FastQCFastQC Report
Wed 5 Nov 2014
DP2A_AGGCAGAA-TATCCTCT_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDP2A_AGGCAGAA-TATCCTCT_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217865
Sequences flagged as poor quality0
Sequence length93
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAAC2800.12851995501801575No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGCGA354.3118733E-449.6774338
CAGCGAA500.002493045834.7742049
CCTAGCC550.00397692531.6129133
TAGGTAC550.00397692531.6129135
ACACAGC550.00397692531.6129136
CGGTGAG550.00397692531.6129139
TAGAGTG703.446655E-431.0483957
TTACACT703.446655E-431.0483954
CTGTGGA904.4053508E-528.9785029
ACCTAGG807.5305026E-427.1673451
TATACTG807.5305026E-427.1673455
TGTACTG807.5305026E-427.1673455
GTAGGAC650.00899521926.7493863
ACTAGGG650.00899521926.7493863
GTAGTCA650.00899521926.7493866
CTCTAGG1009.027784E-526.0806522
CTGGGCA850.001072842525.5692679
ATGCCGT1051.2914825E-1024.85012286-87
TATGCCG801.4591205E-724.46745784-85
GTATATA1601.461849E-724.4506111
Produced by FastQC (version 0.11.2)