FastQCFastQC Report
Tue 20 Sep 2016
Undetermined_S0_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUndetermined_S0_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16229033
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTTT150.002225431670.0000170
GTGTCGG5430500.069.78286
TGTCGGT5432600.069.757767
TCGGTCT5435250.069.728269
TTCGTGT123800.069.689013
ACCGGTG132450.069.68292
GTCGGTC5438600.069.682738
CGGTCTC5440900.069.6249710
GGTCTCG5439200.069.5791611
GTCTCGT5434500.069.4995912
CTCGTAG5318250.069.456414
ACGAGGT7870800.069.422810
CGAGGTC7871750.069.4037511
GGTCTCT7835550.069.3983214
CCACGAG7877300.069.392628
GAGGTCT7875650.069.3884912
CGTCCAC3307800.069.387365
AGGTCTC7874450.069.347513
CACGAGG7880500.069.333369
TCCACGA7887050.069.324147
Produced by FastQC (version 0.11.2)