##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename DP2A_AGGCAGAA-TATCCTCT_L007_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 217865 Sequences flagged as poor quality 0 Sequence length 93 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.791788492874026 33.0 33.0 33.0 33.0 33.0 2 32.80781676726413 33.0 33.0 33.0 33.0 33.0 3 32.81225070571225 33.0 33.0 33.0 33.0 33.0 4 36.766974961558766 37.0 37.0 37.0 37.0 37.0 5 36.62581874096344 37.0 37.0 37.0 37.0 37.0 6 36.62062745278039 37.0 37.0 37.0 37.0 37.0 7 36.603910678631266 37.0 37.0 37.0 37.0 37.0 8 36.61314575539899 37.0 37.0 37.0 37.0 37.0 9 36.55478851582402 37.0 37.0 37.0 37.0 37.0 10-11 36.584820875312694 37.0 37.0 37.0 37.0 37.0 12-13 36.619351433226996 37.0 37.0 37.0 37.0 37.0 14-15 38.84643930874624 40.0 37.0 40.0 37.0 40.0 16-17 38.78264062607578 40.0 37.0 40.0 37.0 40.0 18-19 38.76583893695637 40.0 37.0 40.0 37.0 40.0 20-21 38.7126385605765 40.0 37.0 40.0 37.0 40.0 22-23 38.66898308585592 40.0 37.0 40.0 37.0 40.0 24-25 38.633314208340025 40.0 37.0 40.0 37.0 40.0 26-27 38.54620980882657 40.0 37.0 40.0 37.0 40.0 28-29 38.45656943520069 40.0 37.0 40.0 37.0 40.0 30-31 38.42474927133776 40.0 37.0 40.0 37.0 40.0 32-33 38.3396185711335 40.0 37.0 40.0 37.0 40.0 34-35 38.27448419893053 40.0 37.0 40.0 37.0 40.0 36-37 38.20167993941202 40.0 37.0 40.0 37.0 40.0 38-39 38.12584169095541 40.0 37.0 40.0 37.0 40.0 40-41 37.97475959883414 40.0 37.0 40.0 33.0 40.0 42-43 37.883280930851676 40.0 37.0 40.0 33.0 40.0 44-45 37.77264131457554 40.0 37.0 40.0 33.0 40.0 46-47 37.69874463543938 40.0 37.0 40.0 33.0 40.0 48-49 37.534551212907076 40.0 37.0 40.0 33.0 40.0 50-51 37.34812154315746 40.0 37.0 40.0 33.0 40.0 52-53 37.31321690037409 40.0 37.0 40.0 33.0 40.0 54-55 37.154311155991095 38.5 37.0 40.0 33.0 40.0 56-57 37.016925619076034 37.0 37.0 40.0 33.0 40.0 58-59 36.83642852225002 37.0 37.0 40.0 33.0 40.0 60-61 36.62454731140844 37.0 37.0 40.0 33.0 40.0 62-63 36.43638491726528 37.0 37.0 40.0 33.0 40.0 64-65 36.132288343699074 37.0 37.0 40.0 33.0 40.0 66-67 35.91641842425355 37.0 37.0 40.0 33.0 40.0 68-69 35.613432630298576 37.0 33.0 40.0 33.0 40.0 70-71 35.48372157069745 37.0 33.0 38.5 33.0 40.0 72-73 35.173063594427745 37.0 33.0 37.0 27.0 40.0 74-75 34.8199825580061 37.0 33.0 37.0 27.0 40.0 76-77 33.3137699951805 35.0 33.0 37.0 27.0 37.0 78-79 34.49116425309251 37.0 33.0 37.0 27.0 37.0 80-81 34.624434856447806 37.0 33.0 37.0 27.0 37.0 82-83 34.482252771211535 37.0 33.0 37.0 27.0 37.0 84-85 34.35184862185298 37.0 33.0 37.0 27.0 37.0 86-87 34.21665710417001 37.0 33.0 37.0 27.0 37.0 88-89 34.025261056158634 37.0 33.0 37.0 27.0 37.0 90-91 33.85439836596058 37.0 33.0 37.0 27.0 37.0 92-93 32.77421797902371 35.0 33.0 37.0 24.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1201 1 0.0729450681414292 1201 2 0.061944343305633254 1201 3 0.07063518652190481 1201 4 0.06084778901371379 1201 5 0.0812134032178804 1201 6 0.09865411450579131 1201 7 0.14490564955538332 1201 8 0.13114949695772538 1201 9 0.026058732381542882 1201 10-11 0.09093365305412959 1201 12-13 0.1627397212772692 1201 14-15 0.2701476212348197 1201 16-17 0.38418078378909826 1201 18-19 0.34894885103978623 1201 20-21 0.3285646155314623 1201 22-23 0.4655899557763803 1201 24-25 0.5363583868614796 1201 26-27 0.3764573647701397 1201 28-29 0.5489418690631851 1201 30-31 0.40495242790225205 1201 32-33 0.3163565324143889 1201 34-35 0.29620785703561836 1201 36-37 0.27669368793706184 1201 38-39 0.604504844143193 1201 40-41 0.38836150178747886 1201 42-43 0.4082360684501083 1201 44-45 0.5459651536879946 1201 46-47 0.36232657960157155 1201 48-49 0.6089005327827905 1201 50-51 0.589158797536065 1201 52-53 0.32556313451320307 1201 54-55 0.6603391546194857 1201 56-57 0.7035480323950623 1201 58-59 0.684529662638667 1201 60-61 0.7635224670796816 1201 62-63 0.7859376981814847 1201 64-65 0.6724628008642384 1201 66-67 0.8803694351652211 1201 68-69 0.8156788381437039 1201 70-71 0.7986686866161605 1201 72-73 1.0792735899146493 1201 74-75 0.8725662141678612 1201 76-77 0.7352520505941982 1201 78-79 0.8600393355100024 1201 80-81 0.6664771433538377 1201 82-83 0.6716276813377817 1201 84-85 0.593903324898811 1201 86-87 0.6411681106834024 1201 88-89 0.6165702373020139 1201 90-91 0.5930332988662315 1201 92-93 1.0841374525172682 1202 1 0.04091407040167638 1202 2 0.05191313030380229 1202 3 0.017723766868471102 1202 4 -0.022953739347059354 1202 5 0.06481043260115626 1202 6 -0.18013180735407275 1202 7 -0.16917249058023032 1202 8 0.08484662320893221 1202 9 -0.16354410907793238 1202 10-11 -0.12967230970553345 1202 12-13 0.02755513404230925 1202 14-15 0.14513954890676217 1202 16-17 0.28847526231670173 1202 18-19 0.18256740663122173 1202 20-21 0.35015002076233515 1202 22-23 0.6465715508684013 1202 24-25 0.4731951105725969 1202 26-27 0.2451690212118578 1202 28-29 0.5872639011372343 1202 30-31 0.47871736170916535 1202 32-33 0.41091040175030713 1202 34-35 0.42439642661909005 1202 36-37 0.5041934188594652 1202 38-39 0.528302067262338 1202 40-41 0.17144728372699802 1202 42-43 0.06937588117209259 1202 44-45 0.4672222842444427 1202 46-47 0.3422372458377083 1202 48-49 0.488811199018933 1202 50-51 0.6775023558182696 1202 52-53 0.5364123434250558 1202 54-55 0.49306437257234137 1202 56-57 0.7482956376044072 1202 58-59 0.7274098692902697 1202 60-61 0.9130650351563148 1202 62-63 0.8433373322359472 1202 64-65 0.9190831861833573 1202 66-67 0.7773665291271143 1202 68-69 0.5858973291565164 1202 70-71 0.7477269150092241 1202 72-73 0.8385729118390941 1202 74-75 0.9171416097119334 1202 76-77 0.9973928319955547 1202 78-79 0.7503417787346294 1202 80-81 0.4411623225595207 1202 82-83 0.5724941112161588 1202 84-85 0.6720004080976096 1202 86-87 0.719168325945482 1202 88-89 0.6656123210389708 1202 90-91 0.5380553632295886 1202 92-93 0.7420214262552918 1203 1 0.16722985987536276 1203 2 0.1466499724090653 1203 3 0.14403955634215748 1203 4 0.15599362907398984 1203 5 0.2542841168116823 1203 6 0.23039450843540266 1203 7 -0.07575143794865369 1203 8 -0.5276533767910649 1203 9 0.1535611540799593 1203 10-11 0.19466979555762265 1203 12-13 0.16571302877915173 1203 14-15 0.3114224436436004 1203 16-17 0.42104105179038953 1203 18-19 0.21924503821016827 1203 20-21 0.37991975760444063 1203 22-23 0.2140057613947164 1203 24-25 0.22286616320417352 1203 26-27 0.29599138963290983 1203 28-29 -0.21438083570487265 1203 30-31 -0.1928286909224184 1203 32-33 -0.40816854561811766 1203 34-35 -0.41030752074933474 1203 36-37 0.1536999978068323 1203 38-39 0.6656375935781327 1203 40-41 0.13740123109541713 1203 42-43 -0.06746622409106351 1203 44-45 -0.1268566631239736 1203 46-47 -0.6919732804780807 1203 48-49 -0.8294453799284369 1203 50-51 -0.3667410652343577 1203 52-53 -0.3007587092065265 1203 54-55 -0.23275799584870782 1203 56-57 -0.4572964676587503 1203 58-59 0.4639230271850039 1203 60-61 0.04102556147210379 1203 62-63 0.5622518059201553 1203 64-65 0.402142396709678 1203 66-67 -0.27855452350446086 1203 68-69 0.9299762765249326 1203 70-71 0.2788124413250159 1203 72-73 0.1482768592075132 1203 74-75 0.44066134655403744 1203 76-77 0.1965704635745027 1203 78-79 -0.16561216863379258 1203 80-81 -0.782192940598371 1203 82-83 0.5756191112161559 1203 84-85 -0.04148643400765906 1203 86-87 -0.3262264108966235 1203 88-89 -0.1513284684347198 1203 90-91 -1.0237867420335647 1203 92-93 -0.16537988953417937 1204 1 -0.060042867397363864 1204 2 -0.005947430188335545 1204 3 0.046636958939558326 1204 4 0.045604018684379355 1204 5 -0.08337822085065483 1204 6 -0.04233276429187072 1204 7 0.09145635425913667 1204 8 0.09004142840373675 1204 9 -0.10455572903692456 1204 10-11 0.13703992542775012 1204 12-13 0.206297444363571 1204 14-15 0.2836221189682746 1204 16-17 0.41901183101116857 1204 18-19 0.29939925898938924 1204 20-21 0.32817462773431316 1204 22-23 0.443307709446664 1204 24-25 0.47083694242495255 1204 26-27 0.22801249352901465 1204 28-29 0.4735250084509701 1204 30-31 0.3706859194671921 1204 32-33 0.410824960875388 1204 34-35 0.4145707260039089 1204 36-37 0.3763055172873564 1204 38-39 0.56153856760411 1204 40-41 0.3169872700564582 1204 42-43 0.1987675421427042 1204 44-45 0.4518771031097941 1204 46-47 0.26419555069075074 1204 48-49 0.31767312656506874 1204 50-51 0.45773133736304317 1204 52-53 0.5024068752090542 1204 54-55 0.7508053158396066 1204 56-57 0.8653496362373545 1204 58-59 0.7346210791330563 1204 60-61 0.7930547822513248 1204 62-63 0.6886722604656086 1204 64-65 0.6856245395668168 1204 66-67 1.0453091128591794 1204 68-69 0.7830606921093519 1204 70-71 0.7449244543120273 1204 72-73 1.2276193916750486 1204 74-75 0.9428934894111762 1204 76-77 0.8392246843537237 1204 78-79 0.9226589352623122 1204 80-81 0.835096020440595 1204 82-83 0.6825590462810922 1204 84-85 0.4678479815767602 1204 86-87 0.760624238454028 1204 88-89 0.5883225055912575 1204 90-91 0.36257689433007556 1204 92-93 0.46915744812815774 1316 1 0.16722985987536276 1316 2 -0.1691395012751471 1316 3 0.14403955634215748 1316 4 0.15599362907398984 1316 5 0.04375780102220972 1316 6 0.23039450843540266 1316 7 0.08872224626187375 1316 8 0.28484662320893506 1316 9 0.2785611540799593 1316 10-11 0.15190663766288282 1316 12-13 0.1854498708844119 1316 14-15 0.12145533838044287 1316 16-17 -0.48520894820961047 1316 18-19 0.21677793294701075 1316 20-21 0.4054131786570707 1316 22-23 0.6992031298157713 1316 24-25 0.5600372158357487 1316 26-27 0.4662216527908072 1316 28-29 -1.1469466251785576 1316 30-31 0.0760857827617869 1316 32-33 0.14775250701345755 1316 34-35 0.42439642661909005 1316 36-37 0.7778776293857774 1316 38-39 0.580933646209715 1316 40-41 -0.16276324258879526 1316 42-43 -0.3174662240910635 1316 44-45 -0.45909350522923376 1316 46-47 0.5975004037324396 1316 48-49 0.6072322516505082 1316 50-51 1.11434446108143 1316 52-53 1.0364123434250487 1316 54-55 1.5378012146776072 1316 56-57 1.2614535323412497 1316 58-59 0.5642519745534287 1316 60-61 -0.013250754317375879 1316 62-63 0.41175838486752525 1316 64-65 0.7006621335517877 1316 66-67 -0.45947557613603607 1316 68-69 0.5648446975775627 1316 70-71 0.9424637571144885 1316 72-73 0.16225712236541057 1316 74-75 -0.10127944291964752 1316 76-77 0.8947612530481877 1316 78-79 1.4503417787346322 1316 80-81 0.999057059401629 1316 82-83 -0.4827690466785839 1316 84-85 -0.13852590769186435 1316 86-87 0.3612735891033765 1316 88-89 0.9366649526179103 1316 90-91 0.5222658895453804 1316 92-93 0.7104424788868755 1206 1 0.16722985987536276 1206 2 0.1466499724090653 1206 3 0.14403955634215748 1206 4 0.15599362907398984 1206 5 0.2542841168116823 1206 6 0.23039450843540266 1206 7 0.2992485620513463 1206 8 0.28484662320893506 1206 9 0.2785611540799593 1206 10-11 0.25716979555762265 1206 12-13 0.2907130287791517 1206 14-15 0.4297260150721698 1206 16-17 0.33621962321895893 1206 18-19 0.16790575249588358 1206 20-21 0.7437590433187253 1206 22-23 0.798827189966147 1206 24-25 0.2987590203470347 1206 26-27 0.4455449610614792 1206 28-29 0.27222630715227325 1206 30-31 0.2960105947918663 1206 32-33 0.7079028829533129 1206 34-35 0.7571031935363806 1206 36-37 0.1068249978068323 1206 38-39 0.3196554507209939 1206 40-41 0.7601690882382783 1206 42-43 0.9325337759089365 1206 44-45 0.9356433368760264 1206 46-47 1.1294552909504887 1206 48-49 1.186179620071563 1206 50-51 0.3229910776227811 1206 52-53 0.20370557650775822 1206 54-55 0.6355455755798616 1206 56-57 0.9043106751983885 1206 58-59 1.3612444557564345 1206 60-61 1.3401327043292426 1206 62-63 1.0019839487772941 1206 64-65 0.8999102538525392 1206 66-67 1.4803740479241085 1206 68-69 0.01033341938207144 1206 70-71 0.4105088698964465 1206 72-73 -0.2155624265067715 1206 74-75 0.31789348941117623 1206 76-77 0.9398740350030721 1206 78-79 0.4616199742233462 1206 80-81 -0.35808579774122506 1206 82-83 0.08677982550187124 1206 84-85 0.6772635659923409 1206 86-87 0.5041307319605224 1206 88-89 -0.01070346843471981 1206 90-91 1.529784686537866 1206 92-93 0.20292368189439003 1207 1 -0.0536712665326462 1207 2 -0.06361285612660339 1207 3 -0.046824023132188586 1207 4 -0.040501990450415803 1207 5 -0.11805630872023443 1207 6 -0.14382326377986487 1207 7 -0.06433091229158094 1207 8 -0.0011358548886875042 1207 9 -0.006169759562091315 1207 10-11 -0.07261743848493296 1207 12-13 -0.11135205257254199 1207 14-15 -0.1867588767569046 1207 16-17 -0.022442990762804982 1207 18-19 -0.16402702061336072 1207 20-21 -0.1708663813192146 1207 22-23 0.05173260745228703 1207 24-25 0.03016434843571858 1207 26-27 -0.06236671424694151 1207 28-29 0.1378172243702238 1207 30-31 -0.1621066946771137 1207 32-33 -0.18124426526767934 1207 34-35 -0.25943533676936426 1207 36-37 -0.3277807593896611 1207 38-39 -0.0928691335808125 1207 40-41 -0.2161852832975697 1207 42-43 -0.21452504762047653 1207 44-45 -0.10972587463837158 1207 46-47 -0.2999207147709484 1207 48-49 -0.09823840245659454 1207 50-51 -0.10397119664862231 1207 52-53 -0.40053186314894873 1207 54-55 -0.02787298333306154 1207 56-57 0.01802424573298822 1207 58-59 -0.04886639709534535 1207 60-61 0.09558594945708165 1207 62-63 -0.034177324242548934 1207 64-65 -0.005104943715849686 1207 66-67 0.1652032987733847 1207 68-69 0.20446000618701277 1207 70-71 -0.010553171941566575 1207 72-73 0.3154584924991255 1207 74-75 0.2908060586942156 1207 76-77 0.22169953115898267 1207 78-79 0.31751126691063547 1207 80-81 0.07102201559687416 1207 82-83 -0.14315044447846503 1207 84-85 -0.1487689746834988 1207 86-87 -0.09147985269888181 1207 88-89 -0.31702386893534396 1207 90-91 -0.27738498984332693 1207 92-93 0.17310962861350276 1210 1 -0.16442843158192488 1210 2 -0.05938017834470344 1210 3 -0.12229210194929863 1210 4 -0.10531290358932921 1210 5 -0.12260030529886734 1210 6 -0.09121353176560376 1210 7 -0.08266098568734748 1210 8 -0.1573644320674461 1210 9 0.017254621416640248 1210 10-11 -0.05438799338710254 1210 12-13 -0.10627189584396035 1210 14-15 -0.2422050689192119 1210 16-17 -0.27917879745584173 1210 18-19 -0.2875859919405812 1210 20-21 -0.4263773529483217 1210 22-23 -0.20135227880629003 1210 24-25 -0.26567026895662593 1210 26-27 -0.2929690375027647 1210 28-29 -0.16585696635813463 1210 30-31 -0.3879448969525683 1210 32-33 -0.04981427426132967 1210 34-35 -0.11421769662873515 1210 36-37 -0.33664233887657957 1210 38-39 -0.1995477079294048 1210 40-41 -0.5605416081005643 1210 42-43 -0.5636973798699572 1210 44-45 -0.2125978691541235 1210 46-47 -0.4784054412821064 1210 48-49 -0.04748872163698081 1210 50-51 0.1160635830068486 1210 52-53 -0.43251122176933876 1210 54-55 -0.1368880209743324 1210 56-57 -0.08528013600046336 1210 58-59 0.15629111763726655 1210 60-61 0.22742631524094747 1210 62-63 -0.2589636463411509 1210 64-65 0.28899855248857165 1210 66-67 0.10743793880710228 1210 68-69 0.13506421622342657 1210 70-71 0.010831913686828898 1210 72-73 0.23974984915726338 1210 74-75 0.29689564303645 1210 76-77 0.11718918216746488 1210 78-79 0.4041899670446014 1210 80-81 -0.11903339285967718 1210 82-83 0.03957953835183048 1210 84-85 -0.12173140888203449 1210 86-87 -0.4176208832584365 1210 88-89 -0.3850753277312009 1210 90-91 -0.5319525711792892 1210 92-93 -0.05694710058945418 1211 1 0.16722985987536276 1211 2 0.1466499724090653 1211 3 0.14403955634215748 1211 4 0.15599362907398984 1211 5 0.2542841168116823 1211 6 0.23039450843540266 1211 7 0.2992485620513463 1211 8 0.28484662320893506 1211 9 0.2785611540799593 1211 10-11 0.25716979555762265 1211 12-13 0.2907130287791517 1211 14-15 0.8582974436436004 1211 16-17 1.0147910517903895 1211 18-19 0.9536200382101683 1211 20-21 0.9580447576044406 1211 22-23 -0.3797442386052836 1211 24-25 -0.3083838367958265 1211 26-27 1.1241163896329098 1211 28-29 1.3793691642951273 1211 30-31 1.2602963090775816 1211 32-33 1.2793314543818823 1211 34-35 1.2928174792506653 1211 36-37 1.3568249978068323 1211 38-39 -1.2874874064218673 1211 40-41 1.6530262310954171 1211 42-43 1.6825337759089365 1211 44-45 1.9356433368760264 1211 46-47 1.8080267195219193 1211 48-49 0.6861796200715631 1211 50-51 -0.5698660652343577 1211 52-53 2.1679912907934735 1211 54-55 1.0641170041512922 1211 56-57 -0.23854646765875032 1211 58-59 -0.06732697281499611 1211 60-61 0.1972755614721038 1211 62-63 0.35912680592015533 1211 64-65 0.542767396709678 1211 66-67 0.9089454764955391 1211 68-69 1.1174762765249326 1211 70-71 1.231937441325016 1211 72-73 -0.1798481407924868 1211 74-75 1.9250363465540374 1211 76-77 -0.6315545364254973 1211 78-79 -1.6812371686337926 1211 80-81 1.999057059401629 1211 82-83 2.122494111216156 1211 84-85 2.177263565992341 1211 86-87 2.3612735891033765 1211 88-89 2.48929653156528 1211 90-91 2.6012132579664353 1211 92-93 -0.8685048895341794 1212 1 -0.08277014012463724 1212 2 0.1466499724090653 1212 3 -0.10596044365784252 1212 4 -0.010673037592674461 1212 5 0.08761745014501798 1212 6 0.23039450843540266 1212 7 0.2992485620513463 1212 8 0.28484662320893506 1212 9 0.2785611540799593 1212 10-11 0.09050312889095835 1212 12-13 -0.12595363788751968 1212 14-15 0.12913077697692898 1212 16-17 -0.23520894820961047 1212 18-19 0.14112003821016827 1212 20-21 -0.5419552423955594 1212 22-23 -0.671410905271955 1212 24-25 -0.03755050346249078 1212 26-27 -0.5633836103670902 1212 28-29 0.15020249762846305 1212 30-31 0.1561296424109173 1212 32-33 -0.24150187895144626 1212 34-35 -0.33218252074933474 1212 36-37 -0.3306750021931677 1212 38-39 0.4000125935781327 1212 40-41 -0.013640435571254272 1212 42-43 0.4950337759089365 1212 44-45 -0.6060233297906379 1212 46-47 -0.1711399471447521 1212 48-49 -0.6263203799284369 1212 50-51 0.01346726809897092 1212 52-53 -0.8736753758731979 1212 54-55 -1.7900496625153792 1212 56-57 -1.134379800992086 1212 58-59 -1.9423269728149961 1212 60-61 -1.5943911051945676 1212 62-63 -1.5367065274131804 1212 64-65 -1.7488992699569934 1212 66-67 -1.8827211901711323 1212 68-69 -2.1741903901417388 1212 70-71 -1.955562558674984 1212 72-73 -0.8465148074591582 1212 74-75 -2.7624636534459626 1212 76-77 -1.3607212030921687 1212 78-79 -0.6395705019671212 1212 80-81 -0.6467762739316996 1212 82-83 -0.9191725554505084 1212 84-85 -0.9060697673409877 1212 86-87 -1.3262264108966235 1212 88-89 -1.1148701351013912 1212 90-91 -0.8779534087002361 1212 92-93 0.40232844379915633 1213 1 -0.09914109845311003 1213 2 -0.14073184152813667 1213 3 -0.14719424778997592 1213 4 -0.19045203451886294 1213 5 -0.25954802979740066 1213 6 -0.3119190202210689 1213 7 -0.31940440865018616 1213 8 -0.258634175203575 1213 9 -0.3581844978401989 1213 10-11 -0.39731835665435966 1213 12-13 -0.3229790454592063 1213 14-15 -0.8023188747875878 1213 16-17 -0.7589337060011374 1213 18-19 -0.888039819382918 1213 20-21 -0.9719774205209291 1213 22-23 -0.9057159906771943 1213 24-25 -0.8112436476981202 1213 26-27 -0.9867158737031474 1213 28-29 -0.8211677797926455 1213 30-31 -1.0385831119566191 1213 32-33 -1.0744680086740246 1213 34-35 -1.1351619768016263 1213 36-37 -1.1325528474132014 1213 38-39 -0.8661030244912027 1213 40-41 -1.1185390776474335 1213 42-43 -1.2335978921195476 1213 44-45 -1.101184023927054 1213 46-47 -1.3122604288380657 1213 48-49 -1.10668836171903 1213 50-51 -1.0628624466981123 1213 52-53 -1.383426941960117 1213 54-55 -1.1034445693283388 1213 56-57 -1.1103802484454945 1213 58-59 -1.0848943826434123 1213 60-61 -1.0903397064163087 1213 62-63 -1.1567480627619986 1213 64-65 -1.3291247475648618 1213 66-67 -1.2724482435931748 1213 68-69 -1.399215680986451 1213 70-71 -1.2751829041868348 1213 72-73 -1.0437444872381505 1213 74-75 -1.431681290891973 1213 76-77 -1.3780819089012724 1213 78-79 -1.2547167997765527 1213 80-81 -1.4515674299178514 1213 82-83 -1.4727551008767534 1213 84-85 -1.5278840936317977 1213 86-87 -1.5318045012433004 1213 88-89 -1.625621175916919 1213 90-91 -1.7745892399908456 1213 92-93 -1.3348291570724058 1214 1 -0.3227247739548389 1214 2 -0.2616455557827706 1214 3 -0.28888332116918747 1214 4 -0.3605326184956823 1214 5 -0.4566685727540971 1214 6 -0.48120626927036625 1214 7 -0.5115097010724412 1214 8 -0.5006362024553539 1214 9 -0.6786650286808964 1214 10-11 -0.5253966334767242 1214 12-13 -0.6626246251417811 1214 14-15 -1.3176584863628804 1214 16-17 -1.082746213277666 1214 18-19 -1.1899314847969578 1214 20-21 -1.382849604028742 1214 22-23 -1.1610144913596585 1214 24-25 -1.1693689307686057 1214 26-27 -1.2760780368090892 1214 28-29 -1.2009937650632594 1214 30-31 -1.2614146436119853 1214 32-33 -1.3178817666161748 1214 34-35 -1.3581870573663153 1214 36-37 -1.4247692990175338 1214 38-39 -1.214577490673328 1214 40-41 -1.3557229587944093 1214 42-43 -1.3897280516996204 1214 44-45 -1.72086994893084 1214 46-47 -1.550041977820925 1214 48-49 -1.186795104490976 1214 50-51 -1.187818106711994 1214 52-53 -1.3815874519155287 1214 54-55 -1.8506594054404175 1214 56-57 -1.4750986387540195 1214 58-59 -1.5488564608253625 1214 60-61 -1.6731068105304914 1214 62-63 -1.6664726756093344 1214 64-65 -2.028522298688898 1214 66-67 -1.4718063057468456 1214 68-69 -1.57338568070125 1214 70-71 -1.9245758444818506 1214 72-73 -1.7255383546615732 1214 74-75 -1.7084698102832903 1214 76-77 -1.5716063834766985 1214 78-79 -1.4255663974089074 1214 80-81 -1.6542157857053112 1214 82-83 -1.7333062776367285 1214 84-85 -1.703812260320042 1214 86-87 -1.654280526256315 1214 88-89 -1.6918441035610954 1214 90-91 -1.6612624387283148 1214 92-93 -1.358459523364381 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 5.0 15 5.0 16 18.0 17 45.0 18 74.0 19 137.0 20 217.0 21 307.0 22 415.0 23 562.0 24 714.0 25 941.0 26 1099.0 27 1372.0 28 1792.0 29 2162.0 30 2770.0 31 3615.0 32 4708.0 33 6383.0 34 9025.0 35 14659.0 36 28335.0 37 61489.0 38 75518.0 39 1497.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.24190401872805 23.424754997590142 11.195978976842396 25.13736200683941 2 15.942441420145503 22.21375622518532 37.72703279553852 24.116769559130656 3 15.611043536134762 26.377802767769033 31.27579005347348 26.735363642622723 4 11.787574874348794 16.907259082459323 41.09746861588599 30.207697427305902 5 12.288802698919056 37.940467720836295 37.55949785417575 12.211231726068895 6 29.657815619764534 41.108943611869734 17.664149817547564 11.569090950818167 7 27.112202510729123 32.37142267000206 25.131159204094278 15.385215615174536 8 24.057099580015148 36.966929061574824 22.30555619305533 16.670415165354694 9 24.16909554081656 14.637045877033945 24.534000413099854 36.65985816904964 10-11 23.053725931195924 26.674775663828516 30.931081174121587 19.34041723085397 12-13 22.399192160282745 24.975099258715257 31.147958598214494 21.477749982787504 14-15 20.429623849631653 26.323181786886373 26.576549698207607 26.670644665274367 16-17 20.697220755972737 30.87049319532738 27.60241433915498 20.8298717095449 18-19 20.754595735891492 29.55729465494687 29.355334725632847 20.33277488352879 20-21 21.0311431390999 29.45769168980791 28.887843389254815 20.623321781837376 22-23 21.044224634521377 28.94705436853097 28.959906364032772 21.048814632914876 24-25 20.528767814931264 28.63378697817456 29.49969017510844 21.337755031785736 26-27 21.115369609620636 29.396644711174353 28.877515892869436 20.610469786335575 28-29 21.079338122231658 29.396185711335 28.76597893190737 20.75849723452597 30-31 20.775250728662247 28.84423840451656 29.129736304592292 21.2507745622289 32-33 20.88660612444891 29.251667894494055 29.088493077119036 20.773232903938 34-35 20.78663934068809 29.119150293647166 29.153805020115808 20.940405345548932 36-37 20.90561707498996 29.142234207355557 29.107349819266737 20.844798898387744 38-39 20.9119951529172 29.00653162767427 29.250492281846846 20.83098093756168 40-41 20.703100794763692 29.161837572608533 29.32409513296169 20.810966499666076 42-43 20.714852912151212 29.143085333443498 29.34871914919009 20.793342605215205 44-45 20.64095325961728 28.899124679270916 29.331735999228876 21.128186061882925 46-47 20.521382703809273 28.973483335857857 29.54563188792957 20.9595020724033 48-49 20.710721875674164 29.2541643142709 29.257377342641938 20.777736467413007 50-51 20.60905247793338 29.129544713879824 29.416651749034944 20.844751059151854 52-53 20.6391505536275 28.9320865238069 29.680585394516978 20.748177528048622 54-55 20.53645249600382 29.2194131589101 29.491796351075756 20.752337994010325 56-57 20.629964317799054 29.100868274548457 29.29846449356778 20.970702914084704 58-59 20.546005741380462 28.934213157368525 29.481230190743464 21.038550910507553 60-61 20.462094759079488 28.840349501975776 29.68031281392307 21.017242925021666 62-63 20.435311401461767 29.26798987476808 29.253045731377874 21.043652992392282 64-65 20.561504027001487 29.028898305279593 29.169377774814397 21.24021989290452 66-67 20.53029919855068 28.838841094164547 29.372680831888765 21.258178875396013 68-69 20.41487727945707 29.321197686287853 29.205328159053916 21.058596875201165 70-71 20.63339885735306 29.13312871446471 29.16991412904783 21.063558299134392 72-73 20.600271283076992 29.034416166632184 29.016483895441063 21.34882865484976 74-75 20.359172675103 29.15018944967628 29.2023800765156 21.28825779870512 76-77 20.548924752197514 29.23643955366812 28.971795007392114 21.24284068674225 78-79 20.644410883772036 29.197555128001785 28.87482099059152 21.283212997634664 80-81 20.61720877328033 29.282259269046495 28.823373967393177 21.277157990279996 82-83 20.49893633151537 29.394237351993862 28.94786416287223 21.15896215361854 84-85 20.454451502666355 29.42727352064415 28.856797710747635 21.26147726594186 86-87 20.254495692584634 29.126255946804797 29.014437648096102 21.604810712514467 88-89 20.308479159636473 29.358547912061173 28.931341693811824 21.40163123449053 90-91 20.658765096604498 29.37589225465579 28.793832368588962 21.17151028015075 92-93 20.611941394631515 29.15626262255352 28.761520948848823 21.470275033966143 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.5 8 0.5 9 1.5 10 2.5 11 8.5 12 9.0 13 13.0 14 23.0 15 30.5 16 55.5 17 91.0 18 143.5 19 256.0 20 447.0 21 678.0 22 1019.0 23 1504.0 24 2029.5 25 2620.5 26 3385.5 27 4208.5 28 5008.5 29 5423.0 30 5839.0 31 6534.5 32 7217.5 33 7820.5 34 8283.0 35 8692.0 36 8933.5 37 8985.5 38 8920.5 39 8850.5 40 8651.5 41 8346.5 42 8146.5 43 8101.0 44 8066.0 45 7593.0 46 6891.0 47 6465.5 48 6057.5 49 5539.5 50 5102.5 51 4731.0 52 4281.0 53 3841.5 54 3468.0 55 3222.5 56 3022.5 57 2890.0 58 2716.0 59 2505.0 60 2315.5 61 2031.0 62 1769.0 63 1543.0 64 1397.0 65 1281.5 66 1129.5 67 965.5 68 832.5 69 758.5 70 651.0 71 562.0 72 496.0 73 429.0 74 364.5 75 288.0 76 266.5 77 228.0 78 200.5 79 177.5 80 137.0 81 114.0 82 93.5 83 67.5 84 41.0 85 21.0 86 14.0 87 12.0 88 8.0 89 5.5 90 3.5 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0045899983935005625 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 2.2949991967502811E-4 34-35 6.884997590250844E-4 36-37 0.0011474995983751406 38-39 9.179996787001125E-4 40-41 6.884997590250844E-4 42-43 9.179996787001125E-4 44-45 9.179996787001125E-4 46-47 9.179996787001125E-4 48-49 9.179996787001125E-4 50-51 9.179996787001125E-4 52-53 0.01331099534115163 54-55 0.07206297477795882 56-57 0.11452045991783902 58-59 0.14756844835104307 60-61 0.16317444288894498 62-63 0.17832143758749686 64-65 0.18153443646294726 66-67 0.1762559383104216 68-69 0.1739609391136713 70-71 0.17786243774814678 72-73 0.17441993895302138 74-75 0.1803869368645721 76-77 0.18520643517774768 78-79 0.1599614440134946 80-81 0.12507745622289032 82-83 0.1018979643357125 84-85 0.06976797558120855 86-87 0.04635898377435568 88-89 0.025015491244578067 90-91 0.008261997108301012 92-93 4.5899983935005623E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 93 217865.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.7184046021506 #Duplication Level Percentage of deduplicated Percentage of total 1 97.11085368904564 92.95295984668319 2 2.2552895988245747 4.317454446306251 3 0.40425883307131893 1.1608503164374129 4 0.11324033075347502 0.4335673518536992 5 0.05871566728971415 0.2810084999061059 6 0.021355126291079194 0.12264471711957252 7 0.01035470446330085 0.06937950539477478 8 0.007379710711021035 0.05650993085474687 9 0.005008416672606793 0.043145788813626046 >10 0.011123329617997965 0.14496095909280643 >50 4.965757817364806E-4 0.04325361184861233 >100 0.0019240174775385546 0.37426502568916187 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAAC 280 0.12851995501801575 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10-11 0.0 0.0 0.0 12-13 0.0 0.0 0.0 14-15 0.0 0.0 2.2949991967502811E-4 16-17 0.0 0.0 4.5899983935005623E-4 18-19 0.0 0.0 4.5899983935005623E-4 20-21 0.0 2.2949991967502811E-4 4.5899983935005623E-4 22-23 0.0 4.5899983935005623E-4 4.5899983935005623E-4 24-25 0.0 4.5899983935005623E-4 0.001835999357400225 26-27 0.0 4.5899983935005623E-4 0.0043604984738255335 28-29 0.0 4.5899983935005623E-4 0.01698299405595208 30-31 0.0 4.5899983935005623E-4 0.02799899020035343 32-33 0.0 4.5899983935005623E-4 0.040621485782479975 34-35 0.0 4.5899983935005623E-4 0.06770247630413329 36-37 0.0 4.5899983935005623E-4 0.13425745300989145 38-39 0.0 4.5899983935005623E-4 0.252908911481881 40-41 0.0 4.5899983935005623E-4 0.39565786151974847 42-43 0.0 4.5899983935005623E-4 0.5457508089872168 44-45 0.0 4.5899983935005623E-4 0.7204002478599132 46-47 0.0 4.5899983935005623E-4 0.9241961765313382 48-49 0.0 4.5899983935005623E-4 1.1199596080141372 50-51 4.5899983935005623E-4 4.5899983935005623E-4 1.3338535331512635 52-53 0.0013769995180501686 4.5899983935005623E-4 1.5227319670438115 54-55 0.0013769995180501686 4.5899983935005623E-4 1.7237738966791363 56-57 0.0013769995180501686 4.5899983935005623E-4 1.9512083170770889 58-59 0.0013769995180501686 4.5899983935005623E-4 2.189888233539118 60-61 0.0013769995180501686 4.5899983935005623E-4 2.427191150483097 62-63 0.0013769995180501686 4.5899983935005623E-4 2.6472815734514494 64-65 0.0013769995180501686 4.5899983935005623E-4 2.8673719964198012 66-67 0.0013769995180501686 4.5899983935005623E-4 3.1085764119982557 68-69 0.0013769995180501686 4.5899983935005623E-4 3.3665343217129875 70-71 0.0013769995180501686 4.5899983935005623E-4 3.607279737452092 72-73 0.001835999357400225 4.5899983935005623E-4 3.834714157850045 74-75 0.001835999357400225 4.5899983935005623E-4 4.0814265715007 76-77 0.001835999357400225 4.5899983935005623E-4 4.312762490533128 78-79 0.001835999357400225 4.5899983935005623E-4 4.57255639960526 80-81 0.001835999357400225 4.5899983935005623E-4 4.811924815826314 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACAGCGA 35 4.3118733E-4 49.677433 8 CAGCGAA 50 0.0024930458 34.774204 9 CCTAGCC 55 0.003976925 31.612913 3 TAGGTAC 55 0.003976925 31.612913 5 ACACAGC 55 0.003976925 31.612913 6 CGGTGAG 55 0.003976925 31.612913 9 TAGAGTG 70 3.446655E-4 31.048395 7 TTACACT 70 3.446655E-4 31.048395 4 CTGTGGA 90 4.4053508E-5 28.978502 9 ACCTAGG 80 7.5305026E-4 27.167345 1 TATACTG 80 7.5305026E-4 27.167345 5 TGTACTG 80 7.5305026E-4 27.167345 5 GTAGGAC 65 0.008995219 26.749386 3 ACTAGGG 65 0.008995219 26.749386 3 GTAGTCA 65 0.008995219 26.749386 6 CTCTAGG 100 9.027784E-5 26.080652 2 CTGGGCA 85 0.0010728425 25.569267 9 ATGCCGT 105 1.2914825E-10 24.850122 86-87 TATGCCG 80 1.4591205E-7 24.467457 84-85 GTATATA 160 1.461849E-7 24.450611 1 >>END_MODULE