FastQCFastQC Report
Thu 21 Aug 2014
Complete_Undetermined_L001_R3_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameComplete_Undetermined_L001_R3_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14750073
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGCCTAA244930116.60534832607269No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGT9105916.173467751651128No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGATCAG5953264.0360884993586135No Hit
CAAGCAGAAGACGGGCGGTGTGTACCGAACGGCGATAGTGTAGATCTCGG5228673.5448434729780662No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAGTGGA4864463.2979226611285246No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAATGGA3917672.6560343125081483No Hit
GGTAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAATGAA2974712.0167425612063075No Hit
AGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGCATAA2351971.5945480405419008No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACCAG1842511.2491531397844609No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCGAGTTTGACGAA1799011.2196617603180675No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAACGACCAGGTTTGATCAA1725391.169750142931496No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATAACCAAGTTCGGTCAA1612131.092964082279457No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACTAG1530311.0374931703727839No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTCGTACAA1500241.0171068305899231No Hit
TGCTACCTTGTTACGACTTCACCCCAGTCATGAAACATACCGTGGTAATC1438900.9755205957285771No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACCAA1349850.9151480131657653No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTACTTAA1142430.7745249803170466No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACGGAGTTTGACCAA920280.6239155562145353No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGAAATTTGAGTTCGACAGA880300.5968106056153077No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGATCAA859940.583007284099543No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCACAAAGTGGTAAGC830070.5627565368659531No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCGAGTTTGACCAA666370.45177403528782534No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGAAATTTGAGTTCGACAAA641890.43517750725708276No Hit
AAGCAGAAGACGGGCGGTGTGTACCGAACGGCGATAGTGTAGATCTCGGT628740.4262622971425294No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAGTGGA569820.3863167321273597No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCGAGTTTGACCAG568710.38556419347890686No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATAAGGTTTACGAAA526440.35690670819052894No Hit
GGTAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAGTGAA512990.3477881092520695No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATCGGCCACACCGTGGTAAGC511400.34671014848536685No Hit
GGAGACCTTGTTACGACTTTTACTTCCTCTAAATGACCGAGTTTGACGAA508970.3450626990117269No Hit
AGAAGACGGGCGGTGTGTACCGAACGGCGATAGTGTAGATCTCGGTGGTC501930.3402898412773957No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGCCTAA480890.3260255050941104No Hit
GGAAACCTTGTTACGACTTTTACGACCTCTAAATGACCAAGTTTGACTAA433320.293774817250057No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAATCACACCGTGGTAACC428630.29059517196965734No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGAGCTT409720.27777489643610576No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAGGTGGGAGGGTTTAACGAA406930.27588338037377846No Hit
GGAAACCTTGTTACGACTTTTGCTTCCTCTAAATATAAACATTCAATAGT402640.27297492019192043No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGGACAA380860.2582088915763332No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGCCAAGTTCGACCGT378220.256419069925959No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCGGGTTTGACCAA375470.25455467237348584No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACTAA371380.25178180474089856No Hit
CGTTACCTTGTTACGACTTCTCCTTCCTCTAGATAACGTGATTTAATCAC366020.24814792442044184No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAGTGATATGGTTTACTAGA336840.22836497148183607No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTTGATCGT333100.22582939081047262No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTTGATCGA329350.2232870305116456No Hit
GGTTACCTTGTTACGACTTCACCCCAGTCATGAATCATACCGTGGTAAAC327430.22198534203864617No Hit
GGAGACCTTGTTACGACTTTTACTTCCTCTAAATGACCGGGTTTGACCAA317580.21530740898706063No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGATCGA291590.19768715720932364No Hit
AGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGT274140.1858567072854487No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAATGAA271510.18407366526253804No Hit
GAATACCTTGTTACGACTTTTACTTCCTCTAAATGATCCAGTTTGAGCAT262640.17806013570237925No Hit
GACGGGCGGTGTGTACCGAACGGCGATAGTGTAGATCTCGGTGGTCGCCG261340.1771787841321192Illumina Single End PCR Primer 1 (100% over 23bp)
AGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACTAA260300.1764737028759112No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGGTCAT249510.16915848484275298No Hit
GGAAACCTTGTTACGACTTTTACGACCTCTAAATGACCAAGTTTGACTAG249240.16897543490123745No Hit
GGAAACCTTGTTACGACTTCACCTTCCTCTAAATGATGAGATTTAAATAA247440.16775510195780047No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAACGACCAAGTTTGACGAA246250.16694832628963938No Hit
CAGAAGACGGGCGGTGTGTACCGAACGGCGATAGTGTAGATCTCGGTGGT237230.1608331023175275No Hit
AGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGATCAG221340.1500602742779646No Hit
TGAAACTTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTTGACTGT206150.13976202016084938No Hit
AGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGCCCAA205740.1394840554348443No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAGGTTTGACCAA205010.13898914263000595No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGACGAA201200.13640610456639773No Hit
GAAAACCTTGTTACGACTTTTACTTCCTCTAAATGATCAAGTTTGGTCAT192810.1307179971244888No Hit
CAAGCAGAAGACGGGCGGTGTGTACCGACCGGCGATAGTGTAGATCTCGG187270.1269620835096884No Hit
CAAGCAGAAGACGGGCGGTGTGCACCGAACGGCGATAGTGTAGATCTCGG182760.12390447152363245No Hit
CGTTACCTTGTTACGACTTCTCCTTCCTCTAGATAACGTGATTTAATGAC182480.12371464195465337No Hit
GGAGACCTTGTTACGACTTCACCTTCCTCTAGACGATAAGGTTTGAAGAG180880.1226299015604872No Hit
AGAAACCTTGTTACGACTTTTACTTCCTCTAGATGACCAAGTTTGCCTAA180720.12252142752107056No Hit
GGAAACCTTGTTACGACTTTTATTTCCTCTAGGTGCAAAGGTTTGCTTAA180450.12233837757955504No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATAGCTTAGTTTGCCATA180280.12222312391267487No Hit
GGAAACCTTGTTACGACTTCTCCTTCCTCTAGGTGATAAGGTTTACTTAT174130.1180536530225986No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTACCCAA172080.11666382939257318No Hit
AGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTCGCCTAA171830.11649433870598472No Hit
ACAAGCAGAAGACGGGCGGTGTGTACCGAACGGCGATAGTGTAGATCTCG171260.11610789994056302No Hit
GGAAACCTTGTTACGACTTGTACTTCCTCTAAATGACCAAGTTTGACTAG169640.11500960029146975No Hit
GGAAACCTTGTTACGACTTTTACATCCTCTAAATGACCAAGTTTGACCAA169510.11492146513444373No Hit
GGCTACCTTGTTACGACTTCACCCCAGTCATGAAGCATACCGTGGTAAGC163610.11092148493095594No Hit
AGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGCCTAG154050.10444016107581297No Hit
GAAGACGGGCGGTGTGTACCGAACGGCGATAGTGTAGATCTCGGTGGTCG153870.10431812778146929No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAAATGACCAAGTTTGCCCAA147530.10001984396958577No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTACG11923150.094.966949
ACCTTGT11883050.094.9632345
CTTGTTA11890100.094.961257
CCTTGTT11908950.094.960516
TTGTTAC11902800.094.894658
AACCTTG10698300.094.847274
AAACCTT10215400.094.845623
GAAACCT10193000.094.8149952
GTAACCT441400.094.634122
GGAAACC6704250.094.555781
AGAAACC3536750.094.370121
GAGACCT200050.094.145222
GTTACCT270700.093.754162
CTACCTT596950.093.734823
TACCTTG1009500.093.480194
TGTTATG26750.093.046749
GATACCT86100.091.745062
TAACCTT462200.091.670273
TTGTGAC3650.091.0958948
GACCTTG223300.090.8094564
Produced by FastQC (version 0.11.2)