##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename DP18_GGACTCCT-CTCTCTAT_L006_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 42852012 Sequences flagged as poor quality 0 Sequence length 93 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.695643369090813 33.0 33.0 33.0 27.0 33.0 2 31.68948190810737 33.0 33.0 33.0 27.0 33.0 3 31.72533681732377 33.0 33.0 33.0 27.0 33.0 4 35.42401206272415 37.0 37.0 37.0 33.0 37.0 5 35.30313085415919 37.0 37.0 37.0 33.0 37.0 6 35.42851374166515 37.0 37.0 37.0 33.0 37.0 7 35.37387292339972 37.0 37.0 37.0 33.0 37.0 8 35.35261620854582 37.0 37.0 37.0 33.0 37.0 9 35.4454663645665 37.0 37.0 37.0 33.0 37.0 10-11 35.40628662896855 37.0 37.0 37.0 33.0 37.0 12-13 35.36369277129858 37.0 37.0 37.0 33.0 37.0 14-15 37.11647349253986 40.0 37.0 40.0 33.0 40.0 16-17 37.144347924666874 40.0 37.0 40.0 33.0 40.0 18-19 37.1839875336542 40.0 37.0 40.0 33.0 40.0 20-21 37.032685898155734 40.0 37.0 40.0 33.0 40.0 22-23 36.9535830429619 40.0 37.0 40.0 33.0 40.0 24-25 36.88668074675233 40.0 37.0 40.0 33.0 40.0 26-27 36.898263481770705 40.0 37.0 40.0 33.0 40.0 28-29 36.797082736745246 40.0 37.0 40.0 33.0 40.0 30-31 36.71644156405071 40.0 37.0 40.0 33.0 40.0 32-33 36.57782633403538 40.0 37.0 40.0 33.0 40.0 34-35 36.490003678240356 40.0 37.0 40.0 33.0 40.0 36-37 36.30932601251022 38.5 37.0 40.0 33.0 40.0 38-39 36.270684349663675 37.0 37.0 40.0 33.0 40.0 40-41 36.25011911926096 37.0 37.0 40.0 33.0 40.0 42-43 36.28205872807092 37.0 37.0 40.0 33.0 40.0 44-45 36.22245874942815 37.0 37.0 40.0 27.0 40.0 46-47 36.08248362994017 37.0 37.0 40.0 27.0 40.0 48-49 35.91580340498365 37.0 37.0 40.0 27.0 40.0 50-51 34.855162390041336 37.0 35.0 38.5 27.0 40.0 52-53 34.962626130600356 37.0 33.0 38.5 27.0 40.0 54-55 35.6339880843868 37.0 37.0 40.0 27.0 40.0 56-57 35.605868914626456 37.0 35.0 40.0 27.0 40.0 58-59 35.48100172052598 37.0 33.0 40.0 27.0 40.0 60-61 35.649709889934684 37.0 37.0 40.0 27.0 40.0 62-63 35.577983164477786 37.0 35.0 40.0 27.0 40.0 64-65 35.43765037216922 37.0 33.0 40.0 27.0 40.0 66-67 35.19825483340199 37.0 33.0 40.0 27.0 40.0 68-69 35.01990016244745 37.0 33.0 40.0 27.0 40.0 70-71 34.80149584341571 37.0 33.0 40.0 27.0 40.0 72-73 34.534389820482644 37.0 33.0 37.0 27.0 40.0 74-75 34.293843262248686 37.0 33.0 37.0 27.0 40.0 76-77 34.03403979024369 37.0 33.0 37.0 27.0 40.0 78-79 33.75597928750697 37.0 33.0 37.0 27.0 40.0 80-81 33.498180797205045 37.0 33.0 37.0 27.0 37.0 82-83 33.23634493521564 37.0 33.0 37.0 27.0 37.0 84-85 33.05359824178151 37.0 33.0 37.0 24.5 37.0 86-87 32.88528160591386 37.0 33.0 37.0 22.0 37.0 88-89 32.68657478673347 37.0 33.0 37.0 22.0 37.0 90-91 32.515786971682914 37.0 33.0 37.0 22.0 37.0 92-93 31.538659503315735 35.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2304 1 0.42845849499174804 2304 2 0.4769338494322213 2304 3 0.5229368325836639 2304 4 0.5775447488133878 2304 5 0.5175293400523273 2304 6 0.5803681501865512 2304 7 0.6036807323693196 2304 8 0.6860177296375696 2304 9 0.663143521314062 2304 10-11 0.6924929429340523 2304 12-13 0.7841128012569598 2304 14-15 1.0559492356468922 2304 16-17 1.0238786787844631 2304 18-19 1.0012137460870534 2304 20-21 1.0256857639847539 2304 22-23 1.1164278265753396 2304 24-25 1.1030125682887189 2304 26-27 0.9939505477197557 2304 28-29 1.1000440877777322 2304 30-31 1.0519908843135894 2304 32-33 1.0644788432987582 2304 34-35 1.0528156940210351 2304 36-37 1.0428312360508016 2304 38-39 1.0569018588177173 2304 40-41 1.0357550102815622 2304 42-43 0.9972181777803328 2304 44-45 1.0462221323936305 2304 46-47 1.0248588326075918 2304 48-49 1.0237493446216632 2304 50-51 0.9892258441334718 2304 52-53 1.0251391187857521 2304 54-55 1.1246292926477253 2304 56-57 1.2331273200651722 2304 58-59 1.061869093158137 2304 60-61 1.1257971291653774 2304 62-63 1.094001857262775 2304 64-65 1.0408166051887235 2304 66-67 1.0931473566214294 2304 68-69 1.116717137677277 2304 70-71 1.0620559795336035 2304 72-73 1.1133343803087854 2304 74-75 1.091434744470213 2304 76-77 1.1350427394818468 2304 78-79 1.1698689938126563 2304 80-81 1.0226917616510036 2304 82-83 0.9162999071987485 2304 84-85 1.0249101805316982 2304 86-87 1.0081583439997672 2304 88-89 1.0122686055628378 2304 90-91 0.9956109758191118 2304 92-93 0.9751943191608987 2305 1 0.3760911673808174 2305 2 0.34166548116199635 2305 3 0.34419131502184896 2305 4 0.4001084477891794 2305 5 0.41910400141009774 2305 6 0.4901618190830419 2305 7 0.4137934241443091 2305 8 0.4799411774856637 2305 9 0.47745419846513215 2305 10-11 0.508046475417693 2305 12-13 0.5460698232061816 2305 14-15 0.7994656236409625 2305 16-17 0.7880574116300352 2305 18-19 0.7358488483855652 2305 20-21 0.838561282813771 2305 22-23 0.7371099575147753 2305 24-25 0.7249887983053256 2305 26-27 0.6443250383391543 2305 28-29 0.7010182747140306 2305 30-31 0.6653072549670469 2305 32-33 0.6783480016786143 2305 34-35 0.7237772756631031 2305 36-37 0.7078682182499918 2305 38-39 0.7685826536404363 2305 40-41 0.7502249765562823 2305 42-43 0.7171077536244113 2305 44-45 0.6643013923494792 2305 46-47 0.6953717813794071 2305 48-49 0.7148821759663875 2305 50-51 0.6201268225444068 2305 52-53 0.5997567915416369 2305 54-55 0.7405947759284857 2305 56-57 0.7643213104300131 2305 58-59 0.7726534003449359 2305 60-61 0.8158977492035717 2305 62-63 0.7376129517002212 2305 64-65 0.6186212831534732 2305 66-67 0.7265245972847367 2305 68-69 0.6770512557846544 2305 70-71 0.6259979462707435 2305 72-73 0.7045740342967974 2305 74-75 0.5965737917589635 2305 76-77 0.5674024416678805 2305 78-79 0.7433751997524851 2305 80-81 0.6316843163017367 2305 82-83 0.4839255321834628 2305 84-85 0.5701385097633036 2305 86-87 0.6211855932222221 2305 88-89 0.6704663819622638 2305 90-91 0.6860531837686707 2305 92-93 0.7724666179708493 2306 1 0.5704193549165559 2306 2 0.3891135467232658 2306 3 0.4753710367676085 2306 4 0.4653419145320825 2306 5 0.6977317887171282 2306 6 0.5970190708459526 2306 7 0.7825450917639003 2306 8 0.1553785702815489 2306 9 0.5988149760523243 2306 10-11 0.5814083965154637 2306 12-13 0.6320217957619221 2306 14-15 0.6409159762233898 2306 16-17 0.6808342364823332 2306 18-19 0.8486580727042252 2306 20-21 0.7950716697015139 2306 22-23 0.9046780916866908 2306 24-25 0.9234965170017233 2306 26-27 0.7773381887107931 2306 28-29 0.8518053911615198 2306 30-31 0.9323379391675388 2306 32-33 0.9345858518787296 2306 34-35 0.8434837746148816 2306 36-37 1.0233113285987585 2306 38-39 0.8790362461694983 2306 40-41 1.0070593581076466 2306 42-43 0.9367323009848079 2306 44-45 0.7578234049280894 2306 46-47 0.7148000260906713 2306 48-49 0.6638148994274076 2306 50-51 0.6128197899543579 2306 52-53 0.6765263741602823 2306 54-55 0.8419993880878138 2306 56-57 0.9221333935251153 2306 58-59 0.9117080982675532 2306 60-61 1.3254327215687312 2306 62-63 1.14354586574634 2306 64-65 0.8849677668584448 2306 66-67 1.235137137784065 2306 68-69 0.9184215626266621 2306 70-71 0.9366592765547139 2306 72-73 0.9735734625415091 2306 74-75 0.9825848456945536 2306 76-77 0.9306023654338418 2306 78-79 1.0321344907759311 2306 80-81 0.9116824104507728 2306 82-83 1.3231041104186474 2306 84-85 1.0328943702550148 2306 86-87 1.0979761402014532 2306 88-89 0.9814212132944533 2306 90-91 0.6285079198083139 2306 92-93 0.7186282341324599 2307 1 0.5213068008040018 2307 2 0.6623602999700182 2307 3 0.7000463614429329 2307 4 0.6376795768697434 2307 5 0.8337491047344443 2307 6 0.8378632266901107 2307 7 0.8881944424132513 2307 8 0.8342313841343625 2307 9 0.6363041535415022 2307 10-11 0.9558564484635141 2307 12-13 0.972249068489198 2307 14-15 1.0599852402926544 2307 16-17 1.2505312061792964 2307 18-19 1.2883009298470895 2307 20-21 1.0382318428616912 2307 22-23 1.1539638059724027 2307 24-25 0.99287963388484 2307 26-27 0.8200438164164225 2307 28-29 0.9034179452740716 2307 30-31 0.9655955149251199 2307 32-33 1.2379841202769981 2307 34-35 1.3083647269958334 2307 36-37 1.2962442290316645 2307 38-39 1.1062548608881144 2307 40-41 1.2228926914409755 2307 42-43 1.0093621711146739 2307 44-45 1.2290138811185756 2307 46-47 1.1915532728439189 2307 48-49 1.0750161981287079 2307 50-51 0.6650167596513299 2307 52-53 1.190086980220883 2307 54-55 1.395689864278296 2307 56-57 0.9543520082437311 2307 58-59 1.3268163233757804 2307 60-61 1.0171967908328057 2307 62-63 1.4886649133653904 2307 64-65 1.5300543469450218 2307 66-67 1.4677345403814712 2307 68-69 1.5109648526699502 2307 70-71 1.6672004020958369 2307 72-73 1.7586059300739763 2307 74-75 1.324576187685885 2307 76-77 1.1307971706286395 2307 78-79 1.0586604648019033 2307 80-81 1.3016715879399499 2307 82-83 1.2996300844446225 2307 84-85 1.3596151494757933 2307 86-87 1.192846270071584 2307 88-89 1.0479147197879612 2307 90-91 0.8049473137477037 2307 92-93 1.172069792574021 2308 1 0.11921542371262106 2308 2 0.11687767448739805 2308 3 0.0934054348020048 2308 4 0.27787262706279847 2308 5 0.1354222064075472 2308 6 0.21077828460516912 2308 7 0.1308006200194285 2308 8 0.18825326315624125 2308 9 0.10505576229311231 2308 10-11 0.1361749812820534 2308 12-13 0.15853285227298386 2308 14-15 0.16167764698506204 2308 16-17 0.20854922419731992 2308 18-19 0.09109050513665551 2308 20-21 0.2597891144189646 2308 22-23 -0.06288947588087979 2308 24-25 -0.04834623484102707 2308 26-27 0.033898385270994424 2308 28-29 0.07625256560869786 2308 30-31 -0.07694953011993277 2308 32-33 -0.024750757457880468 2308 34-35 0.008348639479748954 2308 36-37 0.02232852761596149 2308 38-39 -0.07248709535384279 2308 40-41 -0.07510280405450942 2308 42-43 -0.12545443120192346 2308 44-45 -0.21541983831514955 2308 46-47 -0.08728842599778375 2308 48-49 -0.1056691300566186 2308 50-51 -0.10000576287119145 2308 52-53 -0.10615175851785352 2308 54-55 0.0722696583580813 2308 56-57 0.054123565515290295 2308 58-59 -0.049888953329492836 2308 60-61 -0.018842462706444962 2308 62-63 0.0738161360166103 2308 64-65 0.03504147693215742 2308 66-67 -0.17697588432895373 2308 68-69 0.028053012258105525 2308 70-71 0.03380915370458837 2308 72-73 -0.009473220505171298 2308 74-75 -0.06707117396147311 2308 76-77 -0.1211912463597713 2308 78-79 -0.09727582863439466 2308 80-81 0.034679953448311096 2308 82-83 -0.12171161439707845 2308 84-85 -0.10290415554350574 2308 86-87 -0.11821550599019304 2308 88-89 -0.17629377442053595 2308 90-91 -0.16475989345950381 2308 92-93 0.14595010145433207 2309 1 0.42730296591557604 2309 2 0.3572602003233101 2309 3 0.3824904234050628 2309 4 0.5350208215477039 2309 5 0.7047670109457584 2309 6 0.5607661164446185 2309 7 0.5487501992752826 2309 8 0.5441651039761339 2309 9 0.48138371992734363 2309 10-11 0.45056271714727814 2309 12-13 0.4740325180770242 2309 14-15 0.4452141257593283 2309 16-17 0.4400260275701342 2309 18-19 0.3889497657923684 2309 20-21 0.5440219204653403 2309 22-23 0.5570687067786366 2309 24-25 0.39303476569025975 2309 26-27 0.44591949960028643 2309 28-29 0.528770860695154 2309 30-31 0.47905725662877074 2309 32-33 0.4915132744878292 2309 34-35 0.45217115571824706 2309 36-37 0.5118868748027197 2309 38-39 0.5676625216061737 2309 40-41 0.4868564411767622 2309 42-43 0.25200756471917174 2309 44-45 0.471038370051744 2309 46-47 0.4314980142154212 2309 48-49 0.49673125384028083 2309 50-51 0.47558799194894164 2309 52-53 0.4244850990118394 2309 54-55 0.5444597558747546 2309 56-57 0.5989397577379449 2309 58-59 0.527415713417156 2309 60-61 0.62936539004167 2309 62-63 0.550787463717171 2309 64-65 0.6089073529540201 2309 66-67 0.5232422856482515 2309 68-69 0.3826678299927764 2309 70-71 0.5253812457712925 2309 72-73 0.38700863865265234 2309 74-75 0.4009568984990679 2309 76-77 0.5060009934386827 2309 78-79 0.5461876411765729 2309 80-81 0.44744075483308876 2309 82-83 0.541596068682324 2309 84-85 0.41036136743031904 2309 86-87 0.4862265581411549 2309 88-89 0.5486847170703228 2309 90-91 0.23113892228712274 2309 92-93 0.4054582335559971 2310 1 -0.08121700871980764 2310 2 -0.009068271458552601 2310 3 -0.021810781414213665 2310 4 -0.11874899455882826 2310 5 0.15836815235349633 2310 6 0.0552917981186809 2310 7 -0.026091271872466848 2310 8 -0.01053052062754034 2310 9 -0.11745775122039959 2310 10-11 0.012499305606375799 2310 12-13 0.013749068489197214 2310 14-15 -0.22703856923116206 2310 16-17 -0.34930212715403286 2310 18-19 -0.2553419272957669 2310 20-21 -0.24329196666212027 2310 22-23 -0.3673219083133148 2310 24-25 -0.6090489375437329 2310 26-27 -0.4674799931073821 2310 28-29 -0.6850582452021143 2310 30-31 -0.5915711517415545 2310 32-33 -0.5932777844849113 2310 34-35 -0.6155162253851145 2310 36-37 -1.0842795804921437 2310 38-39 -0.9715546629214131 2310 40-41 -0.8939406418923568 2310 42-43 -0.7549949717424624 2310 44-45 -0.8761765950719109 2310 46-47 -0.9110181557275183 2310 48-49 -1.1379123732998622 2310 50-51 -1.0793165736820072 2310 52-53 -1.2682463531124455 2310 54-55 -0.7935006119121866 2310 56-57 -0.5409575155657933 2310 58-59 -0.7970646290051704 2310 60-61 -0.7656127329767202 2310 62-63 -0.7069541342536638 2310 64-65 -0.6793504149597354 2310 66-67 -0.7354083167613936 2310 68-69 -0.8679875282824341 2310 70-71 -0.7157043598089246 2310 72-73 -0.6136083556403094 2310 74-75 -0.501233336123633 2310 76-77 -0.6084885436570744 2310 78-79 -0.7064109637695282 2310 80-81 -0.6980903168219541 2310 82-83 -0.6729413441268051 2310 84-85 -0.6452419933813474 2310 86-87 -0.6572965870712721 2310 88-89 -0.8484424230691872 2310 90-91 -0.5927193529189552 2310 92-93 -0.7576444931402619 2311 1 0.20819790523812642 2311 2 -0.08306444736677321 2311 3 0.037112736750223974 2311 4 0.028488099131422473 2311 5 0.11463775082386007 2311 6 0.16192086121619553 2311 7 0.32854543940860736 2311 8 0.05112339906653318 2311 9 0.020261178033898375 2311 10-11 0.07937119853180974 2311 12-13 0.09736857135726495 2311 14-15 -0.1427030051776157 2311 16-17 -0.18844266252688158 2311 18-19 -0.12273772079480949 2311 20-21 -0.11115812344988285 2311 22-23 -0.21256856223300247 2311 24-25 -0.2046244633563532 2311 26-27 -0.28178400840375417 2311 28-29 -0.30821025285030146 2311 30-31 -0.3087432358715674 2311 32-33 -0.21194986861492282 2311 34-35 -0.1854837206050064 2311 36-37 -0.18285415028181973 2311 38-39 -0.2181043761145247 2311 40-41 -0.20448939906252406 2311 42-43 -0.30737164478262 2311 44-45 -0.3482760214581404 2311 46-47 -0.4134043889971082 2311 48-49 -0.3361590272195514 2311 50-51 -0.20691600006824018 2311 52-53 -0.21697962271091598 2311 54-55 -0.13463541114736444 2311 56-57 -0.12646038363462964 2311 58-59 -0.3076869999420708 2311 60-61 -0.13268825878934365 2311 62-63 -0.10018262373740328 2311 64-65 -0.08820796754099547 2311 66-67 -0.26608135691435564 2311 68-69 -0.269007604764262 2311 70-71 -0.1819414534991708 2311 72-73 -0.25045921606096044 2311 74-75 -0.4650077147087188 2311 76-77 -0.19175814212743347 2311 78-79 -0.42237272285174754 2311 80-81 -0.5303130701680416 2311 82-83 -0.39077212806562756 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-0.7369881463366781 2303 84-85 -0.7274509881366313 2303 86-87 -0.7169072444762179 2303 88-89 -0.7402613272339025 2303 90-91 -0.7507937522260804 2303 92-93 -0.8857448076483152 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 86684.0 3 4214.0 4 2882.0 5 2192.0 6 2530.0 7 4416.0 8 8648.0 9 13624.0 10 21752.0 11 38681.0 12 63424.0 13 88278.0 14 105970.0 15 119772.0 16 131406.0 17 143909.0 18 158660.0 19 175584.0 20 193060.0 21 213922.0 22 237382.0 23 265816.0 24 300031.0 25 341928.0 26 393051.0 27 457254.0 28 538687.0 29 640215.0 30 781816.0 31 984934.0 32 1270131.0 33 1687316.0 34 2316488.0 35 3355672.0 36 5348597.0 37 9697335.0 38 1.2081938E7 39 573813.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.21401181184093 24.014065991156183 11.365702055262199 24.406220141740683 2 15.35502551050199 22.618770853751403 39.35596482187907 22.67023881386753 3 15.136668035013672 27.614695840475257 32.086273121812404 25.16236300269867 4 11.22126241160231 17.205194355436202 42.98455708210276 28.58898615085873 5 11.875277377606611 38.6432102957689 38.19031989034974 11.291192436274745 6 28.162989108594083 42.33729255056849 18.495929542507135 11.003788798330284 7 25.60706151197331 33.067804320277475 26.557948688616168 14.767185479133055 8 23.025073022900393 38.05260550346356 23.044957780690684 15.877363692945364 9 23.60290190214052 15.300498462887917 25.233538365843998 35.86306126912756 10-11 22.280759395377526 27.39109110869855 31.958417345817335 18.36973215010659 12-13 21.812386973668264 25.65149331426345 31.916538978837174 20.619580733231107 14-15 19.656932444953412 27.293931410228456 27.537445703427437 25.511690441390694 16-17 19.70844925998944 31.878416616630577 28.647627463611276 19.765506659768707 18-19 19.77865122151336 30.62308841097801 30.20249335580428 19.39576701170435 20-21 20.153189874345152 30.172890567719918 29.95516908568768 19.718750472247255 22-23 20.17094710293969 29.956709272128297 29.984188152089008 19.888155472843007 24-25 19.749131330049998 29.84932668510415 30.27488265311141 20.126659331734444 26-27 19.989001827870993 30.409154154391167 29.960142239657912 19.64170177807993 28-29 20.006467803729176 30.262910958459504 29.99257196718192 19.7380492706294 30-31 19.80553084596974 30.147987402919767 30.149285530852048 19.897196220258444 32-33 20.025080818400312 30.3645196200316 29.982933601333595 19.627465960234492 34-35 19.86402022756774 30.06130904588681 30.15726506094537 19.91740566560008 36-37 19.87779437076254 30.30610326218257 30.119312310845213 19.696790056209668 38-39 19.868184366576465 30.310970669464144 30.161567391287363 19.659277572672025 40-41 19.7481210360702 30.211327165648385 30.28981300789025 19.75073879039117 42-43 19.745752753787286 30.232359586973107 30.31713837577113 19.704749283468477 44-45 19.72571002723828 30.105267420226085 30.292889225624283 19.876133326911354 46-47 19.653135069229823 30.07377583094027 30.45834567387499 19.814743425954916 48-49 19.7179487425374 30.322350910055267 30.28888793096976 19.67081241643757 50-51 19.639519037151715 30.26370050095053 30.395972746333737 19.700807715564018 52-53 19.71243640688837 30.11342868554408 30.533508940390973 19.64062596717658 54-55 19.710417223391282 30.200053562537477 30.44165581583705 19.647873398234193 56-57 19.682187455529153 30.208548925188992 30.349496432401473 19.759767186880385 58-59 19.722442212567326 30.241940931119586 30.308206073868483 19.727410782444608 60-61 19.53243826262449 30.175309339274957 30.493574018689944 19.79867837941061 62-63 19.548096998454874 30.278646871135862 30.397604164606832 19.775651965802428 64-65 19.594285322152007 30.15234955851812 30.484698245093405 19.768666874236466 66-67 19.576810322715616 30.106095705091896 30.43044537194043 19.886648600252062 68-69 19.536808738786267 30.267168225859702 30.366245419799437 19.829777615554594 70-71 19.676484993845275 30.178325282482373 30.346406763711375 19.79878295996098 72-73 19.6189013750425 30.204983778260047 30.292437918351204 19.883676928346244 74-75 19.587111364092145 30.2709365838316 30.335178153753084 19.806773898323172 76-77 19.63588016579924 30.239122040147375 30.30530336230471 19.819694431748676 78-79 19.729938280357757 30.199296625734757 30.268109325068014 19.802655768839475 80-81 19.654279080354854 30.326556130264066 30.186332409851413 19.832832379529673 82-83 19.783976975999256 30.298137411684568 30.208665560831122 19.70922005148505 84-85 19.720066430830443 30.31414799437546 30.244548310343806 19.721237264450295 86-87 19.634128448543198 30.22492082482645 30.277141907957212 19.86380881867314 88-89 19.751605689612 30.2553027216708 30.179887311562194 19.813204277155005 90-91 19.848372797240206 30.327713967135445 30.13284930796143 19.69106392766292 92-93 19.935791428977602 30.21186753979291 30.11173108511968 19.74060994610981 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 82333.0 1 42768.5 2 2794.0 3 2393.0 4 2371.5 5 2548.0 6 2904.0 7 3393.0 8 4226.0 9 5374.5 10 6900.5 11 8867.0 12 11691.5 13 16008.5 14 18597.0 15 22648.0 16 32614.0 17 47167.5 18 69033.5 19 101457.0 20 147244.5 21 210132.0 22 295285.5 23 402318.0 24 526717.0 25 671156.0 26 838213.5 27 1017910.5 28 1197513.0 29 1286261.0 30 1368259.0 31 1521011.5 32 1651515.0 33 1758896.0 34 1843959.0 35 1905416.5 36 1932588.0 37 1925944.5 38 1890181.5 39 1830459.0 40 1755009.0 41 1664808.5 42 1568758.5 43 1521207.0 44 1467848.5 45 1349751.0 46 1229781.0 47 1121524.5 48 1014590.5 49 913976.0 50 823836.0 51 742855.5 52 667268.5 53 597331.0 54 536701.5 55 480589.0 56 429534.5 57 405860.0 58 386551.0 59 349731.0 60 314844.5 61 282619.0 62 252668.5 63 224059.0 64 199740.0 65 177062.0 66 154453.0 67 133107.0 68 114242.0 69 95866.5 70 78240.5 71 70730.0 72 64708.0 73 53119.0 74 43145.0 75 36729.5 76 32885.5 77 27921.5 78 22949.5 79 18573.0 80 15392.0 81 13447.5 82 10874.0 83 7988.0 84 5471.0 85 3808.0 86 3053.0 87 2419.5 88 1421.5 89 884.0 90 582.5 91 387.0 92 268.5 93 194.5 94 144.0 95 98.0 96 71.0 97 61.5 98 44.5 99 22.0 100 16.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.46055480428783596 2 0.48540544607333724 3 0.4941564937487649 4 0.48689895820994356 5 0.5042703712488459 6 0.2664682349104168 7 0.2884321977693836 8 0.270141341321383 9 0.26468768841005647 10-11 0.25345951083930435 12-13 0.23936098963101196 14-15 0.22450637790356262 16-17 0.23616860743901594 18-19 0.24354165680715295 20-21 0.24334446653286665 22-23 0.24418106669063752 24-25 0.23541135011350225 26-27 0.2342072059533634 28-29 0.23755477339080366 30-31 0.22901258405322952 32-33 0.22828566369299066 34-35 0.2348862872529766 36-37 0.22947580617684885 38-39 0.2449313231780109 40-41 0.24607712702031356 42-43 0.23511731491160787 44-45 0.24032243806895226 46-47 0.2569330933632708 48-49 0.22706285063114423 50-51 0.23961768702949116 52-53 0.22656229070410977 54-55 0.2510605569698804 56-57 0.2480887011793052 58-59 0.24104469120376426 60-61 0.22460205602481395 62-63 0.22087761013415194 64-65 0.22616440973646698 66-67 0.23192843313868203 68-69 0.22745839798607356 70-71 0.24546105326396342 72-73 0.24911665757957877 74-75 0.25902050993544945 76-77 0.22929495119155666 78-79 0.22594621694775968 80-81 0.21915190353255756 82-83 0.2242753502449313 84-85 0.24430941538987716 86-87 0.2358804062689052 88-89 0.22440369894417092 90-91 0.2262507534068645 92-93 0.21902705525238814 >>END_MODULE >>Sequence Length Distribution pass #Length Count 93 4.2852012E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.49343007014866 #Duplication Level Percentage of deduplicated Percentage of total 1 87.2459739620872 62.37513938360494 2 8.145698304435541 11.647278242013817 3 1.8452503913484357 3.9576983944735153 4 0.8950592658899571 2.55963428138169 5 0.38076326306798536 1.3611035860716316 6 0.30859848044699856 1.3237658328954978 7 0.1721990851422624 0.8617772276233338 8 0.15942384587761962 0.9118206061412606 9 0.10095361172689457 0.6495767982293108 >10 0.6772346031588566 9.131618007125839 >50 0.04457331468403862 2.1736888030898482 >100 0.023630000115696662 2.6674142390780697 >500 5.753908638543993E-4 0.26549117526171145 >1k 5.3599573564806516E-5 0.041352130651025734 >5k 9.080632593222999E-6 0.044146337514064216 >10k+ 3.800948504737879E-6 0.028494954844532617 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 2.3336127134473874E-6 0.0 2 0.0 2.3336127134473874E-6 0.0 3 0.0 2.3336127134473874E-6 2.3336127134473874E-6 4 0.0 4.667225426894775E-6 1.4001676280684324E-5 5 7.000838140342162E-6 4.667225426894775E-6 1.4001676280684324E-5 6 9.33445085378955E-6 7.000838140342162E-6 1.4001676280684324E-5 7 1.633528899413171E-5 7.000838140342162E-6 3.267057798826342E-5 8 2.1002514421026484E-5 7.000838140342162E-6 3.500419070171081E-5 9 2.1002514421026484E-5 9.33445085378955E-6 5.133947969584252E-5 10-11 3.0336965274816034E-5 9.33445085378955E-6 8.634367039755333E-5 12-13 4.7839060625671435E-5 1.1668063567236938E-5 1.0034534667823766E-4 14-15 5.950712419290837E-5 1.4001676280684324E-5 1.2134786109926413E-4 16-17 6.767476868997424E-5 1.983570806430279E-5 1.7268734079510663E-4 18-19 8.401005768410595E-5 2.566973984792126E-5 2.181927887073307E-4 20-21 9.567812125134288E-5 2.566973984792126E-5 2.461961412686994E-4 22-23 1.0851299117530352E-4 2.6836546204644955E-5 3.16204522672121E-4 24-25 1.1668063567236937E-4 3.73378034151582E-5 6.160737563501103E-4 26-27 1.2484828016943523E-4 4.200502884205297E-5 0.0017280402143077904 28-29 1.4351718187701433E-4 4.6672254268947744E-5 0.00821665036404825 30-31 1.5518524544425125E-4 4.900586698239513E-5 0.015059969646232713 32-33 1.6101927722786971E-4 4.900586698239513E-5 0.022982584808386594 34-35 1.8085498529217252E-4 5.367309240928991E-5 0.038309753110309035 36-37 1.9485666157285683E-4 5.600670512273729E-5 0.0745729278709247 38-39 1.995238869997516E-4 5.834031783618468E-5 0.13966905451253958 40-41 2.1235875692371224E-4 6.650796233325054E-5 0.21672494631057232 42-43 2.251936268476729E-4 7.000838140342162E-5 0.30061715655264915 44-45 2.4036210948508087E-4 7.2341994116869E-5 0.4037395023598892 46-47 2.578642048359363E-4 7.2341994116869E-5 0.5322667229720742 48-49 2.7653310654351536E-4 7.2341994116869E-5 0.6628253534513152 50-51 2.952020082510945E-4 7.934283225721116E-5 0.7942404197963914 52-53 3.1387090995867357E-4 8.284325132738225E-5 0.9264535816894666 54-55 3.255389735259105E-4 8.634367039755333E-5 1.0701434509072758 56-57 3.453746815902133E-4 8.867728311100071E-5 1.2346654808180302 58-59 3.6404358329779243E-4 9.101089582444811E-5 1.406093837554232 60-61 3.9438054857260846E-4 9.567812125134287E-5 1.5738875924892395 62-63 4.048818057831217E-4 9.801173396479027E-5 1.7351028931850387 64-65 4.142162566369113E-4 1.0034534667823765E-4 1.8991348644259691 66-67 4.317183519877666E-4 1.0384576574840873E-4 2.0792209243290607 68-69 4.4455322191172726E-4 1.0734618481857982E-4 2.265862102344226 70-71 4.5855489819241163E-4 1.1201341024547458E-4 2.448285275379835 72-73 4.713897681163722E-4 1.2018105474254044E-4 2.6258090285235616 74-75 4.737233808298196E-4 1.2251466745598783E-4 2.8006526274658934 76-77 4.8539144439705654E-4 1.2601508652615892E-4 2.9860406554539374 78-79 4.970595079642935E-4 1.283486992396063E-4 3.1799627051350585 80-81 5.087275715315305E-4 1.3301592466650108E-4 3.3727506190374443 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACTG 18375 0.0 16.51467 5 TACACCG 7960 0.0 15.610607 5 CTATACT 21880 0.0 14.308066 4 GTGTAGG 14950 0.0 14.203625 1 GTATAAG 20240 0.0 14.045866 1 TATACAG 17980 0.0 14.040316 5 TATACCG 6840 0.0 13.895955 5 GTATTAG 19965 0.0 13.780706 1 GTATAGG 14545 0.0 13.669813 1 GTACTAG 13155 0.0 13.390659 1 GTATATA 39030 0.0 13.271785 1 TACACTG 12555 0.0 13.265838 5 TCTATAC 23645 0.0 13.147967 3 GTGTAAG 14075 0.0 12.918114 1 GTATAGA 23415 0.0 12.774426 1 CTTACAC 13630 0.0 12.763959 3 GTATAAT 30790 0.0 12.518549 1 ACTGTGC 11795 0.0 12.428907 8 ACACCGT 6985 0.0 12.271276 6 TTATACT 33950 0.0 12.226467 4 >>END_MODULE