGTACC600.00606417529.04
CAGAAGT1201.0604899E-525.3754
TAATACC1051.2513236E-424.8571434
GTATAAG900.001490531824.1666681
GTCCAGA950.002041603222.8947371
TAGGACT2154.5456545E-922.2558144
TGGAGTG1403.4948076E-521.755
TAATACT1403.4948076E-521.754
ACCATGC1203.0922738E-421.758
TTATACT1000.002750262221.754
CTAATAC1000.002750262221.753
Produced by FastQC (version 0.11.2)