##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename DP18_GGACTCCT-CTCTCTAT_L006_R1_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 42852012 Sequences flagged as poor quality 0 Sequence length 93 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.29294860180661 33.0 33.0 33.0 33.0 33.0 2 32.40928372278062 33.0 33.0 33.0 33.0 33.0 3 32.512246752847915 33.0 33.0 33.0 33.0 33.0 4 36.48394640139651 37.0 37.0 37.0 37.0 37.0 5 36.32095949193704 37.0 37.0 37.0 37.0 37.0 6 36.30586594160386 37.0 37.0 37.0 37.0 37.0 7 36.25670066553701 37.0 37.0 37.0 33.0 37.0 8 36.24193818483949 37.0 37.0 37.0 33.0 37.0 9 36.295918544034755 37.0 37.0 37.0 37.0 37.0 10-11 36.196634186044754 37.0 37.0 37.0 33.0 37.0 12-13 36.08152302160281 37.0 37.0 37.0 33.0 37.0 14-15 37.94461041875933 40.0 37.0 40.0 33.0 40.0 16-17 37.9394209541433 40.0 37.0 40.0 33.0 40.0 18-19 37.97516563516318 40.0 37.0 40.0 33.0 40.0 20-21 37.87618273326349 40.0 37.0 40.0 33.0 40.0 22-23 37.89142264778606 40.0 37.0 40.0 33.0 40.0 24-25 37.84222410606998 40.0 37.0 40.0 33.0 40.0 26-27 37.83155155001823 40.0 37.0 40.0 33.0 40.0 28-29 37.71953384592537 40.0 37.0 40.0 33.0 40.0 30-31 37.74177040975346 40.0 37.0 40.0 33.0 40.0 32-33 37.66506257629163 40.0 37.0 40.0 33.0 40.0 34-35 37.610264390852876 40.0 37.0 40.0 33.0 40.0 36-37 37.50699824783023 40.0 37.0 40.0 33.0 40.0 38-39 37.455667600858504 40.0 37.0 40.0 33.0 40.0 40-41 37.3738599601811 40.0 37.0 40.0 33.0 40.0 42-43 37.31952444846697 40.0 37.0 40.0 33.0 40.0 44-45 37.23763503800008 40.0 37.0 40.0 33.0 40.0 46-47 37.108462118418146 40.0 37.0 40.0 33.0 40.0 48-49 36.96207692651631 38.5 37.0 40.0 33.0 40.0 50-51 36.82392090481072 37.0 37.0 40.0 33.0 40.0 52-53 36.730533947857566 37.0 37.0 40.0 33.0 40.0 54-55 36.57986721837005 37.0 37.0 40.0 33.0 40.0 56-57 36.437413335457855 37.0 37.0 40.0 33.0 40.0 58-59 36.25696067666554 37.0 37.0 40.0 33.0 40.0 60-61 35.99901173835198 37.0 37.0 40.0 30.0 40.0 62-63 35.776507856853954 37.0 35.0 40.0 27.0 40.0 64-65 35.61340166478064 37.0 33.0 40.0 27.0 40.0 66-67 35.43436987276117 37.0 33.0 40.0 27.0 40.0 68-69 35.20474722400432 37.0 33.0 40.0 27.0 40.0 70-71 35.02696078774551 37.0 33.0 37.0 27.0 40.0 72-73 34.740186668481286 37.0 33.0 37.0 27.0 40.0 74-75 34.429869232277824 37.0 33.0 37.0 27.0 40.0 76-77 32.925637470651324 35.0 33.0 37.0 27.0 37.0 78-79 33.965395603828355 37.0 33.0 37.0 27.0 37.0 80-81 34.03960238786454 37.0 33.0 37.0 27.0 37.0 82-83 33.89166879725508 37.0 33.0 37.0 27.0 37.0 84-85 33.760350634177925 37.0 33.0 37.0 27.0 37.0 86-87 33.605421724422186 37.0 33.0 37.0 27.0 37.0 88-89 33.43899109101341 37.0 33.0 37.0 27.0 37.0 90-91 33.29990109915959 37.0 33.0 37.0 27.0 37.0 92-93 32.095725166883646 35.0 30.0 37.0 24.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2304 1 0.28670037810002924 2304 2 0.27976984455099085 2304 3 0.2253543821557642 2304 4 0.2077089426550458 2304 5 0.16092090402398185 2304 6 0.22901074730759063 2304 7 0.24253722296258218 2304 8 0.26749182936767824 2304 9 0.22721333828282297 2304 10-11 0.27642298802810217 2304 12-13 0.3523512987792472 2304 14-15 0.6522277911507999 2304 16-17 0.6349997473080435 2304 18-19 0.5550169772216123 2304 20-21 0.6381702087159553 2304 22-23 0.6255354799680148 2304 24-25 0.6725824021674498 2304 26-27 0.652943341872728 2304 28-29 0.45206231348790027 2304 30-31 0.4983430823107682 2304 32-33 0.5588180592033964 2304 34-35 0.6034822850306725 2304 36-37 0.6051943949597032 2304 38-39 0.5954764886685169 2304 40-41 0.5404119957205822 2304 42-43 0.5743281302209269 2304 44-45 0.5819800631130434 2304 46-47 0.6434872904451368 2304 48-49 0.5744761061146946 2304 50-51 0.6063001301758533 2304 52-53 0.7062611860808374 2304 54-55 0.6645968846078674 2304 56-57 0.6481365399558641 2304 58-59 0.5517491415322482 2304 60-61 0.5997850839773449 2304 62-63 0.44273928713481325 2304 64-65 0.5081532035152065 2304 66-67 0.4132634622074747 2304 68-69 0.4492436209837436 2304 70-71 0.5238147922079506 2304 72-73 0.5189339088324587 2304 74-75 0.583701311830886 2304 76-77 0.445677180643429 2304 78-79 0.4960921058223917 2304 80-81 0.652201329726779 2304 82-83 0.728516218404998 2304 84-85 0.673223329202024 2304 86-87 0.5810112863450598 2304 88-89 0.527002505766518 2304 90-91 0.7302430410262559 2304 92-93 0.8402650071771234 2305 1 0.31783412093868435 2305 2 0.3162936289848943 2305 3 0.21834432682141625 2305 4 0.19621945040163524 2305 5 0.19731085446983343 2305 6 0.27542312939695535 2305 7 0.31502855821227627 2305 8 0.2520048804046695 2305 9 0.20951990704169532 2305 10-11 0.2674267651700575 2305 12-13 0.338608082183832 2305 14-15 0.6677971080416754 2305 16-17 0.6395494359550611 2305 18-19 0.5336132098615423 2305 20-21 0.6560510520034768 2305 22-23 0.6198563169417639 2305 24-25 0.6313647574233272 2305 26-27 0.5027899441123367 2305 28-29 0.5606150167343742 2305 30-31 0.581286029459676 2305 32-33 0.5527706303944058 2305 34-35 0.5058310492894336 2305 36-37 0.4227603282072252 2305 38-39 0.4400111932444375 2305 40-41 0.4542016762753036 2305 42-43 0.37450453764538594 2305 44-45 0.48228530242467116 2305 46-47 0.4625567440601088 2305 48-49 0.38259584974035477 2305 50-51 0.4459004289680095 2305 52-53 0.5234496274484357 2305 54-55 0.4253666301232073 2305 56-57 0.39873445774799876 2305 58-59 0.4696764487790617 2305 60-61 0.4505003844035187 2305 62-63 0.4247351698499955 2305 64-65 0.44204988456569794 2305 66-67 0.30712277200647975 2305 68-69 0.3248407050923987 2305 70-71 0.38697509731387214 2305 72-73 0.2876649981121773 2305 74-75 0.32194137265643263 2305 76-77 0.2569775934834837 2305 78-79 0.4956301340164586 2305 80-81 0.537598974515376 2305 82-83 0.4896015409071097 2305 84-85 0.45065051528685274 2305 86-87 0.46352972186576125 2305 88-89 0.5545354029166489 2305 90-91 0.46709418517346535 2305 92-93 0.5883743242013821 2306 1 0.335935893380082 2306 2 0.20737805818253463 2306 3 0.1846641286129156 2306 4 0.14580206904085458 2306 5 0.14601296996440283 2306 6 0.014230066694459254 2306 7 0.06042211643602258 2306 8 0.24071461930308402 2306 9 0.13998302882557567 2306 10-11 0.23256875106675778 2306 12-13 0.3804396049587524 2306 14-15 0.3679667287773327 2306 16-17 0.30230910814869105 2306 18-19 0.06054478981199196 2306 20-21 0.3125633447421805 2306 22-23 0.3675426138733471 2306 24-25 0.2542521216314526 2306 26-27 0.2869122997441238 2306 28-29 0.361087667773063 2306 30-31 0.4244859532256413 2306 32-33 0.5034319606783839 2306 34-35 0.40172965801823324 2306 36-37 0.20318533297185581 2306 38-39 0.4323954127984706 2306 40-41 0.5799897456482768 2306 42-43 0.4459911013960962 2306 44-45 0.16299809068463134 2306 46-47 0.011874449415543609 2306 48-49 0.09504296237614085 2306 50-51 0.16254422542312597 2306 52-53 0.37514964650694793 2306 54-55 0.23862754111996054 2306 56-57 0.4309014102923143 2306 58-59 0.4465356063929349 2306 60-61 0.3272756845750493 2306 62-63 0.22310947897902622 2306 64-65 0.420647178257326 2306 66-67 0.2952436029574983 2306 68-69 0.3473907336801645 2306 70-71 0.48880852593975277 2306 72-73 0.12888474272814676 2306 74-75 0.363793859791933 2306 76-77 0.21845843968131362 2306 78-79 0.44693564192574087 2306 80-81 0.7857788868462308 2306 82-83 0.3571887335886501 2306 84-85 0.49009019510388896 2306 86-87 0.2957919669581912 2306 88-89 0.40576467678742745 2306 90-91 0.49715136070233257 2306 92-93 0.43447304728124436 2307 1 0.4965852440294327 2307 2 0.4298455906500678 2307 3 0.2738849078336969 2307 4 0.13117003440882513 2307 5 0.3509047448561802 2307 6 0.3315027939671893 2307 7 0.507002203016107 2307 8 0.4661258747143435 2307 9 0.3032081370506816 2307 10-11 0.4179583614563711 2307 12-13 0.5445305140496615 2307 14-15 0.8151204084310066 2307 16-17 0.7378610562006429 2307 18-19 0.9333586426258407 2307 20-21 0.9785914832703213 2307 22-23 0.9986898000205287 2307 24-25 0.8732672731465954 2307 26-27 0.8191309144627326 2307 28-29 0.7466287933141871 2307 30-31 0.8452759965156815 2307 32-33 0.870163562409985 2307 34-35 1.0351062813948602 2307 36-37 0.9328606576471756 2307 38-39 0.772243897646959 2307 40-41 0.5772624729210065 2307 42-43 0.6665755169805081 2307 44-45 0.4749028525893877 2307 46-47 0.6834761810172765 2307 48-49 0.6320343043674868 2307 50-51 0.4488429267218308 2307 52-53 0.49599380235110146 2307 54-55 0.7557162857087079 2307 56-57 0.6970161289070305 2307 58-59 0.6527477276050604 2307 60-61 1.133249710549073 2307 62-63 0.6804254962950438 2307 64-65 0.8705389531491079 2307 66-67 0.8169535596674606 2307 68-69 0.5687760150654455 2307 70-71 0.8985054956367193 2307 72-73 0.5934301972736051 2307 74-75 0.5300276260256993 2307 76-77 0.6655580067808771 2307 78-79 1.1019681094582054 2307 80-81 0.888538627105973 2307 82-83 1.1571779110778238 2307 84-85 0.658455995969689 2307 86-87 1.00470971587594 2307 88-89 0.9020309105536626 2307 90-91 0.7758093693603456 2307 92-93 0.50886698667518 2308 1 0.22202925947344454 2308 2 0.19528960609407875 2308 3 0.16264938659817574 2308 4 0.11037209361088429 2308 5 0.1651775891290228 2308 6 0.14907036153475417 2308 7 0.116834892848793 2308 8 0.11275393134239664 2308 9 0.10130981015235818 2308 10-11 0.1897923382903457 2308 12-13 0.16493591945506836 2308 14-15 0.3722664830770839 2308 16-17 0.30619117203075064 2308 18-19 0.2544268536940564 2308 20-21 0.3207697329485626 2308 22-23 0.2298030561337825 2308 24-25 0.18675826413759467 2308 26-27 0.10349706632889166 2308 28-29 0.1579672746526697 2308 30-31 0.017016665756358407 2308 32-33 0.1470683243147448 2308 34-35 -0.019719973431392646 2308 36-37 -0.012023512236986278 2308 38-39 -0.07401736361430267 2308 40-41 -0.06652131086277535 2308 42-43 -0.07884919344419927 2308 44-45 -0.08538028769375217 2308 46-47 0.004184031725131376 2308 48-49 -0.08407251673934013 2308 50-51 0.007163390042293827 2308 52-53 -0.004064112706814171 2308 54-55 0.06956120205362737 2308 56-57 0.09323556262646804 2308 58-59 0.15918916904650615 2308 60-61 0.05290222020158808 2308 62-63 -0.14539174952220435 2308 64-65 -0.07937739176723824 2308 66-67 -0.1278276501137512 2308 68-69 -0.2098082835188535 2308 70-71 -0.1240170268858023 2308 72-73 -0.07467791083450237 2308 74-75 -0.03333144733337434 2308 76-77 -0.13009192886905652 2308 78-79 0.056591661581762764 2308 80-81 0.026810827878165355 2308 82-83 -0.0033272369273262825 2308 84-85 -0.015118650104959386 2308 86-87 -0.07418346301723489 2308 88-89 -0.06683974581699204 2308 90-91 -0.05385601030503295 2308 92-93 0.0947678875760829 2309 1 0.314163829046187 2309 2 0.28547110020709 2309 3 0.15084387150793788 2309 4 0.12801455534972916 2309 5 0.1645729515174068 2309 6 0.1426949309361163 2309 7 0.1494818962250335 2309 8 0.17095270081573943 2309 9 0.1262031245193569 2309 10-11 0.1556044344702343 2309 12-13 0.2655989985138305 2309 14-15 0.20726228420478066 2309 16-17 0.3041883956335596 2309 18-19 0.19776967019476643 2309 20-21 0.21462018454157317 2309 22-23 0.2683058582383637 2309 24-25 0.2589019169851099 2309 26-27 0.22941302032981525 2309 28-29 0.12201001745889073 2309 30-31 0.1427761474955389 2309 32-33 0.06826046128367125 2309 34-35 0.20715538004510137 2309 36-37 0.07622735752941168 2309 38-39 -0.02909302900702926 2309 40-41 0.07456748243274092 2309 42-43 -0.02740718072761439 2309 44-45 0.05653011351278536 2309 46-47 0.0016813324500759563 2309 48-49 -0.03215251811039366 2309 50-51 0.06172679301406703 2309 52-53 0.04863670478791704 2309 54-55 0.1462841964494217 2309 56-57 0.08838800739844288 2309 58-59 0.08207028097644553 2309 60-61 0.07799108205011152 2309 62-63 0.1054338001872992 2309 64-65 -0.025130189587258656 2309 66-67 -0.053826553265473365 2309 68-69 -0.09728673216407202 2309 70-71 -0.18625459736686878 2309 72-73 -0.056696625808193346 2309 74-75 -0.004667545638397996 2309 76-77 -0.31475421956812966 2309 78-79 0.09070689920365282 2309 80-81 0.2990434282654917 2309 82-83 0.20513477613202724 2309 84-85 0.16833196601528755 2309 86-87 0.2967500576943394 2309 88-89 0.32222861867940367 2309 90-91 0.14002563291079184 2309 92-93 0.29028212089627914 2310 1 0.26429952974371673 2310 2 0.18255987636435123 2310 3 0.15002776497654935 2310 4 0.08707479631358694 2310 5 0.1532856972371306 2310 6 0.16950279396719026 2310 7 0.13424029825419836 2310 8 0.06107825566672176 2310 9 -0.052744243901699406 2310 10-11 0.0658869328849434 2310 12-13 0.16753051404966612 2310 14-15 0.11323945605006003 2310 16-17 0.18221819905778602 2310 18-19 0.008453880721084772 2310 20-21 0.07847243565127116 2310 22-23 0.013451704782433183 2310 24-25 -0.04056606018673392 2310 26-27 -0.18067860934678492 2310 28-29 -0.12627596859057633 2310 30-31 -0.13860495586526866 2310 32-33 0.004068324314744132 2310 34-35 -0.00617943289085332 2310 36-37 -0.09749648520996601 2310 38-39 -0.2174227690197057 2310 40-41 -0.23873752707899598 2310 42-43 -0.25278162587662933 2310 44-45 -0.22600190931537156 2310 46-47 -0.17826191422081905 2310 48-49 -0.2092752194420342 2310 50-51 -0.2337285018495976 2310 52-53 -0.034577626220333 2310 54-55 -0.12923609524366952 2310 56-57 -0.08500768061677633 2310 58-59 -0.18841893906159868 2310 60-61 -0.04667886087950279 2310 62-63 -0.23352688465733706 2310 64-65 -0.32908009446994413 2310 66-67 -0.20616548795158707 2310 68-69 -0.3082001754107395 2310 70-71 -0.3333278376966078 2310 72-73 -0.3610698027263908 2310 74-75 -0.2021152311171548 2310 76-77 -0.3041324694095948 2310 78-79 -0.3757461762560794 2310 80-81 0.03732434139168106 2310 82-83 -0.08158399368408453 2310 84-85 -0.11809162307793031 2310 86-87 -0.32898076031453627 2310 88-89 -0.44482623230347684 2310 90-91 -0.31116682111584737 2310 92-93 -0.6112996799914825 2311 1 0.1823951320381738 2311 2 0.12425968515976393 2311 3 0.01773904604729637 2311 4 -0.18245101630591876 2311 5 -0.11019422628103825 2311 6 -0.023659729933385165 2311 7 -0.12433713960430737 2311 8 -0.023380635346661904 2311 9 -0.08371938730513762 2311 10-11 -0.19058438642672115 2311 12-13 -0.029322258416875968 2311 14-15 -0.10009515198053265 2311 16-17 -0.21930570151583595 2311 18-19 -0.18084057434584366 2311 20-21 -0.031384160907052205 2311 22-23 -0.23541062791355216 2311 24-25 -0.2412563087526962 2311 26-27 -0.2473411332473603 2311 28-29 -0.28296239306476423 2311 30-31 -0.171756007490508 2311 32-33 -0.45038865465275535 2311 34-35 -0.3264069663516622 2311 36-37 -0.42773071083137637 2311 38-39 -0.38557955678261635 2311 40-41 -0.5427184066201036 2311 42-43 -0.483690803696895 2311 44-45 -0.45334990166718825 2311 46-47 -0.5981863884081946 2311 48-49 -0.386278087510874 2311 50-51 -0.33608605443085793 2311 52-53 -0.5021282954363855 2311 54-55 -0.3215793074807678 2311 56-57 -0.473047833580452 2311 58-59 -0.42237974212087437 2311 60-61 -0.6159991285659245 2311 62-63 -0.7507180892462486 2311 64-65 -0.6333420447567448 2311 66-67 -0.6128356600357208 2311 68-69 -0.6159640377434457 2311 70-71 -0.6958221015589388 2311 72-73 -0.6178508734720936 2311 74-75 -0.7764460150559671 2311 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34-35 -0.7017472445884536 2303 36-37 -0.5904806920066576 2303 38-39 -0.6189510069435187 2303 40-41 -0.6398607188764132 2303 42-43 -0.7448748912925538 2303 44-45 -0.7770166067708715 2303 46-47 -0.7531890546471374 2303 48-49 -0.6083226515428564 2303 50-51 -0.5403374620483632 2303 52-53 -0.6049091194555629 2303 54-55 -0.8775071473802711 2303 56-57 -0.8922473366263333 2303 58-59 -0.9073902724620098 2303 60-61 -1.048136386982783 2303 62-63 -1.1432461763799608 2303 64-65 -1.3027351373156506 2303 66-67 -1.362747386897837 2303 68-69 -1.2297067892657196 2303 70-71 -1.254967725978453 2303 72-73 -1.6619173144384263 2303 74-75 -1.083165902344696 2303 76-77 -0.9493572026901447 2303 78-79 -1.3048670431435028 2303 80-81 -1.1277996771376095 2303 82-83 -1.0157324532945111 2303 84-85 -0.9050368269395079 2303 86-87 -0.8834119447282376 2303 88-89 -0.8561124061971199 2303 90-91 -0.6642797006360794 2303 92-93 -0.8071456213503865 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 31.0 13 146.0 14 565.0 15 1860.0 16 4685.0 17 10573.0 18 20195.0 19 34321.0 20 53337.0 21 78410.0 22 110501.0 23 149956.0 24 197862.0 25 257001.0 26 326844.0 27 411830.0 28 518585.0 29 651877.0 30 821803.0 31 1045941.0 32 1352374.0 33 1794356.0 34 2468063.0 35 3651517.0 36 6023265.0 37 1.0989244E7 38 1.161799E7 39 258879.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.94676177081021 24.75419545432865 11.816898346876984 24.482144427984156 2 15.382682614762638 23.194192142016576 38.533768262736416 22.889356980484372 3 15.118802356351434 27.74783597092244 32.10043906456481 25.032922608161318 4 11.3458173212497 17.76879461342445 42.16584742858748 28.719540636738362 5 11.371447389681492 38.88272037261634 38.53223041195825 11.213601825743911 6 28.12803748864814 42.66207150320036 18.30758378393061 10.902307224220884 7 25.538705160448476 33.06928972203219 26.585552155637405 14.80645296188193 8 22.859927790555084 38.265227779736456 22.986939329709884 15.887905099998573 9 23.407631361626613 15.090908683587598 25.200520339628397 36.3009396151574 10-11 22.19803938260822 27.42863858994532 31.999174274477475 18.374147752968987 12-13 21.761590797650296 25.664588397856324 31.952629202101406 20.62119160239197 14-15 19.621907134722168 27.333554373129555 27.501260617587803 25.543277874560477 16-17 19.734894828275507 31.931753869573264 28.627759648718477 19.705591653432748 18-19 19.80661258099153 30.60809140070249 30.21475981104458 19.3705362072614 20-21 20.142966682637912 30.15577658290584 29.99277140125883 19.708485333197423 22-23 20.08373842516426 29.957638861857877 30.04705473339268 19.911567979585183 24-25 19.776542066059154 29.81033212064662 30.301251345387765 20.11187446790646 26-27 19.99176999119475 30.384482152360015 29.99902500987358 19.62472284657165 28-29 19.97360767110952 30.22952302019291 30.05052839556233 19.746340913135235 30-31 19.793225292938846 30.106959423472063 30.1890047850732 19.910810498515882 32-33 19.973605124452128 30.328574743811988 30.05278753415676 19.645032597579124 34-35 19.84480211430052 30.014174664462235 30.213277115401993 19.927746105835254 36-37 19.793023610148577 30.25939596177587 30.212202594918747 19.735377833156807 38-39 19.85254686793362 30.249655632978456 30.2161515131117 19.681645985976225 40-41 19.689300848499595 30.150962492697676 30.373525368533237 19.786211290269488 42-43 19.708912964541177 30.165815052567023 30.385748983243705 19.73952299964809 44-45 19.69264699741151 30.04540447387381 30.337822526594515 19.924126002120165 46-47 19.622955156762202 30.00693271816622 30.514349207417485 19.85576291765409 48-49 19.694769817032316 30.250845724735402 30.344127564228167 19.710256894004115 50-51 19.603847640719593 30.172639630959843 30.469642645200373 19.75387008312019 52-53 19.685844082090913 30.05267498821115 30.571546576621735 19.689934353076204 54-55 19.611882976287337 30.15907586290042 30.496747053577618 19.732294107234626 56-57 19.597541789910206 30.16017465614921 30.394541271262536 19.847742282678045 58-59 19.61088807269168 30.19637816359039 30.364946549716805 19.82778721400113 60-61 19.507896164515838 30.118436189022567 30.492369585745806 19.881298060715793 62-63 19.487596338269604 30.224961255350024 30.38566171787465 19.901780688505724 64-65 19.509260220892948 30.10407373082818 30.47005494110732 19.91661110717155 66-67 19.495788698860455 30.047090125781217 30.413034490742664 20.044086684615664 68-69 19.458372349658905 30.222138950012955 30.331437423872682 19.988051276455458 70-71 19.554729833370303 30.12733661097734 30.302122589910297 20.015810965742062 72-73 19.55347220774263 30.13110711892984 30.22184757316126 20.093573100166275 74-75 19.476531200452154 30.175278913507235 30.275555741004613 20.072634145035995 76-77 19.48754688484993 30.1092233145797 30.280452655015534 20.12277714555483 78-79 19.54855148460924 30.07496113306803 30.237489736563006 20.138997645759723 80-81 19.453537687856112 30.188202521609526 30.141512839131362 20.216746951403003 82-83 19.485367176434213 30.15258281641077 30.198700170336846 20.163349836818167 84-85 19.40553673849121 30.130835420033215 30.248160162835486 20.21546767864009 86-87 19.295633458354285 30.03858090121566 30.291611608362107 20.374174032067945 88-89 19.402396826910756 30.06685414131075 30.19763119007406 20.333117841704432 90-91 19.483380750491463 30.11754545003919 30.151795583553103 20.24727821591624 92-93 19.46641412125687 30.001147109828725 30.13762529222801 20.394813476686398 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3.0 1 3.5 2 7.5 3 25.5 4 75.0 5 195.0 6 445.5 7 917.5 8 1783.5 9 2989.0 10 4511.0 11 6535.5 12 9391.0 13 13831.0 14 16564.0 15 20461.5 16 30485.5 17 45270.0 18 67094.5 19 99369.5 20 145372.5 21 207807.0 22 293033.5 23 400564.5 24 524951.5 25 670730.5 26 838001.0 27 1017960.0 28 1199628.5 29 1289372.0 30 1370385.0 31 1523790.0 32 1656642.5 33 1762740.0 34 1848729.5 35 1911580.0 36 1937834.5 37 1929834.0 38 1890415.5 39 1829994.0 40 1754365.0 41 1664072.0 42 1569757.5 43 1522867.0 44 1469003.0 45 1351460.0 46 1234843.5 47 1128016.0 48 1024237.5 49 925949.5 50 834859.0 51 754877.0 52 679620.5 53 608823.5 54 546212.0 55 488545.5 56 437369.5 57 414080.0 58 394164.0 59 355642.5 60 319576.5 61 286727.5 62 255757.5 63 225883.0 64 200324.5 65 176262.5 66 153544.0 67 133129.0 68 113642.0 69 94080.0 70 75905.0 71 68298.0 72 62357.0 73 50537.0 74 40295.5 75 34276.0 76 30884.5 77 26266.0 78 21262.0 79 16676.0 80 13673.5 81 11548.0 82 8909.5 83 6210.0 84 3857.5 85 2517.0 86 1879.0 87 1422.5 88 673.5 89 297.0 90 153.5 91 75.0 92 46.0 93 23.5 94 8.0 95 4.5 96 2.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09464666443199914 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.2601508652615892E-4 26-27 6.907493631804266E-4 28-29 3.8854651678898994E-4 30-31 2.508633666955941E-4 32-33 3.115372972452262E-4 34-35 4.76056993543267E-4 36-37 0.0015191818764542491 38-39 0.0013196579894544974 40-41 1.7852137257872512E-4 42-43 1.2134786109926413E-4 44-45 4.1421625663691123E-4 46-47 3.103704908885025E-4 48-49 3.628767769410687E-4 50-51 2.1002514421026485E-4 52-53 0.015028465874601174 54-55 0.06316506212123715 56-57 0.09042049180794591 58-59 0.10236742209443982 60-61 0.11357109673170071 62-63 0.11963032214216687 64-65 0.12262434725351987 66-67 0.11926394494615562 68-69 0.1165651218430537 70-71 0.11389780251158335 72-73 0.11086877320952865 74-75 0.1143528569907056 76-77 0.11000650331190982 78-79 0.09013579105690533 80-81 0.0798118884126141 82-83 0.05927492972792036 84-85 0.038811479843700224 86-87 0.020519456589342875 88-89 0.0153400031718464 90-91 0.005936710743010153 92-93 0.0019614014856525292 >>END_MODULE >>Sequence Length Distribution pass #Length Count 93 4.2852012E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.44291520003132 #Duplication Level Percentage of deduplicated Percentage of total 1 87.36012570448436 62.41262052569553 2 8.186408890029032 11.697218322462533 3 1.7883048261248518 3.8328513013393364 4 0.7471385340440171 2.135110197215297 5 0.4124939732189639 1.4734885974603216 6 0.25928951026515423 1.1114639096478638 7 0.17981306044534537 0.899245846047844 8 0.14642396946600483 0.8368764187049408 9 0.11851007384306174 0.7620034640327379 >10 0.7271512756607306 9.61901030699744 >50 0.05485390268790706 2.6255941640526745 >100 0.018987224378196953 2.2503464250844822 >500 3.9802982639707354E-4 0.18601000826473166 >1k 8.818045239758119E-5 0.08526744122264146 >5k 9.048901689451187E-6 0.04433199017385762 >10k+ 3.796171896847102E-6 0.028561081597617374 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 2.3336127134473874E-6 0.0 0.0 2 2.3336127134473874E-6 2.3336127134473874E-6 2.3336127134473874E-6 3 2.3336127134473874E-6 2.3336127134473874E-6 9.33445085378955E-6 4 2.3336127134473874E-6 9.33445085378955E-6 1.86689017075791E-5 5 9.33445085378955E-6 1.1668063567236936E-5 1.86689017075791E-5 6 2.3336127134473872E-5 1.633528899413171E-5 1.86689017075791E-5 7 2.3336127134473872E-5 1.86689017075791E-5 2.1002514421026484E-5 8 3.73378034151582E-5 1.86689017075791E-5 2.3336127134473872E-5 9 3.9671416128605586E-5 1.86689017075791E-5 2.566973984792126E-5 10-11 4.900586698239513E-5 2.5669739847921257E-5 6.184073690635576E-5 12-13 6.067393054963207E-5 3.61709970584345E-5 8.284325132738225E-5 14-15 6.767476868997424E-5 3.9671416128605586E-5 1.1084660388875088E-4 16-17 7.817602590048748E-5 4.4338641555500356E-5 1.5635205180097492E-4 18-19 9.801173396479027E-5 4.7839060625671435E-5 2.0769153149681745E-4 20-21 1.1318021660219828E-4 5.367309240928991E-5 2.4619614126869933E-4 22-23 1.3418273102322475E-4 5.950712419290838E-5 3.2203855445573943E-4 24-25 1.470176009471854E-4 6.300754326307946E-5 6.230745944904524E-4 26-27 1.5635205180097492E-4 6.534115597652684E-5 0.001740875084231751 28-29 1.7502095350855406E-4 6.534115597652684E-5 0.008246987329323066 30-31 1.9252304885940947E-4 6.767476868997422E-5 0.015199986409039556 32-33 1.9719027428630423E-4 6.884157504669792E-5 0.023283620848421303 34-35 2.0769153149681745E-4 7.2341994116869E-5 0.038851151259828826 36-37 2.205264014207781E-4 7.2341994116869E-5 0.07561371914112223 38-39 2.3219446498801502E-4 7.35088004735927E-5 0.14133292037722756 40-41 2.4269572219852827E-4 7.58424131870401E-5 0.21928958668265097 42-43 2.520301730523178E-4 7.700921954376378E-5 0.30385621099891413 44-45 2.6603184933300216E-4 7.700921954376378E-5 0.407697309521896 46-47 2.823671383271339E-4 8.050963861393486E-5 0.5373609995255298 48-49 2.9286839553764707E-4 8.517686404082963E-5 0.6690327632690852 50-51 3.0220284639143665E-4 9.334450853789549E-5 0.8014956217224993 52-53 3.127041036019499E-4 9.451131489461919E-5 0.9343330250164217 54-55 3.2203855445573943E-4 9.801173396479027E-5 1.0789493384814697 56-57 3.3487342437970007E-4 1.0151215303496134E-4 1.2442671303275095 58-59 3.477082943036607E-4 1.0617937846185612E-4 1.4161388734792664 60-61 3.8154567864864786E-4 1.0734618481857982E-4 1.5840819796279344 62-63 4.025481930696743E-4 1.0734618481857982E-4 1.7457359995138617 64-65 4.212170947772534E-4 1.1318021660219828E-4 1.9102977008407447 66-67 4.247175138474245E-4 1.1784744202909306E-4 2.091025504240034 68-69 4.3521877105793773E-4 1.2018105474254044E-4 2.278514950476538 70-71 4.422196091982799E-4 1.2484828016943523E-4 2.4620115853603326 72-73 4.503872536953458E-4 1.306823119530537E-4 2.640440780236877 74-75 4.620553172625827E-4 1.3301592466650108E-4 2.8164465183105056 76-77 4.725565744730959E-4 1.3534953737994845E-4 3.003045691296829 78-79 4.830578316836092E-4 1.3768315009339584E-4 3.1986817795159768 80-81 4.8655825075378026E-4 1.44683988233738E-4 3.3931043891241326 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACTG 18790 0.0 17.678095 5 CTATACT 21605 0.0 14.066686 4 GTATTAG 19375 0.0 13.814454 1 TACACTG 13015 0.0 13.4626255 5 ATACTGT 20100 0.0 13.432734 6 TTATACT 34440 0.0 13.318589 4 TATACAG 18540 0.0 13.249748 5 TATACCG 6770 0.0 13.1654 5 GTGTAGG 14480 0.0 13.104433 1 TACACCG 7870 0.0 13.037856 5 TCTATAC 22830 0.0 12.892931 3 CTTACAC 13845 0.0 12.843968 3 GTATAGG 14310 0.0 12.5910225 1 TAGACTG 12355 0.0 12.527839 5 ATTATAC 28710 0.0 12.463372 3 GTATAAG 19690 0.0 12.377776 1 GTATAGA 24015 0.0 12.268923 1 ACACCGT 6790 0.0 12.102105 6 CCTATAC 14905 0.0 12.018054 3 ACTGTGC 12780 0.0 11.975143 8 >>END_MODULE